Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailEstablishment of endosymbiosis: The case of cnidarians and Symbiodinium
Fransolet, David ULg; Roberty, Stéphane ULg; Plumier, Jean-Christophe ULg

in Journal of Experimental Marine Biology and Ecology (2012), 420–421

The symbiosis between cnidarians and Symbiodinium algae (dinoflagellates) is the keystone responsible for the formation of the huge and important structures that are coral reefs. Today many environmental ... [more ▼]

The symbiosis between cnidarians and Symbiodinium algae (dinoflagellates) is the keystone responsible for the formation of the huge and important structures that are coral reefs. Today many environmental and/or anthropogenic threats compromise this tight relationship and lead to more frequent events of drastic loss of Symbiodinium pigments and eventually of algae themselves from cnidarians, better known as cnidarian bleaching. While the mechanisms underlying the collapse of the algae–coral symbiosis are progressively getting unraveled, the understanding of the mechanisms involved in the de novo infection of bleached cnidarians by Symbiodinium remains elusive. In this review, we describe the various steps needed to establish a stable symbiotic relationship between Symbiodinium and cnidarians. We review the mechanisms implicated in host–symbiont recognition and in symbiosome formation and persistence, with a special emphasis on the role played by lectins and Rab proteins. A better understanding of these molecular mechanisms may contribute to the development of strategies to promote post-bleaching recovery of corals. [less ▲]

Detailed reference viewed: 96 (11 ULg)
Full Text
Peer Reviewed
See detailEstablishment of Latency Associated with Glycoprotein E (Ge) Seroconversion after Bovine Herpesvirus 1 Infection in Calves with High Levels of Passive Antibodies Lacking Ge Antibodies
Schynts, F.; Lemaire, Mylène; Ros, C. et al

in Veterinary Microbiology (2001), 82(3), 211-22

This study was conducted to investigate the glycoprotein E (gE) antibody response raised after inoculation with a low infectious dose of bovine herpesvirus 1 (BHV-1) in six calves possessing high levels ... [more ▼]

This study was conducted to investigate the glycoprotein E (gE) antibody response raised after inoculation with a low infectious dose of bovine herpesvirus 1 (BHV-1) in six calves possessing high levels of passive immunity from cows repeatedly vaccinated with gE deleted marker vaccine. Four out of the six calves developed gE antibodies 3-5 weeks after infection, whereas the two other ones remained seronegative to gE. After 5 months of infection, the six calves were treated with dexamethasone. Virus was only re-excreted by the four calves which previously seroconverted against gE. The two other calves became seronegative against BHV-1, 30-32 weeks after infection. A second dexamethasone treatment performed 11 months after infection failed to demonstrate a latent infection in these two calves. Moreover, the lack of identification of a cell-mediated immune response, after the two dexamethasone treatments, and the failure to detect BHV-1 DNA sequences in trigeminal ganglia strongly suggest that these two calves were not latently infected. In conclusion, the presence of high levels of maternal immunity lacking gE antibodies does not prevent latency after infection with a low titre of BHV-1. Moreover, latency is associated with a serological response to gE. These results confirm that the gE deletion is a good marker to identify young calves latently infected with a field virus. [less ▲]

Detailed reference viewed: 17 (0 ULg)
Full Text
Peer Reviewed
See detail- Establishment of normal and transformed root cultures of Artemisia annua L. for artemisinin production.
Jaziri, M.; Shimomura, K.; Yoshimatsu, K. et al

in Journal of Plant Physiology (1995), 145(1-2), 175-177

Transformed cultures of Artemisia annua L. (Asteraceae) were established by the co-culture method using leaf segments of A. annua and Agrobacterium rhizogenes NCIB 8196 or MAFF 03-01724. The hairy root ... [more ▼]

Transformed cultures of Artemisia annua L. (Asteraceae) were established by the co-culture method using leaf segments of A. annua and Agrobacterium rhizogenes NCIB 8196 or MAFF 03-01724. The hairy root clones thus obtained grew vigorously on hormone-free medium, showing the typical transformed morphology. The genetic transformation of the root was proved by the opine assay. Normal root and shoot cultures were also established. A highly specific and sensitive enzyme-linked immuno-sorbent assay (ELISA) method was used for the detection and semi-quantitative determination of artemisinin and structurally related compounds in these cultures. The presence of artemisinin was confirmed by gas chromatography coupled to mass spectrometry (GC-MS) analysis. The hairy roots cultured in the dark produced no detectable level of artemisinin as shown by the adventitious shoots cultured under light conditions. The ELISA analysis of the green hairy roots cultured in liquid medium under a 16 h light/day photoperiod showed the existence of compound(s) structurally related to artemisinin, though normal and hairy roots cultured in the dark give no detectable levels of immuno-signal. [less ▲]

Detailed reference viewed: 75 (10 ULg)
Full Text
Peer Reviewed
See detailEstablishment of parathyroid hormone (PTH) reference on 10 different assay kits: impact of the recruitment of the population
CAVALIER, Etienne ULg; SOUBERBIELLE, Jean-Claude

in Osteoporosis International (2012, March), 23(Supplement 2), 360

Detailed reference viewed: 27 (2 ULg)
Full Text
Peer Reviewed
See detailThe establishment of quantifier constructions for size nouns: a diachronic case study of heap(s) and lot(s)
Brems, Lieselotte ULg

in Journal of Historical Pragmatics (2012), 2(13), 202-231

Detailed reference viewed: 31 (2 ULg)
Full Text
Peer Reviewed
See detailEstablishment of stable human fibroblast cell lines constitutively expressing active Rho-GTPases.
Servotte, S.; Zhang, Z.; Lambert, Charles ULg et al

in Protoplasma (2006), 229(2-4), 215-20

Small GTP-binding proteins of the Rho family (RhoA, Cdc42, Rac1) regulate the organisation and the turnover of the cell's cytoskeleton and adhesion structures. A significant function of these cellular ... [more ▼]

Small GTP-binding proteins of the Rho family (RhoA, Cdc42, Rac1) regulate the organisation and the turnover of the cell's cytoskeleton and adhesion structures. A significant function of these cellular structures is to translate and counterbalance forces applied to, or generated by, cells in order to maintain homeostasis and control cell movement. We therefore hypothesised that Rho-GTPases are directly involved in cellular gravity perception and may participate in the alterations induced in microgravity. To define an adequate cellular model allowing to investigate this issue, we have established stable cell lines constitutively expressing active forms of either RhoA, Cdc42, or Rac1. The three cell lines differ by morphology and by their ability to form filopodia, lamellipodia, and bundles of actin stress fibers. Overexpression of the active form of either RhoA, Cdc42, or Rac1 is compatible with cell viability and does not affect cell population doubling time. Thus, our series of mutant cells appear well suited to gain further knowledge on the molecular mechanisms of cellular gravity perception. [less ▲]

Detailed reference viewed: 48 (4 ULg)
See detailEl Estado, el mercado y la diversidad cultural
Martiniello, Marco ULg

in Aubarell, Gemma; Zapata, Ricard (Eds.) Inmigracion y proceso de cambio (2004)

Detailed reference viewed: 48 (0 ULg)
Full Text
Peer Reviewed
See detailEstado, mercado y diversidad cultural
Martiniello, Marco ULg

in Revista de Occidente (Madrid, Spain : 1923) (2003), 268

Detailed reference viewed: 50 (1 ULg)
See detail¿Estamos preparados para la ISO26000?
Parra Santamaria, Lusmiriam Andrea ULg

Conference (2011, March 17)

It is a presentation of what ISO26000 implies for the new international society and its differences with other RSC instruments.

Detailed reference viewed: 21 (1 ULg)
Peer Reviewed
See detailEstandarización de un ensayo de PCR en tiempo real para la detección y cuantificación del Virus de la Leucosis Bovina en sangre y semen de toros seropositivos
Rossich, L.; González, F.; Gutiérrez, G. et al

Poster (2008, September 21)

Detailed reference viewed: 14 (0 ULg)
Full Text
See detailEstatuto personal y sociedad multicultural: el papel de la autonomia de la voluntad
Carlier, Jean-Yves ULg

in Calvo Caravaca, A.I.; Iriarte Angel, J.L. (Eds.) Estatuto personal y multiculturalidad de la familia (2000)

Detailed reference viewed: 37 (0 ULg)
Full Text
Peer Reviewed
See detailEsterification of vertebrate like steroids in molluscs: A target of endocrine disruptors?
Giusti, Arnaud ULg; Joaquim-Justo, Célia ULg

in Comparative Biochemistry and Physiology. C : Comparative Pharmacology and Toxicology (2013), 158(4), 187-198

Alterations of the reproductive organs of gastropod molluscs exposed to pollutants have been reported in natural populations for more than 40 years. In some cases these impacts have been linked to ... [more ▼]

Alterations of the reproductive organs of gastropod molluscs exposed to pollutants have been reported in natural populations for more than 40 years. In some cases these impacts have been linked to exposure to endocrine disrupting chemicals (EDCs), which are known to induce adverse impacts on vertebrates, mainly by direct binding to steroid receptors or by altering hormone synthesis. Investigations on the mechanisms of action of endocrine disruptors in molluscs show that EDCs induce modifications of endogenous titres of androgens (e.g., testosterone, androstenedione) and oestrogens (e.g., 17ß-oestradiol). Alterations of the activity of enzymes related to steroid metabolism (i.e., cytochrome P450 aromatase, acyltransferases) are also often observed. In bivalves and gastropods, fatty acid esterification of steroids might constitute the major regulation of androgen and oestrogen homeostasis. The present review indicates that metabolism of steroid hormones to fatty acid esters might be a target of synthetic EDCs. Alterations of this process would impact the concentrations of free, potentially bioactive, form of steroids. [less ▲]

Detailed reference viewed: 39 (4 ULg)
Peer Reviewed
See detailLes esters de l’acide 6-(acétoxyméthyl)-2-oxo-2H-1-benzopyrane-3-carboxylique en tant qu’agents antiinvasifs potentiels dans le traitement du cancer
Kempen, I.; Pochet, L.; Doucet, C. et al

Conference (2000, January 28)

Detailed reference viewed: 7 (2 ULg)
Peer Reviewed
See detailEstetrol : a new natural estrogen providing a safe therapeutic window for the treatment of menopause
Gérard, Céline ULg; Gallez, Anne ULg; Silva, Elisabete et al

Poster (2015, November 14)

The increased risk of breast cancer and thromboembolism in women who take hormone replacement therapy (HRT) is a major public health problem. The discovery of new drugs with better safety profile would ... [more ▼]

The increased risk of breast cancer and thromboembolism in women who take hormone replacement therapy (HRT) is a major public health problem. The discovery of new drugs with better safety profile would provide useful advances for patient care. Estetrol (E4) is a liver friendly promising candidate for HRT. In preclinical and/or clinical studies, E4 has been effective against the main symptoms of menopause from a starting dose of 0.3 mg/kg/day. The aim of this study was to define the impact of E4 on mammary gland and breast cancer development. Our preclinical data show that E4 is less efficient than estradiol (E2) to induce mammary gland growth. Treatment with several concentrations of E4 has shown that E4 did not increase tumor development, when it is used at 0.3 mg/kg/day. However, at 3 mg/kg/day, E4 increased tumor growth similarly to E2 (0.08 mg/kg/day). E4 presents also some anti-estrogenic effects on mammary gland and antitumor activity on breast cancer by decreasing the strong proliferative effect of E2. While ERα is the predominant receptor mediating its effects, the dual weak-estrogenic/anti-estrogenic feature of E4 results from differential signaling pathways activation. Both nuclear and rapid extra-nuclear signaling pathways are necessary for a complete estrogenic effect of E4. However, the antitumor action of E4 is not due to a capacity to antagonize E2-induced nuclear activity. In conclusion, our results support that E4, if it is used in strictly controlled clinical applications, could have no or only limited impact on breast and breast cancer. [less ▲]

Detailed reference viewed: 47 (3 ULg)