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See detailFast F-18 FDG synthesis by alkaline hydrolysis on a low polarity solid phase supports
Lemaire, Christian ULg; Damhaut, P.; Lauricella, Benjamino ULg et al

in Journal of Labelled Compounds & Radiopharmaceuticals (2002), 45(5), 435-447

The synthesis of 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) has been simplified by the use of a tC18 Sep Pak cartridge to effect purification and hydrolysis of the tetraacetylated [18F]fluoro-glucose ... [more ▼]

The synthesis of 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) has been simplified by the use of a tC18 Sep Pak cartridge to effect purification and hydrolysis of the tetraacetylated [18F]fluoro-glucose compound ([18F]TAG). After radiolabelling, this derivative was trapped on a solid phase extraction (SPE) cartridge and the residual reaction solvent (CH3CN), reagents (K222, K2CO3,) and by-products removed by washing the support with water. After this cleaning step, the acetyl groups were cleaved on the same tC18 column using 2N sodium hydroxide. This fast reaction proceeded near quantitatively (>98%) at room temperature in less than 2 min. The [18F]FDG was then recovered with a small amount of water, neutralized with a slight excess of 2N hydrochloric acid, buffered for pH with a citrate solution and finally purified on a neutral alumina oxide and a second tC18 column. After filtration, the radiochemical yield of this [18F]FDG isotonic solution after more than 100 production runs was found to be very reliable and reproducible (70±6% decay corrected). The synthesis time was about 22 min. Quality controls showed that the radiochemical purity was higher than 98% and in any case no [18F]FDM was detected. Only traces of 2-chloro-2-deoxy-glucose (ClDG) were found in the final sample (64±9 g/ batch of 16 ml). [18F]FDG specific activity averaged between 1 and 20 Ci/µmol (EOS). No evaporation and use of ion retardation resin (AG11A8) are required. Moreover, this new approach is suitable for complete remote operation using available single use medical components. Copyright © 2002 John Wiley [less ▲]

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See detailFast freezing of cow embryos in French straws with an automatic program.
Massip, A.; Van der Zwalmen, P.; Hanzen, Christian ULg et al

in Theriogenology (1982), 18(3), 325-332

Cow embryos between day 6.5 and 9 were frozen in 1.5M DMSO in PBS at 2 degrees C/min from seeding to -25 degrees C before being plunged into liquid nitrogen directly or after 10 min at -25 degrees C ... [more ▼]

Cow embryos between day 6.5 and 9 were frozen in 1.5M DMSO in PBS at 2 degrees C/min from seeding to -25 degrees C before being plunged into liquid nitrogen directly or after 10 min at -25 degrees C. Cooling rate from 20 degrees C to -5 degrees C was 9 degrees C/min. Seeding was induced automatically at -5 degrees C by injection of liquid nitrogen vapour. Embryos were subsequently thawed by direct transfer to water at 20 degrees C (group I) or at 37 degrees C (group II). Survival was assessed by culture in vitro and by transfer. In group I, 35.7% were degenerated after thawing (compared to 35.4% in group II). Survival rate after culture in vitro for 24h was not significantly different (48.3% vs 42.8%) and hatching rate after 96h culture was quite similar (33.3% vs 34.4%). In group II, four pregnancies were obtained from 10 embryos transferred. Time at -25 degrees C did not improve the results. Automatic seeding did not impair survival. These results show that the quality of the embryo is the determinant factor for survival after freezing and that the plastic straw is the most suitable vessel for freezing, storage and transfer of embryos. [less ▲]

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See detailA fast gas chromatographic method for the study of semiochemical slow release formulations
Heuskin, Stéphanie ULg; Lorge, Stéphanie; Leroy, Pascal ULg et al

Poster (2010, January)

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See detailFast gas-phase hydrogen/deuterium exchange observed for a DNA G-quadruplex
Gabelica, Valérie ULg; Rosu, Frédéric ULg; Witt, Matthias et al

in Rapid Communications in Mass Spectrometry : RCM (2005), 19(2), 201-208

The gas-phase hydrogen/deuterium (H/D) exchange kinetics of DNA G-quadruplexes has been investigated using Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS). The quadruplex [(TGGGGT)(4 ... [more ▼]

The gas-phase hydrogen/deuterium (H/D) exchange kinetics of DNA G-quadruplexes has been investigated using Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS). The quadruplex [(TGGGGT)(4).3NH(4)(+)] undergoes very fast H/D exchange, in both the positive and in the negative ion modes, compared to DNA duplexes and other quadruplexes tested, and compared to the corresponding single-stranded TGGGGT. Substitution of NH4+ for K+ did not alter this fast H/D exchange, indicating that the hydrogens of the ammonium ions are not those exchanged. However, stripping of the interior cations of the quadruplex by source collision-induced dissociation (CID) in the positive ion mode showed that the presence of the inner cations is essential for the fast exchange to be possible. Molecular dynamics simulations show that the G-quadruplex is very rigid in the gas phase with NH4+ ions inside the tetrads. We suggest that the fast H/D exchange is favored by this rigid quadruplex conformation. This example illustrates that the concept that compact DNA structures exchange H for D slower than unfolded ones is a misconception. Copyright (C) 2004 John Wiley Sons, Ltd. [less ▲]

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See detailFast Homozygosity Mapping and Identification of a Zebrafish ENU-Induced Mutation by Whole-Genome Sequencing.
Voz, Marianne ULg; Coppieters, Wouter ULg; Manfroid, Isabelle ULg et al

in PLoS ONE (2012), 7(4), 34671

Forward genetics using zebrafish is a powerful tool for studying vertebrate development through large-scale mutagenesis. Nonetheless, the identification of the molecular lesion is still laborious and ... [more ▼]

Forward genetics using zebrafish is a powerful tool for studying vertebrate development through large-scale mutagenesis. Nonetheless, the identification of the molecular lesion is still laborious and involves time-consuming genetic mapping. Here, we show that high-throughput sequencing of the whole zebrafish genome can directly locate the interval carrying the causative mutation and at the same time pinpoint the molecular lesion. The feasibility of this approach was validated by sequencing the m1045 mutant line that displays a severe hypoplasia of the exocrine pancreas. We generated 13 Gb of sequence, equivalent to an eightfold genomic coverage, from a pool of 50 mutant embryos obtained from a map-cross between the AB mutant carrier and the WIK polymorphic strain. The chromosomal region carrying the causal mutation was localized based on its unique property to display high levels of homozygosity among sequence reads as it derives exclusively from the initial AB mutated allele. We developed an algorithm identifying such a region by calculating a homozygosity score along all chromosomes. This highlighted an 8-Mb window on chromosome 5 with a score close to 1 in the m1045 mutants. The sequence analysis of all genes within this interval revealed a nonsense mutation in the snapc4 gene. Knockdown experiments confirmed the assertion that snapc4 is the gene whose mutation leads to exocrine pancreas hypoplasia. In conclusion, this study constitutes a proof-of-concept that whole-genome sequencing is a fast and effective alternative to the classical positional cloning strategies in zebrafish. [less ▲]

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See detailFast mapping between grammatical constructions and meaning Two experiments in French children aged 3 to 4
Maillart, Christelle ULg; Parisse, Christophe; Dupont, Fanny et al

Poster (2008)

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See detailFast mapping between grammatical constructions and meaning: An experiment in French children aged 3 to 4
Parisse, Christophe; Dupont, Fanny; Bourdoux, Françoise et al

Poster (2008, June)

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See detailFast method for the simultaneous quantification of toxic polyphenols applied to the selection of genotypes of yam bean (Pachyrhizus sp.) seeds.
Lautié, Emmanuelle; Rozet, Eric ULg; Hubert, Philippe ULg et al

in Talanta (2013), 117

The purpose of the research was to develop and validate a rapid quantification method able to screen many samples of yam bean seeds to determine the content of two toxic polyphenols, namely pachyrrhizine ... [more ▼]

The purpose of the research was to develop and validate a rapid quantification method able to screen many samples of yam bean seeds to determine the content of two toxic polyphenols, namely pachyrrhizine and rotenone. The analytical procedure described is based on the use of an internal standard (dihydrorotenone) and is divided in three steps: microwave assisted extraction, purification by solid phase extraction and assay by ultra high performance liquid chromatography (UHPLC). Each step was included in the validation protocol and the accuracy profiles methodology was used to fully validate the method. The method was fully validated between 0.25mg and 5mg pachyrrhizin per gram of seeds and between 0.58mg/g and 4mg/g for rotenone. More than one hundred samples from different accessions, locations of growth and harvest dates were screened. Pachyrrhizine concentrations ranged from 3.29mg/g to lower than 0.25mg/g while rotenone concentrations ranged from 3.53mg/g to lower than 0.58mg/g. This screening along with principal component analysis (PCA) and discriminant analysis (DA) analyses allowed the selection of the more interesting genotypes in terms of low concentrations of these two toxic polyphenols. [less ▲]

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See detailFast method to predict an earing profile based on Lankford coefficients and yield locus
Lelotte, Thomas; Duchene, Laurent ULg; Habraken, Anne ULg

in Mota Soares, C. A. (Ed.) Proceedings of the III European Conference on Computational Mechanics Solids (ECCM III) (2006)

This paper compares three methods to determine the earing profile that appears during the deep-drawing test of a circular steel sheet. Three steel grades will be studied. The first method is an ... [more ▼]

This paper compares three methods to determine the earing profile that appears during the deep-drawing test of a circular steel sheet. Three steel grades will be studied. The first method is an experimental test made by a hydraulic press. The second one is a finite ele-ment method (FEM) deep-drawing simulation using a micro-macro texture based constitutive law. The last one directly determines the general aspect of the earing profile from the yield locus which is computed from the initial texture. The goal of this study is to validate the last approach. So, an earing profile prediction can quickly be obtained just from material’s tex-ture. [less ▲]

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See detailA Fast Method To Predict Protein Interaction Sites From Sequences
Gallet, X.; Charloteaux, Benoît ULg; Thomas, Annick ULg et al

in Journal of Molecular Biology (2000), 302(4), 917-26

A simple method for predicting residues involved in protein interaction sites is proposed. In the absence of any structural report, the procedure identifies linear stretches of sequences as "receptor ... [more ▼]

A simple method for predicting residues involved in protein interaction sites is proposed. In the absence of any structural report, the procedure identifies linear stretches of sequences as "receptor-binding domains" (RBDs) by analysing hydrophobicity distribution. The sequences of two databases of non-homologous interaction sites eliciting various biological activities were tested; 59-80 % were detected as RBDs. A statistical analysis of amino acid frequencies was carried out in known interaction sites and in predicted RBDs. RBDs were predicted from the 80,000 sequences of the Swissprot database. In both cases, arginine is the most frequently occurring residue. The RBD procedure can also detect residues involved in specific interaction sites such as the DNA-binding (95 % detected) and Ca-binding domains (83 % detected). We report two recent analyses; from the prediction of RBDs in sequences to the experimental demonstration of the functional activities. The examples concern a retroviral Gag protein and a penicillin-binding protein. We support that this method is a quick way to predict protein interaction sites from sequences and is helpful for guiding experiments such as site-specific mutageneses, two-hybrid systems or the synthesis of inhibitors. [less ▲]

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See detailFast microwave assisted extraction of rotenone for its quantification in seeds of yam bean (Pachyrhizus sp.)
Lautié, E.; Rasse, C.; Rozet, Eric ULg et al

in Journal of Separation Science (2013), 36

The aim of this study was to find if fast microwave assisted extraction could be an alternative to the conventional soxhlet extraction for the quantification of rotenone in yam bean seeds by solid phase ... [more ▼]

The aim of this study was to find if fast microwave assisted extraction could be an alternative to the conventional soxhlet extraction for the quantification of rotenone in yam bean seeds by solid phase extraction and HPLC-UV. For this purpose, an experimental design was used to determine the optimal conditions of the microwave extraction. Then the values of the quantification on three accessions from two different species of yam bean seeds were compared using the two different kinds of extraction. A microwave extraction of 11 min at 55°C using methanol/dichloromethane (50:50) allowed rotenone extraction either equivalently or more efficiently than the 8h soxhlet extraction method and was less sensitive to moisture content. The selectivity, precision, trueness, accuracy and limit of quantification of themethod with microwave extraction were also demonstrated. [less ▲]

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See detailFast MR arthrography using VIBE sequences to evaluate the rotator cuff
Vandevenne, J. E.; Vanhoenacker, F.; Mahachie John, Jestinah ULg et al

in Skeletal Radiology (2009), 38(7), 669-74

PURPOSE: The purpose of this paper was to evaluate if short volumetric interpolated breath-hold examination (VIBE) sequences can be used as a substitute for T1-weighted with fat saturation (T1-FS ... [more ▼]

PURPOSE: The purpose of this paper was to evaluate if short volumetric interpolated breath-hold examination (VIBE) sequences can be used as a substitute for T1-weighted with fat saturation (T1-FS) sequences when performing magnetic resonance (MR) arthrography to diagnose rotator cuff tears. MATERIALS AND METHODS: Eighty-two patients underwent direct MR arthrography of the shoulder joint using VIBE (acquisition time of 13 s) and T1-FS (acquisition time of 5 min) sequences in the axial and paracoronal plane on a 1.0-T MR unit. Two radiologists scored rotator cuff tendons on VIBE and T1-FS images separately as normal, small/large partial thickness and full thickness tears with or without geyser sign. T1-FS sequences were considered the gold standard. Surgical correlation was available in a small sample. RESULTS: Sensitivity, specificity, and positive and negative predictive values of VIBE were greater than 92% for large articular-sided partial thickness and full thickness tears. For detecting fraying and articular-sided small partial thickness tears, these parameters were 55%, 94%, 94%, and 57%, respectively. The simple kappa value was 0.76, and the weighted kappa value was 0.86 for agreement between T1-FS and VIBE scores. All large partial and full thickness tears at surgery were correctly diagnosed using VIBE or T1-FS MR images. CONCLUSION: Fast MR arthrography of the shoulder joint using VIBE sequences showed good concordance with the classically used T1-FS sequences for the appearance of the rotator cuff, in particular for large articular-sided partial thickness tears and for full thickness tears. Due to its very short acquisition time, VIBE may be especially useful when performing MR arthrography in claustrophobic patients or patients with a painful shoulder. [less ▲]

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See detailFast Multi-Class Image Annotation with Random Subwindows and Multiple Output Randomized Trees
Dumont, Marie; Marée, Raphaël ULg; Wehenkel, Louis ULg et al

in Proc. International Conference on Computer Vision Theory and Applications (VISAPP) (2009, February)

This paper addresses image annotation, i.e. labelling pixels of an image with a class among a finite set of predefined classes. We propose a new method which extracts a sample of subwindows from a set of ... [more ▼]

This paper addresses image annotation, i.e. labelling pixels of an image with a class among a finite set of predefined classes. We propose a new method which extracts a sample of subwindows from a set of annotated images in order to train a subwindow annotation model by using the extremely randomized trees ensemble method appropriately extended to handle high-dimensional output spaces. The annotation of a pixel of an unseen image is done by aggregating the annotations of its subwindows containing this pixel. The proposed method is compared to a more basic approach predicting the class of a pixel from a single window centered on that pixel and to other state-of-the-art image annotation methods. In terms of accuracy, the proposed method significantly outperforms the basic method and shows good performances with respect to the state-of-the-art, while being more generic, conceptually simpler, and of higher computational efficiency than these latter. [less ▲]

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See detailFast multipole accelerated finite element-boundary element analysis of shielded induction heaters
V Sabariego, Ruth ULg; Sergeant, Peter; Gyselinck, Johan et al

in IEEE Transactions on Magnetics (2006), 42(4), 1407-1410

This paper deals with the analysis of a shielded induction heater by means of a fast multipole accelerated hybrid finite-element boundary-element model. It concerns an experimental setup with passive and ... [more ▼]

This paper deals with the analysis of a shielded induction heater by means of a fast multipole accelerated hybrid finite-element boundary-element model. It concerns an experimental setup with passive and active shielding for mitigating the stray field in the surrounding area. Numerical results of the magnetodynamic model are compared with measurements. Further, various aspects of the numerical scheme are discussed and its efficiency is evidenced. [less ▲]

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See detailFast multipole acceleration of the hybrid finite-element/boundary-element analysis of 3-D eddy-current problems
V Sabariego, Ruth ULg; Gyselinck, J.; Dular, Patrick ULg et al

in Proceedings of the 12th COMPUMAG Conference on the Computation of Electromagnetic Fields (2003)

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See detailFast multipole acceleration of the hybrid finite-element/boundary-element analysis of 3-D eddy-current problems
V Sabariego, Ruth ULg; Gyselinck, J.; Dular, Patrick ULg et al

in IEEE Transactions on Magnetics (2004), 40(2), 1278-1281

This paper deals with the acceleration of the hybrid finite-element/boundary-element analysis of three-dimensional (3-D) eddy-current problems by means of the fast multipole method. An adaptive truncation ... [more ▼]

This paper deals with the acceleration of the hybrid finite-element/boundary-element analysis of three-dimensional (3-D) eddy-current problems by means of the fast multipole method. An adaptive truncation scheme for the multipole expansion of the 3-D Laplace Green function is proposed. As an application example, the TEAM workshop problem 28 is considered. The computational cost without and with fast multipole acceleration is discussed. [less ▲]

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See detailFast multiresponsive micellar gels from a smart ABC triblock copolymer
Willet, Nicolas ULg; Gohy, Jean-François; Lei, Liangcai et al

in Angewandte Chemie (International ed. in English) (2007), 46(42), 7988-7992

The triblock copolymer PS-b-P2VP-b-PEO undergoes simultaneous micellization and gelation, leading to high-storage-modulus materials that have fast responses to pH value, temperature, ionic strength, and ... [more ▼]

The triblock copolymer PS-b-P2VP-b-PEO undergoes simultaneous micellization and gelation, leading to high-storage-modulus materials that have fast responses to pH value, temperature, ionic strength, and shearing. The gel has a hierarchical structure with spherical core-shell-corona micelles, which, in turn, pack closely into an ordered cubic structure. [less ▲]

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