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See detailEnhanced Multivariate Analysis of Mainstream Tobacco Smoke Particulate Phase by HS-SPME-GC×GC-TOFMS
Brokl, Michal ULg; Bishop, L; Wright, C et al

Conference (2014, May 19)

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See detailEnhanced neural activation in brain regions mediating sexual responses following exposure to a conditioned stimulus that predicts copulation.
Taziaux, Mélanie ULg; Kahn, A.; Moore, J. et al

in Neuroscience (2008), 151(3), 644-58

Stimuli associated with sexual behavior increase reproductive success if presented prior to copulation. In Japanese quail, inseminations that take place in a context that predicts the arrival of a female ... [more ▼]

Stimuli associated with sexual behavior increase reproductive success if presented prior to copulation. In Japanese quail, inseminations that take place in a context that predicts the arrival of a female are more likely to result in fertilized eggs. We demonstrate here that in male Japanese quail a sexual conditioned stimulus (CS) also enhances activity in two brain regions that mediate sexual behavior, the medial preoptic area and the medial part of the bed nucleus of the stria terminalis. C-fos expression, a marker of neural activation, was higher in these areas in subjects exposed sequentially to a sexual CS and copulation than in subjects exposed to copulation or the CS alone or in subjects exposed to no sexual stimulus, either an identical, untrained CS or an empty arena. These results suggest a link between a proximate result of sexual CS presentation, male brain activation, and a known ultimate outcome, increased fertilizations. [less ▲]

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See detailEnhanced neutralizing activity of anti-BVD monoclonal antibodies with anti-mouse Ig goat serum or when some Mabs are mixed
Boulanger, D.; Waxweiler, S.; Karelle-Bui, L. et al

Poster (1990)

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See detailEnhanced nutritionally induced adipose tissue development in mice with stromelysin-1 gene inactivation.
Maquoi, Erik ULg; Demeulemeester, Diego; Vörös, Gabor et al

in Thrombosis and Haemostasis (2003), 89(4), 696-704

To investigate a potential role of stromelysin-1 (MMP-3) in development of adipose tissue, 5 week old male MMP-3 deficient mice (MMP-3(-/-)) and wild-type (MMP-3(+/+)) controls were kept on a high fat ... [more ▼]

To investigate a potential role of stromelysin-1 (MMP-3) in development of adipose tissue, 5 week old male MMP-3 deficient mice (MMP-3(-/-)) and wild-type (MMP-3(+/+)) controls were kept on a high fat diet (HFD) for 15 weeks. MMP-3(-/-) mice were hyperphagic and gained more weight than the MMP-3(+/+) mice. At the time of sacrifice, the body weight of the MMP-3(-/-) mice was significantly higher than that of the MMP-3(+/+) mice, as was the weight of the isolated subcutaneous (SC) and gonadal (GON) fat deposits. Significant adipocyte hypertrophy was observed in the GON but not in the SC adipose tissue of MMP-3(-/-) mice. Fasting plasma glucose and cholesterol levels were comparable in both genotypes, whereas triglyceride levels were significantly lower in MMP-3(-/-) mice. Staining with an endothelial cell specific lectin revealed a significantly higher blood vessel density and larger total stained area in the GON adipose tissues of MMP-3(-/-) mice. Thus, in a murine model of nutritionally induced obesity, MMP-3 impairs adipose tissue development, possibly by affecting food intake and/or adipose tissue-related angiogenesis. [less ▲]

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See detailEnhanced ocean carbon storage from anaerobic alkalinity generation in coastal sediments
Thomas, H.; Schiettecatte, L.-S.; Suykens, Kim ULg et al

in Biogeosciences (2009), 6

The coastal ocean is a crucial link between land, the open ocean and the atmosphere. The shallowness of the water column permits close interactions between the sedimentary, aquatic and atmospheric ... [more ▼]

The coastal ocean is a crucial link between land, the open ocean and the atmosphere. The shallowness of the water column permits close interactions between the sedimentary, aquatic and atmospheric compartments, which otherwise are decoupled at long time scales ( =1000 yr) in the open oceans. Despite the prominent role of the coastal oceans in absorbing atmospheric CO2 and transferring it into the deep oceans via the continental shelf pump, the underlying mechanisms remain only partly understood. Evaluating observations from the North Sea, a NW European shelf sea, we provide evidence that anaerobic degradation of organic matter, fuelled from land and ocean, generates total alkalinity (AT) and increases the CO2 buffer capacity of seawater. At both the basin wide and annual scales anaerobic AT generation in the North Sea’s tidal mud flat area irreversibly facilitates 7–10%, or taking into consideration benthic denitrification in the North Sea, 20–25% of the North Sea’s overall CO2 uptake. At the global scale, anaerobic AT generation could be accountable for as much as 60% of the uptake of CO2 in shelf and marginal seas, making this process, the anaerobic pump, a key player in the biological carbon pump. Under future high CO2 conditions oceanic CO2 storage via the anaerobic pump may even gain further relevance because of stimulated ocean productivity. [less ▲]

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See detailEnhanced ocean temperature forecast skills through 3-D super-ensemble multi-model fusion
Lenartz, Fabian ULg; Mourre, B.; Barth, Alexander ULg et al

in Geophysical Research Letters (2010), 37(L19606),

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See detailEnhanced or Reduced Fetal Growth Induced by Embryo Transfer into Smaller or Larger Breeds Alters Post-Natal Growth and Metabolism in Pre-Weaning Horses
Peugnet, Pauline; Wimel, Laurence; Duchamp, Guy et al

in PLoS ONE (2014), 9(7), 102044

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See detailEnhanced osteoclast development in collagen-induced arthritis in interferon-gamma receptor knock-out mice as related to increased splenic CD11b(+) myelopoiesis
De Klerck, Bert; Carpentier, Isabelle; Lories, Rick J et al

in Arthritis Research & therapy (2004), 6(3), 220-231

Collagen-induced arthritis (CIA) in mice is accompanied by splenomegaly due to the selective expansion of immature CD11b(+) myeloblasts. Both disease manifestations are more pronounced in interferon-gamma ... [more ▼]

Collagen-induced arthritis (CIA) in mice is accompanied by splenomegaly due to the selective expansion of immature CD11b(+) myeloblasts. Both disease manifestations are more pronounced in interferon-gamma receptor knock-out (IFN-gammaR KO) mice. We have taken advantage of this difference to test the hypothesis that the expanding CD11b(+) splenic cell population constitutes a source from which osteoclast precursors are recruited to the joint synovia. We found larger numbers of osteoclasts and more severe bone destruction in joints of IFN-gammaR KO mice than in joints of wild-type mice. Osteoclast-like multinucleated cells appeared in splenocyte cultures established in the presence of macrophage colony-stimulating factor (M-CSF) and stimulated with the osteoclast-differentiating factor receptor activator of NF-kappaB ligand (RANKL) or with tumour necrosis factor-alpha (TNF-alpha). Significantly larger numbers of such cells could be generated from splenocytes of IFN-gammaR KO mice than from those of wildtype mice. This was not accompanied, as might have been expected, by increased concentrations of the intracellular adaptor protein TRAF6, known to be involved in signalling of RANKL- and TNF-alpha-induced osteoclast formation. Splenocyte cultures of IFN-gammaR KO mice also produced more TNF-alpha and more RANKL than those of wild-type mice. Finally, splenocytes isolated from immunised IFN-gammaR KO mice contained comparatively low levels of pro-interleukin-1beta (pro-IL-1beta) and pro-caspase-1, indicating more extensive conversion of pro-IL-1beta into secreted active IL-1beta. These observations provide evidence that all conditions are fulfilled for the expanding CD11b(+) splenocytes to act as a source of osteoclasts and to be indirectly responsible for bone destruction in CIA. They also provide a plausible explanation for the higher susceptibility of IFN-gammaR KO mice to CIA. [less ▲]

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See detailEnhanced pinning and proliferation of matching effects in a superconducting film with a Penrose array of magnetic dots
Silhanek, Alejandro ULg; Gillijns, W.; Moshchalkov, V. V. et al

in Applied Physics Letters (2006), 89(15),

The vortex dynamics in superconducting films deposited on top of a fivefold Penrose array of magnetic dots is studied by means of transport measurements. The authors show that in the low pinning regime ... [more ▼]

The vortex dynamics in superconducting films deposited on top of a fivefold Penrose array of magnetic dots is studied by means of transport measurements. The authors show that in the low pinning regime (demagnetized dots) a few periodic and aperiodic matching features coexist. In the strong pinning regime (magnetized dots) a richer structure of unforeseen periodic and aperiodic vortex patterns appear, giving rise to a clear enhancement of the critical current in a broader field range. Possible stable vortex configurations are determined by molecular dynamics simulations. (c) 2006 American Institute of Physics. [less ▲]

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See detailEnhanced pinning in superconducting thin films with graded pinning landscapes
Motta, M.; Colauto, F.; Ortiz, W.A. et al

in Applied Physics Letters (2013), 102

A graded distribution of antidots in superconducting a-Mo79Ge21 thin films has been investigated by magnetization and magneto-optical imaging measurements. The pinning landscape has maximum density at the ... [more ▼]

A graded distribution of antidots in superconducting a-Mo79Ge21 thin films has been investigated by magnetization and magneto-optical imaging measurements. The pinning landscape has maximum density at the sample border, decreasing linearly towards the center. Its overall performance is noticeably superior than that for a sample with uniformly distributed antidots: For high temperatures and low fields, the critical current is enhanced, whereas the region of thermomagnetic instabilities in the field-temperature diagram is significantly suppressed. These findings confirm the relevance of graded landscapes on the enhancement of pinning efficiency, as recently predicted by Misko and Nori [Phys. Rev. B 85, 184506 (2012)]. [less ▲]

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See detailEnhanced Platelet Reactivity to Collagen and Shear Stress in Transgenic Mice Overexpressing the Platelet P2X1 Ion Channel
Oury, Cécile ULg; Kuijpers, Marijke; Toth-Zsamboki, Emese et al

in Blood (2002), 100

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See detailAn enhanced process for the production of a highly purified extracellular lipase in the non-conventional yeast Yarrowia lipolytica
Turki, S.; Ayed, A.; Chalghoumi, N. et al

in Applied Biochemistry and Biotechnology (2010), 160(5), 1371-1385

Yarrowia lipolytica LgX64.81 is a non-genetically modified mutant that was previously identified as a promising microorganism for extracellular lipase production. In this work, the development of a fed ... [more ▼]

Yarrowia lipolytica LgX64.81 is a non-genetically modified mutant that was previously identified as a promising microorganism for extracellular lipase production. In this work, the development of a fed-batch process for the production of this enzyme in this strain was described. A lipolytic activity of 2,145 U/mL was obtained after 32 h of batch culture in a defined medium supplemented with 10 g/L of tryptone, an enhancer of lipase expression. To maximize the volumetric productivity, two different fed-batch strategies had been investigated. In comparison to batch process, the intermittent fed-batch strategy had not improved the volumetric lipase productivity. In contrast, the stepwise feeding strategy combined with uncoupled cell growth and lipase production phases resulted in a 2-fold increase in the volumetric lipase productivity, namely, the lipase activity reached 10,000 U/mL after 80 h of culture. Furthermore, this lipase was purified to homogeneity by anion exchange chromatography on MonoQ resin followed by gel filtration on Sephacryl S-100. This process resulted in an overall yield of 72% and a 3.5-fold increase of the specific lipase activity. The developed process offers a great potential for an economic production of Lip2 at large scale in Y. lipolytica LgX64.81. © 2009 Humana Press. [less ▲]

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See detailEnhanced proof strength after cold process of fabrication of non-linear metallic profiles – Comparison of two predictive models for hollow sections
Rossi, Barbara ULg; Jaspart, Jean-Pierre ULg

in International Symposium on Tubular Structures : ISTS13, Hong Kong, 15-17 December 2010 (2010)

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See detailEnhanced Release of Plasminogen Activator Inhibitor(S) but Not of Plasminogen Activators by Cultured Rat Glial Cells Treated with Interleukin-1
Rogister, Bernard ULg; Leprince, Pierre ULg; Delree, P. et al

in Glia (1990), 3(4), 252-7

Astroglial cells are known to proliferate during development of the nervous system, as well as during post-traumatic gliosis. We have previously shown that the proliferation of cultured astrocytes can be ... [more ▼]

Astroglial cells are known to proliferate during development of the nervous system, as well as during post-traumatic gliosis. We have previously shown that the proliferation of cultured astrocytes can be stimulated by the urokinase-type (uPA) of plasminogen activator (PA) and that astrocytes are able to release such uPA upon stimulation with basic fibroblast growth factor, which is known to act as a mitogen for these cells. Here we report studies on the effects of human interleukin-1 (IL-1) on the release of PA activity by cultured newborn rat astroglial cells. Whereas there is controversy in the literature as to whether IL-1 stimulates multiplication of astroglial cells, we failed to observe such an effect in our system. We did observe, however, a dose-dependent decrease in PA activity in the supernatant of the IL-1 treated cultures. Further analysis revealed that this apparent decrease in PA release was in fact due to an increased release of plasminogen activator inhibitor (PAI). A similar IL-1 induced increase in PAI release was also found to occur in cultures of transformed astrocytes (human glioma LN18) and in cultured Schwann cells, but not in cultures of neurons or neuronal tumour cells. Since protease inhibitors are known to possess neuritogenic properties, our results suggest that IL-1, by its capacity to induce PAI, may promote neuritogenesis. [less ▲]

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See detailEnhanced silicate weathering of tropical shelf sediments exposed during glacial lowstands: a sink for atmospheric CO2
Wan, Shiming; Clift, Peter D.; Zhao, Debo et al

in Geochimica et Cosmochimica Acta (2017), 200

Atmospheric CO2 and global climate are closely coupled. Since 800 ka CO2 concentrations have been up to 50% higher during interglacial compared to glacial periods. Because of its dependence on temperature ... [more ▼]

Atmospheric CO2 and global climate are closely coupled. Since 800 ka CO2 concentrations have been up to 50% higher during interglacial compared to glacial periods. Because of its dependence on temperature, humidity, and erosion rates, chemical weathering of exposed silicate minerals was suggested to have dampened these cyclic variations of atmospheric composition. Cooler and drier conditions and lower non-glacial erosion rates suppressed in situ chemical weathering rates during glacial periods. However, using systematic variations in major element geochemistry, Sr-Nd isotopes and clay mineral records from Ocean Drilling Program Sites 1143 and 1144 in the South China Sea spanning the last 1.1 Ma, we show that sediment deposited during glacial periods was more weathered than sediment delivered during interglacials. We attribute this to subaerial exposure and weathering of unconsolidated shelf sediments during glacial sealevel lowstands. Our estimates suggest that enhanced silicate weathering of tropical shelf sediments exposed during glacial lowstands can account for ~9% of the carbon dioxide removed from the atmosphere during the glacial and thus represent a significant part of the observed glacial-interglacial variation of ~80 ppmv. As a result, if similar magnitudes can be identified in other tropical shelf-slope systems, the effects of increased sediment exposure and subsequent silicate weathering during lowstands could have potentially enhanced the drawdown of atmospheric CO2 during cold stages of the Quaternary. This in turn would have caused an intensification of glacial cycles. We attribute this to subaerial exposure and weathering of unconsolidated shelf sediments during glacial sealevel lowstands. Our estimates suggest that enhanced silicate weathering of tropical shelf sediments exposed during glacial lowstands can account for ~9% of the carbon dioxide removed from the atmosphere during the glacial and thus represent a significant part of the observed glacial-interglacial variation of ~80 ppmv. As a result, if similar magnitudes can be identified in other tropical shelf-slope systems, the effects of increased sediment exposure and subsequent silicate weathering during lowstands could have potentially enhanced the drawdown of atmospheric CO2 during cold stages of the Quaternary. This in turn would have caused an intensification of glacial cycles. [less ▲]

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See detailEnhanced sludge dewatering and drying comparison of two linear polyelectrolytes co-conditioning with Polyaluminium chloride
Pambou, Yvon-Bert ULg; Fraikin, Laurent ULg; Salmon, Thierry ULg et al

Poster (2014, May 26)

Annual production of sewage sludge in Europe is estimated at more than eleven million tons of dry matter content [1]. Use in agriculture and incineration are the main ways of valorization. In this context ... [more ▼]

Annual production of sewage sludge in Europe is estimated at more than eleven million tons of dry matter content [1]. Use in agriculture and incineration are the main ways of valorization. In this context, drying of residual sludge appears as an essential step after mechanical dewatering. It reduces the costs of storage, transport and allows the sludge stabilization. However, this process is highly energy consuming and still needs to be optimized as it constitutes an important economic and environmental issue [2]. Polymers are usually employed in the conditioning step in order to promote particle aggregation, making the dewatering easier. The past decades, the application of pre-hydrolized polyaluminium chlorides (PACls) as coagulant has increased, particularly in China, Japan, Russia and Western Europe [3]. As a consequence, PACls are extensively investigated for their coagulation performance, characterization and speciation [3]. In this work, the influence of Polyaluminium chloride (PAX-14) co-conditioning with linear polyelectrolytes on sludge dewatering and drying performances was investigated. Experiments were conducted on activated sludge samples collected after thickening from the wastewater treatment plant of the Grosses- Battes (Belgium). Two cationic polymers were tested, each of them in combination with PAX for sludge flocculation prior to mechanical dewatering and their effects on sludge convective drying. The one referenced as 640 LH was a linear polymer with a low molecular weight, whereas the 640 CT was a linear one with a high molecular weight. After conditioning, the dewatering step was realized by using a normalized filtration-expression cell (AFNOR 1979) under 5 bar of pressure. Then, the specific resistance to filtration was determined from the follow-up of filtrate mass with time, using the Carman-Kozeny equation [4]. Figure 1 shows the experimental design. For convective drying experiments, the cake recovered after filtration was extruded through a circular die of 14 mm diameter and cut at a height of 14 mm, yielding cylindrical samples with mass of approximately 2.5 g, as used in several industrial belt dryers. Individual extrudates have been dried in a specially designed convective micro-dryer. Results reported in this paper were obtained with the following operating conditions: air temperature of 130 °C, at ambient humidity (absolute humidity ~ 0.005 kgwater/ kgdry air) and a superficial velocity of 1 m/ s. Results show that, the dry solids content of the dewatering cake increases with increasing Polyaluminium chloride dosage for both series of experiments. It supposes that PAX contributed to improve solids capture, depicted on Fig. 2. Concerning drying, it appeared that the samples treated by the dual PAX/ polymers combination showed higher drying rates than samples conditioned by polymer without PAX addition, allowing a reduction of the drying time (see Fig. 3). Table 1 shows the drying characteristics of the samples. The drying time decreases and the average drying rate increases with the dual conditioners addition, while the initial water content decreases as well as the total amount water to be removed. [less ▲]

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See detailEnhanced sludge dewatering and drying comparison of two linear polyelectrolytes co-conditioning with Polyaluminium chloride
Pambou, Yvon-Bert ULg; Fraikin, Laurent ULg; Salmon, Thierry ULg et al

Poster (2014, October 23)

Annual production of sewage sludge in Europe is estimated at more than ten million tons of dry matter. Use in agriculture and incineration are the main ways of valorization. In this context, sludge drying ... [more ▼]

Annual production of sewage sludge in Europe is estimated at more than ten million tons of dry matter. Use in agriculture and incineration are the main ways of valorization. In this context, sludge drying appears as an essential step after mechanical dewatering. It reduces the costs of storage and transport, allows the stabilization and the hygienization of sludge while increasing its calorific value. However, this process is highly energy consuming and still needs to be optimized as it constitutes an important economic and environmental issue. This implies the improvement of knowledge about sludge drying, including the impact of the mechanical dewatering step. Before studying experimentally the influence of polymers type and dosage use for dewatering on the rheological properties and the drying process, it is necessary to assess the stability of the sludge during of the storage. Because sludge is a living material that can rapidly change, we use the method of experimental design in order to get maximum information with minimal testing, regarding sludge stability during storage. [less ▲]

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