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See detailFibronectin presence in native collagen fibrils of human fibroblasts: immunoperoxidase and immunoferritin localization.
Furcht, L. T.; Smith, D.; Wendelschafer-Crabb, G. et al

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1980), 28(12), 1319-33

Fibronectin is a major constituent of the fibroblast extracellular matrix. Fibronectin binds to collagen, mediates fibroblast adhesion to collagen, and is synthesized and secreted into the medium of ... [more ▼]

Fibronectin is a major constituent of the fibroblast extracellular matrix. Fibronectin binds to collagen, mediates fibroblast adhesion to collagen, and is synthesized and secreted into the medium of cultured fibroblasts. Affinity-purified antibodies to fibronectin and collagen were localized using the peroxidase-antiperoxidase method or with ferritin-coupled secondary antibodies. Using human fibroblasts cultured under routine conditions, fibronectin and procollagen I react in a nonperiodic manner with: 1) approximately 10 nm extracellular fibrils, 2) cell membrane, and 3) membrane-associated vesicles. All fibrils react with both antibodies, suggesting some form of codistribution of fibronectin and collagen in these fibrils. Treatment with ascorbate leads to the development of a larger diameter extracellular fibril, approximately 40 nm in diameter. These large diameter fibrils are clearly collagen fibrils as documented by the procollagen antibody reaction. Importantly, fibronectin is bound to or a constituent of these "native" or cellular made collagen fibrils. Fibronectin and procollagen antibodies localized with the peroxidase-antiperoxidase method have a 70 nm axial repeat of reaction product on ascorbate-treated fibroblasts. Localization of antibodies with ferritin-labeled secondary antibodies is less satisfactory, but supports the basic observations made with the unlabeled antibody enzyme method. This observation rules out any potential criticisms. Although it is more difficult to observe with immunoferritin, there is an indication that antibodies to fibronectin react with an axial periodicity on cellular produced collagen fibrils. [less ▲]

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See detailFibronectin Promotes Lung Colony Formation in the Mouse by B16 Melanoma Cells Spheroids
Coucke, P.; De Pauw-Gillet, Marie-Claire ULg; de Leval, Laurence ULg et al

in In Vivo (Athens, Greece) (1992), 6(5, Sep-Oct), 481-6

By microscopical observation and using an original method of automatic image analysis, we studied on histological sections the rate of lung colony formation after intravenous injection into the mouse of ... [more ▼]

By microscopical observation and using an original method of automatic image analysis, we studied on histological sections the rate of lung colony formation after intravenous injection into the mouse of B16 melanoma cells previously cultivated in vitro as pure or mixed spheroids (B16 + 3T3 fibroblasts). The preincubation in vitro of pure spheroids with fibronectin significantly increased the percentages of lung section area occupied by tumors and the relative number of internal lung colonies. This effect of fibronectin was even more obvious when mixed spheroids were injected. [less ▲]

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See detailFibronectin promotes rat Schwann cell growth and motility
Baron-Van Evercooren, Anne; Kleinman, Hinda K.; Seppa, H. E. et al

in Journal of Cell Biology (1982), 93(1), 211-216

Techniques are now available for culturing well characterized and purified Schwann cells. Therefore, we investigated the role of fibronectin in the adhesion, growth, and migration of cultured rat Schwann ... [more ▼]

Techniques are now available for culturing well characterized and purified Schwann cells. Therefore, we investigated the role of fibronectin in the adhesion, growth, and migration of cultured rat Schwann cells. Double-immunolabeling shows that, in primary cultures of rat sciatic nerve, Schwann cells (90%) rarely express fibronectin, whereas fibroblasts (10%) exhibit a granular cytoplasmic and fibrillar surface-associated fibronectin. Secondary cultures of purified Schwann cells do not express fibronectin. Exogenous fibronectin has a small effect on promoting the adhesion of Schwann cells to the substrate and does not significantly affect cell morphology, but it produced a surface fibrillar network on fibronectin on the secondary Schwann cells. Tritiated thymidine autoradiography revealed that addition of fibronectin to the medium, even at low concentrations, markedly stimulates Schwann cell proliferation, in both primary and secondary cultures. In addition, when cell migration was measured in a Boyden chamber assay, fibronectin was found to moderately, but clearly, stimulate directed migration or chemotaxis. [less ▲]

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See detailFibronectin: Adjunctive therapy in sepsis
Damas, Pierre ULg; Mahieu, P.; Adam, Aurore ULg

in Update in Intensive Care and Emergency Medicine (1987)

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See detailLa fibronectine
Lamy, Maurice ULg

in Presse Médicale (1983), 12(4), 211-212

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See detailLe Fibroscan: une nouvelle méthode d’évaluation non invasive de la fibrose hépatique
Lamproye, Anne ULg; Belaiche, Jacques ULg; Delwaide, Jean ULg

in Revue Médicale de Liège (2007), 62 Spec No

The FibroScan is a device allowing a non invasive diagnosis and quantification of liver fibrosis. The procedure is based on transient elastography and allows to record liver stiffness by measuring the ... [more ▼]

The FibroScan is a device allowing a non invasive diagnosis and quantification of liver fibrosis. The procedure is based on transient elastography and allows to record liver stiffness by measuring the velocity of shear wave across liver parenchyma. The elasticity is directly correlated to velocity of the wave. In chronic hepatitis C, there is a good correlation between liver elasticity and stage of fibrosis. The FibroScan has also been studied in other chronic liver diseases, such as hepatitis B, primary biliary cirrhosis, sclerosing cholangitis, auto-immune hepatitis, alcohol, steatosis, hemachromatosis with reproductible results. In a cirrhotic patient, it also allows to assess the severity of cirrhosis and to evaluate the risk of complication. It is a painless procedure, with a good acceptability by the patients. Therefore, the FibroScan can be regularly performed, allowing the follow up of fibrosis evolution over time. [less ▲]

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See detailFibrose mediastinale idiopathique.
Warling, O.; GUIOT, Julien ULg; Ramaut, M. et al

in Revue médicale de Liège (2013), 68(7-8), 408-11

Fibrosing mediastinitis is a rare condition characterized by an excessive growth of dense fibrous tissue within the mediastinum. The etiology of the disease is most often a fungal infection and may in ... [more ▼]

Fibrosing mediastinitis is a rare condition characterized by an excessive growth of dense fibrous tissue within the mediastinum. The etiology of the disease is most often a fungal infection and may in some cases be idiopathic. We present the case of a patient with chronic obstructive pulmonary disease (COPD) suffering from fibrosing mediastinitis of undetermined origin and in whom the diagnosis was established by histopathological analysis after mediastinoscopy. [less ▲]

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See detailLes fibroses pulmonaires idiopathiques: nouvelle classification histopathologique et aspects cliniques
Blaise, Pierre ULg; Louis, Renaud ULg; Bartsch, Pierre ULg

in Revue Médicale de Liège (2000), 55(11), 986-90

Idiopathic pulmonary fibrosis (IPF) comprises different interstitial lung diseases of unknown origin. They are classified into four groups based on pathologic criteria: UIP, DIP, AIP and NSIP. This ... [more ▼]

Idiopathic pulmonary fibrosis (IPF) comprises different interstitial lung diseases of unknown origin. They are classified into four groups based on pathologic criteria: UIP, DIP, AIP and NSIP. This classification helps in terms of prognosis and treatment. In addition to history, physical examination and common investigations (chest X ray, pulmonary function testing), a bronchoalveolar lavage (BAL), a high-resolution CT scan and ideally a open-lung biopsy are needed to establish a precise diagnosis. The patients are usually treated by immunosuppressive agents, and mainly by corticoids. However, in UIP, lung transplantation is the only way to improve survival. [less ▲]

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See detailFibrosis and pulmonary function tests - New therapeutic options for respiratory diseases.
Clercx, Cécile ULg

in Proceedings of the Meeting of German specialists in internal medicine (ECVIM-CA-Internal Medicine) - Viernheim - 3rd December 2011 (2011, December)

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See detailFibrous mucosa preservation with NobelGuide
Bolland, Fabrice; Van Heusden, Alain ULg

Poster (2007)

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See detailFibrous natural materials for ceramic processing
Gaydardzhiev, Stoyan ULg; Wilker, Viola; Scheffler, Michael

in Zuo, Wang (Ed.) Proceedings of the XXIV International Mineral Processing Congress (2008, September)

Initial experiments towards preparation of hollow ceramic microtubes by dip-coating of fibrous plants with water based alumina gel-casting slurries using hemp and coconut fibres as templates have been ... [more ▼]

Initial experiments towards preparation of hollow ceramic microtubes by dip-coating of fibrous plants with water based alumina gel-casting slurries using hemp and coconut fibres as templates have been done. It has been found that the wettability of fibres and accordingly the stability of the developed ceramic layer on their surface during slurry gelation varied significantly within the different fibre types. Good correlation between the diameters of the template fibres and the resulting channels has been found at the 31 % solids loadings level. Hemp fibres used as bundles have led to formation of multi channel patterns while the coconut fibres-templated process resulted in uniform monochannel materials. The micro tubes have shown good mechanical handling properties. [less ▲]

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See detailFibulin 3 peptides Fib3-1 and Fib3-2 are potential biomarkers of osteoarthritis.
Henrotin, Yves ULg; Gharbi, Myriam; Mazzucchelli, Gabriel ULg et al

in Arthritis and Rheumatism (2012), 64(7), 2260-7

OBJECTIVE: This study was undertaken to identify new biomarkers of osteoarthritis (OA) by proteomics analysis and to develop specific immunoassays to detect and quantify them. METHODS: Proteomics analysis ... [more ▼]

OBJECTIVE: This study was undertaken to identify new biomarkers of osteoarthritis (OA) by proteomics analysis and to develop specific immunoassays to detect and quantify them. METHODS: Proteomics analysis was performed in urine samples from 10 women (mean+/-SD age 76.0+/-5.0 years) undergoing knee replacement surgery due to severe OA and 5 healthy women (mean+/-SD age 25.6+/-2.6 years). Protein content was analyzed by 2-dimensional differential gel electrophoresis. Protein spots that exhibited an OA:control abundance ratio of >/=1.5 were identified by mass spectrometry. Specific enzyme-linked immunosorbent assays were developed and validated in serum obtained from 236 healthy subjects ages 20-64 years and from 76 patients with severe radiologic knee OA (mean+/-SD age 68.8+/-11.9 years). Immunohistochemical analysis was performed on articular cartilage from tibial plateaus. RESULTS: Thirteen proteins within spots that were significantly modified between groups were identified. Two peptides of fibulin 3, named Fib3-1 and Fib3-2, were of particular interest. Two antisera directed against these peptides were used to develop immunoassays. Compared with age-matched healthy subjects, median levels of serum Fib3-1 and Fib3-2 were elevated in OA patients (54.6 pM versus 85.1 pM [P<0.0001] and 144.4 pM versus 191.4 pM [P<0.0001], respectively). Using area under the receiver operating characteristic curve analysis, we demonstrated that Fib3-1 and Fib3-2 levels discriminate between OA and normal populations. Immunostaining revealed the presence of Fib3-1 and Fib3-2 in chondrocytes and in the extracellular matrix of the superficial layer of the fibrillated cartilage. CONCLUSION: Our findings indicate that Fib3-1 and Fib3-2 are potential biochemical markers for the diagnosis of OA. [less ▲]

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See detailFibulin-3 fragments (FIB3-1 and FIB3-2) are potential new biomarkers for the diagnosis of osteoarthritis
Gharbi, M; Dubuc, JE; Deberg, Michelle ULg et al

in Annals of the Rheumatic Diseases (2011), 70(Suppl 3), 354

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See detailFICEB W.P.6 final report: Numerical simulations of the large scale fire test
Gernay, Thomas ULg; Franssen, Jean-Marc ULg

Report (2010)

This report presents the results of the numerical simulations performed by University of Liege in the framework of work package 6 of the FICEB project financed by the Research Fund for Coal and Steel. A ... [more ▼]

This report presents the results of the numerical simulations performed by University of Liege in the framework of work package 6 of the FICEB project financed by the Research Fund for Coal and Steel. A full scale fire test was performed in Ulster on February 27th 2010 on a composite floor for analysing the behaviour of long cellular steel beams in a complete compartment office in building structure under realistic fire conditions. The aim was to study the possibility of tensile membrane action to develop when the unprotected steel beams in the central part of the floor are made of cellular beams. Predictive numerical simulations of the test have been performed with the software SAFIR in order to define the appropriate structure to be used for the full scale fire test. As predicted, the structure was able to withstand the test without collapse. After the test, the results of the predictive simulations have been compared to the measured results, showing relatively good agreement. [less ▲]

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See detailFiche descriptive - Erable jaspé de gris (Acer rufinerve)
Halford, Mathieu ULg; Frisson, Gwenn ULg; Delbart, Emmanuel ULg et al

Learning material (2010)

Detailed reference viewed: 30 (3 ULg)