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See detailGrowth, fluorescence, photosynthetic O-2 production and pigment content of salt adapted cultures of Arthrospira (Spirulina) platensis
Ben Dhiab, R.; Ben Ouada, H.; Boussetta, H. et al

in Journal of Applied Phycology (2007), 19(4), 293-301

The effect of salt concentration (NaCl) on growth, fluorescence, photosynthetic activities and pigment content of the cyanobacterium Arthrospira platensis has been investigated over 15 days. It has been ... [more ▼]

The effect of salt concentration (NaCl) on growth, fluorescence, photosynthetic activities and pigment content of the cyanobacterium Arthrospira platensis has been investigated over 15 days. It has been observed that high NaCl concentration induces an increase of the growth, photosynthetic efficiency (alpha), phycobilin/chlorophyll ratio and a slight decrease of dark respiration and compensation points. Moreover, high NaCl concentration enhances photosystem II (PSII) activity compared to photosystem I (PSI). Results show that the phycobilin-PSII energy transfer compared to the chlorophyll-PSII (F-695,F-600/F-695,F-440) increases. However, data obtained about the maximal efficiency of PSII photochemistry are controversial. Indeed, the Fv/Fm ratio decreases in salt adapted cultures, while at the same time the trapping flux per PSII reaction center (TR0/RC) and the probability of electron transport beyond QA (Psi(0)) remain unchanged at the level of the donor and the acceptor sites of PSII. This effect can be attributed to the interference of phycobilin fluorescence with Chl a when performing polyphasic transient measurements. [less ▲]

Detailed reference viewed: 115 (3 ULg)
See detailGrowth, milk and meat production with a Belgian Blue x Holstein F1 herd
Gielen, Marc ULg; Dufrasne, Isabelle ULg; Hollo, Véronique et al

in Proceedings of the 42nd Annual Meeting of E.A.A.P. (1991)

Detailed reference viewed: 3 (1 ULg)
See detailGrowth-hormone binding protein (GHBP) binds prolactin in cattle plasma
Massart, Serge; Ban, Anne-Marie; Renaville, Robert ULg et al

Conference (1995)

Detailed reference viewed: 19 (2 ULg)
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See detailGrowth-induced haemodynamic changes in healthy Friesian calves
Amory, Hélène ULg; Desmecht, Daniel ULg; Linden, Annick ULg et al

in Veterinary Record : Journal of the British Veterinary Association (1993), 132(17), 426-434

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See detailGrowth-inhibiting effects of a neem-based insecticide (Margosan-O) against Spodoptera littoralis (Lepidoptera: Noctuidae
Haubruge, Eric ULg; Seck, Dogo; Hemptinne, Jean-Louis et al

in Insect Science and its application (1994), 15(4), 243-249

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See detailGrowth-modulating molecules are associated with invading Schwann cells and not astrocytes in human traumatic spinal cord injury
Buss, A.; Pech, K.; Kakulas, B. A. et al

in Brain (2007), 130(Part 4), 940-953

Despite considerable progress in recent years, the underlying mechanisms responsible for the failure of axonal regeneration after spinal cord injury (SCI) remain only partially understood. Experimental ... [more ▼]

Despite considerable progress in recent years, the underlying mechanisms responsible for the failure of axonal regeneration after spinal cord injury (SCI) remain only partially understood. Experimental data have demonstrated that a major impediment to the outgrowth of severed axons is the scar tissue that finally dominates the lesion site and, in severe injuries, is comprised of connective tissue and fluid-filled cysts, surrounded by a dense astroglial scar. Reactive astrocytes and infiltrating cells, such as fibroblasts, produce a dense extracellular matrix (ECM) that represents a physical and molecular barrier to axon regeneration. In the human situation, correlative data on the molecular composition of the scar tissue that forms following traumatic SCI is scarce. A detailed investigation on the expression of putative growth-inhibitory and growth-promoting molecules was therefore performed in samples of post-mortem human spinal cord, taken from patients who died following severe traumatic SCI. The lesion-induced scar could be subdivided into a Schwann cell dominated domain which contained large neuromas and a surrounding dense ECM, and a well delineated astroglial scar that isolated the Schwann cell/ECM rich territories from the intact spinal parenchyma. The axon growth-modulating molecules collagen IV, laminin and fibronectin were all present in the post-traumatic scar tissue. These molecules were almost exclusively found in the Schwann cell-rich domain which had an apparent growth-promoting effect on PNS axons. In the astrocytic domain, these molecules were restricted to blood vessel walls without a co-localization with the few regenerating CNS neurites located in this region. Taken together, these results favour the notion that it is the astroglial compartment that plays a dominant role in preventing CNS axon regeneration. The failure to demonstrate any collagen IV, laminin or fibronectin upregulation associated with the astroglial scar suggests that other molecules may play a more significant role in preventing axon regeneration following human SCI. [less ▲]

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See detailGrowth-Related Changes in the Pulmonary Function of Goats
Bakima, M.; Lomba, Fernand; Lekeux, Pierre ULg

in Veterinary Research Communications (1990), 14(2), 141-146

Growth-related changes in pulmonary function values were investigated in 20 healthy French Alpine goats, aged between 20 and 550 days, weighing 7-55 kg. Pulmonary ventilation, mechanics of breathing and ... [more ▼]

Growth-related changes in pulmonary function values were investigated in 20 healthy French Alpine goats, aged between 20 and 550 days, weighing 7-55 kg. Pulmonary ventilation, mechanics of breathing and arterial oxygen tension were measured using standardized techniques and methods adapted for goats of different body sizes. The Ppl values and the tI/tTOT ratio showed no significant changes with age and body size. The ventilation values (Vt, Ve, mVI and mVE) increased linearly with growth. There was a significant correlation of age and body weight with dynamic lung compliance (Cdyn), total pulmonary resistance (RL), viscous work of breathing (Wvis tot) and minute viscous work (Wvis min) throughout the age range studied. Cdyn, Wvis tot and Wvis min increased and RL decreased with age and body weight. Arterial blood gases (PaO2 and PaCO2) did not show significant changes over the age range studied. Regression equations for each pulmonary function parameter are given with body weight as the independent variable. Data for the mechanics of breathing were compared with those elsewhere for cattle, horses, man and dogs. [less ▲]

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See detailGrupo de Trabajo para el manejo del infarto agudo de miocardio con elevacion del segmento ST de la Sociedad Europea de Cardiologia (ESC).
Steg, Gabriel; James, Stefan K.; Atar, Dan et al

in Revista Espanola de Cardiologia (2013), 66(1), 531-5346

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See detailGrupurile informale : primii pasi in actiunea colectiva
Teclu, Codrat Alin ULg

in Lambru, Mihaela; Vamescu, Ancuta; Kivu, Mircea (Eds.) Romania 2010 : Sectorul neguvernamental - profil, tendinte, provocari (2010)

L'ouvrage se propose de dresser un portrait quantitatif et qualitatif du secteur non-gouvernemental roumain 10 ans après la dernière grande enquête sur le sujet. Les principaux défis des ONG sont ... [more ▼]

L'ouvrage se propose de dresser un portrait quantitatif et qualitatif du secteur non-gouvernemental roumain 10 ans après la dernière grande enquête sur le sujet. Les principaux défis des ONG sont également abordés. Notre contribution sur les groupes informels de citoyens se trouve en pp. 90-93. [less ▲]

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See detailGs alpha overexpression and loss of Gs alpha imprinting in human somatotroph adenomas: association with tumor size and response to pharmacologic treatment.
Picard, Christophe; Silvy, Monique; Gerard, c et al

in International Journal of Cancer = Journal International du Cancer (2007), 121(6), 1245-1252

Gs alpha, the alpha-subunit of the heterotrimeric GTP-binding protein, is coded from the GNAS gene, which is imprinted in a tissue-specific manner. Gs alpha is paternally silenced in normal pituitary, but ... [more ▼]

Gs alpha, the alpha-subunit of the heterotrimeric GTP-binding protein, is coded from the GNAS gene, which is imprinted in a tissue-specific manner. Gs alpha is paternally silenced in normal pituitary, but Gs alpha imprinting relaxation is found in some tumoral tissue. In addition, Gs alpha mRNA levels are high in some somatotroph adenomas not bearing the active Gs alpha mutant, the gsp oncogene. In this study, the impact of loss of imprinting on Gs alpha expression level and on tumoral phenotype has been investigated. We compared the expression and imprinting of 4 transcripts of GNAS locus (NESP55, XL alpha s, exon 1A, Gs alpha) of 60 somatotroph adenomas with those of 23 lactotroph adenomas. The paternal and maternal transcripts were quantified using allele-specific real-time PCR and FokI polymorphism. Moreover, the methylation of exon 1A DMR was analyzed. As is the case for the gsp oncogene, high Gs alpha expression in gsp- tumors was associated with smaller tumor size and better octreotide sensitivity. A strong imprinting relaxation (percentage of paternal Gs alpha expression >or=7.5%) was found only in gsp- tumors. The loss of Gs alpha imprinting was associated with a decrease in exon 1A mRNA expression. Unexpectedly, the methylation status of exon 1A DMR was not modified in relaxed tumors. Maternal Gs alpha mRNA level decreased with exon 1A level, and consequently the loss of Gs alpha imprinting did not induce the expected Gs alpha overexpression. Finally, XL alpha s mRNA level correlated with that of paternal Gs alpha and of NESP55 showing the complexity of gene regulation in the GNAS locus. [less ▲]

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See detailGSK-3-specific inhibitor-supplemented hESC medium prevents the epithelial-mesenchymal transition process and the up-regulation of matrix metalloproteinases in hESCs cultured in feeder-free conditions
Ullmann, U.; Gilles, Christine ULg; De Rycke, M. et al

in Molecular Human Reproduction (2008), 14(3), 169-79

Feeder-free culture induces spontaneous differentiation of human embryonic stem cells (hESCs), identified as an epithelial to mesenchymal transition (EMT). The maintenance of pluripotency of hESCs in ... [more ▼]

Feeder-free culture induces spontaneous differentiation of human embryonic stem cells (hESCs), identified as an epithelial to mesenchymal transition (EMT). The maintenance of pluripotency of hESCs in feeder-free cultures through the activation of the WNT pathway using a glycogen synthase kinase (GSK)-3-specific inhibitor (BIO) was reported. The aim of this study was to determine the effect of BIO on the EMT process. In contrast with those grown in feeder-free conditions with control medium, hESC colonies cultured with BIO-supplemented hESC medium did not show any fibroblast-like cells at the periphery. Transmission electron microscopy, relative quantitative real-time RT–PCR and immunostaining analyses showed the presence of epithelial features and a diminution of mesenchymal features in the BIO-treated hESCs such as a strong E-cadherin expression, the down-regulation of Vimentin, Snail and Slug expressions and a cytoplasmic β-catenin expression. An up-regulation of matrix metalloproteinases (MMP) MMP-2, MMP-9, MT-1MMP (membrane-type 1 MMP) and EMMPRIN (extracellular MMP inducer) expression was also found associated with the EMT occurring in feeder-free hESCs cultures using mouse embryonic fibroblasts conditioned medium (MEF CM). The presence of BIO clearly down-regulated the expression of these MMPs. This study showed that BIO, a GSK-3-specific inhibitor, prevents the EMT process which is associated with the feeder-free hESC culture. Nevertheless, BIO was not sufficient to expand hESCs in a long-term culture system. [less ▲]

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See detailGSK3-Mediated BCL-3 phosphorylation modulates its degradation and its oncogenicity
Viatour, Patrick ULg; Dejardin, Emmanuel ULg; Warnier, Michael et al

in Molecular Cell (2004), 16(1), 35-45

The oncoprotein BCL-3 is a nuclear transcription factor that activates NF-kappaB target genes through formation of heterocomplexes with p50 or p52. BCL-3 is phosphorylated in vivo, but specific BCL-3 ... [more ▼]

The oncoprotein BCL-3 is a nuclear transcription factor that activates NF-kappaB target genes through formation of heterocomplexes with p50 or p52. BCL-3 is phosphorylated in vivo, but specific BCL-3 kinases have not been identified so far. In this report, we show that BCL-3 is a substrate for the protein kinase GSK3 and that GSK3-mediated BCL-3 phosphorylation, which is inhibited by Akt activation, targets its degradation through the proteasome pathway. This phosphorylation modulates its association with HDAC1, -3, and -6 and attenuates its oncogenicity by selectively controlling the expression of a subset of newly identified target genes such as SLPI and CxcI1. Our results therefore suggest that constitutive BCL-3 phosphorylation by GSK3 regulates BCL-3 turnover and transcriptional activity. [less ▲]

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See detailLe GSM domine Internet
Hermans, Michel ULg

Article for general public (2007)

Detailed reference viewed: 15 (3 ULg)
See detailGTI : Gestion de Thesaurus interactive, logiciel pour PC
Coibion, Claude ULg

in Cahiers de la Documentation = Bladen voor Documentatie (1987), 3

GTI est un logiciel de gestion, maintenance et exploitation interactive de thésaurus sur micro-ordinateur IBM PC et compatibles. Il contrôle la création et la gestion des relations sémantiques des ... [more ▼]

GTI est un logiciel de gestion, maintenance et exploitation interactive de thésaurus sur micro-ordinateur IBM PC et compatibles. Il contrôle la création et la gestion des relations sémantiques des descripteurs et non-descripteurs, ainsi que des champs sémantiques qui leur sont associés. Le thésaurus ainsi construit peut être consulté en ligne par affichage des relations triées d'un descripteur particulier, ou en imprimant la liste alphabétique structurée complète des termes. [less ▲]

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See detailGuanidinium chloride denaturation of the dimeric Bacillus licheniformis Blal repressor highlights an independent domain unfolding pathway
Vreuls, Christelle ULg; Filée, Patrice ULg; Van Melckebeke, H. et al

in Biochemical Journal (2004), 384(Pt 1), 179-190

The Bacillus licheniformis 74911 BlaI repressor is a prokaryotic regulator that, in the absence of a P-lactam antibiotic, prevents the transcription of the blaP gene, which encodes the BlaP beta-lactamase ... [more ▼]

The Bacillus licheniformis 74911 BlaI repressor is a prokaryotic regulator that, in the absence of a P-lactam antibiotic, prevents the transcription of the blaP gene, which encodes the BlaP beta-lactamase. The BlaI repressor is composed of two structural domains. The 82-residue NTD (N-terminal domain) is a DNA-binding domain, and the CTD (C-terminal domain) containing the next 46 residues is a dimerization domain. Recent studies have shown the existence of the monomeric, dimeric and tetrameric forms of BlaI in solution. In the present study, we analyse the equilibrium unfolding of BlaI in the presence of GdmCl (guanidinium chloride) using different techniques: intrinsic and ANS (8-anilinonaphthalene-1-sulphonic acid) fluorescence, far- and near-UV CD spectroscopy, cross-linking, analytical ultracentrifugation, size exclusion chromatography and NMR spectroscopy. In addition, the intact NTD and CTD were purified after proteolysis of BlaI by papain, and their unfolding by GdmCl was also studied. GdmCl-induced equilibrium unfolding was shown to be fully reversible for BlaI and for the two isolated fragments. The results demonstrate that the NTD and CTD of BlaI fold/unfold independently in a four-step process, with no significant cooperative interactions between them. During the first step, the unfolding of the Blal CTD occurs, followed in the second step by the formation of an 'ANS-bound' intermediate state. Crosslinking and analytical ultracentrifugation experiments suggest that the dissociation of the dimer into two partially unfolded monomers takes place in the third step. Finally, the unfolding of the Blal NTD occurs at a GdmCI concentration of approx. 4 M. In summary, it is shown that the Blal CTD is structured, more flexible and less stable than the NTD upon GdmCI denaturation. These results contribute to the characterization of the Blal dimerization domain (i.e. CTD) involved in the induction process. [less ▲]

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See detailGuanines are a quartet's best friend: impact of base substitutions on the kinetics and stability of tetramolecular quadruplexes
Gros, Julien; Rosu, Frédéric ULg; Amrane, Samir et al

in Nucleic Acids Research (2007), 35(9), 3064-3075

Parallel tetramolecular quadruplexes may be formed with short oligodeoxynucleotides bearing a block of three or more guanines. We analyze the properties of sequence variants of parallel quadruplexes in ... [more ▼]

Parallel tetramolecular quadruplexes may be formed with short oligodeoxynucleotides bearing a block of three or more guanines. We analyze the properties of sequence variants of parallel quadruplexes in which each guanine of the central block was systematically substituted with a different base. Twelve types of substitutions were assessed in more than 100 different sequences. We conducted a comparative kinetic analysis of all tetramers. Electrospray mass spectrometry was used to count the number of inner cations, which is an indicator of the number of effective tetrads. In general, the presence of a single substitution has a strong deleterious impact on quadruplex stability, resulting in reduced quadruplex lifetime/ thermal stability and in decreased association rate constants. We demonstrate extremely large differences in the association rate constants of these quadruplexes depending on modification position and type. These results demonstrate that most guanine substitutions are deleterious to tetramolecular quadruplex structure. Despite the presence of well- defined non- guanine base quartets in a number of NMR and X- ray structures, our data suggest that most non- guanine quartets do not participate favorably in structural stability, and that these quartets are formed only by virtue of the docking platform provided by neighboring G- quartets. Two notable exceptions were found with 8- bromoguanine ( X) and 6- methyl- isoxanthopterin ( P) substitutions, which accelerate quadruplex formation by a factor of 10 when present at the 50 end. The thermodynamic and kinetic data compiled here are highly valuable for the design of DNA quadruplex assemblies with tunable association/ dissociation properties. [less ▲]

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See detailGuatemala desarrolla diagnóstico nacional de riego
Estrada Orozco, Nick Kenner ULg

Article for general public (2012)

El diagnóstico del riego en Guatemala, tiene como propósito proporcionar la información antecedente para el diseño de una política nacional de riego, que se proyecte en el corto, mediano y largo plazo ... [more ▼]

El diagnóstico del riego en Guatemala, tiene como propósito proporcionar la información antecedente para el diseño de una política nacional de riego, que se proyecte en el corto, mediano y largo plazo. Para este proceso, se han unido los esfuerzos del Ministerio de Agricultura Ganaderia y Alimentación (MAGA), y la organización de las Naciones Unidas para la Alimentación y la Agricultura a través de programa conjunto de las naciones unidas “Fortaleciendo las capacidades con el pueblo Mam para la Gobernabilidad económica en Agua y saneamiento”. [less ▲]

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See detailLa guattegaumerine, nouvel alcaloide bisbenzylisoquinoleinique de Guatteria gaumeri
Dehaussy, H.; Tits, Monique ULg; Angenot, Luc ULg

in Planta Medica (1983), 49(9), 25-7

The aqueous-alcoholic extract of the bark of GUATTERIA GAUMERI (Mexican Annonaceae) is used for the treatment of cholelithiasis and hypercholesterolemia. Therefore it seems very interesting to perform a ... [more ▼]

The aqueous-alcoholic extract of the bark of GUATTERIA GAUMERI (Mexican Annonaceae) is used for the treatment of cholelithiasis and hypercholesterolemia. Therefore it seems very interesting to perform a phytochemical study of this drug in order to know its composition and to determine, if possible, all the active principles. We describe now the isolation of the main alkaloid named guattegaumerine. This new bisbenzylisoquinoline alkaloid has been assigned structure 1, on the basis of spectroscopic, chemical and chromatographic evidence. [less ▲]

Detailed reference viewed: 41 (2 ULg)