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See detailExpression and Induction of Drug-Metabolizing Enzymes in Cultured Fetal Rat Hepatocytes
Kremers, Pierre ULg; Roelandt, L.; Stouvenakers, Nadine ULg et al

in Cell Biology and Toxicology (1994), 10(2), 117-25

An in vitro experimental model, fetal rat hepatocytes in culture, was metabolically characterized. Several enzymatic activities were expressed in these hepatocytes, namely, testosterone hydroxylations ... [more ▼]

An in vitro experimental model, fetal rat hepatocytes in culture, was metabolically characterized. Several enzymatic activities were expressed in these hepatocytes, namely, testosterone hydroxylations. Hepatocytes cultured up to 3 weeks in the presence of dexamethasone and phenobarbital still expressed some drug-metabolizing enzyme activities (e.g., ECOD). The enzymatic activities were measured both directly on monolayers during culture and on the corresponding harvested and homogenized cells. The results correlate perfectly with each other. The 'on cell' procedure allows us to repeat the assay or to measure several activities on the same cells at different time intervals. The presence of dexamethasone in the culture medium allows the expression and the induction of several cytochrome P450 isoenzymes, namely, those hydroxylating testosterone. This makes the model particularly attractive for induction experiments as well as for metabolic or toxicological studies needing longer treatments. [less ▲]

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See detailExpression and Modulation of Homeobox Genes from Cluster B in Endothelial Cells
Belotti, D.; Clausse, Nathalie; Flagiello, D. et al

in Laboratory Investigation : Journal of Technical Methods & Pathology (1998), 78(10), 1291-9

Angiogenesis is a complex phenomenon likely to be under the strict control of a group of transcription factor(s). Homeobox (HOX)-containing proteins have been identified as regulators controlling the ... [more ▼]

Angiogenesis is a complex phenomenon likely to be under the strict control of a group of transcription factor(s). Homeobox (HOX)-containing proteins have been identified as regulators controlling the coordinated expression of genes involved in organ development and tissue differentiation. In this study, we have demonstrated that human umbilical vein endothelial cells (HUVEC) express 8 of the 10 HOX genes contained in cluster B. Treatment of HUVEC with tissue plasminogen activator (TPA), an agent known to induce morphologic changes in endothelial cells, or vascular endothelium growth factor (VEGF), a proliferative and angiogenesis inducer, results in a specific time-dependent modulation of the eight HOX genes identified. Interestingly, neither basic fibroblast growth factor, an endothelial proliferative agent, nor TNP-470, a fumagillin derivative with potent antiendothelial cell proliferation properties, affected expression of these HOX genes. Specific modulation of HOX genes by differentiating agents but not by proliferative or antiproliferative molecules suggests that they could be involved in the control of the genetic program that coordinates the construction of new blood vessels. [less ▲]

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See detailExpression and nuclear location of the transcriptional repressor kaiso is regulated by the tumor microenvironment
Soubry, Adelheid; van Hengel, Jolanda; Parthoens, Eef et al

in Cancer Research (2005), 65(6), 2224-2233

Kaiso is a BTB/POZ zinc finger protein originally described as an interaction partner of p120ctn. In cultured cell lines, Kaiso is found almost exclusively in the nucleus, where it generally acts as a ... [more ▼]

Kaiso is a BTB/POZ zinc finger protein originally described as an interaction partner of p120ctn. In cultured cell lines, Kaiso is found almost exclusively in the nucleus, where it generally acts as a transcriptional repressor. Here, we describe the first in situ immunolocalization studies of Kaiso expression in normal and cancerous tissues. Surprisingly, we found striking differences between its behavior in monolayers of different cell lines, three-dimensional cell culture systems, and in vivo. Although nuclear localization was sometimes observed in tissues, Kaiso was more often found in the cytoplasm, and in some cell types it was absent. In general, Kaiso and p120ctn did not colocalize in the nucleus. To examine this phenomenon more carefully, tumor cells exhibiting strong nuclear Kaiso staining in vitro were injected into nude mice and grown as xenografts. The latter showed a progressive translocation of Kaiso towards the cytoplasm over time, and even complete loss of expression, especially in the center of the tumor nodules. When xenografted tumors were returned to cell culture, Kaiso was re-expressed and was once again found in the nucleus. Translocation of Kaiso to the cytoplasm and down-regulation of its levels were also observed under particular experimental conditions in vitro, such as formation of spheroids and acini. These data strongly imply an unexpected influence of the microenvironment on Kaiso expression and localization. As transcriptional repression is a nuclear event, this phenomenon is likely a crucial factor in the regulation of Kaiso function. [less ▲]

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See detailExpression and purification of the human Tre2 product, a putative Ypt/Rab GTPase activating protein.
Bizimungu, Christelle; Stroobants, Aurore ULg; Tricot, Catherine et al

in Archives Internationales de Physiologie et de Biochimie (2005), 191

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See detailExpression and Secretion of the Human Placental Growth Hormone in Escherichia Coli
Igout, Ahmed ULg; Scippo, Marie-Louise ULg; Frankenne, Francis ULg et al

in Nucleic Acids Research (1989), 17(10), 3998

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See detailExpression At The Cell-Surface Of Native Fusion Protein Of The Newcastle-Disease Virus (Ndv) Strain Italien From Cloned Cdna
Espion, D.; Dehenau, S.; Letellier, C. et al

in Archives of Virology (1987), 95(1-2), 79-95

A cDNA library was constructed with poly(A)+-mRNAs from NDV-Italien infected BHK-21 cells. A clone, that hybridized to the F gene mRNA, was sequenced. A long open reading frame encodes for a protein of ... [more ▼]

A cDNA library was constructed with poly(A)+-mRNAs from NDV-Italien infected BHK-21 cells. A clone, that hybridized to the F gene mRNA, was sequenced. A long open reading frame encodes for a protein of 553 amino acids, with a calculated molecular weight of 59,153, consisting of twelve cysteine residues and six potential glycosylation sites. The protein sequence contains a hydrophobic region at the N-terminus of F1 and a presumptive long transmembrane fragment near the C-terminus. Comparison of the F proteins from NDV strains Italien and Australia-Victoria shows that the sequences are very similar, with conservation of most cysteine residues and of the potential glycosylation sites. The F coding sequence was inserted into the genome of vaccinia virus under the control of vaccinia P7.5 transcriptional regulatory sequences. Expression of F protein was demonstrated by indirect immunofluorescence with five anti-F monoclonal antibodies known to react with conformational epitopes. [less ▲]

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See detailExpression characteristics of potential biomarker genes in Tra catfish, Pangasianodon hypophthalmus, exposed to trichlorfon
Sinha, Amit Kumar; Vanparys, Caroline; De Boeck et al

in Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics (2010), 5(3), 207-216

Trichlorfon (TRC) is the most common organophosphorous insecticide used in aquaculture practices in Southeast Asian countries. Indiscriminate use of TRC can either damage or alter the enzymatic and ... [more ▼]

Trichlorfon (TRC) is the most common organophosphorous insecticide used in aquaculture practices in Southeast Asian countries. Indiscriminate use of TRC can either damage or alter the enzymatic and hormonal activities in the living organisms. In this present study, therefore, toxicogenomic analyses using real time PCR was used to characterize expression levels of various genes in Pangasianodon hypophthalmus after exposure to three concentrations, the 96 h 1/100LC50 (0.01 mg/L), the 96 h 1⁄10LC50 (0.1 mg/L) and the 96 h 1⁄2LC50 (0.5 mg/L) of TRC for 6 h, 24 h, 96 h, 7 days, 14 days, 28 days and 56 days respectively. The expression kinetics of stress and other cellular toxicity representative genes such as heat shock protein70 (HSP70), growth hormone, acetylcholinesterase (AChE), trypsinogen, cytochrome P4501B (CYP1B) and cytochrome oxidase subunit 1 (COI) were investigated in liver and gills. TRC at a level of 0.1 mg/L and 0.5 mg/L induced a time and dose-dependent increase in the expression of the HSP70, COI and CYPIB while the transcript level of AChE, growth hormone and trypsinogen were significantly down-regulated. These results could permit to develop a ‘molecular biomarker system’ which can be applied as a first-tier method of identifying contaminant exposure before effects at population level occur. [less ▲]

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See detailExpression complète des termes du second ordre dans les formules de réduction au lieu apparent
Folie, François ULg

in Annuaire de l'Observatoire Royal de Belgique (1896), 63

The author proves that several observations in right ascension provide better results if we take into account the refraction in the calculated declination.

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See detailExpression complète et signification véritable de la nutation initiale: démonstration qui en résulte de la fluidité intérieure du globe: conséquences analytiques de celle-ci dans les formules de l'astronomie
Folie, François ULg

in Acta Mathematica (1892), 16(1), 365-384

Astronomers neglected for a long time the nutation resulting from the arbitrary constants that the integration introduces into the Earth's rotation equations. This eulerian nutation, or initial nutation ... [more ▼]

Astronomers neglected for a long time the nutation resulting from the arbitrary constants that the integration introduces into the Earth's rotation equations. This eulerian nutation, or initial nutation, entered in the practical domain and the scientists didn't worry any more about its diurnal nature, even going as far as disputing it. However, it is obvious that this feater is the simplest and the safest to determine the constants of this nutation. [less ▲]

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See detailExpression dans le double mutant levurien msb3msb4 de 2 gènes humains homologues
Bizimungu, Christelle; Burny, Arsène; Portetelle, Daniel ULg et al

Poster (2003)

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See detailExpression dans le double mutant levurien msb3msb4 de 2 gènes humains homologues
Bizimungu, Christelle; Burny, Arsène; Portetelle, Daniel ULg et al

Poster (2003, April)

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See detailExpression de l'antigene DRC1 par les cellules leucemiques.
Antoine, Nadine ULg; Beckers, Catherine ULg; Marcoty, C. et al

in Revue Médicale de Liège (1992), 47(2), 95-9

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See detailL'expression de l'heure dans le système de l'axe instantané
Folie, François ULg

in Bulletins de l'Académie Royale des Sciences, des Lettres et des Beaux-Arts de Belgique (1897), 3e série, t. 33(5), 397-406

The author shows that the absolute uniformity of hour is the base of the spherical astronomy.

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See detailExpression des récepteurs somatostatinergiques SSTR2 et dopaminergiques D2 dans les adénomes somatotropes
Tabarin, A.; Ronci, N.; Carrié, F. et al

in Annales d'Endocrinologie (1997), 58(2),

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See detailL'expression du sujet nominal au Présent I en néo-égyptien
Winand, Jean ULg

in Chronique d'Egypte : Bulletin Périodique de la Fondation Egyptologique Reine Elisabeth (1989), 64

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