Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailFungal biofilm reactor improves the productivity of hydrophobin HFBII
Khalesi, Mohammadreza; Zune, Quentin ULg; Telek, Samuel ULg et al

in Biochemical Engineering Journal (in press)

Production and purification of hydrophobin HFBII has recently been the subject of intensive research, but the yield of production needs to be further improved for a generic use of this molecule at ... [more ▼]

Production and purification of hydrophobin HFBII has recently been the subject of intensive research, but the yield of production needs to be further improved for a generic use of this molecule at industrial scale. In a first step, the influence of different carbon sources on the growth of Trichoderma reesei and the production of HFBII was investigated. The optimum productivity was obtained by using 40 g/L lactose. Carbon starvation and excretion of extracellular enzyme were determined as two main conditions for the production of HFBII. In the second phase, and according to the physiological mechanisms observed during the screening phase, a bioreactor set up has been designed and two modes of cultures have been investigated, i.e. the classical submerged fermentation and a fungal biofilm reactor. In this last set-up, the broth is continuously recirculated on a metal packing exhibiting a high specific surface. In this case, the fungal biomass was mainly attached to the metal packing, leading to a simplification of downstream processing scheme. More importantly, the HFBII concentration increased up to 48.6 ± 6.2 mg/L which was 1.8 times higher in this reactor configuration and faster than the submerged culture. X-ray tomography analysis shows that the biofilm overgrowth occurs when successive cultures are performed on the same packing. However, this phenomenon has no significant influence on the yield of HFBII, suggesting that this process could be operated in continuous mode. Protein hydrolysis during stationary phase was observed by MALDI-TOF analysis according to the removal of the last amino acid from the structure of HFBII after 48 h from the beginning of fermentation in biofilm reactor. Hopefully this modification does not lead to alternation of the main physicochemical properties of HFBII. [less ▲]

Detailed reference viewed: 34 (19 ULg)
Full Text
Peer Reviewed
See detailFungal chitin-glucan scaffold for managing diabetic xerosis of the feet in menopausal women.
Quatresooz, Pascale ULg; Franchimont, Claudine ULg; Szepetiuk, Grégory ULg et al

in Expert Opinion on Pharmacotherapy (2009), 10(14), 2221-9

BACKGROUND: Corneocyte accumulation (hyperkeratosis, xerosis) commonly occurs in the stratum corneum (SC) of the feet of diabetic patients, as well as menopausal women. OBJECTIVE: To compare the effects ... [more ▼]

BACKGROUND: Corneocyte accumulation (hyperkeratosis, xerosis) commonly occurs in the stratum corneum (SC) of the feet of diabetic patients, as well as menopausal women. OBJECTIVE: To compare the effects of a 2.5% chitin-glucan formulation with its placebo, and commercially available glycerol formulations. METHODS: This two-step controlled double-blind, randomized, intra-individual study was performed in 30 type 1 and 2 diabetic menopausal women suffering from xerosis of the feet. The formulations were applied once daily for 3 weeks. Electrometric assessments were performed on three sites of the feet at entry in the study, at weekly intervals during the treatment phase, and in a 2-week follow-up out of treatment. Positive controls consisted in two commercially available formulations enriched in glycerol. RESULTS: Data revealed an unequivocal benefit provided by the 2.5% chitin-glucan formulation compared with placebo. The electrometric values were significantly higher at each evaluation time during both treatment and follow-up phases. The two glycerol-enriched formulations showed slightly different kinetics of SC moisturization. A steep increase was followed by a plateau level and a rapid decline after stopping the treatments. CONCLUSION: The increased moisturization of the SC of the sole probably improves the desquamation process and reduces xerosis of the soles. [less ▲]

Detailed reference viewed: 56 (8 ULg)
Full Text
Peer Reviewed
See detailFungal contamination in barley and Kashin-Beck disease in Tibet
Chasseur, Camille; Suetens, Carl; Nolard, Nicole et al

in Lancet (1997), 350

Detailed reference viewed: 10 (2 ULg)
See detailFungal gene expression in a forest soil
Kellner, Harald ULg; Portetelle, Daniel ULg; Vandenbol, Micheline ULg

Poster (2010, August)

Detailed reference viewed: 21 (3 ULg)
Full Text
See detailThe fungal hypotheses (chapter 5.1)
Chasseur, Camille; Lognay, Georges ULg; Suetens, Carl et al

in Big Bone Disease : A multidisciplinary approach of Kasing-Beck disease in Tibet Autonomous Region (P.R. China) (2008)

Detailed reference viewed: 54 (7 ULg)
Full Text
Peer Reviewed
See detailFungal lectin, XCL, is internalized via clathrin-dependent endocytosis and facilitates uptake of other molecules.
Francis, Frédéric ULg; Marty-Detraves, Claire; Poincloux, Renaud et al

in European Journal of Cell Biology (2003), 82(10), 515-22

The lectin isolated from Xerocomus chrysenteron (XCL) displays a toxic activity towards insects. In order to assess its possible mode of action and to gather useful data for its potential use in insect ... [more ▼]

The lectin isolated from Xerocomus chrysenteron (XCL) displays a toxic activity towards insects. In order to assess its possible mode of action and to gather useful data for its potential use in insect-resistant transgenic plants, we investigated the effects of XCL at the cellular level. Immunofluorescence microscopy studies revealed that XCL is rapidly internalized into small endocytic vesicles that further coalesce in the perinuclear region. We show that XCL is endocytosed by the clathrin-dependent pathway, and is delivered to late endosome/lysosome compartments. The internalization of XCL seems to be general since it occurs in different cell types such as insect (SF9) or mammalian (NIH-3T3 and Hela) cell lines. In the presence of XCL, the uptake of GFP and BSA is greatly enhanced, demonstrating that XCL facilitates endocytosis. Thus, XCL could serve as a delivery agent to facilitate the endocytosis of proteins that do not enter the cell alone. [less ▲]

Detailed reference viewed: 25 (5 ULg)
Full Text
Peer Reviewed
See detailFungal thigmotropism in onychomycosis and in a clear hydrogel pad model.
Pierard, Gérald ULg; Pierard, Claudine ULg; Quatresooz, Pascale ULg

in Dermatology : International Journal for Clinical & Investigative Dermatology (2007), 215(2), 107-13

BACKGROUND: Thigmotropism is a biological characteristic corresponding to the directional growth of cells following topographical guidance cues. This behavior has been shown experimentally with fungal ... [more ▼]

BACKGROUND: Thigmotropism is a biological characteristic corresponding to the directional growth of cells following topographical guidance cues. This behavior has been shown experimentally with fungal hyphae of both dermatophytes and nondermatophyte molds, as well as with the mycelial phase of the dimorphic yeast Candida albicans. OBJECTIVE AND METHODS: We presently document this phenomenon using histomycology in onychomycoses of various fungal origins. RESULTS: This mechanism is involved in the invasive phase of the pathogen or opportunistic fungi, and it probably governs various clinical aspects of onychomycoses. We incidentally disclosed fungal invasions of hydrogel pads. Conclusion: Thigmotropism can in part explain the diversity of orientations and shapes of fungi invading nail plates. The same phenomenon was disclosed inside hydrogel pads. As this material is transparent and easy to cut for microscopic examination, fungal thigmotropism is conveniently explored by this way. [less ▲]

Detailed reference viewed: 59 (1 ULg)
Full Text
See detailFungi die onychomycosis veroorzaken in Nederland
Nikkels, Arjen ULg; Arrese Estrada, Jorge ULg; Pierard, Gérald ULg

in Nederlands Tijdschrift voor Geneeskunde (1995), 139

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailFungi occurring on water hyacinth [Eichhornia crassipes (Martius) Solms-Laubach] in Niger River in Mali and their evaluation as mycoherbicides
Dagno, Karim ULg

in Journal of Aquatic Plant Management (2012), 50

One hundred sixteen fungal isolates in seven genera were recovered from water hyacinth plants having pronounced blight symptoms collected in Mali. Isolation frequency of the genera was: Curvularia (60.32 ... [more ▼]

One hundred sixteen fungal isolates in seven genera were recovered from water hyacinth plants having pronounced blight symptoms collected in Mali. Isolation frequency of the genera was: Curvularia (60.32%), Fusarium (42.92%), Alternaria (11.6%), Coniothyrium (11.6%), Phoma (3.48%), Stemphylium (3.48%), and Cadophora (1.16%). On the basis of in vivo pathogenicity tests in which the diseased leaf area percentage and disease severity were visually estimated using a disease severity index, three isolates, Fusarium sp. Mln799, Cadophora sp. Mln715 and Alternaria sp. Mlb684 caused severe disease. These were later identified as Gibberella sacchari Summerell & J.F. Leslie, Cadophora malorum (Kidd & Beaumont) W. Grams, and Alternaria sp. respectively. This is the first report to highlight of C. malorum as a candidate biocontrol agent against water hyacinth. Neither C. malorum Mln715 nor Alternaria sp. Mlb684 in host specificity tests showed any pathogenicity towards 17 crop plants of economic importance in Mali. [less ▲]

Detailed reference viewed: 85 (5 ULg)
Full Text
Peer Reviewed
See detailFungi occurring on waterhyacinth (Eichhornia crassipes (Martius) Solms-Laubach) in Niger River in Mali and their evaluation as mycoherbicides.
Dagno, Karim; Lahlali, Rachid; Diourté, Mamourou et al

in Journal of Aquatic Plant Management (2012), 50

We recovered 116 fungal isolates in 7 genera from waterhyacinth plants having pronounced blight symptoms collected in Mali. Isolation frequency of the genera was Curvularia (60.32%), Fusarium (42.92 ... [more ▼]

We recovered 116 fungal isolates in 7 genera from waterhyacinth plants having pronounced blight symptoms collected in Mali. Isolation frequency of the genera was Curvularia (60.32%), Fusarium (42.92%), Alternaria (11.6%), Coniothyrium (11.6%), Phoma (3.48%), Stemphylium (3.48%), and Cadophora (1.16%). On the basis of in vivo pathogenicity tests in which the diseased leaf area percentage and disease severity were visually estimated using a disease severity index, three isolates, Fusarium sp. Mln799, Cadophora sp. Mln715, and Alternaria sp. Mlb684 caused severe disease. These were later identified as Gibberella sacchari Summerell & J.F. Leslie, Cadophora malorum (Kidd & Beaumont) W. Grams, and Alternaria sp. respectively. This is the first report to highlight C. malorum as a candidate biocontrol agent against waterhyacinth. Neither C. malorum Mln715 nor Alternaria sp. Mlb684 in host specificity tests showed any pathogenicity toward 17 crop plants of economic importance in Mali. [less ▲]

Detailed reference viewed: 62 (0 ULg)
Full Text
Peer Reviewed
See detailFungi Unearthed: Transcripts Encoding Lignocellulolytic and Chitinolytic Enzymes in Forest Soil
Kellner, Harald ULg; Zak, Don; Vandenbol, Micheline ULg

in PLoS ONE (2010), 5(Issue 6), 10971-7

Detailed reference viewed: 37 (7 ULg)
See detailFunktioneel impakt van de ademhalingsziekten bij runderen
Lekeux, Pierre ULg

in Vlaams Diergeneeskundig Tijdschrift (1989), 58

Detailed reference viewed: 21 (2 ULg)
Peer Reviewed
See detailFunktioneller Zugang zu den Respirationskrankheiten der Doppellender-Rinder der Rasse Weiss-Blaue Belgier.
Gustin, Pascal ULg; Bakima, M.; Lekeux, Pierre ULg et al

in Deutsche Tierarztliche Wochenschrift (1987), 94(5), 261-264

Detailed reference viewed: 31 (0 ULg)
Full Text
See detailFunktionen von Intertextualität in „Zur Chronik von Grieshuus“ und „Zur ‚Wald- und Wasserfreude‘“
Gerrekens, Louis ULg

in Jackson, David; Pastor, Eckart; Eversberg, Gerd (Eds.) Stormlektüren. Festschrift für K. E. Laage zum 80. Geburtstag. (2000)

Detailed reference viewed: 32 (3 ULg)
See detailFunktionsanalyse und Unterrichtsevaluation
de Landsheere, Gilbert ULg

in Petillon, Hanns; Wagner, Jürgen W.L.; Wolf, Bernhard (Eds.) Schülergerechte Diagnose : theoretische und empirische Beiträge zur pädagogischen Diagnostik : Festschrift zum 60. Geburtstag von Karlheinz Ingenkamp (1986)

Detailed reference viewed: 9 (0 ULg)
See detailFunktionswörter
Möller, Robert ULg

in Hufeisen, Britta; Marx, Nicole (Eds.) EuroComGerm - Die sieben Siebe: Germanische Sprachen lesen lernen (2007)

Detailed reference viewed: 30 (1 ULg)