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See detailFunctional screening of a winter and a spring genomic DNA libraries obtained from soils in a winter wheat crop
Stroobants, Aurore ULg; Portetelle, Daniel ULg; Vandenbol, Micheline ULg

Poster (2013, June 10)

Soils are very rich environments where the diversity of microorganisms is very high. These microorganisms play important role in the degradation of organic matter with enzymes able to degrade it. The aim ... [more ▼]

Soils are very rich environments where the diversity of microorganisms is very high. These microorganisms play important role in the degradation of organic matter with enzymes able to degrade it. The aim of this work is to discover by functional screening new enzymatic activities of microorganisms from soils collected in winter and spring in a winter wheat crop. The genomic DNA was extracted from both soils to construct two libraries in Escherichia coli. These libraries were then screened for several enzymes such as lipase, beta-glucosidase, cellulase, α-amylase,… At this time, 2 beta-glucosidases and 3 lipases have already been found in the winter library and 3 beta-glucosidases and 1 lipase in the spring library. Sequence analyses with the BLASTX program revealed that two beta-glucosidases have less than 65% of sequence identity with known beta-glucosidases, one have 64% of identity with a known beta-galactosidase and one have 59% of identity with a glycoside hydrolase. The fifth seems to be a phosphorylase kinase (54% identity) which have a glucoamylase domain responsible for the activity. This ORF is interrupted by a transposase. Three of the four lipases have less than 60% of sequence identity with known lipases/esterases. The fourth show 55% of identity with a known beta-lactamase. [less ▲]

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See detailFunctional Shape Matching and Fuzzy Templates
Delanoy, Richard L.; Verly, Jacques ULg; Dudgeon, Dan E.

Conference (1991, September)

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See detailThe functional significance of E-b-Farnesene: Does it influence the populations of aphid natural enemies in the fields?
Cui, L-L; Francis, Frédéric ULg; Heuskin, Stéphanie ULg et al

in Biological Control (2012)

Aphids cause much damage to Chinese cabbage in northern China. Over reliance on pesticides have large environmental and human health costs that compel researchers to seek alternative management tactics ... [more ▼]

Aphids cause much damage to Chinese cabbage in northern China. Over reliance on pesticides have large environmental and human health costs that compel researchers to seek alternative management tactics for aphid control. The component of aphid alarm pheromone, E-b-Farnesene (EbF), extracted from Matricaria chamomilla L., which attracts natural enemies in the laboratory, may have significant implications for the design of cabbage aphid control strategies. The purpose of this paper is to understand the effects of EbF on natural enemies to cabbage aphid control in Chinese cabbage fields. Ladybeetles on Chinese cabbage leaves in EbF released plots and Aphidiidae in EbF released yellow traps were significantly higher than those of in controls. No significant differences were detected in the interactions of different treatments and the two years for all natural enemies. More important, lower aphid densities were found in EbF released plots. Our results suggested that the EbF extracted from M. chamomilla L. could attract natural enemies to reduce cabbage aphids in the Chinese cabbage fields. [less ▲]

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See detailFunctional significance of the rapid regulation of brain estrogen action: Where do the estrogens come from?
Cornil, Charlotte ULg; Ball, G. F.; Balthazart, Jacques ULg

in Brain Research (2006), 1126

Estrogens exert a wide variety of actions on reproductive and non-reproductive functions. These effects are mediated by slow and long lasting genomic as well as rapid and transient non-genomic mechanisms ... [more ▼]

Estrogens exert a wide variety of actions on reproductive and non-reproductive functions. These effects are mediated by slow and long lasting genomic as well as rapid and transient non-genomic mechanisms. Besides the host of studies demonstrating the role of genomic actions at the physiological and behavioral level, mounting evidence highlights the functional significance of non-genomic effects. However, the source of the rapid changes in estrogen availability that are necessary to sustain their fast actions is rarely questioned. For example, the rise of plasma estrogens at pro-estrus that represents one of the fastest documented changes in plasma estrogen concentration appears too slow to explain these actions. Alternatively, estrogen can be synthesized in the brain by the enzyme aromatase providing a source of locally high concentrations of the steroid. Furthermore, recent studies demonstrate that brain aromatase can be rapidly modulated by afferent inputs, including glutamatergic afferents. A role for rapid changes in estrogen production in the central nervous system is supported by experiments showing that acute aromatase inhibition affects nociception as well as male sexual behavior and that preoptic aromatase activity is rapidly (within min) modulated following mating. Such mechanisms thus fulfill the gap existing between the fast actions of estrogen and their mode of production and open new avenues for the understanding of estrogenic effects on the brain. (c) 2006 Elsevier B.V. All rights reserved. [less ▲]

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See detailFunctional Sites Of The Bovine Leukemia-Virus Envelope Glycoproteins Using Structural And Immunological Data
Callebaut, I.; Mornon, Jp.; Burny, A. et al

in Archives Internationales de Physiologie de Biochimie et de Biophysique (1994), 102(2),

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See detailFunctional specialization for auditory-spatial processing in the occipital cortex of congenitally blind humans
Collignon, Olivier; Vandewalle, Gilles ULg; Voss, Patrice et al

in Proceedings of the National Academy of Sciences of the United States of America (2011), 108(11), 4435-40

The study of the congenitally blind (CB) represents a unique opportunity to explore experience-dependant plasticity in a sensory region deprived of its natural inputs since birth. Although several studies ... [more ▼]

The study of the congenitally blind (CB) represents a unique opportunity to explore experience-dependant plasticity in a sensory region deprived of its natural inputs since birth. Although several studies have shown occipital regions of CB to be involved in nonvisual processing, whether the functional organization of the visual cortex observed in sighted individuals (SI) is maintained in the rewired occipital regions of the blind has only been recently investigated. In the present functional MRI study, we compared the brain activity of CB and SI processing either the spatial or the pitch properties of sounds carrying information in both domains (i.e., the same sounds were used in both tasks), using an adaptive procedure specifically designed to adjust for performance level. In addition to showing a substantial recruitment of the occipital cortex for sound processing in CB, we also demonstrate that auditory-spatial processing mainly recruits the right cuneus and the right middle occipital gyrus, two regions of the dorsal occipital stream known to be involved in visuospatial/motion processing in SI. Moreover, functional connectivity analyses revealed that these reorganized occipital regions are part of an extensive brain network including regions known to underlie audiovisual spatial abilities (i.e., intraparietal sulcus, superior frontal gyrus). We conclude that some regions of the right dorsal occipital stream do not require visual experience to develop a specialization for the processing of spatial information and to be functionally integrated in a preexisting brain network dedicated to this ability. [less ▲]

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See detailFunctional specifications of Teledu : ELECTRA deliverable D07.02
Baldewyns, Laurence; Reggers, Thérèse ULg; Poumay, Marianne ULg et al

Report (1997)

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See detailFunctional study of Arabidopsis thaliana ASF/SF2-like pre-mRNA SR splicing factors
Stankovic, Nancy ULg; Tillemans, Vinciane; Leponce, Isabelle et al

Poster (2012, July 04)

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See detailFunctional study of Arabidopsis thaliana ASF/SF2-like pre-mRNA SR splicing factors
Stankovic, Nancy ULg; Tillemans, Vinciane; Hanikenne, Marc et al

Poster (2010, January 26)

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See detailFunctional study of human tonsillar follicular dendritic cells.
Simar, L. J.; Lilet, Chantal ULg; Heinen, Ernst ULg et al

in Acta Oto-Rhino-Laryngologica Belgica (1984), 38(3), 278-87

Follicular dendritic cells (FDC) isolated from human tonsils and adenoids appear as round clusters where the FDC surround lymphoid cells. Using fluorescein and colloidal gold labelled antibodies we ... [more ▼]

Follicular dendritic cells (FDC) isolated from human tonsils and adenoids appear as round clusters where the FDC surround lymphoid cells. Using fluorescein and colloidal gold labelled antibodies we determined their surface antigens, the immunoglobulins they fix and the cells they envelope. Isolated FDC react with specific anti-FDC antibodies but also with an anti-monocyte/macrophage antibody and with anti-HLA-DR antibodies. They retain IgG, IgM, IgA and IgE but not IgD immunoglobulins; this retention occurs, according to our results, via their Fc and C3b receptors. The cells they envelope are mainly B cells, but also occasionally T helper cells. T suppressor cells were only rarely found in contact with FDC. We suggest that FDC create a micro-environment favourable to the proliferation and differentiation of B cells during the humoral response. [less ▲]

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See detailFunctional study of the pectoral spine stridulation mechanism in different mochokid catfishes
Parmentier, Eric ULg; Fabri, Grégory ULg; Kaatz, Ingrid et al

in Journal of Experimental Biology (2010), 213

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See detailFunctional study of the Ser/Arg-rich splicing factor SRSF5a during zebrafish embryonic development
Joris, Marine ULg; Muller, Marc ULg; Motte, Patrick ULg

Poster (2014, June 05)

Nuclear pre-mRNA splicing is a key process regulating gene expression in eukaryotes. Splicing consists in the removal of introns and the joining of exons within a dynamic macromolecular complex called the ... [more ▼]

Nuclear pre-mRNA splicing is a key process regulating gene expression in eukaryotes. Splicing consists in the removal of introns and the joining of exons within a dynamic macromolecular complex called the spliceosome, which consist of five small nuclear ribonucleoproteins (snRNPs) and numerous non snRNPs proteins (1). Amongst these non snRNPs proteins, the SR proteins family constituted an important group of splicing factors that are involved in constitutive and alternative splicing (2,3). SR proteins are structurally related as they are characterized by one or two RNA-recognition motifs (RRMs) in N-ter and a C-terminal domain enriched in dipeptide Ser/Arg. Phylogenetic inference using the RRM domain allowed us to identify 13 encoding genes for SR proteins in the vertebrate model organism, Danio rerio. The Zebrafish is increasingly recognized as a powerful model for the study of vertebrate embryonic development in a physiological context. The roles of SR splicing factors during animal cell differentiation and development are largely unknown. The aim of the present research is to investigate SR proteins functions during zebrafish development by using molecular and genetic approaches. In this study, we investigated the role of the SR splicing factor SRSF5a. The expression profile was determined by in situ hybridization at 24, 48 and 72 hours post-fertilization and showed SRSF5a expression mainly in brain, retina and pharyngeal arches at these stages. Furthermore, SRSF5a knock-down by morpholinos microinjection strongly suggests an important role of this specific splicing factor during eyes and brain development. In order to gain insight into the molecular function of SRSF5a, we analysed control and morphant transcriptomes using high throughput RNA sequencing. Finally, we use a complementary approach to morpholinos and generate SRSF5a mutant fishes using TALENs (Tal effector nucleases)(4,5). 1. Wahl MC, Will CL, & Luhrmann R (2009) The spliceosome: design principles of a dynamic RNP machine. Cell 136(4):701-718. 2. Long JC & Caceres JF (2009) The SR protein family of splicing factors: master regulators of gene expression. Biochem J 417(1):15-27. 3. Graveley BR (2000) Sorting out the complexity of SR protein functions. RNA 6(9):1197-1211. 4. Moore et al. (2012) Improved Somatic Mutagenesis in Zebrafish Using Transcription Activator-Like Effector Nucleases (TALENs). Plos One 120(1):1-12. 5. Cade et al (2012) Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs. Nucleic acid research Vol 40, No 16:8001-8010. [less ▲]

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See detailFunctional study of the Ser/Arg-rich splicing factor SRSF5a during zebrafish embryonic development.
Joris, Marine ULg; Larbuisson, Arnaud ULg; Muller, Marc ULg et al

Poster (2013, April 18)

To investigate the role of the splicing factor SRSF5a during zebrafish embryonic development, we performed SRSF5a knockdown by morpholino microinjection and we analysed control and morphant transcriptomes ... [more ▼]

To investigate the role of the splicing factor SRSF5a during zebrafish embryonic development, we performed SRSF5a knockdown by morpholino microinjection and we analysed control and morphant transcriptomes using RNA sequencing. [less ▲]

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See detailFunctional suppression of the yeast msb3- mutation
Biver, Sophie ULg; Vandenbol, Micheline ULg

Poster (2010, September)

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See detailFunctional Templates and Their Applications to 3-D Object Recognition
Delanoy, Richard L.; Verly, Jacques ULg; Dudgeon, Dan E.

Conference (1992, March)

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See detailA functional test procedure for the improved commissioning of a VAV system
Andre, Philippe ULg; Lacôte, P.; Aparecida Silva, Cl. et al

Conference (2004, October)

In the context of the IEA40 research project, a specification of a “Functional Test Procedure” was progressively developed using the CA-MET building in Namur as real size test bench for the application ... [more ▼]

In the context of the IEA40 research project, a specification of a “Functional Test Procedure” was progressively developed using the CA-MET building in Namur as real size test bench for the application and validation of the procedure. The target system is a typical VAV system devoted to the ventilation, cooling and air conditioning functions. The procedure consists in a series of operations to be applied to the system, considered as a set of connected components and taking into account the following constraints: - time availability - intrinsic limitations of the system - reference performances The proposed procedure is divided in two sets of actions: - a number of preliminary actions allowing to take the best benefit of the subsequent phases: o identification of available measurement points o identification of actuators characteristics o identification of control strategies o aeraulic network calculations o selection of measurement techniques and experimental design - the verification tasks themselves: The philosophy of the verification process is to bring the system into a number of states whoch allowed each specific checks to be carried out. The organisation of the procedure tries to optimize the time spent for commissioning by logically sequencing the operations according to the following order: - test in manual operation - test in manual stop - test in normal operation - test at maximum flowrate - test at minimum flowrate - test in automatic stop The paper will provide the specific operations to be carried out at each step and will illustrate the whole process with the prototyping work performed on the CA-MET building in Namur. [less ▲]

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See detailFunctional traits are weak predictors of tree sapling growth worldwide
Paine, C. E. Timothy; Auge, Harald; Baraloto, Christopher et al

Conference (2013, June 26)

Functional traits, well-defined characteristics of organisms that influence or coordinate with ecological performance, promise that one may predict vital rates, such as survival and growth, by measuring a ... [more ▼]

Functional traits, well-defined characteristics of organisms that influence or coordinate with ecological performance, promise that one may predict vital rates, such as survival and growth, by measuring a few aspects of anatomy and physiology. Functional traits have been shown to correlate significantly, though weakly, with the relative growth rate (RGR) of trees in five Neotropical forests. Here, we estimated size-standardized height RGR for 26344 saplings of 240 species from 23 sites around the world using nonlinear models of growth. We then predicted the contribution of three functional traits (specific leaf area, wood density and seed mass) to individual RGR with a hierarchical Bayesian model. The three functional traits, alone and in combination, explained a relatively small amount of variance in RGR, with variation among continents and between temperate and tropical regions. We echo the calls for researchers to shift toward the measurement of 'hard' functional traits, with clear linkages to realized vital rates, rather than 'soft' functional traits, which are easier to measure but may only indicate the theoretical maxima of vital rates. Doing so would advance ecology onto a more solidly functional foundation. [less ▲]

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See detailA functional variant of lymphoid tyrosine phosphatase is associated with type I diabetes.
Bottini, Nunzio; Musumeci, Lucia; Alonso, Andres et al

in Nature Genetics (2004), 36(4), 337-8

We report that a single-nucleotide polymorphism (SNP) in the gene (PTPN22) encoding the lymphoid protein tyrosine phosphatase (LYP), a suppressor of T-cell activation, is associated with type 1 diabetes ... [more ▼]

We report that a single-nucleotide polymorphism (SNP) in the gene (PTPN22) encoding the lymphoid protein tyrosine phosphatase (LYP), a suppressor of T-cell activation, is associated with type 1 diabetes mellitus (T1D). The variants encoded by the two alleles, 1858C and 1858T, differ in a crucial amino acid residue involved in association of LYP with the negative regulatory kinase Csk. Unlike the variant encoded by the more common allele 1858C, the variant associated with T1D does not bind Csk. [less ▲]

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See detailFunctional, inflammatory and morphological characterisation of a cat model of allergic airway inflammation
Kirschvink, N.; Leemans, Jérôme ULg; Delvaux, François et al

in Veterinary Journal (2007), 174(3), 541-553

The aims of this study were to characterise a model of feline allergic airway inflammation and to test through a longitudinal investigation whether five repeated allergen exposures would lead to signs of ... [more ▼]

The aims of this study were to characterise a model of feline allergic airway inflammation and to test through a longitudinal investigation whether five repeated allergen exposures would lead to signs of airway remodelling that would be detectable in vivo. Eight healthy control cats and eight cats sensitised with Ascaris suum allergens were investigated. Barometric whole body plethysmography (BWBP) was used for the assessment of respiratory variables and airway responsiveness (AR). Bronchoalveolar lavage fluid (BALF) was sampled for cytology and determination of F-2-isoprostane concentration and matrix metalloproteinase type 9 (MMP-9) activity. Thoracic radiography and bronchoscopy scores were also established. Cats were investigated prior to sensitisation and after inhalation of placebo or allergen challenge 1. BWBP measurements revealed a significant increase of enhanced pause (Penh), an index of bronchoconstriction, and AR in sensitised cats in response to allergen challenge 1. A significant increase in BALF neutrophil and eosinophil %, F-2-isoprostane concentration and MMP-9 activity, and increased radiography and bronchoscopy scores were recorded. After a recovery period of 6 weeks, all variables except BALF MMP-9 returned to baseline values. Four further allergen challenges induced similar changes to those seen in challenge I and no signs of persistent changes suggestive of bronchial remodelling were detectable. The model provides an in vivo approach to functional, inflammatory and morphological changes occurring in response to single and repeated allergen exposure. [less ▲]

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See detailFunctionalisation and chemical characterisation of cellulose derived carbon aerogels
Grzyb, Bartosz; Hildenbrand, Claudia; Berthon-Fabry, Sandrine et al

in Carbon (2010), 48

Detailed reference viewed: 34 (2 ULg)