Foi et démocratie. Débat contradictoire avec le R.P. Ignace Berten, O.P.
Conference given outside the academic context (2008)Detailed reference viewed: 7 (0 ULg)
Foi et Modernité aux XIXe-XXe s. : quelle(s) transaction(s) ?
Scientific conference (1993, September 15)Detailed reference viewed: 4 (0 ULg)
Folate receptor autoantibodies and spinal fluid 5-methyltetrahydrofolate deficiency in Rett syndrome
Ramaekers, Vincent ; ; et al
in Neuropediatrics (2007), 38(4), 179-183
Rett syndrome was associated with low cerebrospinal fluid (CSF) 5-methyltetrahydrofolate (5MTHF) in 42-50% of European patients whereas approximately 93% of the patients from North-America had a normal ... [more ▼]
Rett syndrome was associated with low cerebrospinal fluid (CSF) 5-methyltetrahydrofolate (5MTHF) in 42-50% of European patients whereas approximately 93% of the patients from North-America had a normal CSF 5MTHF status. We determined the CSF folate status in Rett patients living in North- and South-Western Europe and measured serum folate receptor (FR) autoantibodies of the blocking type to explain the reduced folate transport across the choroid plexus. Irrespective of their MECP2 genotype and despite normal plasma folate values, 14 of 33 Rett patients (42%) had low CSF folate levels. Blocking FR autoantibodies were found in 8 of the Rett patients (24%), 6 of whom had low CSF folate levels. FR autoimmunity was primarily found within the group of Rett patients with low CSF folate status with a higher incidence in North-Western Europe. In Rett patients from North-America 74 of 76 girls had higher folate values in both serum and CSF than European patients. The food folate fortification in North-America may account for the higher folate levels and may prevent CFD in these Rett patients. FR autoimmunity occurred predominantly in Rett patients from North-Western Europe and may contribute to cerebral folate deficiency (CFD). © Georg Thieme Verlag KG Stuttgart. [less ▲]Detailed reference viewed: 6 (1 ULg)
Folate receptor autoimmunity and cerebral folate deficiency in low-functioning autism with neurological deficits
RAMAEKERS, Vincent ; ; et al
in Neuropediatrics (2007), 38(6), 276-281
Reduced folate transport to the CNS was identified in two autism spectrum disorders, i.e., Rett syndrome and infantile low-functioning autism with neurological abnormalities. Twenty-five patients with ... [more ▼]
Reduced folate transport to the CNS was identified in two autism spectrum disorders, i.e., Rett syndrome and infantile low-functioning autism with neurological abnormalities. Twenty-five patients with early-onset low-functioning autism with or without neurological deficits, were evaluated for serum folate, cerebrospinal fluid (CSF) 5-methyltetrahydrofolate (5MTHF), and serum FR autoantibodies of the blocking type to determine the significance of folate receptor (FR) autoantibodies with respect to folate transport across the blood-CSF barrier. In spite of normal serum folate, CSF 5MTHF was low in 23 of 25 patients. The reduced CSF folate in 19 of these 23 patients could be explained by serum FR autoantibodies blocking the folate binding site of the membrane-attached FR on the choroid epithelial cells. Oral folinic acid supplements led to normal CSF 5MTHF and partial or complete clinical recovery after 12 months. Serum FR autoimmunity appears to represent an important factor in the pathogenesis of reduced folate transport to the nervous system among children with early-onset low-functioning autism associated with or without neurological deficits. Early detection of FR autoantibodies may be a key factor in the prevention and therapeutic intervention among this subgroup of patients with autism. © Georg Thieme Verlag KG Stuttgart. [less ▲]Detailed reference viewed: 82 (1 ULg)
Folding and Stability of Class A beta-Lactamases
Matagne, André ;
in Frère, Jean-Marie (Ed.) Beta-Lactamases (2012)
Class A β-lactamases have proved useful as model proteins in studying a wide variety of aspects of protein folding. We review those features that have shed light on kinetic intermediates that take part in ... [more ▼]
Class A β-lactamases have proved useful as model proteins in studying a wide variety of aspects of protein folding. We review those features that have shed light on kinetic intermediates that take part in folding, including some insight into the molecular basis of the kinetic and stable molten-globule states that have been identified. The contrast between the folding behaviour of PC1 and the two lactamase mutants, P54 and P2, can be attributed in some detail to changes in molecular conformation. The early, very rapid stages of folding of β-lactamases have been shown to be multiphasic, and an interesting intermediate is described that has non-native contacts involving burial of the C-terminal tryptophan. A further feature is that the region around the disulphide bond in the TEM enzymes is formed very early in the foldingreaction. The unusual feature of class A β-lactamases, i.e. a cispeptide bond critical in the stereochemistry of the active site that usually, but not always, involves a proline residue, has been shown to be important in accounting for the slow folding reactions. The effect of substrates on the stabilization of the enzymes and on their reversible deactivation is also reviewed. [less ▲]Detailed reference viewed: 12 (1 ULg)
Folding mechanism of single domain antibody fragments (VHHs) and influence of the internal disulphide bridge on their stability and folding pathway.
Poster (2009, February)Detailed reference viewed: 15 (3 ULg)
Folding of class A beta-lactamases is rate-limited by peptide bond isomerization and occurs via parallel pathways.
Vandenameele, Julie ; Lejeune, Annabelle ; Di Paolo, Alexandre et al
in Biochemistry (2010), 49(19), 4264-75
Class A beta-lactamases (M(r) approximately 29000) provide good models for studying the folding mechanism of large monomeric proteins. In particular, the highly conserved cis peptide bond between residues ... [more ▼]
Class A beta-lactamases (M(r) approximately 29000) provide good models for studying the folding mechanism of large monomeric proteins. In particular, the highly conserved cis peptide bond between residues 166 and 167 at the active site of these enzymes controls important steps in their refolding reaction. In this work, we analyzed how conformational folding, reactivation, and cis/trans peptide bond isomerizations are interrelated in the folding kinetics of beta-lactamases that differ in the nature of the cis peptide bond, which involves a Pro167 in the BS3 and TEM-1 enzyme, a Leu167 in the NMCA enzyme, and which is missing in the PER-1 enzyme. The analysis of folding by spectroscopic probes and by the regain of enzymatic activity in combination with double-mixing procedures indicates that conformational folding can proceed when the 166-167 bond is still in the incorrect trans form. The very slow trans --> cis isomerization of the Glu166-Xaa167 peptide bond, however, controls the final step of folding and is required for the regain of the enzymatic activity. This very slow phase is absent in the refolding of PER-1, in which the Glu166-Ala167 peptide bond is trans. The double-mixing experiments revealed that a second slow kinetic phase is caused by the cis/trans isomerization of prolines that are trans in the folded proteins. The folding of beta-lactamases is best described by a model that involves parallel pathways. It highlights the role of peptide bond cis/trans isomerization as a kinetic determinant of folding. [less ▲]Detailed reference viewed: 52 (14 ULg)
The Folding Process of Hen Lysozyme: A Perspective from the 'New View'
Matagne, André ;
in Cellular and Molecular Life Sciences : CMLS (1998), 54(4), 363-71
How a conformationally disordered polypeptide chain rapidly and efficiently achieves its well-defined native structure is still a major question in modern structural biology. Although much progress has ... [more ▼]
How a conformationally disordered polypeptide chain rapidly and efficiently achieves its well-defined native structure is still a major question in modern structural biology. Although much progress has been made towards rationalizing the principles of protein structure and dynamics, the mechanism of the folding process and the determinants of the final fold are not yet known in any detail. One protein for which folding has been studied in great detail by a combination of diverse techniques is hen lysozyme. In this article we review the present state of our knowledge of the folding process of this enzyme and focus in particular on recent experiments to probe some of its specific features. These results are then discussed in the context of the 'new view' of protein folding based on energy surfaces and landscapes. It is shown that a schematic energy surface for lysozyme folding, which is broadly consistent with our experimental data, begins to provide a unified model for protein folding through which experimental and theoretical ideas can be brought together. [less ▲]Detailed reference viewed: 32 (8 ULg)
"La folie heureuse" : à propos d'un passage d'Horace (Épîtres II, 2, v. 128-140)
in Filiber, Carine (Ed.) Vertiges de la folie. Catalogue de l'exposition (Musée de la Vie Wallonne, Liège, 30/03-19/08/2012) (2012)Detailed reference viewed: 20 (7 ULg)
Foliicolous lichens from Madeira, with the description of a new genus and two new species and a world-wide key to foliicolous Fellhanera
in Lichenologist (1996), 28Detailed reference viewed: 5 (0 ULg)
Foliicolous lichens from Southeastern United States
in Bryologist (1979), 82Detailed reference viewed: 9 (0 ULg)
Foliicolous lichens: ecological and chorological data
in Botanical Journal of the Linnean Society (1986), 100Detailed reference viewed: 5 (0 ULg)
Folinic acid treatment for schizophrenia associated with folate receptor autoantibodies.
; ; et al
in Molecular genetics and metabolism (2014), 113(4), 307-14
BACKGROUND: Auto-antibodies against folate receptor alpha (FRalpha) at the choroid plexus that block N(5)-methyltetrahydrofolate (MTHF) transfer to the brain were identified in catatonic schizophrenia ... [more ▼]
BACKGROUND: Auto-antibodies against folate receptor alpha (FRalpha) at the choroid plexus that block N(5)-methyltetrahydrofolate (MTHF) transfer to the brain were identified in catatonic schizophrenia. Acoustic hallucinations disappeared following folinic acid treatment. Folate transport to the CNS prevents homocysteine accumulation and delivers one-carbon units for methyl-transfer reactions and synthesis of purines. The guanosine derivative tetrahydrobiopterin acts as common co-factor for the enzymes producing dopamine, serotonin and nitric oxide. METHODS: Our study selected patients with schizophrenia unresponsive to conventional treatment. Serum from these patients with normal plasma homocysteine, folate and vitamin B12 was tested for FR autoantibodies of the blocking type on serial samples each week. Spinal fluid was analyzed for MTHF and the metabolites of pterins, dopamine and serotonin. The clinical response to folinic acid treatment was evaluated. RESULTS: Fifteen of 18 patients (83.3%) had positive serum FR auto-antibodies compared to only 1 in 30 controls (3.3%) (chi(2)=21.6; p<0.0001). FRalpha antibody titers in patients fluctuated over time varying between negative and high titers, modulating folate flux to the CNS, which explained low CSF folate values in 6 and normal values in 7 patients. The mean+/-SD for CSF MTHF was diminished compared to previously established controls (t-test: 3.90; p=0.0002). A positive linear correlation existed between CSF MTHF and biopterin levels. CSF dopamine and serotonin metabolites were low or in the lower normal range. Administration of folinic acid (0.3-1mg/kg/day) to 7 participating patients during at least six months resulted in clinical improvement. CONCLUSION: Assessment of FR auto-antibodies in serum is recommended for schizophrenic patients. Clinical negative or positive symptoms are speculated to be influenced by the level and evolution of FRalpha antibody titers which determine folate flux to the brain with up- or down-regulation of brain folate intermediates linked to metabolic processes affecting homocysteine levels, synthesis of tetrahydrobiopterin and neurotransmitters. Folinic acid intervention appears to stabilize the disease process. [less ▲]Detailed reference viewed: 4 (0 ULg)
Folipidine, a new type quinoline alkaloid from plants of the Haplophyllum genus
; ; et al
in Chemistry of Natural Compounds (2005), 41(1, JAN-FEB), 60-64
The new quinoline alkaloid folipidine, the structure of which was established by chemical transformations and spectral data (UV, IR, mass, NMR) using APT 2D H-1-H-1 COSY, NOESY, and H-1-(13)CHSQC, HMBC ... [more ▼]
The new quinoline alkaloid folipidine, the structure of which was established by chemical transformations and spectral data (UV, IR, mass, NMR) using APT 2D H-1-H-1 COSY, NOESY, and H-1-(13)CHSQC, HMBC, was isolated from two plants of the Haplophyllum mints. Folipidine is the first representative of a new type of quinoline alkaloids that contain a heteroaromatic skeleton of [3,4-b]conjugated pyrrole and quinoline fragments. The total alkaloids of these plants exhibit antitumor activity. Folipidine does not possess such activity. [less ▲]Detailed reference viewed: 30 (0 ULg)