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See detailThe fully-active and structurally-stable form of the mitochondrial ATP synthase of Polytomella sp is dimeric
Villavicencio-Queijeiro, Alexa; Vazquez-Acevedo, Miriam; Cano-Estrada, Araceli et al

in Journal of Bioenergetics & Biomembranes (2009), 41(1), 1-13

Mitochondrial F1FO-ATP synthase of chlorophycean algae is a stable dimeric complex of 1,600 kDa. It lacks the classic subunits that constitute the peripheral stator-stalk and the orthodox polypeptides ... [more ▼]

Mitochondrial F1FO-ATP synthase of chlorophycean algae is a stable dimeric complex of 1,600 kDa. It lacks the classic subunits that constitute the peripheral stator-stalk and the orthodox polypeptides involved in the dimerization of the complex. Instead, it contains nine polypeptides of unknown evolutionary origin named ASA1 to ASA9. The isolated enzyme exhibited a very low ATPase activity (0.03 Units/mg), that increased upon heat treatment, due to the release of the F-1 sector. Oligomycin was found to stabilize the dimeric structure of the enzyme, providing partial resistance to heat dissociation. Incubation in the presence of low concentrations of several non-ionic detergents increased the oligomycin-sensitive ATPase activity up to 7.0-9.0 Units/mg. Incubation with 3% (w/v) taurodeoxycholate monomerized the enzyme. The monomeric form of the enzyme exhibited diminished activity in the presence of detergents and diminished oligomycin sensitivity. Cross-linking experiments carried out with the dimeric and monomeric forms of the ATP synthase suggested the participation of the ASA6 subunit in the dimerization of the enzyme. The dimeric enzyme was more resistant to heat treatment, high hydrostatic pressures, and protease digestion than the monomeric enzyme, which was readily disrupted by these treatments. We conclude that the fully-active algal mitochondrial ATP synthase is a stable catalytically active dimer; the monomeric form is less active and less stable. Monomer-monomer interactions could be mediated by the membrane-bound subunits ASA6 and ASA9, and may be further stabilized by other polypeptides such as ASA1 and ASA5. [less ▲]

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See detailFulminant Hepatic Failure Induced by Venlafaxine and Trazodone Therapy: A Case Report.
Detry, Olivier ULg; Delwaide, Jean ULg; De Roover, Arnaud ULg et al

in Transplantation Proceedings (2009), 41(8), 3435-3436

Although acute hepatitis may be a side effect of many medications, most cases are reversible after treatment interruption, and fulminant hepatic failure (FHF) is rare. Venlafaxine and trazodone are 2 ... [more ▼]

Although acute hepatitis may be a side effect of many medications, most cases are reversible after treatment interruption, and fulminant hepatic failure (FHF) is rare. Venlafaxine and trazodone are 2 popular antidepressant agents. Alteration of liver enzyme levels has been reported as a side effect of these drugs at normal doses. Herein we have reported the case of a 48-year-old woman without any previous history of liver disease, who developed fulminant liver failure after 4 months of venlafaxine and trazodone therapy. She required liver transplantation, a procedure that was successful with full patient recovery. The first 5 years of follow-up were uneventful. This case documented that venlafaxine and trazodone at normal doses can produce severe liver toxicity. Liver tests should be monitored regularly in patients who receive this therapy. [less ▲]

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See detailFumetto, Art Brut e dissidenza
Dejasse, Erwin ULg

in Rivista dell’Osservatorio Outsider Art (2011), 3

The article deals with the connections between comics on one hand and art brut and outsider art on the other hand

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See detailFumigant toxicity of Mentha pulegium and Citrus aurantium essential oils to Tetranychus urticae (Acari: Tetranychidae) and Phytoseiulus persimilis (Acari: Phytoseiidae)
Attia, Sabrine; Grissa, K.L.; Lognay, Georges ULg et al

in Boeckx; Bossier, Peter; Smagghe, Guy (Eds.) et al Proceedings of the 65th international symposium on crop protection (2013, May 21)

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See detailFumure azotée : Effet d'un sous-semis sur une culture de maïs
Tychon, Bernard ULg; Capellen, O.; Foucart, G. et al

in Revue de l’Agriculture = Landbouwtijdschrift (1992), 45(2),

La superficie occupée par la culture de maïs en Lorraine belge n'a cessé d'augmenter depuis le début des années 70. Comme toutes les têtes de rotation, le maïs reçoit d'importantes quantités de fumier et ... [more ▼]

La superficie occupée par la culture de maïs en Lorraine belge n'a cessé d'augmenter depuis le début des années 70. Comme toutes les têtes de rotation, le maïs reçoit d'importantes quantités de fumier et de lisier. Ces concentrations élevées en matières organiques associées aux engrais minéraux sont très souvent à l'origine d'un bilan azoté déséquilibré. Dans ces conditions, les reliquats en azote généralement observés dans le sol au moment de la récolte constituent un danger réel pour la qualité des nappes phréatiques. Dans cet article, nous étudions un sous-semis de ray-grass (Lolium perenne L.). Le suivi concerne d'abord la compétition entre la culture de maïs et le sous-semis. Pour les conditions pédoclimatiques de l'essai, le sous-semis n'a pas concurrencé le maïs. L'étude porte ensuite sur l'efficacité de la technique comme moyen de lutte antipollution. Les différents profils de sol prélevés ont clairement montré l'effet d'un sous-semis, effet beaucoup plus marqué sur un limon sableux que sur un sable limoneux. [less ▲]

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See detailFumure azotée des céréales: L'azote minéral du sol en 1997-1998: conseil de fumure pour 1998
Frankinet, Marc; Goffart, Jean-Pierre; Destain, Jean-Pierre ULg et al

in Fumure et protection phytosanitaire des céréales (1998, February)

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See detailFumure azotée et complémentation : Deux manières d'augmenter la charge des pâturages par la vache laitière
Limbourg, Pierre; Noirfalise, André; Bienfait, Jean-Marie ULg et al

in Annales de Médecine Vétérinaire (1980), 124

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See detailFumure azotée et protection fongicide des céréales d'hiver en Belgique
Bodson, Bernard ULg; Meeus, Patrick; Falisse, André

in Quatrième conférence internationale sur les maladies des plantes (1994, December 06)

The determination and the splitting of winter cereals nitrogen fertilisation as proposed and used in Belgium include three applications (tillering, beginning of stem elongation, flag leaf). While ... [more ▼]

The determination and the splitting of winter cereals nitrogen fertilisation as proposed and used in Belgium include three applications (tillering, beginning of stem elongation, flag leaf). While permitting to improve yield and haven quality, this largely contribute to limit croptogamic diseases development and thus the number of fungicide treatments needed to achieve an economic optimum. [less ▲]

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See detailLa fumure azotée
Destain, Jean-Pierre ULg; Couvreur, Luc; Herman, Jean-Luc et al

in Livre Blanc: Céréales - Gembloux (2007, February 28)

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See detailLa fumure azotée
Destain, Jean-Pierre ULg; Couvreur, Luc; Herman, Jean-Luc et al

Conference given outside the academic context (2008)

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See detailLa fumure azotée
Destain, Jean-Pierre ULg; Couvreur, Luc; Herman, Jean-Luc et al

in Livre Blanc: Céréales - Gembloux (2009, February 18)

Bilan de l'année écoulée et recommandations pratiques

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See detailLa fumure azotée
Destain, Jean-Pierre ULg; Roisin, Christian; Marcoen, Jean Marie ULg et al

in Livre Blanc: Céréales - Gembloux (2010, February 24)

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See detailFumure et protection phytosanitaire des céréales
Monfort, Bruno; Herman, Jean-Luc; Bodson, Bernard ULg et al

in Livre Blanc - Céréales Gembloux (2003, September 09)

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See detailFunción de la intertextualidad bíblica en "El retrato de la Lozana Andaluza" de Francisco Delgado
François, Jéromine ULg

Conference (2012, November 09)

La Lozana andaluza describes the story of a gifted prostitute which adventures occur in Rome before the Sack of 1527. However, the writer, an Andalusian priest, fills his text with biblical references. As ... [more ▼]

La Lozana andaluza describes the story of a gifted prostitute which adventures occur in Rome before the Sack of 1527. However, the writer, an Andalusian priest, fills his text with biblical references. As the purpose of his Portrait gives rise to debate and is valued according to its (un)appropriateness to the Christian doctrine, we study the role of the biblical intertextuality in the determination of this purpose. Indeed, in La Lozana, the biblical hypotext presents its traditional function as an auctoritas but it also constitutes a favored tool of linguistic games, a source of comic effects, and supplies the framework of the Portrait. In this work we examine the mixed intertextuality used by an autor which anticlericalism was sometimes interpreted as an erasmist feature. Consequently, we highlight, always making links with the biblical marks of La Lozana, first some evidences that make an orthodox interpretation possible, and later some dissident tendencies that cross the story. The biblical intertextuality produces a duplicity of the discourse that prevents an univocal interpretation. In fact, this posture is understandable during a time which is disturbed by religious tensions. [less ▲]

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See detailFunction and application of plant volatiles in ecological regulation and management of pest.
Zhou, Haibo ULg

(2008, November 02)

Study on the ecological regulation of pests was one of the hotspot in the researches of sustainable agriculture in recent years,and the plant volatiles have been shown to exert important influence on ... [more ▼]

Study on the ecological regulation of pests was one of the hotspot in the researches of sustainable agriculture in recent years,and the plant volatiles have been shown to exert important influence on behaviors of pests. This paper reviewed the effect and mechanism of plant volatiles to pests in host selection, attraction,antifeeding, repellence, growth inhibition, the prospect and application of plant volatiles to agricultural cultivation was further discussed. [less ▲]

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See detailFunction and dysfunction of the lower airways
Lekeux, Pierre ULg; Art, Tatiana ULg

in Proceedings: 9e Congrès de médecine et chirurgie équine (2005)

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See detailTHE FUNCTION OF HMG-BOX TRANSCRIPTION FACTORS Sox4a AND Sox4b IN ZEBRAFISH BONE DEVELOPMENT AND HOMEOSTASIS.
Aceto, Jessica ULg; Motte, Patrick ULg; Martial, Joseph ULg et al

in Journal of Gravitational Physiology : A Journal of the International Society for Gravitational Physiology (2008), 15

In mammals, the Sox4 gene is involved in development of endocardial crests, the brain, the lung, teeth, gonads and lymphocytes. Recently, Sox4 was shown to control bone mass and mineralization in mice. In ... [more ▼]

In mammals, the Sox4 gene is involved in development of endocardial crests, the brain, the lung, teeth, gonads and lymphocytes. Recently, Sox4 was shown to control bone mass and mineralization in mice. In zebrafish, two homologs for the mammalian Sox4 are present, sox4a and sox4b. Here we investigate the function of the sox4a and sox4b genes in cartilage and bone development in zebrafish. Therefore, we focus our attention on the first bone structures to be formed, the head skeleton and more precisely the pharyngeal cartilage. We show that both genes are expressed in the pharyngeal region, albeit at different time points during development. Double in situ hybridization experiments are used to exactly define the particular tissues where they are expressed. Furthermore, microinjection experiments of antisense oligonucleotides are used to block translation of these specific genes and to define their precise function during cartilage and bone development. [less ▲]

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See detailFunction of Penicillin-binding protein 3 in Streptococcus Faecium
Coyette, Jacques; Somzé, Anne; Briquet, Jean-Jacques et al

in Hakenbeck, Regine; Höltje, Joachim-Volker; Labischinski, Harald (Eds.) The Target Penicillin : the Murein Sacculus of Bacterial Cell Walls Architecture and Growth : Proceedings (1983)

Cefotaxime at concns. around the min. inhibitory concn. (MIC, 5 μM) or below (0.1-1.0 μM) causes transformation of the normal S. faecium cells to bacilliform cells whose length increases with increasing ... [more ▼]

Cefotaxime at concns. around the min. inhibitory concn. (MIC, 5 μM) or below (0.1-1.0 μM) causes transformation of the normal S. faecium cells to bacilliform cells whose length increases with increasing duration of treatment. Septa are initiated but never reach completion. Affinity detn. for cefotaxime binding showed that the antibiotic binds preferentially to the 3 highest mol. wt. penicillin-binding proteins (PBP); PBP-2 and PBP-3 are about 100-fold more sensitive to cefotaxime than PBP-1. It appears, therefore, that PbP-2 and PBP-3 are involved in cell septation. This was confirmed by using cefatoxime in subinhibitory concns. (1 μM). From cell samples collected 30 and 60 min after addn. of cefotaxime, membranes were isolated, labeled with satg. [3H]benzylpenicillin and examd. by fluorog. Under these conditions only PBP-2 and PBP-3 were satd. by cefotaxime. At this stage no distinction could be made between the 2 proteins; either both or 1 of them may be involved in cell division. Cefoxitin produced morphol. alteration of a different nature than cefotaxime. The cefoxitin-treated cells had increased diam. and were slightly elongated. The most striking alteration was the frequent presence of conical poles contrasting with round poles obsd. in control cells. The morphol. alteration obsd. in cefoxitin-treated cells could be attributed to the inhibition of the function of PBP-1, PBP-2, or PBP-3. Elongated cells similar to those obtained with cefotaxime were not found with cefoxitin at concns. sufficient to sat. PBP-2. The main difference between cefotaxime- and cefoxitin-treated cells is that PBP-3 is satd. by cefotaxime but not altered at all by cefoxitin. Thus, septation inhibition must be due to the interaction of cefotaxime with PBP-3 [less ▲]

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See detailFunction of the Chloroplast Hydrogenase in the Microalga Chlamydomonas: The Role of Hydrogenase and State Transitions during Photosynthetic Activation in Anaerobiosis
Ghysels, Bart ULg; Godaux, Damien ULg; Matagne, René-Fernand ULg et al

in PLoS ONE (2013), 8(5), 64161

Like a majority of photosynthetic microorganisms, the green unicellular alga Chlamydomonas reinhardtii may encounter O2 deprived conditions on a regular basis. In response to anaerobiosis or in a ... [more ▼]

Like a majority of photosynthetic microorganisms, the green unicellular alga Chlamydomonas reinhardtii may encounter O2 deprived conditions on a regular basis. In response to anaerobiosis or in a respiration defective context, the photosynthetic electron transport chain of Chlamydomonas is remodeled by a state transition process to a conformation that favours the photoproduction of ATP at the expense of reductant synthesis. In some unicellular green algae including Chlamydomonas, anoxia also triggers the induction of a chloroplast-located, oxygen sensitive hydrogenase, which accepts electrons from reduced ferredoxin to convert protons into molecular hydrogen. Although microalgal hydrogen evolution has received much interest for its biotechnological potential, its physiological role remains unclear. By using specific Chlamydomonas mutants, we demonstrate that the state transition ability and the hydrogenase function are both critical for induction of photosynthesis in anoxia. These two processes are thus important for survival of the cells when they are transiently placed in an anaerobic environment. [less ▲]

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