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See detailFurther heterogeneity demonstarted for serum creatine kinase isoenzyme MM
Chapelle, Jean-Paul ULg; Heusghem, C.

in Clinical Chemistry (1980), 26(3), 457-62

Serum creatine kinase (EC 2.1.3.2) isoenzyme MM was resolved by isoelectric focusing into a five-band pattern, a pattern that gradually changed after the onset of myocardial infarction. Similar changes ... [more ▼]

Serum creatine kinase (EC 2.1.3.2) isoenzyme MM was resolved by isoelectric focusing into a five-band pattern, a pattern that gradually changed after the onset of myocardial infarction. Similar changes were also demonstrated in patients undergoing coronary-bypass surgery. The evolution of two CK-MB sub-bands was studied in both cases. We found that three electrophoretic bands (CK-MM, pI 7.10; MM1, pI 6.88; MB1, pI 5.61) were predominant in patterns for sera collected during the early phase of myocardial infarction, but rapidly disappeared during the following hours, whereas bands of increased electrophoretic mobility (MM2, pI 6.70; MM3, pI 6.45; MM4, pI 6.25; MB2, pI 5.34) gradually increased. MM3 was always the major band at the end of the observation period in acute myocardial infarction (mean, 61.4% of total creatine kinase activity 36 h after the peak value for total creatine kinase in serum). The CK-MM bands were also present in the serum of patients without heart disease. Changes in the electrophoretic pattern were induced by a thermolabile factor in normal human serum, which transformed the muscular or myocardial MM and MM1 bands after their release into the blood stream [less ▲]

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See detailFurther insight into the sound-producing mechanism of clownfishes: what structure is involved in sound radiation?
Colleye, Orphal ULg; Nakamura, Masaru; Frederich, Bruno ULg et al

in Journal of Experimental Biology (2012), 215

It was recently demonstrated that clownfishes produce aggressive sounds by snapping their jaw teeth. To date, only the onset of the sound has been studied, which raises the question, what structure is ... [more ▼]

It was recently demonstrated that clownfishes produce aggressive sounds by snapping their jaw teeth. To date, only the onset of the sound has been studied, which raises the question, what structure is involved in sound radiation? Here, a combination of different approaches has been used to determine the anatomical structure(s) responsible for the size-related variations observed in sound duration and frequency. Filling the swimbladder with physiological liquid specifically modified size-related acoustic features by inducing a significant decrease in pulse duration of approximately 3 ms and a significant increase in dominant frequency of approximately 105 Hz. However, testing the acoustics of the swimbladder by striking it with a piezoelectric impact hammer showed that this structure is a highly damped sound source prevented from prolonged vibrations. In contrast, the resonant properties of the rib cage seems to account for the size-related variations observed in acoustic features. For an equivalent strike on the rib cage, the duration and dominant frequency of induced sounds changed with fish size: sound duration and dominant frequency were positively and negatively correlated with fish size, respectively. Such relationships between sonic features and fish size are consistent with those observed in natural sounds emitted by fish. Therefore, the swimbladder itself does not act as a resonator; its wall just seems to be driven by the oscillations of the rib cage. This set of observations suggests the need for reassessment of the acoustic role of swimbladders in various fish species. [less ▲]

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See detailFurther insight on carapid - holothuroid relationships
Parmentier, Eric ULg; Vandewalle, Pierre ULg

in Marine Biology (2005), 146(3), 455-465

Carapidae (or pearlfish) are eel-like fishes that live inside different invertebrates, such as holothurians, sea stars, or bivalves. Those of the genus Carapus are commensal and use their host as a ... [more ▼]

Carapidae (or pearlfish) are eel-like fishes that live inside different invertebrates, such as holothurians, sea stars, or bivalves. Those of the genus Carapus are commensal and use their host as a shelter, while Encheliophis species are parasitic and eat the host's gonads. In areas where they live in sympatry, C. boraborensis, C. homei, C. mourlani and E. gracilis are able to inhabit the same host species. Infestation is considered as monospecific when several conspecifics are observed in the same host. However, many aspects of this particular relation remain obscure, e.g. communication between carapids and the defence systems of the different protagonists (carapids and hosts). Experiments have been conducted in the field and laboratory to investigate several aspects of the carapids' relationships with their hosts. Sampling carried out in the Bay of Opunohu (Moorea, French Polynesia) determined the sex ratio of C. boraborensis (3:1) and C. homei (1:1) and their distribution rate within different Echinodermata. Our study showed that neither species was capable of determining whether a heterospecific already occupied a sea cucumber or not. They were, however, able to locate the sea cucumber's cloaca, due to the excurrent resulting from respiration. The sea cucumber's defence system (Cuverian tubules) minimises predator attacks, but is not effective against carapid intrusion. The Carapidae defence system is twofold. Due to a passive system related to the sea cucumber's low cloacal position, the Cuverian tubules are not expelled when fish enter the cloaca. Moreover, carapids resist sea cucumber toxins better than other reef fish. Their increased resistance might be related to their gills rather than to their mucus coating; however, the latter may assist the fish in resisting the sticky substances emitted by the Cuverian tubules. [less ▲]

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See detailFurther insights in the mechanisms of interleukin-1beta stimulation of osteoprotegerin in osteoblast-like cells
Lambert, Cécile ULg; Oury, Cécile ULg; Dejardin, Emmanuel ULg et al

in Journal of Bone and Mineral Research (2007), 22(9), 1350-1361

The mechanisms of IL-1beta stimulation of OPG were studied in more detail. Whereas p38 and ERK activation was confirmed to be needed, NF-kappaB was not necessary for this regulation. We also found that ... [more ▼]

The mechanisms of IL-1beta stimulation of OPG were studied in more detail. Whereas p38 and ERK activation was confirmed to be needed, NF-kappaB was not necessary for this regulation. We also found that OPG production after IL-1beta stimulation was not sufficient to block TRAIL-induced apoptosis in MG-63 cells. INTRODUCTION: Osteoprotegerin (OPG) plays a key role in the regulation of bone resorption and is stimulated by interleukin (IL)-1beta. Herein, we defined the mechanisms of IL-1beta stimulation of OPG focusing on the potential involvement of MAPK and NF-kappaB. We also examined whether OPG production in response to IL-1beta influences TRAIL-induced apoptosis in MG-63 cells. MATERIALS AND METHODS: OPG mRNA levels in MG-63 cells were quantified by real-time RT-PCR and protein levels of OPG and IL-6 by ELISA. Cell viability was assessed using the methyltetrazidium salt (MTS) reduction assay. The role of the MAPK pathway was studied by both Western blotting and the use of specific chemical inhibitors. NF-kappaB function was studied using BAY 11-7085 and by siRNA transfection to inhibit p65 synthesis. Transcription mechanisms were analyzed by transiently transfecting MG-63 cells with OPG promoter constructs. Post-transcriptional effects were examined by using cycloheximide and actinomycin D. RESULTS: MG-63 cells treatment with IL-1beta resulted in the phosphorylation of c-Jun NH(2)-terminal kinase (JNK), p38, and extracellular signal-regulated kinase (ERK). The use of the specific inhibitors showed that p38 and ERK but not JNK were needed for IL-1beta-induced OPG production. In contrast, NF-kappaB was not essential for IL-1beta induction of OPG. We also showed a small transcriptional and a possible post-transcriptional or translational regulation of OPG by IL-1beta. Exogenous OPG blocked TRAIL-induced apoptosis, but IL-1beta induction of OPG did not influence TRAIL-induced cell death. CONCLUSIONS: IL-1beta stimulates OPG production by mechanisms dependent on p38 and ERK. In contrast, NF-kappaB was not essential for this regulation. Although the relevance of IL-1beta stimulation of OPG is still not fully understood, our data showed that IL-1beta stimulation of OPG does not modify TRAIL-induced cell death. [less ▲]

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See detailFurther investigation of the pair of quasars Q0107 - 025 A and B
Surdej, Jean ULg; Arp, H.; Gosset, Eric ULg et al

in Astronomy and Astrophysics (1986), 161

Low and intermediate dispersion spectroscopy of Q0107 - 025 A and B obtained at ESO, AAT, and MMT provide evidence for spectral differences between these two quasars. A redshift difference of 0.004 is ... [more ▼]

Low and intermediate dispersion spectroscopy of Q0107 - 025 A and B obtained at ESO, AAT, and MMT provide evidence for spectral differences between these two quasars. A redshift difference of 0.004 is found between the two objects, and forbidden emission lines due to O II (3727 A), Ne III (1815), and possibly Ne V (3426 A), are seen in the spectrum of Q0107 - 025 A, while only the forbidden Ne III line at 1815 A is possibly detected in the spectdrum of Q0107 - 025 B. No narrow absorption lines could be detected in the 3252 - 5616 A spectral range of the intermediate resolution data of either quasar, and the spectrum of quasar B is found to be steeper in the blue that of the quasar A. The possible presence of a highly redshifted cluster of galaxies in the field of these quasars is suggested by CCD photometry. [less ▲]

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See detailFurther investigation of the supervisory attentional system in schizophrenia: Planning, inhibition, and rule abstraction
Marczewski, P.; Van der Linden, Martial ULg; Laroi, Frank ULg

in Cognitive Neuropsychiatry (2001), 6(3), 175-192

Investigated supervisory attentional system (SAS) functions in schizophrenic patients. 15 schizophrenic outpatients (mean age 29.93 yrs) and 15 normal controls matched for age, gender, and educational ... [more ▼]

Investigated supervisory attentional system (SAS) functions in schizophrenic patients. 15 schizophrenic outpatients (mean age 29.93 yrs) and 15 normal controls matched for age, gender, and educational level were compared. Three tasks (Tower of London, Hayling, and Brixton tests) were used to examine 3 executive processes (planning, inhibition, and rule detection). Schizophrenic patients performed significantly worse than normal controls on all 3 tasks. In addition, patients' performances on selected measures of the three tests were correlated, even when age and medication were partialled out. It is concluded that patients are impaired on all SAS functions. The possible role of a general impairment (processing speed or cognitive resources) is discussed [less ▲]

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See detailA further new species in the lichen genus Arctomia: A. borbonica from Reunion (Mascarene archipelago)
Magain, Nicolas ULg; Sérusiaux, Emmanuël ULg

in MycoKeys (2012), 4

Arctomia borbonica sp. nov. is described as new for science from montane natural and secondary habitats in Reunion in the Mascarene archipelago (Indian Ocean). It has a sterile, foliose, usually wrinkled ... [more ▼]

Arctomia borbonica sp. nov. is described as new for science from montane natural and secondary habitats in Reunion in the Mascarene archipelago (Indian Ocean). It has a sterile, foliose, usually wrinkled, thallus whose margins produce goniocysts that disintegrate into a soredioid margin; it looks like a Leptogium species. Its phylogenetic position in the Arctomiaceae (Ostropomycetidae, Ascomycota) has been determined with 3 genes (nuLSU, mtSSU, RPB1) inferences. [less ▲]

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See detailFurther observational evidence that MG J0414+0534 is a gravitational mirage
Angonin-Willaime, M.-C.; Vanderriest, C.; Hammer, F. et al

in Astronomy and Astrophysics (1994), 281

Deep imaging of MG J0414+0534 with R and I filters reveals a faint, fuzzy and red object at the exact location expected for a lensing galaxy in the gravitational mirage hypothesis. Furthermore, the ... [more ▼]

Deep imaging of MG J0414+0534 with R and I filters reveals a faint, fuzzy and red object at the exact location expected for a lensing galaxy in the gravitational mirage hypothesis. Furthermore, the (extremely red and almost featureless) spectra of the 2 brightest components are very similar. These are strong indications that the system results from multiple gravitational imaging of a single source, but the nature of this source is not yet clear. It could be the nucleus of a low metallicity galaxy at a high redshift or a new type of object. Significant differences are observed between the flux ratios of the images at radio and optical wavelenghts. The most likely explanation for this effect is a differential amplification of the image pair A[SUB]1[/SUB]-A[SUB]2[/SUB] because of the large magnification gradient near a caustic. [less ▲]

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See detailFurther observations of the light echoes from SN 1987 A
Detal, Alain ULg; Surdej, Jean ULg; Hutsemekers, Damien ULg et al

in Astronomy and Astrophysics (1990), 229

Further direct observations of the light echoes from SN 1987 A were obtained on November 12 1988 with the 3.6-m telescope and EFOSC at ESO. In agreement with predictions made from earlier observations ... [more ▼]

Further direct observations of the light echoes from SN 1987 A were obtained on November 12 1988 with the 3.6-m telescope and EFOSC at ESO. In agreement with predictions made from earlier observations, the radii of the two light rings are measured to be 43.3 and 72.5 arcsec. However, a noticeable diaplacement of the center of the outer ring by 2.6 arcsec to the NE from the SN position is also reported. Application of the model of planar-like sheets of material located between the SN and the observer leads to improved results. [less ▲]

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See detailFurther pharmacological and genetic evidence for the efficacy of PlGF inhibition in cancer and eye disease.
Van de Veire, Sara; Stalmans, Ingeborg; Heindryckx, Femke et al

in Cell (2010), 141(1), 178-90

Our findings that PlGF is a cancer target and anti-PlGF is useful for anticancer treatment have been challenged by Bais et al. Here we take advantage of carcinogen-induced and transgenic tumor models as ... [more ▼]

Our findings that PlGF is a cancer target and anti-PlGF is useful for anticancer treatment have been challenged by Bais et al. Here we take advantage of carcinogen-induced and transgenic tumor models as well as ocular neovascularization to report further evidence in support of our original findings of PlGF as a promising target for anticancer therapies. We present evidence for the efficacy of additional anti-PlGF antibodies and their ability to phenocopy genetic deficiency or silencing of PlGF in cancer and ocular disease but also show that not all anti-PlGF antibodies are effective. We also provide additional evidence for the specificity of our anti-PlGF antibody and experiments to suggest that anti-PlGF treatment will not be effective for all tumors and why. Further, we show that PlGF blockage inhibits vessel abnormalization rather than density in certain tumors while enhancing VEGF-targeted inhibition in ocular disease. Our findings warrant further testing of anti-PlGF therapies. [less ▲]

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See detailFurther photomorphs in the lichen family Lobariaceae from Reunion (Mascarene archipelago) with notes on the phylogeny of Dendriscocaulon cyanomorphs
Magain, Nicolas ULg; Goffinet, Bernard; Sérusiaux, Emmanuël ULg

in Bryologist (2012), 115(2), 243-254

Two new photomorphs in the Lobariaceae have been found on the remote island of Reunion in the Mascarene archipelago: the free-living Dendriscocaulon-like cyanomorph of the pantropical Lobaria discolor ... [more ▼]

Two new photomorphs in the Lobariaceae have been found on the remote island of Reunion in the Mascarene archipelago: the free-living Dendriscocaulon-like cyanomorph of the pantropical Lobaria discolor, and the cyanomorph of Sticta dichotoma, a species apparently endemic to the western parts of the Indian Ocean, known only from its chloromorph. Inferences from three loci demonstrate that the fungus involved in each morph of either pair belongs to the same species. Phylogenetic analyses resolve all genera of the Lobariaceae as polyphyletic, and all Dendriscocaulon-like cyanomorphs within Lobaria, except for D. dendroides, which belongs to Sticta. [less ▲]

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See detailFurther Purification and Characterization of the DNA 3'-Phosphatase from Rat-Liver Chromatin Which Is Also a Polynucleotide 5'-Hydroxyl Kinase
Habraken, Yvette ULg; Verly, W. G.

in European Journal of Biochemistry (1988), 171(1-2), 59-66

The DNA 3'-phosphatase activity of rat-liver chromatin has been purified. A DNA 5'-hydroxyl kinase activity comigrates at each step of purification. Both enzymes have the same molecular mass (79 kDa) and ... [more ▼]

The DNA 3'-phosphatase activity of rat-liver chromatin has been purified. A DNA 5'-hydroxyl kinase activity comigrates at each step of purification. Both enzymes have the same molecular mass (79 kDa) and the same isoelectric point (8.6). It thus seems that the two activities are born by the same protein just as with the phage T4 enzyme which is, at the same time, a 5'-hydroxyl kinase and a 3'-phosphatase. An additional argument is that ATP, which does not influence the rate of the 3'-phosphatase reaction but which is a cosubstrate of the 5'-hydroxyl kinase, protects the 3'-phosphatase activity against thermal denaturation and trypsin digestion. The two active sites must, however, be largely independent within a common support: the thermal denaturation and trypsin inactivation rates are very different for the two activities; increasing the ionic strength activates the kinase and inhibits the phosphatase; polyvalent anions inhibit the phosphatase and have little effect on the kinase. The two active sites might belong to different domains of the protein; they could not however be separated by a partial trypsin digestion. The rates of 3'-dephosphorylation and 5'-phosphorylation by the chromatin enzyme are the same in native and denatured DNA. The 3'-phosphatase has no action on 3'-monodeoxynucleotide, but it hydrolyzes the 3'-phosphate in dinucleotides. The Km of the 3'-phosphatase is 0.548 microM. The Km (5'-OH) and Km (ATP) of the 5'-hydroxyl kinase are about 3.9 microM and 0.69 microM respectively. The chromatin enzyme is unable to hydrolyze 3'-phosphoglycolate ends in DNA. [less ▲]

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See detailFurther reduction in nonvertebral fracture rate is observed following 3 years of denosumab treatment: results with up to 7 years in the freedom extension
Ferrari, S; Adachi, JD; Lippuner, K et al

in Osteoporosis International (2014), 25(2), 56

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See detailFurther results on the line-profile variability of WR 134
Gosset, Eric ULg; Vreux, Jean-Marie ULg; Andrillat, Y.

in Astrophysics & Space Science (1994, November 01), 221

We discuss a few conclusions previously derived from the analysis of the line-profile variability of the Wolf-Rayet star WR 134, some details are made more explicit. In addition, we present a new set of ... [more ▼]

We discuss a few conclusions previously derived from the analysis of the line-profile variability of the Wolf-Rayet star WR 134, some details are made more explicit. In addition, we present a new set of data and briefly comment on recent results deduced from its analysis. [less ▲]

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See detailFurther spectroscopic observations of 25 quasi-stellar objects
Surdej, Jean ULg; Swings, Jean-Pierre ULg

in Astronomy and Astrophysics. Supplement Series (1981), 46

Spectroscopic observations are reported for 25 quasars suspected of having line profiles which are indicative of mass outflow from the central quasar. The quasars were selected on the basis of optical ... [more ▼]

Spectroscopic observations are reported for 25 quasars suspected of having line profiles which are indicative of mass outflow from the central quasar. The quasars were selected on the basis of optical variability and/or the presence of P Cygni spectral profiles, and large emission redshift, and observed on widened spectrograms obtained at the Cassegrain focus of the ESO 3.6-m telescope at La Silla during December 1977 and July 1978. Observations confirm the previously detected emission and absorption redshifts for most of the quasars, and reveal previously undetected lines and absorption redshifts in a few cases. [less ▲]

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See detailFurther studies on the genetic diversity of lima beans (Phaseolus lunatus L.) using allozymes
Maquet, Alain; Wathelet, Bernard ULg; Baudoin, Jean-Pierre ULg

in Annual Report of the Bean Improvment Cooperative (1993), 36

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See detailFurther Studies on the Mechanism of Radiation Induced Thymic Lymphoma Prevention by Bone Marrow Transplantation in C57bl Mice
Humblet, Chantal ULg; Defresne, Marie-Paule ULg; Greimers, Roland ULg et al

in Leukemia : Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K (1989), 3(11), 813-8

Whole body fractionated irradiation induces thymic lymphomas in C57BL/Ka mice after a latent period during which intrathymic lymphopoiesis is modified; thymocyte numbers are subnormal and the epithelial ... [more ▼]

Whole body fractionated irradiation induces thymic lymphomas in C57BL/Ka mice after a latent period during which intrathymic lymphopoiesis is modified; thymocyte numbers are subnormal and the epithelial component of thymic nurse cells (TNCs) is altered as estimated by the number of TNCs in vivo and by its ability to interact with immature thymocytes in vitro. A graft of normal bone marrow cells immediately after the last irradiation prevents the development of lymphomas; but when such a graft is performed 1 month later, it does not inhibit the emergence of tumors. In both cases the grafted precursors home and repopulate the thymus. However, the delayed graft does not exert any effect upon the altered epithelial component of TNCs, whereas the early one restores the numbers of TNCs and the function of their epithelial component. The results thus demonstrate that lymphoid thymic repopulation by a bone marrow graft is not sufficient to prevent the development of lymphomas and that there is an intimate relationship between tumor development and alterations of nurse cells microenvironment. [less ▲]

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See detailFurther taxonomic rearrangements in the Brachytheciaceae (Bryophyta): Frahmiella, a new genus segregated from Rhynchostegiella
Ignatov, Michael; Aigoin, Delphine ULg; Huttunen, Sanna et al

in Tropical Bryology (2010), 31

The taxonomic position of Rhynchostegiella acicula, a local endemic of Shaanxi Province of China, is investigated by means of cladistic analyses employing nrITS sequences. The analyses show that R ... [more ▼]

The taxonomic position of Rhynchostegiella acicula, a local endemic of Shaanxi Province of China, is investigated by means of cladistic analyses employing nrITS sequences. The analyses show that R. acicula does not belong to Rhynchostegiella s.str. (Helicodontioideae) but is resolved within the Homalothecioideae as sister to Eurhynchiastrum, from which it differs by a soft and slender habit; narrow lanceolate and acuminate leaves; a percurrent costa; and an autoicous condition. It differs from Brachytheciastrum and Brachythecium in a longly rostrate operculum, and from Homalothecium in a small plants that lack thick-walled basal laminal cells characteristic of this genus, as well as in an autoicous inflorescence. As a consequence, R. acicula is transferred into its own, monospecific genus Frahmiella Ignatov, Vanderpoorten & Wang You-fang, gen. nov. [less ▲]

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See detailFusariose disséminée chez un patient immunodéprimé
QUATRESOOZ, Pascale ULg; Meiers, I.; ARRESE ESTRADA, Jorge ULg et al

in Revue de l’Association Belge des Technologues de Laboratoire = Tijdschrift van de Belgische Vereniging van Laboratoriumtechnologen (2001), 28

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See detailFusarioses sur blé d'hiver au Grand-Duché de Luxembourg
Giraud, Frédéric; Pasquali, Matias; Vrancken, Carine et al

in Phytoma - la Défense des Végétaux (2009), 622-623

Au Grand-Duché de Luxembourg, la campagne 2006-2007 a été caractérisée par un hiver relativement doux et un printemps pluvieux. Ces conditions météorologiques ont permis le développement de nombreuses ... [more ▼]

Au Grand-Duché de Luxembourg, la campagne 2006-2007 a été caractérisée par un hiver relativement doux et un printemps pluvieux. Ces conditions météorologiques ont permis le développement de nombreuses maladies cryptogamiques sur le blé d’hiver, dont la fusariose. Cette maladie a été suivie sur 17 sites couvrant le G-D de Luxembourg par des mesures de prévalence (pourcentage moyen d’épis visuellement infectés) et de sévérité (pourcentage moyen d’épillets atteints par épi). Des valeurs moyennes de 8,9± +/- 15,5 % pour la prévalence et de 21,0± +/- 17,8 % pour la sévérité ont été enregistrées avec des différences significatives entre les cantons nord et sud pour la sévérité. Plus de 600 souches isolées des 17 sites ont été identifiées suivant des critères morphologiques et l’emploi d’outils moléculaires. 5 espèces semblent associées à la fusariose : Fusarium graminearum (23,5 %), F. culmorum (12,8 %), F. poae (19,4 %) (3 espèces potentiellement productrices de mycotoxines), F. avenaceum (20 %) et Microdochium nivale (18,7 %). Cette étude est la première du genre à avoir été faite au Grand-Duché de Luxembourg. [less ▲]

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