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See detailFunctional response to inhaled salbutamol and/or ipratropium bromide in Ascaris suum-sensitised cats with allergen-induced bronchospasms
Leemans, Jérôme; Kirschvink, N.; Clercx, Cécile ULg et al

in Veterinary Journal (2010)

Knowledge about the use of inhaled bronchodilators in cats with so-called 'feline asthma' is limited and relies on the experience of clinicians treating these patients. A randomised controlled four-way ... [more ▼]

Knowledge about the use of inhaled bronchodilators in cats with so-called 'feline asthma' is limited and relies on the experience of clinicians treating these patients. A randomised controlled four-way crossover study was therefore designed to compare the effects of salbutamol (SAL, 100 μg), ipratropium bromide (IB, 20 μg) and a combination of both (SAL/IB, 100 μg/20 μg), delivered through a pressurised metered-dose inhaler (pMDI) connected to a spacing chamber, on allergen-induced bronchospasms in five Ascaris suum (AS)-sensitised cats. Four AS bronchial provocation challenges were carried out at 1 week intervals, followed by one of four treatment protocols: SAL, IB, SAL/IB or control (untreated). Enhanced pause (Penh), an estimator of airflow limitation measured by barometric whole-body plethysmography, was repeatedly assessed within 120 min following the administration of each treatment protocol. Responses to inhaled medications were evaluated by calculating the area under the time-response curves (AUC) from 0 to 60 or 120 min after drug administration (AUC(0-60), AUC(0-120)), as well as the times required for half-recovery (T(50%)) or for returning to nearly basal conditions (T(20%)). No significant differences were found among the four study groups, with reference to the mean AUC(0-60), T(20%) and T(50%) values of Penh (P>0.05). Mean AUC(0-120) values of Penh were similar between the bronchodilators tested, but were significantly lower than those in the untreated group. It was concluded that inhalation of SAL, IB and SAL/IB via pMDI failed to improve most Penh-derived parameters, which suggested that these bronchodilators were of limited efficacy in reversing allergen-induced bronchospasm in cats. However, further studies using a larger number of animals are warranted to investigate if different drugs or delivery devices or higher dosages may be more effective [less ▲]

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See detailFunctional screening of a metagenomic library from algal biofilms
Martin, Marjolaine ULg

Conference (2013, February 08)

Macroalgae, and particularly their lignin-free polysaccharides, are increasingly used for their gelling and therapeutic properties and for the production of biofuels and renewable chemical compounds. To ... [more ▼]

Macroalgae, and particularly their lignin-free polysaccharides, are increasingly used for their gelling and therapeutic properties and for the production of biofuels and renewable chemical compounds. To extract, hydrolyze and purify this biomass, algae hydrolyzing enzymes are needed. Our work aims to identify and characterize algal biomass hydrolyzing enzymes expressed by microorganisms living on the surface of algae, by functional metagenomics. Therefore, a microbial DNA extraction method was developed to isolate the gDNA from the microorganisms of the brown algae Ascophyllum nodosum and a metagenomic library was constructed in Escherichia coli. The library was screened for diverse enzymatic activities (esterases, xylanases, cellulases, α-amylases, arabinanases, caseinases and β-glucosidases) on agar plates with specific enzymes substrates. Several new microbial enzymes (esterases, β-glucosidases, α-amylases and cellulases) were identified revealing the wealth of our library. Furthermore, those enzymes had less than 50% sequence identity with known protein sequences; meaning that our approach allows to identify new microbial enzymes expressed by uncultured microorganisms. Plate tests for medium-throughput screening of specific enzymes hydrolyzing algal polysaccharides (agarases, carrageenases and alginate lyases) are currently being developed. Our approach will probably allow us to identify new families of those ill-known enzymes, with particular enzymatic activities. [less ▲]

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See detailFunctional screening of a metagenomic library from algal biofilms
Martin, Marjolaine ULg; Barbeyron, Tristan; Michel, Gurvan et al

in Smagghe, Guy; Boeckx, Pascal; Bossier, Peter (Eds.) et al Communications in Agricultural and Applied Biological Sciences (2013, February 08)

Macroalgae, and particularly their lignin-free polysaccharides, are increasingly used for their gelling and therapeutic properties and for the production of biofuels and renewable chemical compounds. To ... [more ▼]

Macroalgae, and particularly their lignin-free polysaccharides, are increasingly used for their gelling and therapeutic properties and for the production of biofuels and renewable chemical compounds. To extract, hydrolyze and purify this biomass, algae hydrolyzing enzymes are needed. Our work aims to identify and characterize algal biomass hydrolyzing enzymes expressed by microorganisms living on the surface of algae, by functional metagenomics. Therefore, a microbial DNA extraction method was developed to isolate the gDNA from the microorganisms of the brown algae Ascophyllum nodosum and a metagenomic library was constructed in Escherichia coli. The library was screened for diverse enzymatic activities (esterases, xylanases, cellulases, α-amylases, arabinanases, caseinases and β-glucosidases) on agar plates with specific enzymes substrates. Several new microbial enzymes (esterases, β-glucosidases, α-amylases and cellulases) were identified revealing the wealth of our library. Furthermore, those enzymes had less than 50% sequence identity with known protein sequences; meaning that our approach allows to identify new microbial enzymes expressed by uncultured microorganisms. Plate tests for medium-throughput screening of specific enzymes hydrolyzing algal polysaccharides (agarases, carrageenases and alginate lyases) are currently being developed. Our approach will probably allow us to identify new families of those ill-known enzymes, with particular enzymatic activities. [less ▲]

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See detailFunctional screening of a metagenomic library of seaweed-associated microbiota: identification and characterization of a halotolerant, cold-active marine endo-ß-1,4-endoglucanase
Martin, Marjolaine ULg; Biver, Sophie ULg; Steels, Sébastien ULg et al

in Applied and Environmental Microbiology (2014)

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed thirteen novel putative esterase genes and two ... [more ▼]

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed thirteen novel putative esterase genes and two glycoside hydrolase genes. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection period. Lastly, an endo-ß-1,4-endoglucanase having less than 50% identity to sequences of known cellulases was purified and partially characterized, showing activity at low temperature and after prolonged incubation in concentrated salt solutions. [less ▲]

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See detailFunctional screening of a winter and a spring genomic DNA libraries obtained from soils in a winter wheat crop
Stroobants, Aurore ULg; Portetelle, Daniel ULg; Vandenbol, Micheline ULg

Poster (2013, June 10)

Soils are very rich environments where the diversity of microorganisms is very high. These microorganisms play important role in the degradation of organic matter with enzymes able to degrade it. The aim ... [more ▼]

Soils are very rich environments where the diversity of microorganisms is very high. These microorganisms play important role in the degradation of organic matter with enzymes able to degrade it. The aim of this work is to discover by functional screening new enzymatic activities of microorganisms from soils collected in winter and spring in a winter wheat crop. The genomic DNA was extracted from both soils to construct two libraries in Escherichia coli. These libraries were then screened for several enzymes such as lipase, beta-glucosidase, cellulase, α-amylase,… At this time, 2 beta-glucosidases and 3 lipases have already been found in the winter library and 3 beta-glucosidases and 1 lipase in the spring library. Sequence analyses with the BLASTX program revealed that two beta-glucosidases have less than 65% of sequence identity with known beta-glucosidases, one have 64% of identity with a known beta-galactosidase and one have 59% of identity with a glycoside hydrolase. The fifth seems to be a phosphorylase kinase (54% identity) which have a glucoamylase domain responsible for the activity. This ORF is interrupted by a transposase. Three of the four lipases have less than 60% of sequence identity with known lipases/esterases. The fourth show 55% of identity with a known beta-lactamase. [less ▲]

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See detailFunctional Shape Matching and Fuzzy Templates
Delanoy, Richard L.; Verly, Jacques ULg; Dudgeon, Dan E.

Conference (1991, September)

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See detailThe functional significance of E-b-Farnesene: Does it influence the populations of aphid natural enemies in the fields?
Cui, L-L; Francis, Frédéric ULg; Heuskin, Stéphanie ULg et al

in Biological Control (2012)

Aphids cause much damage to Chinese cabbage in northern China. Over reliance on pesticides have large environmental and human health costs that compel researchers to seek alternative management tactics ... [more ▼]

Aphids cause much damage to Chinese cabbage in northern China. Over reliance on pesticides have large environmental and human health costs that compel researchers to seek alternative management tactics for aphid control. The component of aphid alarm pheromone, E-b-Farnesene (EbF), extracted from Matricaria chamomilla L., which attracts natural enemies in the laboratory, may have significant implications for the design of cabbage aphid control strategies. The purpose of this paper is to understand the effects of EbF on natural enemies to cabbage aphid control in Chinese cabbage fields. Ladybeetles on Chinese cabbage leaves in EbF released plots and Aphidiidae in EbF released yellow traps were significantly higher than those of in controls. No significant differences were detected in the interactions of different treatments and the two years for all natural enemies. More important, lower aphid densities were found in EbF released plots. Our results suggested that the EbF extracted from M. chamomilla L. could attract natural enemies to reduce cabbage aphids in the Chinese cabbage fields. [less ▲]

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See detailFunctional significance of the rapid regulation of brain estrogen action: Where do the estrogens come from?
Cornil, Charlotte ULg; Ball, G. F.; Balthazart, Jacques ULg

in Brain Research (2006), 1126

Estrogens exert a wide variety of actions on reproductive and non-reproductive functions. These effects are mediated by slow and long lasting genomic as well as rapid and transient non-genomic mechanisms ... [more ▼]

Estrogens exert a wide variety of actions on reproductive and non-reproductive functions. These effects are mediated by slow and long lasting genomic as well as rapid and transient non-genomic mechanisms. Besides the host of studies demonstrating the role of genomic actions at the physiological and behavioral level, mounting evidence highlights the functional significance of non-genomic effects. However, the source of the rapid changes in estrogen availability that are necessary to sustain their fast actions is rarely questioned. For example, the rise of plasma estrogens at pro-estrus that represents one of the fastest documented changes in plasma estrogen concentration appears too slow to explain these actions. Alternatively, estrogen can be synthesized in the brain by the enzyme aromatase providing a source of locally high concentrations of the steroid. Furthermore, recent studies demonstrate that brain aromatase can be rapidly modulated by afferent inputs, including glutamatergic afferents. A role for rapid changes in estrogen production in the central nervous system is supported by experiments showing that acute aromatase inhibition affects nociception as well as male sexual behavior and that preoptic aromatase activity is rapidly (within min) modulated following mating. Such mechanisms thus fulfill the gap existing between the fast actions of estrogen and their mode of production and open new avenues for the understanding of estrogenic effects on the brain. (c) 2006 Elsevier B.V. All rights reserved. [less ▲]

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See detailFunctional Sites Of The Bovine Leukemia-Virus Envelope Glycoproteins Using Structural And Immunological Data
Callebaut, I.; Mornon, Jp.; Burny, A. et al

in Archives Internationales de Physiologie de Biochimie et de Biophysique (1994), 102(2),

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See detailFunctional specialization for auditory-spatial processing in the occipital cortex of congenitally blind humans
Collignon, Olivier; Vandewalle, Gilles ULg; Voss, Patrice et al

in Proceedings of the National Academy of Sciences of the United States of America (2011), 108(11), 4435-40

The study of the congenitally blind (CB) represents a unique opportunity to explore experience-dependant plasticity in a sensory region deprived of its natural inputs since birth. Although several studies ... [more ▼]

The study of the congenitally blind (CB) represents a unique opportunity to explore experience-dependant plasticity in a sensory region deprived of its natural inputs since birth. Although several studies have shown occipital regions of CB to be involved in nonvisual processing, whether the functional organization of the visual cortex observed in sighted individuals (SI) is maintained in the rewired occipital regions of the blind has only been recently investigated. In the present functional MRI study, we compared the brain activity of CB and SI processing either the spatial or the pitch properties of sounds carrying information in both domains (i.e., the same sounds were used in both tasks), using an adaptive procedure specifically designed to adjust for performance level. In addition to showing a substantial recruitment of the occipital cortex for sound processing in CB, we also demonstrate that auditory-spatial processing mainly recruits the right cuneus and the right middle occipital gyrus, two regions of the dorsal occipital stream known to be involved in visuospatial/motion processing in SI. Moreover, functional connectivity analyses revealed that these reorganized occipital regions are part of an extensive brain network including regions known to underlie audiovisual spatial abilities (i.e., intraparietal sulcus, superior frontal gyrus). We conclude that some regions of the right dorsal occipital stream do not require visual experience to develop a specialization for the processing of spatial information and to be functionally integrated in a preexisting brain network dedicated to this ability. [less ▲]

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See detailFunctional specifications of Teledu : ELECTRA deliverable D07.02
Baldewyns, Laurence; Reggers, Thérèse ULg; Poumay, Marianne ULg et al

Report (1997)

Detailed reference viewed: 17 (5 ULg)
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See detailFunctional study of Arabidopsis thaliana ASF/SF2-like pre-mRNA SR splicing factors
Stankovic, Nancy ULg; Tillemans, Vinciane; Leponce, Isabelle et al

Poster (2012, July 04)

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See detailFunctional study of Arabidopsis thaliana ASF/SF2-like pre-mRNA SR splicing factors
Stankovic, Nancy ULg; Tillemans, Vinciane; Hanikenne, Marc et al

Poster (2010, January 26)

Detailed reference viewed: 14 (5 ULg)
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See detailFunctional study of human tonsillar follicular dendritic cells.
Simar, L. J.; Lilet, Chantal ULg; Heinen, Ernst ULg et al

in Acta Oto-Rhino-Laryngologica Belgica (1984), 38(3), 278-87

Follicular dendritic cells (FDC) isolated from human tonsils and adenoids appear as round clusters where the FDC surround lymphoid cells. Using fluorescein and colloidal gold labelled antibodies we ... [more ▼]

Follicular dendritic cells (FDC) isolated from human tonsils and adenoids appear as round clusters where the FDC surround lymphoid cells. Using fluorescein and colloidal gold labelled antibodies we determined their surface antigens, the immunoglobulins they fix and the cells they envelope. Isolated FDC react with specific anti-FDC antibodies but also with an anti-monocyte/macrophage antibody and with anti-HLA-DR antibodies. They retain IgG, IgM, IgA and IgE but not IgD immunoglobulins; this retention occurs, according to our results, via their Fc and C3b receptors. The cells they envelope are mainly B cells, but also occasionally T helper cells. T suppressor cells were only rarely found in contact with FDC. We suggest that FDC create a micro-environment favourable to the proliferation and differentiation of B cells during the humoral response. [less ▲]

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See detailFunctional study of the pectoral spine stridulation mechanism in different mochokid catfishes
Parmentier, Eric ULg; Fabri, Grégory ULg; Kaatz, Ingrid et al

in Journal of Experimental Biology (2010), 213

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See detailFunctional study of the Ser/Arg-rich splicing factor SRSF5a during zebrafish embryonic development.
Joris, Marine ULg; Larbuisson, Arnaud ULg; Muller, Marc ULg et al

Poster (2013, April 18)

To investigate the role of the splicing factor SRSF5a during zebrafish embryonic development, we performed SRSF5a knockdown by morpholino microinjection and we analysed control and morphant transcriptomes ... [more ▼]

To investigate the role of the splicing factor SRSF5a during zebrafish embryonic development, we performed SRSF5a knockdown by morpholino microinjection and we analysed control and morphant transcriptomes using RNA sequencing. [less ▲]

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See detailFunctional suppression of the yeast msb3- mutation
Biver, Sophie ULg; Vandenbol, Micheline ULg

Poster (2010, September)

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See detailFunctional Templates and Their Applications to 3-D Object Recognition
Delanoy, Richard L.; Verly, Jacques ULg; Dudgeon, Dan E.

Conference (1992, March)

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See detailA functional test procedure for the improved commissioning of a VAV system
Andre, Philippe ULg; Lacôte, P.; Aparecida Silva, Cl. et al

Conference (2004, October)

In the context of the IEA40 research project, a specification of a “Functional Test Procedure” was progressively developed using the CA-MET building in Namur as real size test bench for the application ... [more ▼]

In the context of the IEA40 research project, a specification of a “Functional Test Procedure” was progressively developed using the CA-MET building in Namur as real size test bench for the application and validation of the procedure. The target system is a typical VAV system devoted to the ventilation, cooling and air conditioning functions. The procedure consists in a series of operations to be applied to the system, considered as a set of connected components and taking into account the following constraints: - time availability - intrinsic limitations of the system - reference performances The proposed procedure is divided in two sets of actions: - a number of preliminary actions allowing to take the best benefit of the subsequent phases: o identification of available measurement points o identification of actuators characteristics o identification of control strategies o aeraulic network calculations o selection of measurement techniques and experimental design - the verification tasks themselves: The philosophy of the verification process is to bring the system into a number of states whoch allowed each specific checks to be carried out. The organisation of the procedure tries to optimize the time spent for commissioning by logically sequencing the operations according to the following order: - test in manual operation - test in manual stop - test in normal operation - test at maximum flowrate - test at minimum flowrate - test in automatic stop The paper will provide the specific operations to be carried out at each step and will illustrate the whole process with the prototyping work performed on the CA-MET building in Namur. [less ▲]

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See detailFunctional traits are weak predictors of tree sapling growth worldwide
Paine, C. E. Timothy; Auge, Harald; Baraloto, Christopher et al

Conference (2013, June 26)

Functional traits, well-defined characteristics of organisms that influence or coordinate with ecological performance, promise that one may predict vital rates, such as survival and growth, by measuring a ... [more ▼]

Functional traits, well-defined characteristics of organisms that influence or coordinate with ecological performance, promise that one may predict vital rates, such as survival and growth, by measuring a few aspects of anatomy and physiology. Functional traits have been shown to correlate significantly, though weakly, with the relative growth rate (RGR) of trees in five Neotropical forests. Here, we estimated size-standardized height RGR for 26344 saplings of 240 species from 23 sites around the world using nonlinear models of growth. We then predicted the contribution of three functional traits (specific leaf area, wood density and seed mass) to individual RGR with a hierarchical Bayesian model. The three functional traits, alone and in combination, explained a relatively small amount of variance in RGR, with variation among continents and between temperate and tropical regions. We echo the calls for researchers to shift toward the measurement of 'hard' functional traits, with clear linkages to realized vital rates, rather than 'soft' functional traits, which are easier to measure but may only indicate the theoretical maxima of vital rates. Doing so would advance ecology onto a more solidly functional foundation. [less ▲]

Detailed reference viewed: 22 (7 ULg)