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See detailExpression of the interferon-alpha/beta-inducible bovine Mx1 dynamin interferes with replication of rabies virus
Leroy, Michael; Pire, Grégory; Baise, Etienne ULg et al

in Neurobiology of Disease (2006), 21(3), 515-521

Rabies is a fatal anthropozoonotic viral infection of the central nervous system that remains a serious public health problem in many countries. As several animal cases of spontaneous survival to ... [more ▼]

Rabies is a fatal anthropozoonotic viral infection of the central nervous system that remains a serious public health problem in many countries. As several animal cases of spontaneous survival to infection were reported and because type 1 interferons were shown to protect against the virus, it was suggested that innate resistance mechanisms exist. Among the antiviral proteins that are synthesized in response to interferon-alpha/beta stimulation, Mx proteins from several species are long known to block the replication of vesicular stomatitis virus (VSV). As both VSV and rabies virus belongs to the Rhabdoviridae family, this study was started with the aim to establish whether the anti-VSV activity of a mammalian Mx protein could be extended to rabies virus. This question was addressed by inoculating the virus onto a bovine Mx1 or human MxA-expressing Vero cell clone. Plaque formation was unambiguously blocked, and viral yields were reduced 100- to 1000-fold by bovine Mx1 expression for both SAG2 and SADB19 viral strains. In opposition, only SAG2 strain could be inhibited by the expression of human MxA protein. The effect of both proteins expression was then evaluated at the viral protein expression level. Again, boMx1 was able to repress protein expression in both strain, whereas only SAG2 proteins were inhibited in human MxA-expressing cells. These results suggest that protection conferred by interferon-alpha/beta against rabies could be, at least partially, attributable to the Mx pathway. Alternatively, bovine Mx1 could be unique in its ability to repress rabies virus which, if confirmed in vivo, would open an avenue for the development of new antirabies therapeutic strategies. [less ▲]

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See detailExpression of the metal homeostasis gene FRD3 in two Arabidopsis species
Charlier, Jean-Benoît ULg; Polese, Catherine ULg; Krämer, Ute et al

Poster (2011, August)

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See detailExpression of the metal homeostasis gene FRD3 in two Arabidopsis species
Charlier, Jean-Benoit ULg; Polese, Catherine; Motte, Patrick ULg et al

Poster (2010, January 26)

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See detailExpression of the monomeric 67-kd laminin-binding protein in human lymphomas as defined by MLuC5 monoclonal antibody and paraffin section immunohistochemistry.
Carbone, A.; Gloghini, A.; Colombatti, A. et al

in Human Pathology (1995), 26(5), 541-6

Interactions between cancer cells and laminin, a major component of basement membranes, are mediated through a large variety of cell surface proteins designated as laminin receptors. Among the above ... [more ▼]

Interactions between cancer cells and laminin, a major component of basement membranes, are mediated through a large variety of cell surface proteins designated as laminin receptors. Among the above proteins, a 67-kd monomeric high affinity laminin receptor (67 LR) has long been suspected to be involved in tumor progression. In this study we wished to establish whether the 67 LR molecule is detectable on tumor cells of Hodgkin's disease (HD) and non-Hodgkin's lymphomas (NHLs), to define its pattern of expression, and to assess the potential utility of 67 LR in differentiating these pathological entities. Morphological and immunohistological studies were performed on 85 specimens of HD and a series of 334 NHL specimens, including anaplastic large cell (ALC) (CD30-positive) lymphomas (73 specimens). For immunohistochemical assessment of the 67 LR we used the monoclonal antibody (MoAb) MLuC5 directed against the 67-kd laminin receptor on paraffin-embedded sections. Reed-Sternberg cells reacted with MLuC5 MoAb in four of 85 (4.7%) HD specimens. Among the NHL specimens, a MLuC5-positive reaction was expressed in 3.3% of B-cell lymphomas. They all belonged to the high grade subtypes. On the other hand, a MLuC5-positive reaction was detected in none of the T-cell lymphomas tested. In contrast to the results obtained with the other NHLs, in 30.2% of ALC (CD30-positive) lymphoma specimens, tumor cells reacted with MLuC5 MoAb. MLuC5-expressing ALC (CD30-positive) lymphoma cells were of either T-cell (six of 17 specimens), B-cell (three of 25 specimens), or undetermined phenotype (10 of 31 specimens). Our investigation has shown that 67 LR as shown by MLuC5 MoAb is detectable only in neoplastic cells of a fraction of ALC (CD30-positive) lymphomas and small subsets of B-cell high grade NHLs and HD. The restricted expression of the 67 LR molecule to ALC (CD30-positive) lymphomas provides a potential tool for the phenotypic separation of this pathological entity from HD and other lymphomas. Whether the detection of the 67 LR expression in these lymphoma subsets may be related to the aggressiveness of the disease remains to be ascertained. [less ▲]

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See detailExpression of the Mr 67,000 laminin receptor is an adverse prognostic indicator in human thyroid cancer: an immunohistochemical study.
Basolo, F.; Pollina, L.; Pacini, F. et al

in Clinical Cancer Research : An Official Journal of the American Association for Cancer Research (1996), 2(10), 1777-80

Increased expression of the Mr 67,000 laminin receptor (LR) is a consistent event which appears as cancer cells acquire an invasive and metastatic phenotype. The Mr 67,000 LR is one of the many laminin ... [more ▼]

Increased expression of the Mr 67,000 laminin receptor (LR) is a consistent event which appears as cancer cells acquire an invasive and metastatic phenotype. The Mr 67,000 LR is one of the many laminin-binding proteins able to interact with the major glycoprotein of basement membranes, laminin. The recent development of a specific monoclonal antibody directed against the Mr 67,000 LR MLuC5 has allowed us to study large retrospective groups of human cancers with the aim of correlating the Mr 67,000 LR expression to the clinical, pathological, and survival data of the patients. A significant correlation has already been established between the increased expression of Mr 67,000 LR and survival of patients with breast, colon, ovary, lung, and endometrial cancers. In this study, we investigated the possibility that the detection of Mr 67,000 LR in thyroid human cancers could also be of prognostic value. We analyzed the expression of Mr 67,000 LR with immunohistochemistry using MLuC5 antibodies in paraffin sections of 40 benign and 170 malignant thyroid human tumors. We found that Mr 67,000 LR was not usually detectable in normal thyroid tissues adjacent to the lesion. Only 3 of the 40 thyroid adenomas examined (7.5%) presented cells positive for Mr 67,000 LR. For the malignant thyroid tumors examined, we found that 22.3% of papillary thyroid carcinomas, 38% of follicular thyroid carcinomas, 40% of poorly differentiated carcinomas, 25% of medullary carcinomas, and 58.3% of anaplastic carcinomas expressed a high level of Mr 67,000 LR. Although no correlation between the Mr 67,000 LR expression and survival was found in patients with follicular thyroid carcinomas, papillary thyroid carcinomas, anaplastic carcinomas, and medullary carcinomas, there was a significant correlation in primary thyroid cancers. Our data represent the first extensive study of the Mr 67,000 LR expression in human thyroid cancers and strongly suggest that its detection could be of prognostic value in the investigation of primary thyroid cancers. [less ▲]

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See detailThe Expression of the Pituitary Growth Hormone Receptors by Blood Mononuclear Cells Can Be Modulated
Coumans, Bernard ULg; Thellin, Olivier ULg; Zorzi, Willy ULg et al

Poster (1997)

The action of the human pituitary growth hormone has been recently revealed in addition to its traditionnal effects on the staturo-ponderal growth and on the lipid and carbohydrate metabolism. We ... [more ▼]

The action of the human pituitary growth hormone has been recently revealed in addition to its traditionnal effects on the staturo-ponderal growth and on the lipid and carbohydrate metabolism. We demonstrated the presence of hGH-N receptors by cytometry in various peripheral mononuclear blood cells. These hGH-N-R were detected on B lymphocytes and on monocytes, contrarily to the T cells which were mostly negative. Since most blood cells are in a quiescent state, we have tested hGH-N-R expression by stimulated cultured blood cells. After PHA-L activation, most of T cells did express hGH-N-R. After SAC stimulation, B cells continue to express hGH-N-R. Monocytes can be induced to enhance the hGH-N-R expression. Since lymphocyte activation requires antigen recognition and costimulatory signals given by accessory or lymphoid cells, we can now hypothesise that activated cells bear higher numbers of certain receptors rendering them particularly receptive to messages, such as growth hormone, from the neuro-endocrine system. Quiescent B cells appear more sensitive to growth hormone signalling than T cells, but activation open the latter to the influence of growth hormone. The sensitivity of the immune system to signals of the neuro-endocrine system thus depends on its stimulation level. These observations reinforce the view that hGH-N is part of the immune system regulation machinery. [less ▲]

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See detailExpression of the pX products of the bovine leukemia virus
Willems, Luc ULg; Chen, G.; Kettmann, Richard ULg et al

in Cancer Letters (1987)

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See detailExpression of the somatolactin beta gene during zebrafish embryonic development
Lopez, Mauricio; Nica, G.; Motte, Patrick ULg et al

in Gene Expression Patterns (2006), 6(2), 156-161

Somatolactin (Sl) is a pituitary hormone closely related to prolactin (Prl) and growth hormone that was until now only found in various fish species. We isolated the cDNA coding for zebrafish Sl beta and ... [more ▼]

Somatolactin (Sl) is a pituitary hormone closely related to prolactin (Prl) and growth hormone that was until now only found in various fish species. We isolated the cDNA coding for zebrafish Sl beta and we identified the gene encoding this hormone. We also obtained a 1 kb genomic fragment corresponding to the sl beta upstream promoter region. Furthermore, the sl beta expression pattern was examined during zebrafish embryogenesis using whole-mount in situ hybridization. Sl beta mRNA is first detected in a single cell at the anterior border of the neural plate starting at 23 h post fertilization (hpf). Sl beta-expressing cells also express the transcription factor pit1 and are located close to prl-expressing cells. Using combined fluorescent in situ hybridization, we show that sl beta- and prl-expressing cells are clearly distinct at 29 hpf. Starting at 30 hpf, the number of sl beta positive cells increases and their location becomes more clearly distinct from lactotrope cells, in a more posterior position. At later stages (48 hpf), sl beta expression was observed posterior to growth hormone expression, again in a distinct cell type. We show that zebrafish mutants aal, as well as mutants in the pit1 gene, are deficient in sl beta expression. In conclusion, sl beta expression defines a new, additional cell type in zebrafish pituitary that depends on pit1 and aal for its differentiation. (C) 2005 Elsevier B.V. All rights reserved. [less ▲]

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See detailExpression of the zinc finger Egr1 gene during zebrafish embryonic development
Close, Renaud; Toro, Sabrina; Martial, Joseph ULg et al

in Mechanisms of Development (2002), 118(1-2), 269-72

Egr1 is a highly conserved zinc finger protein which plays important roles in many aspects of vertebrate development and in the adult. The cDNA coding for zebrafish Egr1 was obtained and its expression ... [more ▼]

Egr1 is a highly conserved zinc finger protein which plays important roles in many aspects of vertebrate development and in the adult. The cDNA coding for zebrafish Egr1 was obtained and its expression pattern was examined during zebrafish embryogenesis using whole-mount in situ hybridization. Egr1 mRNA is first detected in adaxial cells in the presomitic mesoderm between 11 and 20 h post-fertilization (hpf), spanning the 4-24 somite stages. Later, Egr1 expression is observed only in specific brain areas, starting at 21 hpf and subsequently increasing in distinct domains of the central nervous system, e.g. in the telencephalon, diencephalon and hypothalamus. Between 24 and 48 hpf, Egr1 is expressed in specific domains of the hypothalamus, mesencephalon, tegmentum, pharynx, retina, otic vesicle and heart. [less ▲]

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See detailExpression of transforming growth factor-alpha, epidermal growth factor, and epidermal growth factor receptor in follicles of human ovarian tissue before and after cryopreservation.
Qu, Jian Ping; NISOLLE, Michelle ULg; Donnez, Jacques

in Fertility and Sterility (2000), 74(1), 113-121

Objective: To study the expression of transforming growth factor-alpha (TGF-α), epidermal growth factor (EGF), and EGF receptor in follicles of human ovarian tissue. Design: A retrospective, controlled ... [more ▼]

Objective: To study the expression of transforming growth factor-alpha (TGF-α), epidermal growth factor (EGF), and EGF receptor in follicles of human ovarian tissue. Design: A retrospective, controlled comparative study. Setting: In vitro fertilization laboratory of a university hospital. Patient(s): Fifteen women with regular menstrual cycles who underwent laparoscopy and the biopsy of ovarian tissue. Intervention(s): Paraffin sections were prepared from ovarian tissues, followed by immunohistochemical staining of TGF-α, EGF, and EGF receptor. Main Outcome Measure(s): Immunostaining for TGF-α, EGF, and EGF receptor in follicles of fresh and frozen ovarian tissues. Result(s): Immunoreactivities for TGF-α and EGF receptor were observed simultaneously in the oocytes of primordial, primary, preantral, and antral follicles. Strong staining for TGF-α and EGF receptor was present in thecal cells. The TGF-α and EGF receptor was also expressed in some granulosa cells of primary to antral follicles. The EGF only stained weakly in the oocytes of primordial and primary follicles and in thecal cells. There was no difference in staining patterns for TGF-α, EGF, and EGF receptor between fresh and frozen ovarian tissues. Conclusion(s): The TGF-α and EGF receptor was expressed in primordial to antral follicles, indicating a role of TGF-α in regulating follicular development through binding to the EGF receptor. Freeze-thawing did not substantially alter immunoreactivites for TGF-α, EGF, and EGF receptor in frozen ovarian tissue. [less ▲]

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See detailExpression of transforming growth factors and their receptors is differentially modulated by reactive oxygen species and nitric oxide in human articular chondocytes
Ayache, N; Boumediene, K; Mathy-Hartert, M et al

in Osteoarthritis and Cartilage (2001), 9SB

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See detailExpression of type 2 orexin receptor in human endometrium and its epigenetic silencing in endometrial cancer.
Dehan, Pierre ULg; Canon, C.; Trooskens, G. et al

in Journal of Clinical Endocrinology and Metabolism (2013), 98(4), 1549-57

CONTEXT: Orexins A and B are neuropeptides that bind and activate 2 types of receptors. In addition to direct action in the brain, the orexinergic system has broader implications in peripheral organs, and ... [more ▼]

CONTEXT: Orexins A and B are neuropeptides that bind and activate 2 types of receptors. In addition to direct action in the brain, the orexinergic system has broader implications in peripheral organs, and it has been proposed to have a role in the induction of apoptosis. There are very few data on the endometrium. OBJECTIVE: The expression and epigenetic regulation of type 2 orexin receptor (OX2R) was investigated in the human endometrium as well as in endometrial endometrioid carcinoma (EEC). METHODS: OX2R localization was studied by immunohistochemistry in normal endometrium (n = 24) and in EEC (n = 32). The DNA methylation status of a CpG island located in the first exon of OX2R was analyzed by bisulfite sequencing in normal (n = 18), EEC (n = 34), and 3 endometrial cell lines. On the latter, mRNA expression and Western blotting as well as in vitro induction with orexin were performed. RESULTS: Expression of the OX2R protein was detected in normal endometrial epithelia, whereas it was frequently lacking in EEC. This loss was associated with hypermethylation of OX2R in EEC in comparison with normal endometrium (median CpG methylation percentages of 48.85% and 5.85%, respectively). In cell lines, hypermethylation correlated with weak OX2R expression. Additionally, in vitro treatment of the 3 EEC cell lines with orexins A and B did not result in proliferation change CONCLUSIONS: Altogether our data provide evidence for the epigenetic silencing of OX2R in EEC. The implication of the OX2R loss in tumoral progression remains to be elucidated. [less ▲]

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See detailExpression of zebrafish pax6b in pancreas is regulated by two enhancers containing highly conserved cis-elements bound by PDX1, PBX and PREP factors
Delporte, F. M.; Pasque, Vincent; Devos, Nathalie et al

in BMC Developmental Biology (2008), 8

BACKGROUND: PAX6 is a transcription factor playing a crucial role in the development of the eye and in the differentiation of the pancreatic endocrine cells as well as of enteroendocrine cells. Studies on ... [more ▼]

BACKGROUND: PAX6 is a transcription factor playing a crucial role in the development of the eye and in the differentiation of the pancreatic endocrine cells as well as of enteroendocrine cells. Studies on the mouse Pax6 gene have shown that sequences upstream from the P0 promoter are required for expression in the lens and the pancreas; but there remain discrepancies regarding the precise location of the pancreatic regulatory elements. RESULTS: Due to genome duplication in the evolution of ray-finned fishes, zebrafish has two pax6 genes, pax6a and pax6b. While both zebrafish pax6 genes are expressed in the developing eye and nervous system, only pax6b is expressed in the endocrine cells of the pancreas. To investigate the cause of this differential expression, we used a combination of in silico, in vivo and in vitro approaches. We show that the pax6b P0 promoter targets expression to endocrine pancreatic cells and also to enteroendocrine cells, retinal neurons and the telencephalon of transgenic zebrafish. Deletion analyses indicate that strong pancreatic expression of the pax6b gene relies on the combined action of two conserved regulatory enhancers, called regions A and C. By means of gel shift assays, we detected binding of the homeoproteins PDX1, PBX and PREP to several cis-elements of these regions. In constrast, regions A and C of the zebrafish pax6a gene are not active in the pancreas, this difference being attributable to sequence divergences within two cis-elements binding the pancreatic homeoprotein PDX1. CONCLUSION: Our data indicate a conserved role of enhancers A and C in the pancreatic expression of pax6b and emphasize the importance of the homeoproteins PBX and PREP cooperating with PDX1, in activating pax6b expression in endocrine pancreatic cells. This study also provides a striking example of how adaptative evolution of gene regulatory sequences upon gene duplication progressively leads to subfunctionalization of the paralogous gene pair. [less ▲]

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See detailExpression pattern of metalloproteinases and tissue inhibitors of matrix-metalloproteinases in cycling human endometrium
Goffin, Frédéric ULg; Munaut, Carine ULg; Frankenne, Francis et al

in Biology of Reproduction (2003), 69(3), 976-984

The cyclic growth, differentiation, and cell death of endometrium represents the most dynamic example of steroid-driven tissue turnover in human adults. Key effectors in these processes-matrix ... [more ▼]

The cyclic growth, differentiation, and cell death of endometrium represents the most dynamic example of steroid-driven tissue turnover in human adults. Key effectors in these processes-matrix metalloproteinases (MMPs) and their specific inhibitors (TIMPs)-are regulated by ovarian steroids and, locally, by cytokines. We used reverse transcription-polymerase chain reaction to evaluate the expression of both transcriptionally regulated molecules such as estrogen receptor-alpha, progesterone receptor, and prolactin and a large array of MMPs and TIMPs (MMP-1, -2, -3, -7, -8, -9, -11, -12, -19, -26, MT1-MMP, MT2-MMP, MT3-MMP, TIMP-1, -2, -3). Altogether, three distinct patterns of MMP and two patterns of TIMP expression were detected in cycling endometrium: 1). MMPs restricted to the menstrual period (MMPs-1, -3, -8, -9, -12); 2). MMPs and TIMPs expressed throughout the cycle (MMP-2, MT1-MMP, MT2-MMP, MMP-19, TIMP-1, and TIMP-2); 3). MMPs predominantly expressed during the proliferative phase (MMP-7, MMP-11, MMP-26, and MT3-MMP); and 4). TIMP-3, which, contrary to the other TIMPs, shows significant modulations, with maximum expression during the late secretory and menstrual phases. These specific patterns of MMP expression associated with each phase of the cycle may point to specific roles in the processes of menstruation, housekeeping activities, angiogenesis, tissue growth, and extracellular matrix remodeling. [less ▲]

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See detailExpression pattern of synaptic vesicle protein 2 (SV2) isoforms in patients with temporal lobe epilepsy and hippocampal sclerosis
CREVECOEUR, Julie ULg; Kaminski, RM; Rogister, Bernard ULg et al

in Neuropathology & Applied Neurobiology (2014), 40(2), 191-204

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See detailExpression patterns of miR-96, miR-182 and miR-183 in the development inner ear
Sacheli, Rosalie ULg; Nguyen, Laurent ULg; Borgs, Laurence ULg et al

in Gene Expression Patterns (2009)

MicroRNAs (miRNAs) constitute a class of small non-coding endogenous RNAs that downregulate gene expression by binding to 3' untranslated region (UTR) of target messenger RNAs. Although they have been ... [more ▼]

MicroRNAs (miRNAs) constitute a class of small non-coding endogenous RNAs that downregulate gene expression by binding to 3' untranslated region (UTR) of target messenger RNAs. Although they have been found to regulate developmental and physiological processes in several organs and tissues, their role in the regulation of the inner ear transcriptome remains unknown. In this report, we have performed systematic in situ hybridization to analyze the temporal and spatial distribution of three miRNAs (miR-96, mR-182, and mR-183) that are likely to arise from a single precursor RNA during the development and the maturation of the cochlea. Strikingly we found that the expression of mR-96, mR-182 and mR-183 was highly dynamic during the development of the cochlea, from the patterning to the differentiation of the main cochlear structures. [less ▲]

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See detailExpression politique et activisme en ligne en contexte autoritaire. Une analyse du cas tunisien
Lecomte, Romain ULg

in Réseaux : Communication - Technologie - Société (2013), 181

In the popular uprising in Tunisia that led to President Ben Ali’s departure on 14 January 2011, Internet was used extensively to broadcast information both nationally and internationally, and to put out ... [more ▼]

In the popular uprising in Tunisia that led to President Ben Ali’s departure on 14 January 2011, Internet was used extensively to broadcast information both nationally and internationally, and to put out calls for mobilization. Its role has however often been analyzed only superficially. In this article, rather than focusing on these critical circumstances, we look back at the history of Tunisians’ uses of Internet for protest purposes. This type of use emerged in the late 1990s and grew rapidly thereafter. We break this history down into three main periods corresponding to three configurations characterized by new actors, new technical systems, and new forms of political expression and collective protest action. [less ▲]

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See detailExpression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study.
Borlon, Celine; Weemaels, Geoffroy; Godard, Patrice et al

in Biogerontology (2008), 9(3), 197-208

It is often described that it is difficult to really discriminate the cause of intrinsic skin aging. The aim of this study was to compare the profiles of expression of senescence-associated genes in ... [more ▼]

It is often described that it is difficult to really discriminate the cause of intrinsic skin aging. The aim of this study was to compare the profiles of expression of senescence-associated genes in biopsies of dermis from young and old human donors. TGF-beta1 was up-regulated in the dermis of old donors as well as the TGF-beta1-regulated genes. The anti-oxidant enzymes Selenium-dependent Glutathione peroxidase and Glutatione S-Transferase Theta 1 were also up-regulated in old dermis as well as Tumor Necrosis Factor Receptor Superfamily 1A. None of these genes had altered expression level in skin fibroblasts embedded in a collagen matrix and exposed to sublethal doses of UVB, suggesting their involvement in intrinsic aging. This study represents a proof-of-concept of larger whole transcriptome studies where all avenues should be used to subtract changes in gene expression due to extrinsic aging from changes potentially due to intrinsic aging. [less ▲]

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