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See detailFully automated heart rate variability assessment in dairy cows
Janda, Jozef; Guyot, Hugues ULg; Sandersen, Charlotte ULg et al

in Acta Physiologica (2006, May), 187(Suppl 651), -16

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See detailA Fully Automated High-Performance Liquid Chromatographic Method for the Determination of Indomethacin in Plasma
Hubert, Philippe ULg; Renson, M.; Crommen, Jacques ULg

in Journal of Pharmaceutical & Biomedical Analysis (1989), 7(12), 1819-27

A fully automated method is described, which enables the determination of indomethacin in plasma by reversed-phase HPLC following on-line sample enrichment and clean-up on a short pre-column. The plasma ... [more ▼]

A fully automated method is described, which enables the determination of indomethacin in plasma by reversed-phase HPLC following on-line sample enrichment and clean-up on a short pre-column. The plasma sample is introduced directly into the column switching system. The pre-column, filled with a pellicular bonded phase, is first washed with phosphate buffer, pH 7.4. The compounds retained on the pre-column are then eluted in the fore-flush mode and separated on an octadecylsilica column with a methanolic phosphate buffer (pH 7.4) mobile phase. Indomethacin is determined spectrophotometrically at 254 or 260 nm. The effect of changes in the pH and flow rate of the washing eluent are studied. Under the conditions selected, memory effects can be avoided, the absolute recovery of the drug is 70% and the limit of detection 10 ng ml-1 for a 100 microliter injection of plasma. At a concentration of 100 ng ml-1, the relative standard deviations (RSD) are 1.7% (within-day) and 3.5% (between-day), respectively. [less ▲]

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See detailFully automated LC method for the determination of sotalol in human plasma using restricted access material with cation exchange properties for sample clean-up
Rbeida, O.; Christiaens, B.; Chiap, Patrice ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (2003), 32(4-5), 829-838

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See detailFully automated method for the liquid chromatographic determination of cyproterone acetate in plasma using restricted access material for sample pre-treatment
Christiaens, B.; Chiap, Patrice ULg; Rbeida, O. et al

in Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences (2003), 795(1), 73-82

A new fully automated method for the quantitative analysis of an antiandrogenic substance, cyproterone acetate (CPA), in plasma samples has been developed using on-line solid-phase extraction (SPE) prior ... [more ▼]

A new fully automated method for the quantitative analysis of an antiandrogenic substance, cyproterone acetate (CPA), in plasma samples has been developed using on-line solid-phase extraction (SPE) prior to the determination by reversed-phase liquid chromatography (LC). The automated method was based on the use of a precolumn packed with an internal-surface reversed-phase packing material (LiChrospher RP-4 ADS) for sample clean-up coupled to LC analysis on an octadecyl stationary phase using a column-switching system. A 200-mul volume of plasma sample was injected directly on the precolumn packed with restricted access material using a mixture of water-acetonitrile (90:10, v/v) as washing liquid. The analyte was then eluted in the back-flush mode with the LC mobile phase which consisted of a mixture of phosphate buffer, pH 7.0-acetonitrile (54:46, v/v). The elution profiles of CPA and blank plasma samples on the precolumn and the time needed for analyte transfer from the precolumn to the analytical column were determined. Different compositions of washing liquid and mobile phase were tested to reduce the interference of plasma endogenous components. UV detection was achieved at 280 nm. Finally, the developed method was validated using a new approach, namely the application of the accuracy profile based on the interval confidence at 90% of the total measurement error (bias+standard deviation). The limit of quantification of cyproterone acetate in plasma was determined at 15 ng ml(-1). The validated method should be applicable to the determination of CPA in patients treated by at least 50 mg day(-1). (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

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See detailFully automated method for the liquid chromatographic-tandem mass spectrometric determination of cyproterone acetate in human plasma using restricted access material for on-line sample clean-up
Christiaens, B.; Fillet, Marianne ULg; Chiap, Patrice ULg et al

in Journal of Chromatography. A (2004), 1056(1-2), 105-110

A new automated method for the quantitative analysis of cyproterone acetate (CPA) in human plasma has been developed using on-line solid phase extraction (SPE) prior to the LC-MS/MS determination. The ... [more ▼]

A new automated method for the quantitative analysis of cyproterone acetate (CPA) in human plasma has been developed using on-line solid phase extraction (SPE) prior to the LC-MS/MS determination. The method was based on the use of a pre-column packed with internal-surface reversed-phase material (LiChrospher RP-4 ADS, 25 mm x 2 mm) for sample clean-up coupled to LC separation on an octadecyl silica stationary phase by means of a column switching system. A 30 microl plasma sample volume was injected directly onto the pre-column using a mixture of water, acetonitrile and formic acid (90:10:0.1 (v/v/v)) adjusted to pH 4.0 with diluted ammonia as washing liquid. The analyte was then eluted in the back-flush mode with the LC mobile phase consisting of water, methanol and formic acid (10:90:0.1 (v/v/v)). The dispensing flow rates of the washing liquid and the LC mobile phase were 300 microl min(-1). Medroxyprogesterone acetate (MPA) was used as internal standard. The MS ionization of the analytes was achieved using electrospray (ESI) in the positive ion mode. The pseudomolecular ionic species of CPA and MPA (417.4 and 387.5) were selected to generate daughter ions at 357.4 and 327.5, respectively. Finally, the developed method was validated according to a new approach using accuracy profiles as a decision tool. Very good results with respect to accuracy, detectability, repeatability, intermediate precision and selectivity were obtained. The LOQ of cyproterone acetate was 300 pg ml(-1). [less ▲]

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See detailFully automated method for the liquid chromatographic-tandem mass spectrometric determination of cyproterone acetate in human plasma using restricted access material for on-line sample clean-up.
Christians, Benoit; Fillet, Marianne ULg; Chiap, Patrice ULg et al

in Journal of Chromatography. A (2004), 1056(1-2), 105-10

A new automated method for the quantitative analysis of cyproterone acetate (CPA) in human plasma has been developed using on-line solid phase extraction (SPE) prior to the LC-MS/MS determination. The ... [more ▼]

A new automated method for the quantitative analysis of cyproterone acetate (CPA) in human plasma has been developed using on-line solid phase extraction (SPE) prior to the LC-MS/MS determination. The method was based on the use of a pre-column packed with internal-surface reversed-phase material (LiChrospher RP-4 ADS, 25 mm x 2 mm) for sample clean-up coupled to LC separation on an octadecyl silica stationary phase by means of a column switching system. A 30 microl plasma sample volume was injected directly onto the pre-column using a mixture of water, acetonitrile and formic acid (90:10:0.1 (v/v/v)) adjusted to pH 4.0 with diluted ammonia as washing liquid. The analyte was then eluted in the back-flush mode with the LC mobile phase consisting of water, methanol and formic acid (10:90:0.1 (v/v/v)). The dispensing flow rates of the washing liquid and the LC mobile phase were 300 microl min(-1). Medroxyprogesterone acetate (MPA) was used as internal standard. The MS ionization of the analytes was achieved using electrospray (ESI) in the positive ion mode. The pseudomolecular ionic species of CPA and MPA (417.4 and 387.5) were selected to generate daughter ions at 357.4 and 327.5, respectively. Finally, the developed method was validated according to a new approach using accuracy profiles as a decision tool. Very good results with respect to accuracy, detectability, repeatability, intermediate precision and selectivity were obtained. The LOQ of cyproterone acetate was 300 pg ml(-1). [less ▲]

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See detailFully Automated Preparation and Conjugation of N-Succinimidyl 4-[(18)F]Fluorobenzoate ([ (18)F]SFB) with RGD Peptide Using a GE FASTlab Synthesizer.
Thonon, David ULg; Goblet, D.; Goukens, Eve ULg et al

in Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging (2011)

PURPOSE: The aim of this work was to automate the radiosynthesis of [(18)F]SFB, a widely used reagent for the labeling of biomolecules with (18)F on a new generation commercial synthesis module (FASTLab ... [more ▼]

PURPOSE: The aim of this work was to automate the radiosynthesis of [(18)F]SFB, a widely used reagent for the labeling of biomolecules with (18)F on a new generation commercial synthesis module (FASTLab, GE Healthcare). PROCEDURES: Two synthesis approaches were implemented on this module: the classical "two-pot radiosynthesis" and the more recently described "one-pot" method. RESULTS: The "two-pot" approach affords [(18)F]SFB with a 42% decay-corrected yield in 57 min (n = 24) with a chemical purity sufficient to avoid an intermediate HPLC purification. The recently established "one-pot" method, afforded a product with a lower chemical purity, in the conditions used in this report. The lower d.c. yield obtained (32% (n = 15)) was related to the low (18)F labeling yields obtained in MeCN compared with DMSO. The subsequent conjugation step with a RGD (PRGD2) peptide was also successfully automated. CONCLUSIONS: The formulated [(18)F]FPRGD2 was obtained without any operator manipulation with a d.c. yield of 13% +/- 3% (n = 13) in 130 min, a radiochemical purity >98% and a specific activity of 140 +/- 40 TBq/mmol. [less ▲]

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See detailFully Automated Production of 68Ga-DOTA-NOC with a Trasis miniAIO® Synthesizer
Léonard, Marc ULg; Aerts, Joël ULg; Voccia, Samuel et al

Poster (2013, October 22)

Detailed reference viewed: 54 (14 ULg)
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See detailA fully coupled anisotropic elasto-plastic-damage model
Cescotto, Serge ULg; Wauters, Michaël; Habraken, Anne ULg et al

in Handbook of Materials Behavior Models (2001)

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See detailA fully coupled elasto-plastic damage model applied to anisotropic materials
Wauters, M.; Habraken, Anne ULg; Duchene, Laurent ULg

in Tassoulas, J. L. (Ed.) Proceedings of the 14th Engineering Mechanics Conference (2000)

In this paper, an elastoplastic energy-based anisotropic damage model for ductile fracture is described. A calibration method is also presented. The potential applicability of this model is illustrated by ... [more ▼]

In this paper, an elastoplastic energy-based anisotropic damage model for ductile fracture is described. A calibration method is also presented. The potential applicability of this model is illustrated by numerical examples of tensile test and Forming Limit Diagram establishment on a steel. [less ▲]

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See detailA fully coupled elasto-plastic damage model applied to anisotropic materials
Wauters, M.; Habraken, Anne ULg; Duchene, Laurent ULg

(2000)

In this paper, an elastoplastic energy-based anisotropic damage model for ductile fracture is described. A calibration method is presented. The potential applicability of this model is illustrated by a ... [more ▼]

In this paper, an elastoplastic energy-based anisotropic damage model for ductile fracture is described. A calibration method is presented. The potential applicability of this model is illustrated by a numerical example of deep drawing process realised on two steels. [less ▲]

Detailed reference viewed: 41 (10 ULg)
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See detailA fully coupled elastoplastic damage modeling and fracture criteria in metalforming processes
Zhu, Y. Y.; Cescotto, Serge ULg; Habraken, Anne ULg

in Journal of Materials Processing Technology (1992), 32

Detailed reference viewed: 30 (3 ULg)