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See detailEverolimus Plus Exemestane Versus Everolimus or Capecitabine Monotherapy in Breast Cancer : BOLERO-6
Ejlertsen, Bent; JERUSALEM, Guy ULg; Hurvitz, Sara et al

Poster (2013, March)

The data from this trial will provide insight into the safety and efficacy of the combination of EVE and EXE versus EVE or capecitabine monotherapy in women with ER+, HER2- ABC progressing on/after prior ... [more ▼]

The data from this trial will provide insight into the safety and efficacy of the combination of EVE and EXE versus EVE or capecitabine monotherapy in women with ER+, HER2- ABC progressing on/after prior LET or ANA. [less ▲]

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See detailEverybody knows what IRT is... (really?)
Magis, David ULg

Conference (2012, March 27)

Item response theory (IRT) is not only a famous acronym, it also covers a broad range of statistical and psychometric models, methods, and applications in psychology and educational science. However, IRT ... [more ▼]

Item response theory (IRT) is not only a famous acronym, it also covers a broad range of statistical and psychometric models, methods, and applications in psychology and educational science. However, IRT looks somewhat misunderstood or even esoteric to the non-statisticians and non-psychometricians. The main goal of this talk is to briefly and clearly present the main aspects of traditional IRT: objectives, assumptions, basic models, and basic estimation methods. As a secondary purpose, extensions of IRT to more complex situations (polytomous data, multidimensional and hierarchical models, computerized adaptive testing, differential item functioning, nonparametric IRT) are briefly outlined. This talk will be as little technical as possible, focusing on the main concepts and applications of this theory. [less ▲]

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See detailHet everzwijn en het geheimzinnige eiland
Gerkens, Jean-François ULg

Scientific conference (2009, March 19)

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See detailEvi1 is specifically expressed in the distal tubule and duct of the Xenopus pronephros and plays a role in its formation.
Van Campenhout, Claude; Nichane, Massimo; Antoniou, Aline et al

in Developmental Biology (2006), 294(1), 203-19

The ecotropic viral integration site 1 (Evi1) and related MEL1 (MDS1/Evi1-like gene 1) genes are zinc finger oncogenic transcription factors involved in myeloid leukaemia. Here, we show that in Xenopus ... [more ▼]

The ecotropic viral integration site 1 (Evi1) and related MEL1 (MDS1/Evi1-like gene 1) genes are zinc finger oncogenic transcription factors involved in myeloid leukaemia. Here, we show that in Xenopus, Evi1 and MEL1 have partially overlapping restricted embryonic expression profiles. Within the pronephros, Evi1 and MEL1 are sequentially expressed within the distal tubule and duct compartments, Evi1 transcription being detected prior to any sign of pronephric morphogenesis. In the pronephros of zebrafish embryos, Evi1 expression is restricted to the posterior portion of the duct, the anterior portion having characteristics of proximal tubules. In the Xenopus pronephros, Evi1 expression is upregulated by retinoid signaling and repressed by overexpression of xWT1 and by Notch signaling. Overexpression of Evi1 from late neurula stage specifically inhibits the expression of proximal tubule and glomus pronephric markers. We show that the first zinc finger and CtBP interaction domains are required for this activity. Overexpression of a hormone-inducible Evi1-VP16 antimorphic fusion with activation at neurula stage disrupts distal tubule and duct formation and expands the expression of glomus markers. Although overexpression of this construct also causes in many embryos a reduction of proximal tubule markers, embryos with expanded and ectopic staining have been also observed. Together, these data indicate that Evi1 plays a role in the proximo-distal patterning of the pronephros and suggest that it may do so by functioning as a CtBP dependent repressor. [less ▲]

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See detailEVI1-mediated down regulation of MIR449A is essential for the survival of EVI1 positive leukaemic cells.
De Weer, An; Van der Meulen, Joni; Rondou, Pieter et al

in British Journal of Haematology (2011), 154(3), 337-48

Chromosomal rearrangements involving the MECOM (MDS1 and EVI1 complex) locus are recurrent genetic events in myeloid leukaemia and are associated with poor prognosis. In this study, we assessed the role ... [more ▼]

Chromosomal rearrangements involving the MECOM (MDS1 and EVI1 complex) locus are recurrent genetic events in myeloid leukaemia and are associated with poor prognosis. In this study, we assessed the role of MECOM locus protein EVI1 in the transcriptional regulation of microRNAs (miRNAs) involved in the leukaemic phenotype. For this, we profiled expression of 366 miRNAs in 38 MECOM-rearranged patient samples, normal bone marrow controls and MECOM (EVI1) knock down/re-expression models. Cross-comparison of these miRNA expression profiling data showed that MECOM rearranged leukaemias are characterized by down regulation of MIR449A. Reconstitution of MIR449A expression in MECOM-rearranged cell line models induced apoptosis resulting in a strong decrease in cell viability. These effects might be mediated in part by MIR449A regulation of NOTCH1 and BCL2, which are shown here to be bona fide MIR449A targets. Finally, we confirmed that MIR449A repression is mediated through direct promoter occupation of the EVI1 transcriptional repressor. In conclusion, this study reveals MIR449A as a crucial direct target of the MECOM locus protein EVI1 involved in the pathogenesis of MECOM-rearranged leukaemias and unravels NOTCH1 and BCL2 as important novel targets of MIR449A. This EVI1-MIR449A-NOTCH1/BCL2 regulatory axis might open new possibilities for the development of therapeutic strategies in this poor prognostic leukaemia subgroup. [less ▲]

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See detailEvidence and analysis of 2012 Greenland records from spaceborne observations, a regional climate model and reanalysis data
Tedesco, M.; Fettweis, Xavier ULg; Mote, T. et al

in Cryosphere (The) (2013), 7

A combined analysis of remote sensing observations, regional climate model (RCM) outputs and reanalysis data over the Greenland ice sheet provides evidence that multiple records were set during summer ... [more ▼]

A combined analysis of remote sensing observations, regional climate model (RCM) outputs and reanalysis data over the Greenland ice sheet provides evidence that multiple records were set during summer 2012. Melt extent was the largest in the satellite era (extending up to ∼97% of the ice sheet) and melting lasted up to ∼2 months longer than the 1979–2011 mean. Model results indicate that near surface temperature was ∼3 standard deviations (σ) above the 1958–2011 mean, while surface mass balance (SMB) was ∼3σ below the mean and runoff was 3.9σ above the mean over the same period. Albedo, exposure of bare ice and surface mass balance also set new records, as did the total mass balance with summer and annual mass changes of, respectively, −627 Gt and −574 Gt, 2σ below the 2003–2012 mean. We identify persistent anticyclonic conditions over Greenland associated with anomalies in the North Atlantic Oscillation (NAO), changes in surface conditions (e.g., albedo, surface temperature) and preconditioning of surface properties from recent extreme melting as major driving mechanisms for the 2012 records. Less positive if not increasingly negative SMB will likely occur should these characteristics persist. [less ▲]

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See detailEvidence and purification of a novel bacterial dehydrogenase : a vanillin : NAD (P) oxydoreductase.
Bare, G.; Moukil, M.; Swiatkowski, Th. et al

Poster (1996, August)

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See detailEvidence based medicine - Tendon and platelet-rich plasma (PRP)
Kaux, Jean-François ULg

in Annual Congress RSBPRM (2014, December 06)

Platelets have known roles in coagulation, inflammatory processes, and immunity modulation; they also have ‘‘restorative’’ properties. Indeed, during degranulation, platelets release different cytokines ... [more ▼]

Platelets have known roles in coagulation, inflammatory processes, and immunity modulation; they also have ‘‘restorative’’ properties. Indeed, during degranulation, platelets release different cytokines and growth factors (VEGF, PDGF, TGF-B, IGF-I, and HGF) that promote angiogenesis, tissue remodeling (bone, skin, muscle, tendon, etc.), and wound healing. PRP is obtained by centrifuging autologous blood to obtain a concentration of platelets, usually between 3 and 10 times that of whole blood, depending on the isolation method. For this reason, different PRP preparation techniques cannot provide a consistently identical final product, but there is currently no international consensus on this issue. Overall, PRP could be an attractive therapeutic option for treating chronic musculoskeletal conditions, such as tendinopathy or plantar fasciitis. Tendons do not have a high metabolic index. Growth factors released by platelets promote tenocyte proliferation, stimulate angiogenesis and have analgesic properties. Thus it could stimulate and accelerate tissue regeneration in animal models. In addition to PRP, optimal tissue quality requires the application of mechanical loads. PRP should be considered for chronic tendinopathies. Indeed, the goal is to initiate an acute inflammatory reaction that quickly moves on to the proliferative phase that involves collagen synthesis. The latter is necessary for appropriate tendon healing. PRP should therefore not be used for acute tendinitis or tenosynovitis. Even if most of the preclinical studies showed that PRP stimulates the tendon healing process, clinical series remain more controversial. Based on literature and our clinical experience, we suggest some ideas for improving this treatment. Optimization of the technique for collecting the PRP is paramount. Different risk factors must be corrected before infiltration, and chronic tendinopathies must be carefully selected. Finally, post-infiltration rehabilitation remains absolutely necessary. Standardisation of the use of PRP remains necessary in order to optimise the results. [less ▲]

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See detailEvidence based policies?
Blad, John; Lauwaert, Katrien ULg

in Vanfraechem, Inge; Aertsen, Ivo (Eds.) Restorative Justice Realities. Empirical Research in a European Context (2010)

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See detailEvidence Based Practice
Bruyère, Olivier ULg

Learning material (2011)

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See detailEvidence for a differentiated chromosomal race north of classical south European refuge areas in the garden dormouse Eliomys quercinus
Libois, Roland ULg; Ramalhinho, Maria da Graça; Rosoux, René

in Acta Theriologica (2012), 57(4), 313-320

The dormouse Eliomys quercinus is a forest rodent undergoing long periods of winter hibernation. The species presents a surprisingly large diversity of chromosomal races, which geographic distribution was ... [more ▼]

The dormouse Eliomys quercinus is a forest rodent undergoing long periods of winter hibernation. The species presents a surprisingly large diversity of chromosomal races, which geographic distribution was shown recently to predate the Pleistocene glaciations. Previously reported data on the karyotypes of the garden dormouse in France come from the northeast of the country, where the 2N050 race occurs. New data are presented from specimens trapped near the Atlantic coast (departments of Vendée and Charente-Maritime), in the Pyrenees, the Alps and in the Massif Central. The French Alpine chain, close to the Italian border, is inhabited by the 2N054 race. A karyotype with 2N048 chromosomes, of Iberian type, is found north of the Pyrenees, near the central Atlantic coast and also in the south of the Massif Central, whereas the 2N050 race occurs in the north of the massif. A hybrid between these two races (2N049) was found in Vendée. These facts reveal that neither the Pyrenees nor the Alps constitute a biogeographic barrier to the dormouse and strongly suggest that the present population of northern France derives from a postglacial recolonisation movement initiated in the southernmost regions of France or in the Rhône valley. [less ▲]

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See detailEvidence for a mitochondrial synthesis of thiamine triphosphate in the rat brain
Gangolf, M; Wins, P; Thiry, Marc ULg et al

Conference (2008)

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See detailEvidence for a Modulatory Role of Ih on the Firing of a Subgroup of Midbrain Dopamine Neurons
Seutin, Vincent ULg; Massotte, Laurent ULg; Renette, M. F. et al

in Neuroreport (2001), 12(2), 255-8

A previous investigation has suggested that the hyperpolarization-activated cation current Ih does not contribute to the spontaneous firing of midbrain dopaminergic neurons. This conclusion was reached ... [more ▼]

A previous investigation has suggested that the hyperpolarization-activated cation current Ih does not contribute to the spontaneous firing of midbrain dopaminergic neurons. This conclusion was reached using Cs(-1). We have re-examined this question with extracellular recordings in slices using the more specific blocker ZD7288. In two-thirds of the cells, low concentrations of ZD7288 induced a decrease of the spontaneous firing. The maximal inhibition was about 40% and the mean IC50 was 1.6 microM. This effect was probably direct because it persisted in the presence of antagonists of various receptors. These concentrations of ZD7288 had no effect in the remaining one third of the examined cells. However, the highest concentration of ZD7288 (300 microM) abolished the firing of all dopaminergic neurons, probably by a mechanism unrelated to the blockade of Ih. We conclude that Ih controls to a certain extent the firing of a majority of midbrain dopaminergic neurons. [less ▲]

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See detailEvidence for a multiprotein gamma-2 herpesvirus entry complex.
Gillet, Laurent ULg; Stevenson, Philip G

in Journal of Virology (2007), 81(23), 13082-91

Herpesviruses use multiple virion glycoproteins to enter cells. How these work together is not well understood: some may act separately or they may form a single complex. Murine gammaherpesvirus 68 (MHV ... [more ▼]

Herpesviruses use multiple virion glycoproteins to enter cells. How these work together is not well understood: some may act separately or they may form a single complex. Murine gammaherpesvirus 68 (MHV-68) gB, gH, gL, and gp150 all participate in entry. gB and gL are involved in binding, gB and gH are conserved fusion proteins, and gp150 inhibits cell binding until glycosaminoglycans are engaged. Here we show that a gH-specific antibody coprecipitates gB and thus that gH and gB are associated in the virion membrane. A gH/gL-specific antibody also coprecipitated gB, implying a tripartite complex of gL/gH/gB, although the gH/gB association did not require gL. The association was also independent of gp150, and gp150 was not demonstrably bound to gB or gH. However, gp150 incorporation into virions was partly gL dependent, suggesting that it too contributes to a single entry complex. gp150- and gL- gp150- mutants bound better than the wild type to B cells and readily colonized B cells in vivo. Thus, gp150 and gL appear to be epithelial cell-adapted accessories of a core gB/gH entry complex. The cell binding revealed by gp150 disruption did not require gL and therefore seemed most likely to involve gB. [less ▲]

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See detailEvidence for a Non-Gabaergic Action of Quaternary Salts of Bicuculline on Dopaminergic Neurones
Seutin, Vincent ULg; Scuvée-Moreau, Jacqueline ULg; Dresse, Albert ULg

in Neuropharmacology (1997), 36(11-12, Nov-Dec), 1653-7

Intracellular recordings were made from neurones, presumed to be dopaminergic, in the rat midbrain slice preparation. Bicuculline methiodide (BMI) and methochloride (BMC) reversibly blocked the slow ... [more ▼]

Intracellular recordings were made from neurones, presumed to be dopaminergic, in the rat midbrain slice preparation. Bicuculline methiodide (BMI) and methochloride (BMC) reversibly blocked the slow, apamin-sensitive component of the afterhyperpolarization in these cells. The IC50 for this effect was about 26 microM. In comparison, BMC antagonized the input resistance decrease evoked by muscimol (3 microM) with an IC50 of 7 microM. The base of bicuculline was less potent in blocking the slow afterhyperpolarization. SR95531 (2-[carboxy-3'-propyl]-3-amino-6-paramethoxy-phenyl-pyridaziniu m bromide), another competitive GABA(A) antagonist, and picrotoxin, a non-competitive GABA(A) antagonist, also blocked the action of muscimol (IC50s: 2 and 12 microM respectively), but had no effect on the afterhyperpolarization at a concentration of up to 100 microM. Moreover, 100 microM SR95531 did not affect the blockade of the afterhyperpolarization by BMC. This blockade persisted in the presence of tetrodotoxin and was apparently not due to a reduction of calcium entry, suggesting that it involved a direct action on the channels which mediate this afterhyperpolarization. These results strongly suggest that quaternary salts of bicuculline act on more than one target in the central nervous system. Thus, the involvement of GABA(A) receptors in a given effect cannot be proven solely on the basis of its blockade by these agents. [less ▲]

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See detailEvidence for a partial epithelial-mesenchymal transition in postnatal stages of rat auditory organ morphogenesis
Johnen, Nicolas ULg; Cloes, Marie ULg; Thelen, Nicolas ULg et al

Poster (2012, May 04)

An epithelial-mesenchymal transition is a biological process that allows a polarized epithelial cell to undergo multiple biochemical changes that enable it to assume a mesenchymal cell phenotype. During ... [more ▼]

An epithelial-mesenchymal transition is a biological process that allows a polarized epithelial cell to undergo multiple biochemical changes that enable it to assume a mesenchymal cell phenotype. During this process, epithelial cells loosen cell-cell adhesion, module their polarity and rearrange their cytoskeleton: intermediate filaments typically switch from cytokeratin to vimentin. They also enhance their motility capacity. The epithelial-mesenchymal transition plays key roles in the formation of the body plan and in the differentiation of multiple tissues and organs but it is also involved in tissue repair, tissue homeostasis, fibrosis, and carcinoma progression. Until now, epithelial-mesenchymal transition has been rarely mentioned in the inner ear organogenesis. In chick, epithelial-mesenchymal transition has been reported as a possible mechanism of semicircular canal morphogenesis. More recently, an in vitro study has also indicated that sensory epithelial cells from mouse utricle can undergo an epithelial-mesenchymal transition to become cells expressing features of prosensory cells. By contrast, epithelial-mesenchymal transition has never been observed during auditory organ morphogenesis. The auditory organ, the organ of Corti, is a highly specialized structure composed by specific cellular types. The sensory cells are characterized by stereocilia at their apex and are necessary for the sound perception. Theses cells are supported by supporting cells. Based on their morphology and physiology, at least four types of supporting cells can be identified in the organ of Corti: inner and outer pillar cells, phalangeal cell and Deiter’s cells. The inner pillar cells and outer pillar cells combine to form the tunnel of Corti, a fluid filled triangular space that separates the single row of inner hair cells from the first row of outer hair cells. The Nuel spaces are another interval in the organ of Corti that is situated between the outer pillar cells and the different rows of outer hair cells and Deiters cells. To determine whether an epithelial-mesenchymal transition may play a role in the morphogenesis of the auditory organ, we studied the spatial localization of several epithelial-mesenchymal transition markers, the cell-cell adhesion molecules and intermediate filament cytoskeletal proteins, in epithelium of the dorsal cochlea during development of the rat organ of Corti from 18th embryonic day until 25th postnatal day. We examined by confocal microscopy immunolabelings on cryosections of whole cochleae with antibodies anti-cytokeratins as well as with antibodies anti-vimentin, anti-E-cadherin and anti-beta-catenin.Our results showed a partial loss of E-cadherin and beta-catenin between supporting cells at P8 and P12, respectively, and a temporary appearance of vimentin in pillar cells and Deiters between P8 and P10. Our results show a local loss of adhesion between supporting cells of the OC from P8, an increase expression of cytokeratins in supporting cells around P10 and a temporary appearance of vimentin in supporting cells at P8-10. These observations suggest that a partial epithelial-mesenchymal transition might be involved in the remodeling of the Corti organ during the postnatal stages of development in rat. [less ▲]

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