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See detailFractionation of the DD-carboxypeptidase-transpeptidase activities solubilized from membranes of Escherichia coli K12, strain 44
Pollock, Jerry J.; Nguyen-Disteche, Martine; Ghuysen, Jean-Marie ULg et al

in European Journal of Biochemistry (1974), 41(3), 439-446

A transpeptidase activity in Escherichia coli was measured independently of other enzymes involved in peptidoglycan synthesis by quantitating the formation of UDP-N-acetylmuramyl-l-alanyl-y-d-glutamyl-(l ... [more ▼]

A transpeptidase activity in Escherichia coli was measured independently of other enzymes involved in peptidoglycan synthesis by quantitating the formation of UDP-N-acetylmuramyl-l-alanyl-y-d-glutamyl-(l)-meso-diaminopimelyl-(l)-d-alanyl-[14C]glycine when UDP-N-acetylmuramyl-l-alanyl-y-d-glutamyl-(l)-meso-diaminopimelyl-(l-d-alanyl-d-alanine was used as donor substrate and [14C]glycine as acceptor in a transfer reaction. After extraction of membrane envelopes with Brij-36T and subsequent ammonium sulfate precipitation, DEAE-cellulose chromatography revealed two major fractions; one not adsorbed to the ion-exchange resin and the other adsorbed. The fraction which was bound to DEAE-cellulose was bound to and could be eluted from an ampicillin affinity chromatography system while the fraction not bound to DEAE-cellulose was also not bound to the ampicillin column. Both unbound and bound ampicillin fractions exhibited dd-carboxypeptidase and transpeptidase activities although for equivalent dd-carboxypeptidase activity, the bound ampicillin fraction required about five times more glycine acceptor to achieve the same amount of transpeptidation as the unbound ampicillin fraction. [less ▲]

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See detailFractionation of the reference inoculum of epizootic rabbit enteropathy in discontinuous sucrose gradient identifies aetiological agents in high density fractions
Szalo, Ioan Mihai ULg; Lassence, Cédric ULg; Licois, Dominique et al

in Veterinary Journal (2007), 173(3), 652-657

Epizootic rabbit enteropathy (ERE) is a major cause of economic loss in intensive rabbit production. Since its first recognition in 1997, much work has been done to determine the pathogenic mechanisms of ... [more ▼]

Epizootic rabbit enteropathy (ERE) is a major cause of economic loss in intensive rabbit production. Since its first recognition in 1997, much work has been done to determine the pathogenic mechanisms of the disease and to identify the aetiological agent(s). Unfortunately, the quest for aetiology has only met with limited success despite the ability to reproduce the syndrome by inoculation of intestinal contents from field cases. These intestinal inocula contain a huge number of microorganisms which could all be involved in the aetiology of ERE. To decrease the number of putative agents, the French reference inoculum TEC3 was fractionated on a discontinuous sucrose gradient so that seven fractions (supernatant, 10%, 20%, 30%, 40%, 50% and pellet) were obtained. Specific-pathogen-free rabbits were inoculated with three out of these seven fractions (supernatant, 30%, and pellet). The objectives were: (1) to characterise the seven fractions by bacteriological examination; (2) to verify whether the aetiological agent was present in the fractions by inoculation of rabbits; (3) to assign the aetiological agent of ERE to a morphological group of pathogens; (4) to identify a fraction which could replace the reference inoculum TEC3 in applications such as cell cultures or egg inoculation. The results strongly suggest that ERE is a bacterial disease and does not have a viral or parasitic aetiology. (C) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailFractionation process to produce a new milk ingredient enriched in phospholipids and sphingolipids
Bodson, P.; Dalemans, D.; Danthine, Sabine ULg et al

Poster (2008, October)

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See detailFractionnement de la fumure azotée: deux ou trois fractions? fumure au tallage ou pas?
Bodson, Bernard ULg; Vancutsem, Françoise ULg; Destain, Jean-Pierre ULg et al

in Fumure et protection phytosanitaire des céréales (2004, February 18)

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See detailFractionnement du froment d'hiver : influences variétale et phytotechnique.
Sindic, Marianne ULg; Chevalier, Olivier; Duculot, Jacqueline et al

in Industries des Céréales (1993), (82),

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See detailFractography survey on high cycle fatigue failure: Crack origin characterisation and correlations between mechanical tests and microstructure in Fe-C-Cr-Mo-X alloys
Tchuindjang, Jérôme Tchoufack ULg; Lecomte-Beckers, Jacqueline ULg

in International Journal of Fatigue (2007), 29(4), 713-728

Raw materials were cast from different specific processes in order to produce cleaner steels with a reduced amount of inclusions. Studied materials that are HSS hardened alloys belonging to the Fe-Cr-C-X ... [more ▼]

Raw materials were cast from different specific processes in order to produce cleaner steels with a reduced amount of inclusions. Studied materials that are HSS hardened alloys belonging to the Fe-Cr-C-X system were shared out in four groups depending on the tempering temperature and the presence of secondary and primary carbides. Both inclusions and carbides were roughly assessed by means of Image Analysis. Forging was done on all studied materials with different reduction ratios in order to highlight the texture influence oil mechanical properties. High cycle fatigue tests were made using the boundary method to allow a quick evaluation of results. Fractographic analyses carried out on broken samples led to the definition of four failure modes depending on the nature and the location of the point from which the crack was initiated in one hand, and the roughness of striations in the propagating area over and around the initiation point. Internal and surface crack initiation points were found, the latter being more harmful than the first ones. Though oxides appeared to be more detrimental than other inclusions, primary carbides were also found to be both crack initiation candidates and crack propagation enhancers. Various parameters likely to influence high cycle fatigue failures were finally defined, the most significant one dealing with the nature and location of embedded precipitates and the forging reduction ratio. (c) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailFracture (sociale, numérique etc.)
Vandeninden, Elise ULg

in Durand, Pascal (Ed.) Les nouveaux mots du pouvoir. Abécédaire critique. (2007)

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See detailFracture (sociale, numérique, etc.)
Vandeninden, Elise ULg

in Quaderni : la Revue de la Communication (2007), 63

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See detailFracture and Bone Mineral Density in Hemodialysis Patients
Fontaine, M. A.; Albert, Adelin ULg; Dubois, Bernard ULg et al

in Clinical Nephrology (2000), 54(3), 218-26

AIM: The aim of this cross-sectional study was to determine in hemodialysis patients the pattern of low trauma fracture, the ability of dual X-ray absorptiometry (DXA) to discriminate between patients ... [more ▼]

AIM: The aim of this cross-sectional study was to determine in hemodialysis patients the pattern of low trauma fracture, the ability of dual X-ray absorptiometry (DXA) to discriminate between patients with and without fracture, and the magnitude, distribution and mechanism of bone loss. PATIENTS AND METHODS: Eighty-eight patients were studied. Bone mineral density (BMD) was measured by DXA at lumbar spine (LS), femoral neck (FN) and 3 radius sites (UD, MID and 1/3R). In 11 patients (12.5%), 16 fractures occurred and were predominant at the distal forearm and ribs. RESULTS: Patients with fracture had a significatively lower BMD Z-score at LS (-1.34 +/- 1.66 vs -0.42 +/- 1.23), at FN (-1.58 +/- 1.25 vs -0.60 +/- 1.01), at MID radius (-2.59 +/- 1.34 vs -0.93 +/- 1.76) and 1/3 radius (-1.62 +/- 1.60 vs -0.39 +/- 1.32). They also had a longer history of dialysis (113 +/- 64 vs 53 +/- 65 months). Prevalence of osteoporosis varied from 23% at LS to 50% at MID radius. CONCLUSION: Multiple regression analysis showed that there was no influence of gender, age, parathormone status and primary renal disease on BMD. However, at FN, UD, MID and 1/3 radius, a significantly negative correlation was found between length of dialysis and BMD Z-score. By contrast at LS, there was a positive correlation between age at onset of dialysis and BMD Z-score. Despite occurrence of fracture at the fistula forearm, BMD levels were similar in both arms. [less ▲]

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See detailFracture de la hanche. Données épidémiologiques et approche thérapeutique
Reginster, Jean-Yves ULg

in Le Monde Médical de la Femme (1995), 8

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See detailFracture efficacy of monthly ibandronate and weekly bisphosphonate therapy: a retrospective cohort study
Reginster, Jean-Yves ULg; Blumentals, W.; Barr, C. et al

in Osteoporosis International (2008, April), 19(Suppl.1), 9

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See detailA fracture framework for Euler Bernoulli beams based on a full discontinuous Galerkin formulation/extrinsic cohesive law combination
Becker, Gauthier ULg; Noels, Ludovic ULg

in International Journal for Numerical Methods in Engineering (2011), 85(10), 12271251

A new full Discontinuous Galerkin discretization of Euler Bernoulli beam is presented. The main interest of this framework is its ability to simulate fracture problems by inserting a cohesive zone model ... [more ▼]

A new full Discontinuous Galerkin discretization of Euler Bernoulli beam is presented. The main interest of this framework is its ability to simulate fracture problems by inserting a cohesive zone model in the formulation. With a classical Continuous Galerkin method the use of the cohesive zone model is di cult because as insert a cohesive element between bulk elements is not straightforward. On one hand if the cohesive element is inserted at the beginning of the simulation there is a modification of the structure stiffness and on the other hand inserting the cohesive element during the simulation requires modification of the mesh during computation. These drawbacks are avoided with the presented formulation as the structure is discretized in a stable and consistent way with full discontinuous elements and inserting cohesive elements during the simulation becomes straightforward. A new cohesive law based on the resultant stresses (bending moment and membrane) of the thin structure discretization is also presented. This model allows propagating fracture while avoiding through-the-thickness integration of the cohesive law. Tests are performed to show that the proposed model releases, during the fracture process, an energy quantity equal to the fracture energy for any combination of tension-bending loadings. [less ▲]

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See detailFracture of piezoelectric materials with the X-FEM
Béchet, Eric ULg; Scherzer, Matthias; Kuna, Meinhard

in European Journal of Computational Mechanics (2008), 17/5-7

We present an application of X-FEM to the fracture analysis of piezoelectric materials. These materials are increasingly used in actuators and sensors. Under in service loading, phenomena of crack ... [more ▼]

We present an application of X-FEM to the fracture analysis of piezoelectric materials. These materials are increasingly used in actuators and sensors. Under in service loading, phenomena of crack initiation and propagation may occur due to high electromechanical field concentrations. In the past few years, the extended finite element method (X-FEM) has been applied mostly to model cracks in structural materials. The present paper focuses on the definition of new enrichment functions suitable for cracks in piezolectric structures. The approach is based on specific asymptotic crack tip solutions, derived for piezoelectric materials. [less ▲]

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See detailFracture prevention
Bruyère, Olivier ULg; Reginster, Jean-Yves ULg

in Clinical Evidence (2001), 5

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See detailFracture prevention in osteoporosis: assessing risks, providing solutions.
Reginster, Jean-Yves ULg; Brandi, M. L.

in Osteoporosis International (2010), 21 Suppl 2

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See detailFracture prevention in postmenopausal women
Bruyère, Olivier ULg; Edwards, John; Reginster, Jean-Yves ULg

in Clinical Evidence (2005), 13

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See detailFracture prevention in postmenopausal women
Bruyère, Olivier ULg; Reginster, Jean-Yves ULg

in Clinical Evidence (2001), 6

Detailed reference viewed: 7 (4 ULg)