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We compute cross-correlation Stokes I ... [more ▼]We report on high-resolution spectropolarimetric observations of the hot subdwarf stars HD 76431 and Feige 66, using the ESPaDOnS echelle spectropolarimeter at CFHT. We compute cross-correlation Stokes I and V line profiles to enhance the signal-to-noise ratio. We then average all available cross-correlation profiles of each star to further decrease the noise level. Although both targets were previously reported to host kilo-gauss magnetic fields, we do not derive any evidence of large-scale photospheric fields from our sets of observations, in spite of tight error bars on the longitudinal field of the order of 60 gauss for HD 76431 and 200 gauss for Feige 66. A new analysis of FORS1 observations of HD 76431, which provided the basis for the original claim of field detection, confirms the absence of any detectable Zeeman signature, with an error bar of about 100 gauss on the longitudinal magnetic field. [less ▲]Detailed reference viewed: 20 (2 ULg) High-resolution spectroscopy of the C2 Swan 0-0 band from Comet P/HalleyLambert, D. L.; Sheffer, Y.; Danks, A. C. et alin Astrophysical Journal (1990), 353High-resolution spectra of the C2 Swan system's 0-0 band from Comet P/Halley in March 1986 show that the populations of the upper state's rotational levels may be described by two rotational temperatures ... [more ▼]High-resolution spectra of the C2 Swan system's 0-0 band from Comet P/Halley in March 1986 show that the populations of the upper state's rotational levels may be described by two rotational temperatures. The low rotational levels provide a low temperature, Trot = about 600 - 700 K. The higher levels correspond to about 3200 K. If a contribution from the 3200-K molecules is subtracted from the populations of the low-J-prime levels, the latter are characterized by Trot = about 190 K. A comparison with recent predictions for C2 molecules fluorescing in sunlight shows that the observed and predicted level populations are in good agreement for J-prime greater than about 15, but there is a sharp disagreement for the low rotational levels. [less ▲]Detailed reference viewed: 17 (0 ULg) High-resolution X-ray diagnostics of colliding wind interactions in massive binariesRauw, Grégor ; Nazé, Yaël ; Mahy, Laurent et alPoster (2010)Detailed reference viewed: 6 (3 ULg) High-resolution X-ray microtomography to determine the kinetics of water vapour adsorptionLodewyckx, Peter; Léonard, Angélique ; Blacher, Silvia Conference (2005, October)Detailed reference viewed: 16 (0 ULg) High-resolution X-ray spectroscopy of the magnetic Of?p star HD148937Nazé, Yaël ; Zhekov, S. A.; Walborn, N. R.in Astrophysical Journal (2012), 746High-resolution data of the peculiar magnetic massive star HD148937 were obtained with Chandra-HETGS, and are presented here in combination with a re-analysis of the older XMM-RGS data. The lines of the ... [more ▼]High-resolution data of the peculiar magnetic massive star HD148937 were obtained with Chandra-HETGS, and are presented here in combination with a re-analysis of the older XMM-RGS data. The lines of the high-Z elements (Mg, Si, S) were found to be unshifted and relatively narrow (FWHM of about 800km/s), i.e. narrower than the O line recorded by RGS, which possibly indicates that the hot plasma is multi-thermal and has several origins. These data further indicate a main plasma temperature of about 0.6keV and a formation of the X-ray emission at about one stellar radius above the photosphere. From the spectral fits and the H-to-He line ratios, the presence of very hot plasma is however confirmed, though with a smaller relative strength than for the prototype magnetic oblique rotator $\theta^1$\,Ori\,C. Both stars thus share many similarities, but HD148937 appears less extreme than $\theta^1$\,Ori\,C despite having also a large magnetic confinement parameter. [less ▲]Detailed reference viewed: 177 (1 ULg) High-resolution X-ray spectroscopy of theta CarinaeNazé, Yaël ; Rauw, Grégor in Astronomy and Astrophysics (2008), 490(2), 801-806Context. The peculiar hot star. Car in the open cluster IC2602 is a blue straggler as well as a single-line binary with a short period (2.2d). Aims. Its high-energy properties are not well known, though X ... [more ▼]Context. The peculiar hot star. Car in the open cluster IC2602 is a blue straggler as well as a single-line binary with a short period (2.2d). Aims. Its high-energy properties are not well known, though X-rays can provide useful constraints on the energetic processes at work in binaries as well as in peculiar, single objects. Methods. We present the analysis of a 50 ks exposure taken with the XMM-Newton observatory. It provides medium as well as high-resolution spectroscopy. Results. Our high-resolution spectroscopy analysis reveals a very soft spectrum with multiple temperature components (1-6 MK) and an X-ray flux slightly below the 'canonical' value (log [L-X(0.1-10.)/L-BOL]similar to -7). The X-ray lines appear surprisingly narrow and unshifted, reminiscent of those of beta Cru and tau Sco. Their relative intensities confirm the anomalous abundances detected in the optical domain (C strongly depleted, N strongly enriched, O slightly depleted). In addition, the X-ray data favor a slight depletion in neon and iron, but they are less conclusive for the magnesium abundance, which may be solar-like. While no significant changes occur during the XMM-Newton observation, variability in the X-ray domain is detected in the long-term range. The formation radius of the X-ray emission is loosely constrained to < 5 R-circle dot, which allows for a range of models (wind-shock, corona, magnetic confinement,...) though not all of them can be reconciled with the softness of the spectrum and the narrowness of the lines. [less ▲]Detailed reference viewed: 18 (1 ULg) High-resolution X-ray structures of human apoferritin H-chain mutants correlated with their activity and metal-binding sites.Toussaint, Louise in Journal of Molecular Biology (2007), 365(2), 440-52Ferritins are a family of proteins distributed widely in nature. In bacterial, plant, and animal cells, ferritin appears to serve as a soluble, bioavailable, and non-toxic form of iron provider. Ferritins ... [more ▼]Ferritins are a family of proteins distributed widely in nature. In bacterial, plant, and animal cells, ferritin appears to serve as a soluble, bioavailable, and non-toxic form of iron provider. Ferritins from animal sources are heteropolymers composed of two types of subunit, H and L, which differ mainly by the presence (H) or absence (L) of active ferroxidase centres. We report the crystallographic structures of four human H apoferritin variants at a resolution of up to 1.5 Angstrom. Crystal derivatives using Zn(II) as redox-stable alternative for Fe(II), allows us to characterize the different metal-binding sites. The ferroxidase centre, which is composed of sites A and B, binds metal with a preference for the A site. In addition, distinct Zn(II)-binding sites were found in the 3-fold axes, 4-fold axes and on the cavity surface near the ferroxidase centre. To study the importance of the distance of the two metal atoms in the ferroxidase centre, single and double replacement of glutamate 27 (site A) and glutamate 107 (site B), the two axial ligands, by aspartate residues have been carried out. The consequences for metal binding and the correlation with Fe(II) oxidation rates are discussed. [less ▲]Detailed reference viewed: 1 (0 ULg) High-Resolution, Human-Bovine Comparative Mapping Based on a Closed Yac Contig Spanning the Bovine Mh LocusPirottin, Dimitri ; Poncelet, D.; Grobet, Luc et alin Mammalian Genome : Official Journal of the International Mammalian Genome Society (1999), 10(3), 289-93A closed YAC contig spanning the mh locus was assembled by STS content mapping with seven microsatellite markers, eight genes or EST, and nine STS corresponding to YAC ends. The contig comprises 27 YACs ... [more ▼]A closed YAC contig spanning the mh locus was assembled by STS content mapping with seven microsatellite markers, eight genes or EST, and nine STS corresponding to YAC ends. The contig comprises 27 YACs, has an average depth of 4.3 YACs, and spans an estimated 1.2 Mb. A linkage map was constructed based on five of the microsatellite markers anchored to the contig and shown to span 7 cM, yielding a ratio of 160 kb/1 cM for the corresponding chromosome region. Comparative mapping data indicate that the constructed contig spans an evolutionary breakpoint connecting two chromosome segments that are syntenic but not adjacent in the human. Consolidation of human gene order by means of whole genome radiation hybrids and its comparison with the bovine order as inferred from the contig confirm conservation of gene order within segments. [less ▲]Detailed reference viewed: 30 (6 ULg) High-risk human papillomavirus infection of the genital tract of women with a previous history or current high-grade vulvar intraepithelial neoplasiaGoffin, Frédéric ; Mayrand, Marie-Hélène; Gauthier, Philippe et alin Journal of Medical Virology (2006), 78(6), 814-819Human papillomavirus (HPV) infection is associated with high-grade vulvar intraepithelial neoplasia (VIN-3). The prevalence of anogenital HPV infection in women with previously treated VIN-3 has not been ... [more ▼]Human papillomavirus (HPV) infection is associated with high-grade vulvar intraepithelial neoplasia (VIN-3). The prevalence of anogenital HPV infection in women with previously treated VIN-3 has not been documented yet. This cross-sectional study compared high-risk HPV DNA detection rates in women with past (n = 30) and current (n = 22) VIN-3 to those without current or past VIN (n = 86). HPV DNA was detected in vulvar and cervical samples with Hybrid Capture 2 (HC-2). Smoking was associated in multivariate analysis with current VIN-3 (odds ratio (OR) 8.3, 95% confidence interval (CI) 2.0-8.2) and any VIN-3 history (OR 6.5, 95% CI 2.5-16.5). High-risk HPV DNA was found on the vulva of 64%, 33%, and 20% of women with current VIN-3, past VIN-3, and without previous or current VIN, respectively. After controlling for age and smoking, high-risk HPV vulvar infection was associated with cervical high-risk HPV infection (OR 8.6, 95% CI 2.8-26.5; P = 0.001). After controlling for age, HPV infection was more often multifocal in women with current VIN-3 compared to women with previous but no current VIN-3 lesion (OR 17.6, 95% CI 1.4-227.2). Multifocal vulvar HPV infection was detected in women with previous or active VIN-3. Longitudinal studies are required to determine if the multifocality of HPV infection on the vulva could explain the high recurrence rate of VIN-3. [less ▲]Detailed reference viewed: 1 (0 ULg) High-sensitivity staining of proteins for one- and two-dimensional gel electrophoresis using post migration covalent staining with a ruthenium fluorophore.Tokarski, Caroline; Cren-Olive, Cecile; Fillet, Marianne et alin Electrophoresis (2006), 27(7), 1407-16This paper describes the use of a ruthenium complex ((bis(2,2'-bipyridine)-4'-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophosphate), abbreviated below as ASCQ_Ru) commercially ... [more ▼]This paper describes the use of a ruthenium complex ((bis(2,2'-bipyridine)-4'-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophosphate), abbreviated below as ASCQ_Ru) commercially available and chemically pure. This new ruthenium complex ASCQ_Ru brings an activated ester, allowing the selective acylation of amino acid side chain amines for the post migration staining of proteins separated in 1-DE and 2-DE. The protocol used is a simple three-step protocol fixing the proteins in the gel, staining and then washing, as no lengthy destaining step is required. First the critical staining step was optimized. Although in solution the best described pH for acylating proteins with this reagent is phosphate buffer at pH 7.0, we found that best medium for in-gel staining is unbuffered ACN/water solution (20/80 v/v). The two other steps are less critical and classical conditions are satisfactory: fixing with 7% acetic acid/10% ethanol solution and washing four times for 10 min with water. Sensitivity tests were performed using 1-DE on protein molecular weight markers. We obtained a higher sensitivity than SYPRO Ruby with a detection limit of 80 pg of protein per well. However, contrary to SYPRO Ruby, ASCQ_Ru exhibits a logarithmic dependency on the amount of protein. The dynamic range is similar to SYPRO Ruby and is estimated between three and four orders of magnitude. Finally, the efficiency of the post migration ASCQ_Ru staining for 2-D gel separation is demonstrated on the whole protein extract from human colon carcinoma cells lines HCT 116. ASCQ_Ru gave the highest number of spot detected compared to other common stains Colloidal CBB, SYPRO Ruby and Deep Purple. [less ▲]Detailed reference viewed: 29 (0 ULg) High-speed imaging use to predict spray retention on barley leavesBoukhalfa, Hassina dite Hafida ; Massinon, Mathieu ; Lebeau, Frédéric in Communications in Agricultural and Applied Biological Sciences (in press)Laboratory studies were conducted to validate the effectiveness of the use of high-speed imaging method to replace chemical nalysis by fluoremetrie. Measurements were performed with a high-speed camera ... [more ▼]Laboratory studies were conducted to validate the effectiveness of the use of high-speed imaging method to replace chemical nalysis by fluoremetrie. Measurements were performed with a high-speed camera coupled with a retro-LED lighting. Size and velocity of the drop were extracted by image analysis. Drop impact types were determined by the operator. Drops were produced with a flat-fan nozzle mounted on a movable ramp. Two surfactants (Break-Thru® S240 and Li700 ®) were sprayed on BBCH 12 barley leaves to highlight the effect of the reduction of surface tension. Relative volume proportions were computed within of an energy scale divided into 11 classes based on the Weber number. results are compared to the results of the chemical analysis by spectrofluerometry. [less ▲]Detailed reference viewed: 60 (20 ULg) High-Technology Service Innovation Success: A Decision-Making PerspectiveVan Riel, Allard ; Lemmink, Jos; Ouwersloot, Hansin Journal of Product Innovation Management (2004), 21(5), 348-359Detailed reference viewed: 48 (2 ULg) High-temperature polyimide nanofoams for microelectronic applicationsHedrick, James L; Carter, K. R.; Cha, J. E. et alin Reactive & Functional Polymers (1996), 30(1-3), 43-53Foamed polyimides have been developed in order to obtain thin film dielectric layers with very low dielectric constants for use in microelectronic devices. In these systems the pore sizes are in the ... [more ▼]Foamed polyimides have been developed in order to obtain thin film dielectric layers with very low dielectric constants for use in microelectronic devices. In these systems the pore sizes are in the nanometer range, thus, the term 'nanofoam'. The polyimide foams are prepared from block copolymers consisting of thermally stable and thermally labile blocks, the latter being the dispersed phase. Foam formation is effected by thermolysis of the thermally labile block, leaving pores of the size and shape corresponding to the initial copolymer morphology. Nanofoams prepared from a number of polyimides as matrix materials were investigated as well as from a number of thermally labile polymers. The foams were characterized by a variety of experiments including TEM, SAXS, WAXD, DMTA, density measurements. refractive index measurements and dielectric constant measurements. Thin film foams, with high thermal stability and low dielectric constants approaching 2.0, can be prepared using the copolymer/nanofoam approach. [less ▲]Detailed reference viewed: 47 (3 ULg) High-throughput analysis of human serum for selected polychlorinated biphenyls (PCBs) by gas chromatography-isotope dilution time-of-flight mass spectrometry (GC-IDTOFMS)Focant, Jean-François ; Cochran, Jack W.; Dimandja, Jean-Marie D. et alin Analyst (2004), 129(4), 331-336A method for the high-throughput analysis of human serum for the 38 most prevalent polychlorinated biphenyls (PCBs) based on the use of fast gas chromatography-isotopic dilution time-of-flight mass ... [more ▼]A method for the high-throughput analysis of human serum for the 38 most prevalent polychlorinated biphenyls (PCBs) based on the use of fast gas chromatography-isotopic dilution time-of-flight mass spectrometry (GC-IDTOFMS) is presented. The chromatographic separation time was 8 min. The separation of the congeners was carried out either chromatographically or analytically using the mass spectral deconvolution capability of the TOFMS. The instrument and the method limits of detection (LODs) were 0.5 pg muL(-1) and 20 pg muL(-1), respectively, which is not as good as the one achieved using high resolution mass spectrometry (HRMS) but allows the detection and quantification of the prevalent PCBs present in real human serum samples. The dynamic range covered 3 orders of magnitude. The comparison with the high resolution mass spectrometry (HRMS) reference method (28 min) was good and some separation improvements have been observed. This method allows the analysis of 100 samples per day per instrument. [less ▲]Detailed reference viewed: 21 (3 ULg) High-throughput biomonitoring of dioxins and polychlorinated biphenyls at the sub-picogram level in human serumFocant, Jean-François ; Eppe, Gauthier ; Massart, Anne-Cécile et alin Journal of Chromatography. A (2006), 1130(1), 97-107We report on the use of a state-of-the-art method for the measurement of selected polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and polychlorinated biphenyls in human serum specimens ... [more ▼]We report on the use of a state-of-the-art method for the measurement of selected polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and polychlorinated biphenyls in human serum specimens. The sample preparation procedure is based on manual small size solid-phase extraction (SPE) followed by automated clean-up and fractionation using multi-sorbent liquid chromatography columns. SPE cartridges and all clean-up columns are disposable. Samples are processed in batches of 20 units, including one blank control (BC) sample and one quality control (QC) sample. The analytical measurement is performed using gas chromatography coupled to isotope dilution high-resolution mass spectrometry. The sample throughput corresponds to one series of 20 samples per day, from sample reception to data quality cross-check and reporting, once the procedure has been started and series of samples keep being produced. Four analysts are required to ensure proper performances of the procedure. The entire procedure has been validated under International Organization for Standardization (ISO) 17025 criteria and further tested over more than 1500 unknown samples during various epidemiological studies. The method is further discussed in terms of reproducibility, efficiency and long-term stability regarding the 35 target analytes. Data related to quality control and limit of quantification (LOQ) calculations are also presented and discussed. (c) 2006 Elsevier B.V. All rights reserved. [less ▲]Detailed reference viewed: 54 (10 ULg) High-throughput method for comparative analysis of denaturing gradient gel electrophoresis profiles from human fecal samples reveals significant increases in two bifidobacterial species after inulin-type prebiotic intake.Joossens, Marie; Huys, Geert; Van Steen, Kristel et alin FEMS Microbiology Ecology (2011), 75(2), 343-9Denaturing gradient gel electrophoresis (DGGE) is one of the most commonly used molecular tools to study complex microbial communities. Despite its widespread use, meaningful interpretative analysis ... [more ▼]Denaturing gradient gel electrophoresis (DGGE) is one of the most commonly used molecular tools to study complex microbial communities. Despite its widespread use, meaningful interpretative analysis remains a major drawback of this method. We evaluated the combination of computer-assisted band-matching with nonparametric statistics for comparative analysis of DGGE banding patterns. Fecal samples from 17 healthy volunteers who consumed 20 g of the prebiotic compound oligofructose-enriched inulin (OF-IN) for 4 weeks were analyzed before and after treatment. DGGE fingerprinting profiles were analyzed using bionumerics software version 4.6., which resulted in a data matrix that was used for statistical analysis. When comparing DGGE profiles before and after OF-IN intake with a Wilcoxon nonparametric test for paired data, two band-classes increased significantly after OF-IN intake (P<0.003 and <0.02). These two band-classes could be assigned to the species Bifidobacterium longum and Bifidobacterium adolescentis by band-sequencing analysis, and their significant increase was quantitatively confirmed with real-time PCR using species-specific primers (respectively P<0.012 and <0.010). Therefore, the nonparametric analysis of a data matrix obtained by computer-assisted band-matching of complex profiles facilitated the interpretative analysis of these profiles and provided an objective and high-throughput method for the detection of significant taxonomic differences in larger numbers of complex profiles. [less ▲]Detailed reference viewed: 34 (4 ULg) High-throughput methodology applied to screening of carbohydrate-based surfactant interfacial properties for colloidal system formulationsRazafindralambo, Hary ; Blecker, Christophe ; Deroanne et alPoster (2009)Carbohydrate-based surfactants (CBS) constitute an attractive class of amphiphilic molecules owing to their structural diversity generating a wide range of properties which could be developed in food and ... [more ▼]Carbohydrate-based surfactants (CBS) constitute an attractive class of amphiphilic molecules owing to their structural diversity generating a wide range of properties which could be developed in food and non-food applications. Based on many functional groups of the carbohydrate part, it is possible to design multiple amphiphilic structures of CBS varying in the hydrophilic head groups (mono-, oligo-, or polysaccharides), hydrophobic tail (mono-, di-, tricatenar) but also in the linker/spacer between them. The main structures of CBS include mono- and bicatenar glycolipids, bolaforms, and gemini. Moreover, CBS compounds can be produced from the most abundant renewable materials allowing large product concept possibilities. Among general properties of surfactants, interfacial properties molecules occupy a fundamental key role for colloidal system formulations since they control most of technological aptitudes required for forming and stabilizing food, cosmetic, agrochemical, detergent, and pharmaceutical products. High-throughput methodology applied to screening of CBS interfacial properties appears crucial for achieving optimum formulations of colloidal systems like foams and emulsions for which small amounts of single or mix surface-active agents are often needed and numerous physical and chemical parameters are involved. This general approach may be applied to any amphiphilic molecules produced from other renewable resources of surface-active compounds like micro-organisms and by direct extraction from vegetable materials. One of the key factors for the development of this methodology is the use of automated instrument systems in laboratory scale which are still lacking in this field compared to those existing in the analytical chemistry and biochemistry areas. [less ▲]Detailed reference viewed: 26 (7 ULg) High-yield radiosynthesis and preliminary in vivo evaluation of p-[18F]MPPF, a fluoro analog of WAY-100635.Le Bars, Didier; Lemaire, Christian ; Ginovart, N. et alin Nuclear Medicine & Biology (1998), 25(4), 343-50No-carrier-added 4-[18F]fluoro-N-[2-[1-(2-methoxyphenyl)-1 piperazinyl]ethyl-N-2-pyridinyl-benzamide (p-[18F]MPPF) was synthesized by nucleophilic substitution of the corresponding nitro compound in the ... [more ▼]No-carrier-added 4-[18F]fluoro-N-[2-[1-(2-methoxyphenyl)-1 piperazinyl]ethyl-N-2-pyridinyl-benzamide (p-[18F]MPPF) was synthesized by nucleophilic substitution of the corresponding nitro compound in the presence of Kryptofix 222 and K2CO3 by microwave heating (3 min, 500 W) using a remotely controlled radiosynthesis. Baseline separation of p-[18F]MPPF from the nitro derivative was performed on a semipreparative HPLC C18 column. After Sep-Pak formulation, the radiopharmaceutical was obtained with a radiochemical yield of 25% (EOS) in about 70 min. Specific radioactivity averaged between 1-5 Ci/micromol EOS. Labelling of the ortho and meta derivatives was also attempted. Brain uptake of p-[18F]MPPF was studied with PET on fluothane-anesthetized cats. Following intravenous injection of p-[18F]MPPF, high accumulation of radioactivity was observed in the hippocampus and cerebral cortex. Low levels of radioactivity were observed in cerebellum. At 30 min, the mean hippocampus/cerebellum and cortex/cerebellum ratios were 5 and 3.8, respectively. The accumulation of the tracer was blocked by prior administration of reference WAY-100635, demonstrating the specificity of the ligand. [less ▲]Detailed reference viewed: 41 (6 ULg) High-Yield Synthesis of a (3H)Ethylenediamine Ditetrodotoxin DerivativeBontemps, José; Cantineau, Robert; Grandfils, Christian et alin Analytical Biochemistry (1984), 139Detailed reference viewed: 9 (2 ULg) High-Yield Synthesis Of The Bovine Leukemia-Virus (Blv) P24 Major Internal Protein In Saccharomyces-CerevisiaeDumont, Jacques; Legrain, M.; Portetelle, Daniel et alin Gene (1989), 79(2), Bovine leukemia virus (BLV) p24 gene was expressed in Saccharomyces cerevisiae under the control of the PHO5 (encoding repressible acid phosphatase, rAPase) promoter. Yeast cells were transformed by a ... [more ▼]Bovine leukemia virus (BLV) p24 gene was expressed in Saccharomyces cerevisiae under the control of the PHO5 (encoding repressible acid phosphatase, rAPase) promoter. Yeast cells were transformed by a yeast-E. coli shuttle vector carrying the PHO5 promoter, the p24 gene and the CYC1 transcription terminator. After low inorganic phosphate (Pi) induction of the PHO5 promoter, p24 accumulated in the producing cells up to a concentration representing 10% of total soluble proteins. The expression level of p24 gene was not increased by insertion of the positive regulatory gene PHO4 on the p24 expression vector. The p24 produced in this system and incubated in crude yeast extract showed a remarkably high resistance to proteolytic degradation, a feature that presumably correlates with the compact globular conformation of the protein combined to the stabilizing effect of the N-terminal residue. [less ▲]Detailed reference viewed: 7 (0 ULg)