Browsing Complete repository Arts & humanities   Archaeology   Art & art history   Classical & oriental studies   History   Languages & linguistics   Literature   Performing arts   Philosophy & ethics   Religion & theology   Multidisciplinary, general & others Business & economic sciences   Accounting & auditing   Production, distribution & supply chain management   Finance   General management & organizational theory   Human resources management   Management information systems   Marketing   Strategy & innovation   Quantitative methods in economics & management   General economics & history of economic thought   International economics   Macroeconomics & monetary economics   Microeconomics   Economic systems & public economics   Social economics   Special economic topics (health, labor, transportation…)   Multidisciplinary, general & others Engineering, computing & technology   Aerospace & aeronautics engineering   Architecture   Chemical engineering   Civil engineering   Computer science   Electrical & electronics engineering   Energy   Geological, petroleum & mining engineering   Materials science & engineering   Mechanical engineering   Multidisciplinary, general & others Human health sciences   Alternative medicine   Anesthesia & intensive care   Cardiovascular & respiratory systems   Dentistry & oral medicine   Dermatology   Endocrinology, metabolism & nutrition   Forensic medicine   Gastroenterology & hepatology   General & internal medicine   Geriatrics   Hematology   Immunology & infectious disease   Laboratory medicine & medical technology   Neurology   Oncology   Ophthalmology   Orthopedics, rehabilitation & sports medicine   Otolaryngology   Pediatrics   Pharmacy, pharmacology & toxicology   Psychiatry   Public health, health care sciences & services   Radiology, nuclear medicine & imaging   Reproductive medicine (gynecology, andrology, obstetrics)   Rheumatology   Surgery   Urology & nephrology   Multidisciplinary, general & others Law, criminology & political science   Civil law   Criminal law & procedure   Criminology   Economic & commercial law   European & international law   Judicial law   Metalaw, Roman law, history of law & comparative law   Political science, public administration & international relations   Public law   Social law   Tax law   Multidisciplinary, general & others Life sciences   Agriculture & agronomy   Anatomy (cytology, histology, embryology...) & physiology   Animal production & animal husbandry   Aquatic sciences & oceanology   Biochemistry, biophysics & molecular biology   Biotechnology   Entomology & pest control   Environmental sciences & ecology   Food science   Genetics & genetic processes   Microbiology   Phytobiology (plant sciences, forestry, mycology...)   Veterinary medicine & animal health   Zoology   Multidisciplinary, general & others Physical, chemical, mathematical & earth Sciences   Chemistry   Earth sciences & physical geography   Mathematics   Physics   Space science, astronomy & astrophysics   Multidisciplinary, general & others Social & behavioral sciences, psychology   Animal psychology, ethology & psychobiology   Anthropology   Communication & mass media   Education & instruction   Human geography & demography   Library & information sciences   Neurosciences & behavior   Regional & inter-regional studies   Social work & social policy   Sociology & social sciences   Social, industrial & organizational psychology   Theoretical & cognitive psychology   Treatment & clinical psychology   Multidisciplinary, general & others      by title 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z or enter first few letters:    OK Showing results 45181 to 45200 of 102194     2255 2256 2257 2258 2259 2260 2261 2262 2263 2264 2265     High-resolution measurements of an upwelling filament during the CAIBEX surveyTroupin, Charles ; Sangrà, Pablo; Arístegui, Javier et alPoster (2011, May 02)Upwelling filaments have been studied for several years. We aim to explain the general process in terms of potential vorticity: An injection of positive vorticity north of the Cape forces the jet to turn ... [more ▼]Upwelling filaments have been studied for several years. We aim to explain the general process in terms of potential vorticity: An injection of positive vorticity north of the Cape forces the jet to turn offshore, in virtue of the principle of vorticity conservation. The Regional Ocean Modeling System (ROMS) is implemented in the Cape Ghir region, with horizontal resolution lower than 1 km. A set of process-oriented experiments has been designed in order to determine the mechanisms at the origin of the filament. [less ▲]Detailed reference viewed: 25 (0 ULg) High-Resolution Microfocus X-Ray Computed Tomography for 3D Surface Roughness Measurements of Additive Manufactured Porous MaterialsKerckhofs, Greet ; Pyka, Grzegorz; Moesen, Maarten et alin ADVANCED ENGINEERING MATERIALS (2013), 15(3), 153-158Since commercially available profiling systems fail when determining the surface roughness of porous materials, we propose a novel protocol for surface roughness quantification of 3D additive manufactured ... [more ▼]Since commercially available profiling systems fail when determining the surface roughness of porous materials, we propose a novel protocol for surface roughness quantification of 3D additive manufactured porous structures based on high-resolution microfocus X-ray computed tomography (micro-CT) images. It allows to non-destructively assess the roughness of these porous materials at the outer surface as well as inside the structure. The noise in the images and the spatial image resolution both have a significant effect on the accuracy of the micro-CT-based roughness measurements. Comparing the roughness parameters of flat substrates determined both with commercially available (optical and contact) profiling systems and the micro-CT-based roughness measurement protocol shows that micro-CT can be applied accurately and in a robust manner for surface roughness quantification of 3D additive manufactured porous materials with a micro-scale roughness. Depending on the dimensions of the roughness, the micro-CT acquisition parameters, i.e., frame averaging and spatial image resolution, need to be fine-tuned. Submicron-scale roughness can currently not be quantified. [less ▲]Detailed reference viewed: 7 (3 ULg) High-resolution modelling of the Antarctic surface mass balance, application for the 20th, 21st and 22nd centuriesAgosta, Cécile ; Favier, Vincent; Krinner, Gerhard et alPoster (2013, April)Although areas below 2000 m above sea level (a.s.l.) cover 40% of the Antarctic grounded ice-sheet, they represent about 75% of the surface mass balance (SMB) of the continent. Because the topography is ... [more ▼]Although areas below 2000 m above sea level (a.s.l.) cover 40% of the Antarctic grounded ice-sheet, they represent about 75% of the surface mass balance (SMB) of the continent. Because the topography is complex in many of these regions, SMB modelling is highly dependent on resolution, and studying the impact of Antarctica on the fu- ture rise in sea level requires high resolution physical approaches. We have developed a new, low time consuming, physical downscaling model for high-resolution (15 km) long-term SMB projections. Here, we present results of our SMHiL (surface mass balance high-resolution downscaling) model, which was forced with the LMDZ4 atmo- spheric general circulation model to assess SMB variation in the 21st and the 22nd centuries under two different scenarios. The higher resolution of SMHiL reproduces the geographical patterns of SMB better and induces a significantly higher averaged SMB over the grounded ice-sheet for the end of the 20th century. Our comparison of more than 2700 quality-controlled field data showed that LMDZ4 and SMHiL fit the observed values equally well. Never- theless, field data below 2000 m a.s.l. are too scarce to settle SMHiL efficiency. Measuring the SMB in these undocumented areas is a future scientific priority. Our results suggest that running LMDZ4 at a finer resolution may give a future increase in SMB in Antarctica between 15% to 30% higher than its standard resolution. Future changes in the Antarctic SMB at low elevations will result from the conflict between higher snow accumulation and runoff. For this reason, developing a downscaling model was crucial to represent processes in sufficient detail and correctly model the SMB in coastal areas. [less ▲]Detailed reference viewed: 22 (3 ULg) High-resolution modelling of the Antarctic surface mass balance, application for the 20th, 21st and 22nd centuriesAgosta, Cécile ; Favier, Vincent; Krinner, Gerhard et alE-print/Working paper (2013)About 75% of the Antarctic surface mass gain occurs over areas below 2000 m asl, which cover 40% of the grounded ice-sheet. As the topography is complex in many of these regions, SMB modelling is highly ... [more ▼]About 75% of the Antarctic surface mass gain occurs over areas below 2000 m asl, which cover 40% of the grounded ice-sheet. As the topography is complex in many of these regions, SMB modelling is highly dependent on resolution, and studying the impact of Antarctica on the future rise in sea level requires physical approaches. We have developed a low time consuming, physical downscaling model for high-resolution (15 km) long-term surface mass balance (SMB) projections. Here, we present results of this model, called SMHiL (surface mass balance high-resolution downscaling), which was forced with the LMDZ4 atmospheric general circulation model to assess SMB variation in the 21st and the 22nd centuries under two different scenarios. The higher resolution of SMHiL better reproduces the geographical patterns of SMB and increase significantly the averaged SMB over the grounded ice-sheet for the end of the 20th century. A comparison with more than 3200 quality-controlled field data shows that LMDZ4 and SMHiL compare the observed values equally well. Nevertheless, field data below 2000 m asl are too scarce to efficiency show the interest of SMHiL and measuring the SMB in these undocumented areas should be then a future scientific priority. Our results suggest that running LMDZ4 at a finer resolution (15km) may give a future increase in SMB in Antarctica about 30% higher than by using its standard resolution (60 km) due to higher increase in precipitation in the coastal areas at 15 km. However, a part (~ 15%) of these discrepancies could be an artefact from SMHiL since it neglects the foehn effect and then likely overestimates the precipitation increase. Future changes in the Antarctic SMB at low elevations will result from the conflict between higher snow accumulation and runoff. For this reason, developing downscaling models is crucial to represent processes in sufficient detail and correctly model the SMB in the coastal areas. [less ▲]Detailed reference viewed: 43 (5 ULg) High-resolution of trace elements (Mg, U, Sr, Ba and Zn) in speleothems as Holocene palaeoclimatic proxies: Père Noël cave, BelgiumAllan, Mouhamd ; Verheyden, Sophie; Riotte et alPoster (2013, April 08)Detailed reference viewed: 39 (16 ULg) High-Resolution Optical and Near-Infrared Imaging of the Quadruple Quasar RX J0911.4+0551Burud, I.; Courbin, F.; Lidman, C. et alin Astrophysical Journal (1998), 501We report the detection of four images in the recently discovered lensed QSO RX J0911.4+0551. With a maximum angular separation of 3."1, it is the quadruply imaged QSO with the widest known angular ... [more ▼]We report the detection of four images in the recently discovered lensed QSO RX J0911.4+0551. With a maximum angular separation of 3."1, it is the quadruply imaged QSO with the widest known angular separation. Raw and deconvolved data reveal an elongated lens galaxy. The observed reddening in at least two of the four QSO images suggests differential extinction by this lensing galaxy. We show that both an ellipticity of the galaxy ( epsilon _{{min}}=0.075 ) and an external shear ( gamma _{{min}}=0.15 ) from a nearby mass have to be included in the lensing potential in order to reproduce the complex geometry observed in RX J0911.4+0551. A possible galaxy cluster is detected about 38" from RX J0911.4+0551 and could contribute to the X-ray emission observed by ROSAT in this field. The color of these galaxies indicates a plausible redshift in the range of 0.6-0.8. [less ▲]Detailed reference viewed: 13 (2 ULg) High-resolution optical spectroscopy of Plaskett's starLinder, Natacha ; Rauw, Grégor ; Martins, Fabrice et alin Astronomy and Astrophysics (2008), 489(2), 713-723Context. Plaskett's star (HD 47 129) is a very massive O + O binary that belongs to the Mon OB2 association. Previous work suggests that this system displays the Struve-Sahade effect although the ... [more ▼]Context. Plaskett's star (HD 47 129) is a very massive O + O binary that belongs to the Mon OB2 association. Previous work suggests that this system displays the Struve-Sahade effect although the measurements of the secondary radial velocities are very difficult and give controversial results. Both components have powerful stellar winds that collide and produce a strong X-ray emission. Aims. Our aim is to study the physical parameters of this system in detail and to investigate the relation between its spectral properties and its evolutionary status. Methods. We present here analysis of an extensive set of high-resolution optical spectra of HD 47 129. We used a disentangling method to separate the individual spectra of each star. We derived a new orbital solution and discuss the spectral classification of both components. A Doppler tomography technique applied to the emission lines H alpha and He II gamma 4686 yields a Doppler map that illustrates the wind interactions in the system. Finally, an atmosphere code is used to determine the different chemical abundances of the system components and the wind parameters. Results. HD 47 129 appears to be an O8 III/I + O7.5 III binary system in a post RLOF evolutionary stage, where matter has been transferred from the primary to the secondary star. The He overabundance of the secondary supports this scenario. In addition, the N overabundance and C underabundance of the primary component confirm previous results based on X-ray spectroscopy and indicate that the primary is an evolved massive star. We also determined a new orbital solution, with M-P sin(3) i = 45.4 +/- 2.4 M-circle dot and M-S sin(3) i = 47.3 +/- 0.3 M-circle dot. Furthermore, the secondary star has a high rotational velocity (nu sin i similar to 300 km s(-1)) that deforms its surface, leading to a non-uniform distribution in effective temperature. This could explain the variations in the equivalent widths of the secondary lines with phase. We suggest that the wind of the secondary star is confined near the equatorial plane because of its high rotational velocity, affecting the ram pressure equilibrium in the wind interaction zone. [less ▲]Detailed reference viewed: 34 (4 ULg) High-resolution reconstruction of atmospheric deposition of trace metals and metalloids since AD 1400 recorded by ombrotrophic peat cores in Hautes-Fagnes, BelgiumAllan, Mouhamd ; le roux, gael; De Vleeschouwer, François et alin Environmental Pollution (2013), 1The objective of our study was to determine the trace metal accumulation rates in the Misten bog, Hautes-Fagnes, Belgium, and assess these in relation to established histories of atmospheric emissions ... [more ▼]The objective of our study was to determine the trace metal accumulation rates in the Misten bog, Hautes-Fagnes, Belgium, and assess these in relation to established histories of atmospheric emissions from anthropogenic sources. To address these aims we analyzed trace metals and metalloids (Pb, Cu, Ni, As, Sb, Cr, Co, V, Cd and Zn), as well as Pb isotopes, using XRF, Q-ICP-MS and MC-ICP-MS, respectively in two 40-cm peat sections, spanning the last 600 yr. The temporal increase of metal ﬂuxes from the inception of the Industrial Revolution to the present varies by a factor of 5 to 50, with peak values found between AD 1930 and 1990. A cluster analysis combined with Pb isotopic composition allows the identification of the main sources of Pb and by inference of the other metals, which indicates that coal consumption and metallurgical activities were the predominant sources of pollution during the last 600 years. [less ▲]Detailed reference viewed: 117 (38 ULg) High-resolution respirometry to assess muscle mitochondrial respiratory function in horses: potential applications in sport and myopathic horsesVotion, Dominique ; Gnaiger, Erich; Lemieux, Hélène et alin In Proceedings: 3rd MiPsummer School, Baton Rouge, USA (2009, June)Detailed reference viewed: 10 (3 ULg) HIGH-RESOLUTION SPECTRA OF JUPITER NORTHERN AURORAL ULTRAVIOLET EMISSION WITH THE HUBBLE-SPACE-TELESCOPETRAFTON, L. M.; Gérard, Jean-Claude ; Munhoven, Guy et alin Astrophysical Journal (1994), 421(2), 816-827The first spectroscopic observations of planetary aurora with the HST are reported. These include spectral regions centered on the H-2 Lyman and Werner bands of a region of Jupiter's northern aurora. The ... [more ▼]The first spectroscopic observations of planetary aurora with the HST are reported. These include spectral regions centered on the H-2 Lyman and Werner bands of a region of Jupiter's northern aurora. The observations were made with the Goddard High Resolution Spectrograph (GHRS) using the Large Science Aperture as part of a campaign to study Jupiter at the time of the Ulysses flyby. The individual rotational-vibrational bands are resolved and the observed emissions are essentially all from H-2. A rotational-vibrational temperature for H-2 of 530 +/- 100 K is derived, a value significantly less than the 850-1100 K reported for Jovian H-3(+) in the near-infrared but consistent with the temperature reported for fundamental-band quadrupole H-2 emission. Comparison with the Faint Object Camera (FOC) images shows that the observed region was not one of the hot spots of the aurora. The results are interpreted in terms of electron impact excitation of H-2 from secondary particles generated by primaries precipitating into Jupiter's atmosphere from the magnetosphere. In the region of the aurora observed, the homopause level is found to be significantly hotter but not necessarily higher than observed at nonauroral latitudes. The equatorial H-2 dayglow spectrum was also detected; its intensity was 3.2 kR or 13% of the strength of the observed auroral emission. [less ▲]Detailed reference viewed: 21 (3 ULg) High-resolution spectropolarimetric observations of hot subdwarfsPetit, P.; Van Grootel, Valérie ; Bagnulo, S. et alin Leaflet - Astronomical Society of the Pacific (2012, March 01), 452We report on high-resolution spectropolarimetric observations of the hot subdwarf stars HD 76431 and Feige 66, using the ESPaDOnS echelle spectropolarimeter at CFHT. We compute cross-correlation Stokes I ... [more ▼]We report on high-resolution spectropolarimetric observations of the hot subdwarf stars HD 76431 and Feige 66, using the ESPaDOnS echelle spectropolarimeter at CFHT. We compute cross-correlation Stokes I and V line profiles to enhance the signal-to-noise ratio. We then average all available cross-correlation profiles of each star to further decrease the noise level. Although both targets were previously reported to host kilo-gauss magnetic fields, we do not derive any evidence of large-scale photospheric fields from our sets of observations, in spite of tight error bars on the longitudinal field of the order of 60 gauss for HD 76431 and 200 gauss for Feige 66. A new analysis of FORS1 observations of HD 76431, which provided the basis for the original claim of field detection, confirms the absence of any detectable Zeeman signature, with an error bar of about 100 gauss on the longitudinal magnetic field. [less ▲]Detailed reference viewed: 20 (2 ULg) High-resolution spectroscopy of the C2 Swan 0-0 band from Comet P/HalleyLambert, D. L.; Sheffer, Y.; Danks, A. C. et alin Astrophysical Journal (1990), 353High-resolution spectra of the C2 Swan system's 0-0 band from Comet P/Halley in March 1986 show that the populations of the upper state's rotational levels may be described by two rotational temperatures ... [more ▼]High-resolution spectra of the C2 Swan system's 0-0 band from Comet P/Halley in March 1986 show that the populations of the upper state's rotational levels may be described by two rotational temperatures. The low rotational levels provide a low temperature, Trot = about 600 - 700 K. The higher levels correspond to about 3200 K. If a contribution from the 3200-K molecules is subtracted from the populations of the low-J-prime levels, the latter are characterized by Trot = about 190 K. A comparison with recent predictions for C2 molecules fluorescing in sunlight shows that the observed and predicted level populations are in good agreement for J-prime greater than about 15, but there is a sharp disagreement for the low rotational levels. [less ▲]Detailed reference viewed: 17 (0 ULg) High-resolution X-ray diagnostics of colliding wind interactions in massive binariesRauw, Grégor ; Nazé, Yaël ; Mahy, Laurent et alPoster (2010)Detailed reference viewed: 6 (3 ULg) High-resolution X-ray microtomography to determine the kinetics of water vapour adsorptionLodewyckx, Peter; Léonard, Angélique ; Blacher, Silvia Conference (2005, October)Detailed reference viewed: 16 (0 ULg) High-resolution X-ray spectroscopy of the magnetic Of?p star HD148937Nazé, Yaël ; Zhekov, S. A.; Walborn, N. R.in Astrophysical Journal (2012), 746High-resolution data of the peculiar magnetic massive star HD148937 were obtained with Chandra-HETGS, and are presented here in combination with a re-analysis of the older XMM-RGS data. The lines of the ... [more ▼]High-resolution data of the peculiar magnetic massive star HD148937 were obtained with Chandra-HETGS, and are presented here in combination with a re-analysis of the older XMM-RGS data. The lines of the high-Z elements (Mg, Si, S) were found to be unshifted and relatively narrow (FWHM of about 800km/s), i.e. narrower than the O line recorded by RGS, which possibly indicates that the hot plasma is multi-thermal and has several origins. These data further indicate a main plasma temperature of about 0.6keV and a formation of the X-ray emission at about one stellar radius above the photosphere. From the spectral fits and the H-to-He line ratios, the presence of very hot plasma is however confirmed, though with a smaller relative strength than for the prototype magnetic oblique rotator $\theta^1$\,Ori\,C. Both stars thus share many similarities, but HD148937 appears less extreme than $\theta^1$\,Ori\,C despite having also a large magnetic confinement parameter. [less ▲]Detailed reference viewed: 177 (1 ULg) High-resolution X-ray spectroscopy of theta CarinaeNazé, Yaël ; Rauw, Grégor in Astronomy and Astrophysics (2008), 490(2), 801-806Context. The peculiar hot star. Car in the open cluster IC2602 is a blue straggler as well as a single-line binary with a short period (2.2d). Aims. Its high-energy properties are not well known, though X ... [more ▼]Context. The peculiar hot star. Car in the open cluster IC2602 is a blue straggler as well as a single-line binary with a short period (2.2d). Aims. Its high-energy properties are not well known, though X-rays can provide useful constraints on the energetic processes at work in binaries as well as in peculiar, single objects. Methods. We present the analysis of a 50 ks exposure taken with the XMM-Newton observatory. It provides medium as well as high-resolution spectroscopy. Results. Our high-resolution spectroscopy analysis reveals a very soft spectrum with multiple temperature components (1-6 MK) and an X-ray flux slightly below the 'canonical' value (log [L-X(0.1-10.)/L-BOL]similar to -7). The X-ray lines appear surprisingly narrow and unshifted, reminiscent of those of beta Cru and tau Sco. Their relative intensities confirm the anomalous abundances detected in the optical domain (C strongly depleted, N strongly enriched, O slightly depleted). In addition, the X-ray data favor a slight depletion in neon and iron, but they are less conclusive for the magnesium abundance, which may be solar-like. While no significant changes occur during the XMM-Newton observation, variability in the X-ray domain is detected in the long-term range. The formation radius of the X-ray emission is loosely constrained to < 5 R-circle dot, which allows for a range of models (wind-shock, corona, magnetic confinement,...) though not all of them can be reconciled with the softness of the spectrum and the narrowness of the lines. [less ▲]Detailed reference viewed: 18 (1 ULg) High-resolution X-ray structures of human apoferritin H-chain mutants correlated with their activity and metal-binding sites.Toussaint, Louise in Journal of Molecular Biology (2007), 365(2), 440-52Ferritins are a family of proteins distributed widely in nature. In bacterial, plant, and animal cells, ferritin appears to serve as a soluble, bioavailable, and non-toxic form of iron provider. Ferritins ... [more ▼]Ferritins are a family of proteins distributed widely in nature. In bacterial, plant, and animal cells, ferritin appears to serve as a soluble, bioavailable, and non-toxic form of iron provider. Ferritins from animal sources are heteropolymers composed of two types of subunit, H and L, which differ mainly by the presence (H) or absence (L) of active ferroxidase centres. We report the crystallographic structures of four human H apoferritin variants at a resolution of up to 1.5 Angstrom. Crystal derivatives using Zn(II) as redox-stable alternative for Fe(II), allows us to characterize the different metal-binding sites. The ferroxidase centre, which is composed of sites A and B, binds metal with a preference for the A site. In addition, distinct Zn(II)-binding sites were found in the 3-fold axes, 4-fold axes and on the cavity surface near the ferroxidase centre. To study the importance of the distance of the two metal atoms in the ferroxidase centre, single and double replacement of glutamate 27 (site A) and glutamate 107 (site B), the two axial ligands, by aspartate residues have been carried out. The consequences for metal binding and the correlation with Fe(II) oxidation rates are discussed. [less ▲]Detailed reference viewed: 1 (0 ULg) High-Resolution, Human-Bovine Comparative Mapping Based on a Closed Yac Contig Spanning the Bovine Mh LocusPirottin, Dimitri ; Poncelet, D.; Grobet, Luc et alin Mammalian Genome : Official Journal of the International Mammalian Genome Society (1999), 10(3), 289-93A closed YAC contig spanning the mh locus was assembled by STS content mapping with seven microsatellite markers, eight genes or EST, and nine STS corresponding to YAC ends. The contig comprises 27 YACs ... [more ▼]A closed YAC contig spanning the mh locus was assembled by STS content mapping with seven microsatellite markers, eight genes or EST, and nine STS corresponding to YAC ends. The contig comprises 27 YACs, has an average depth of 4.3 YACs, and spans an estimated 1.2 Mb. A linkage map was constructed based on five of the microsatellite markers anchored to the contig and shown to span 7 cM, yielding a ratio of 160 kb/1 cM for the corresponding chromosome region. Comparative mapping data indicate that the constructed contig spans an evolutionary breakpoint connecting two chromosome segments that are syntenic but not adjacent in the human. Consolidation of human gene order by means of whole genome radiation hybrids and its comparison with the bovine order as inferred from the contig confirm conservation of gene order within segments. [less ▲]Detailed reference viewed: 30 (6 ULg) High-risk human papillomavirus infection of the genital tract of women with a previous history or current high-grade vulvar intraepithelial neoplasiaGoffin, Frédéric ; Mayrand, Marie-Hélène; Gauthier, Philippe et alin Journal of Medical Virology (2006), 78(6), 814-819Human papillomavirus (HPV) infection is associated with high-grade vulvar intraepithelial neoplasia (VIN-3). The prevalence of anogenital HPV infection in women with previously treated VIN-3 has not been ... [more ▼]Human papillomavirus (HPV) infection is associated with high-grade vulvar intraepithelial neoplasia (VIN-3). The prevalence of anogenital HPV infection in women with previously treated VIN-3 has not been documented yet. This cross-sectional study compared high-risk HPV DNA detection rates in women with past (n = 30) and current (n = 22) VIN-3 to those without current or past VIN (n = 86). HPV DNA was detected in vulvar and cervical samples with Hybrid Capture 2 (HC-2). Smoking was associated in multivariate analysis with current VIN-3 (odds ratio (OR) 8.3, 95% confidence interval (CI) 2.0-8.2) and any VIN-3 history (OR 6.5, 95% CI 2.5-16.5). High-risk HPV DNA was found on the vulva of 64%, 33%, and 20% of women with current VIN-3, past VIN-3, and without previous or current VIN, respectively. After controlling for age and smoking, high-risk HPV vulvar infection was associated with cervical high-risk HPV infection (OR 8.6, 95% CI 2.8-26.5; P = 0.001). After controlling for age, HPV infection was more often multifocal in women with current VIN-3 compared to women with previous but no current VIN-3 lesion (OR 17.6, 95% CI 1.4-227.2). Multifocal vulvar HPV infection was detected in women with previous or active VIN-3. Longitudinal studies are required to determine if the multifocality of HPV infection on the vulva could explain the high recurrence rate of VIN-3. [less ▲]Detailed reference viewed: 1 (0 ULg) High-sensitivity staining of proteins for one- and two-dimensional gel electrophoresis using post migration covalent staining with a ruthenium fluorophore.Tokarski, Caroline; Cren-Olive, Cecile; Fillet, Marianne et alin Electrophoresis (2006), 27(7), 1407-16This paper describes the use of a ruthenium complex ((bis(2,2'-bipyridine)-4'-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophosphate), abbreviated below as ASCQ_Ru) commercially ... [more ▼]This paper describes the use of a ruthenium complex ((bis(2,2'-bipyridine)-4'-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophosphate), abbreviated below as ASCQ_Ru) commercially available and chemically pure. This new ruthenium complex ASCQ_Ru brings an activated ester, allowing the selective acylation of amino acid side chain amines for the post migration staining of proteins separated in 1-DE and 2-DE. The protocol used is a simple three-step protocol fixing the proteins in the gel, staining and then washing, as no lengthy destaining step is required. First the critical staining step was optimized. Although in solution the best described pH for acylating proteins with this reagent is phosphate buffer at pH 7.0, we found that best medium for in-gel staining is unbuffered ACN/water solution (20/80 v/v). The two other steps are less critical and classical conditions are satisfactory: fixing with 7% acetic acid/10% ethanol solution and washing four times for 10 min with water. Sensitivity tests were performed using 1-DE on protein molecular weight markers. We obtained a higher sensitivity than SYPRO Ruby with a detection limit of 80 pg of protein per well. However, contrary to SYPRO Ruby, ASCQ_Ru exhibits a logarithmic dependency on the amount of protein. The dynamic range is similar to SYPRO Ruby and is estimated between three and four orders of magnitude. Finally, the efficiency of the post migration ASCQ_Ru staining for 2-D gel separation is demonstrated on the whole protein extract from human colon carcinoma cells lines HCT 116. ASCQ_Ru gave the highest number of spot detected compared to other common stains Colloidal CBB, SYPRO Ruby and Deep Purple. [less ▲]Detailed reference viewed: 29 (0 ULg)