L'implantation placentaire humaine

in Bulletin de l'Académie Nationale de Médecine (1992), 176(3), 303-7308-9

Like malignant tumor cells, cells of the trophoblast, of the blastocyst and of the hemochorial placenta are highly invasive. This invasiveness is essential for implantation and penetration of the ... [more ▼]

Like malignant tumor cells, cells of the trophoblast, of the blastocyst and of the hemochorial placenta are highly invasive. This invasiveness is essential for implantation and penetration of the epithelial and connective tissues of the endometrium. According to Hertig's data and the observations (henceforth classical) of the Carnegie Foundation, it is commonly admitted that trophoblastic cells penetrate rapidly the blood vessels of the maternal endometrium, open them and allow the establishment of a communication between maternal circulation and fetal tissues from the 20th day after fertilization. As suggested in the classical textbooks of Embryology, the hemochorial placentation would be established very early in human pregnancy. These observations (essentially histological) are actually contradicted by clinical observations performed by Doppler contact echography and by dynamic observations with a microvideo camera implanted in the intervillous placental chamber. Echographic observations coupled with the study of Doppler signals suggest the absence of placental maternal vascularization during the first trimester of pregnancy. It is possible to mount an optical system between the membranes and the uterine wall up to the placental tissue to observe the placental circulation. This direct observation concludes to the absence of maternal vascularization in the intervillous chamber during the first trimester of pregnancy. At the beginning of the 13th week of gestation, uterine arteries open directly into the intervillous chamber ensuring the direct perfusion of placental villi. These informations allow to suggest that the placenta becomes hemochorial at the beginning of the second trimester of pregnancy. [less ▲]

Les implantations industrielles nouvelles de la province de Liège

in Revue de la Société d'Etudes et d'Expansion (1975), (265), 369-385

Based on a survey of all establishments employing 10 workers who were identified between 1948 and 1970 in the Province of Liege, study of new trends in industrial locations

Implementacion de un acuerdo social de rio : contrato de rio en la cuenca hidrographica para la gestion integrada de los recursos hidricos

in Atlas ecotematico de la cuenca del rio Tolomosa (Bolivia) (2011)

Implementation and Preliminary Evaluation of an ISP-Driven Informed Path Selection

in ACM CoNEXT conference (2007, December)

Implémentation d'une méthode de détection du virus de la diarrhée virale bovine au sein de la fondation de la promotion des productions andines Proinpa (Bolivie)

Master's dissertation (2010)

An animal infected by the Bovine Viral Diarrhea Virus will have diarrheas, which can lead to milk production reduction, reproduction problems (and particularly abortion problems) and general weakness ... [more ▼]

An animal infected by the Bovine Viral Diarrhea Virus will have diarrheas, which can lead to milk production reduction, reproduction problems (and particularly abortion problems) and general weakness. This all leads to an economic loss. This is why the virus detection and the carriers, or Persistently Infected (PI) animals, eradication are important. If the virus presence was previously suspected in Bolivia, since 2009 it is certain that there is BVDV infection and the prevalence is high in comparison with others areas. Furthermore, this virus can also infect « New Worlds camelids » which are the alpacas, lamas, vicunas and guanacos species living in South-America. The aim of this study was to implement a BVDV detection method for the Foundation of Andeans Productions Promotion PROINPA in Bolivia. This objective was first met by a prevalence evaluation of the virus using ELISA anti-antibodies analysis. Then, a screening of the tested herds containing animals with a positive serology was done by real-time PCR and finally the PI animals were detected by individual real-time PCR analysis or by two ELISA anti-antigens analysis’s in a 3 week's interval. There was no problem from an infrastructure viewpoint; the laboratories have all the necessary material and equipment and the labor was qualified for both analyses. But the logistic part was more difficult, because the molecular kit has to be preserved at minus twenty degrees. And this kit took 10 days to arrive from Belgium. Because none of the molecular analysis’s succeeded, we concluded that the kit had been damaged during the travel and had been degraded. The kit conservation conditions seemed to be a problem and that is why the elaboration of a new lyophilized kit was tried in Belgium. The lyophilisation of the BVDV sequences, the IPC and EPC was easy but wasn’t possible for the Master MIX (containing the Taq Polymerase (Taq)) and the Reverse Transcriptase (RT). The « Illustra Ready-To-GoTM RT-PCR Beads », from GE Healthcare, are beads made of Taq and RT that can be stored at room temperature. The combination of a lyophilized part of the LSI kit (BVDV sequences, EPC and IPC) with those beads will lead to a molecular BVDV detection kit that can be stored at room temperature. However, this combination doesn’t seem to work. This can be due to a lot of different things like the incompatibility between the primers size and design and the Taq, the temperature activities of the Taq and the RT, the salt concentration, etc. In conclusion, different experiments are required to finalize a qPCR kit resistant to long trip conditions. [less ▲]

Implémentation d’une prise en charge cognitivo-comportementale auprès d’adolescents délinquants dans une unité d’accueil court

in Baileau, Francis (Ed.) Evolution ou rupture ? La justice des mineurs en question (2008)

Implémentation de la fonction de transfert dans un logiciel de simulation thermique pour le bâtiment

E-print/Working paper (2006)

Implementation of a Design Space Approach for Enantiomeric Separations in Polar Organic Solvent Chromatography

in Journal of Pharmaceutical & Biomedical Analysis (2013), 74

This paper focuses on implementing a Design Space approach and on the critical process parameters (CPPs) to consider when applying the Quality by Design (QbD) concepts outlined in ICH Q8(R2), Q9 and Q10 ... [more ▼]

This paper focuses on implementing a Design Space approach and on the critical process parameters (CPPs) to consider when applying the Quality by Design (QbD) concepts outlined in ICH Q8(R2), Q9 and Q10 to analytical method development and optimization for three chiral compounds developed as modulators of small conductance calcium-activated potassium (SK) channels. In this sense, an HPLC method using a polysaccharide-based stationary phase containing a cellulose tris (4-chloro-3-methylphenylcarbamate) chiral selector in polar organic solvent chromatography mode was considered. The effects of trifluoroacetic acid (TFA) and n-hexane concentration in an acetonitrile (MeCN) mobile phase were investigated under a wide range of column temperatures. Good correlations were found between the observed data obtained after using a central composite design and the expected chromatographic behaviours predicted by applying the design of experiments-design space (DoE-DS) methodology. The critical quality attribute represented here by the separation criterion (Scrit) allowed assessing the quality of the enantioseparation. Baseline separation for the compounds of interest in an analysis time of less than 20 minutes was possible due to the original and powerful tools applied which facilitated an enhanced method comprehension. Finally, the advantage of the DoE-DS approach resides in granting the possibility to concurrently assess robustness and identify the optimal conditions which are compound dependent. [less ▲]

Implementation of a RTW program for low back pain workers in Belgium : Assessment of the workplace intervention component

in WDPI 2010, Book of abstracts (2010)

Implementation of a www site presenting Liège viewed from space using satellilte images

in Earth Observation by Satellite (2002)