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See detailFate of premalignant clones during the asymptomatic phase preceding lymphoid malignancy.
Moules, Vincent; Pomier, Carole; Sibon, David et al

in Cancer Research (2005), 65(4), 1234-43

Almost all cancers are preceded by a prolonged period of clinical latency during which a combination of cellular events helps move carcinogen-exposed cells towards a malignant phenotype. Hitherto ... [more ▼]

Almost all cancers are preceded by a prolonged period of clinical latency during which a combination of cellular events helps move carcinogen-exposed cells towards a malignant phenotype. Hitherto, investigating the fate of premalignant cells in vivo remained strongly hampered by the fact that these cells are usually indistinguishable from their normal counterparts. Here, for the first time, we have designed a strategy able to reconstitute the replicative history of the bona fide premalignant clone in an animal model, the sheep experimentally infected with the lymphotropic bovine leukemia virus. We have shown that premalignant clones are early and clearly distinguished from other virus-exposed cells on the basis of their degree of clonal expansion and genetic instability. Detectable as early as 0.5 month after the beginning of virus exposure, premalignant cells displayed a two-step pattern of extensive clonal expansion together with a mutation load approximately 6 times higher than that of other virus-exposed cells that remained untransformed during the life span of investigated animals. There was no fixation of somatic mutations over time, suggesting that they regularly lead to cellular death, partly contributing to maintain a normal lymphocyte count during the prolonged premalignant stage. This equilibrium was finally broken after a period of 18.5 to 60 months of clinical latency, when a dramatic decrease in the genetic instability of premalignant cells coincided with a rapid increase in lymphocyte count and lymphoma onset. [less ▲]

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See detailThe fate of the adjacent motion segments after lumbar fusion.
Gillet, Philippe ULg

in Journal of Spinal Disorders & Techniques (2003), 16(4), 338-45

Lumbar spine fusion is a commonly performed procedure in various pathologic conditions of the spine. Its role remains debated, and moreover, delayed complications may occur, among which is transitional ... [more ▼]

Lumbar spine fusion is a commonly performed procedure in various pathologic conditions of the spine. Its role remains debated, and moreover, delayed complications may occur, among which is transitional segment alteration leading to recurrence of back pain, gross instability, and neurologic symptoms. Little is known about the long-term prevalence of this complication because of a lack of specific studies. We analyzed the fate of the transitional segments in a homogeneous group of patients operated on during a 14-year period for degenerative conditions of the lumbar spine resistant to conservative treatment. Follow-up ranged from 2 to 15 years. Seventy-five percent of our study group had a minimal 5-year follow-up. In this subgroup, 41% of the patients developed transitional segment alterations, and 20% needed a secondary operation for extension of the fusion. Potential risk factors such as postoperative delay, length of fusion, and spine imbalance were recognized. The frequency of delayed alterations of the adjacent segment and the severity of symptoms related to this complication in this study raise questions about the justification of fusion procedures in degenerative conditions of the spine without threatening instability. Data from the literature confirm the severity of the problem, but many uncertainties remain because of the lack of homogeneous and complete data on both the normal evolution of motion segments of the lumbar spine with age and the fate of the same segments when transformed in transitional segments. Future prospective studies on the subject are needed and must deal with homogeneous groups of patients. More reconstructive surgical procedures need to be developed to lessen the need for fusion procedures. [less ▲]

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See detailThe fate of the Blal repressor during the induction of the Bacillus licheniformis BlaP beta-lactamase
Filée, Patrice ULg; Benlafya, Kamal; Delmarcelle, Michaël ULg et al

in Molecular Microbiology (2002), 44(3), 685-694

The induction of the Staphylococcus aureus BlaZ and Bacillus licheniformis 749/l BIaP beta-lactamases by beta-lactam antibiotics occurs according to similar processes. In both bacteria, the products of ... [more ▼]

The induction of the Staphylococcus aureus BlaZ and Bacillus licheniformis 749/l BIaP beta-lactamases by beta-lactam antibiotics occurs according to similar processes. In both bacteria, the products of the blal and blaR1 genes share a high degree of sequence homology and act as repressors and penicillin-sensory transducers respectively. It has been shown in S. aureus that the Blal repressor, which controls the expression of BlaZ negatively, is degraded after the addition of the inducer. In the present study, we followed the fate of Blal during beta-lactamase induction in B. licheniformis 749/l and in a recombinant Bacillus subtilis 168 strain harbouring the pDML995 plasmid, which carries the B. licheniformis blaP, blal and blaR1 genes. In contrast to the situation in B. licheniformis 749/l, beta-lactamase induction in B. subtilis 168/pDML995 was not correlated with the proteolysis of Blal. To exclude molecular variations undetectable by SDS-PAGE, two-dimensional gel electrophoresis was performed with cellular extracts from uninduced or induced B. subtilis 168/pDML995 cells. No variation in the Blal isoelectric point was observed in induced cells, whereas the DNA-binding property was lost. Cross-linking experiments with dithiobis(succimidylpropionate) confirmed that, in uninduced recombinant B. subtilis cells, Blat was present as a homodimer and that this situation was not altered in induced conditions. This latter result is incompatible with a mechanism of inactivation of Blal by proteolysis and suggests that the inactivation of Blal results from a non-covalent modification by a co-activator and that the subsequent proteolysis of Blal might be a secondary phenomenon. In addition to the presence of this co-activator, our results show that the presence of penicillin stress is also required for full induction of beta-lactamase biosynthesis. [less ▲]

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See detailFate of the nucleolar vacuole during resumption of cell cycle in pea cotyledonary buds
Jennane, A.; Thiry, Marc ULg; Diouri, M. et al

in Protoplasma (2000), 210(3-4), 172-178

Meristematic cells of pea cotyledonary buds blocked in G(0-1) state contain a small nucleolus with a large central clear area surrounded by a fibrillar rim. The nucleolar structure varies according to the ... [more ▼]

Meristematic cells of pea cotyledonary buds blocked in G(0-1) state contain a small nucleolus with a large central clear area surrounded by a fibrillar rim. The nucleolar structure varies according to the cell cycle from the G(0-1)-blocked state until the first mitoses occurring between 24 and 27 h after removal of the main stem. In order to better identify and understand the role of the central area in the nucleolar function, its content was investigated by cytochemical and terminal deoxynucleotidyl transferase-immunogold methods. The central area showed the characteristics of a vacuole commonly constituted of the condensed chromatin, ribonucleoprotein granules, and lack of argyrophilic proteins. 3 h alter decapitation, a thickening of the fibrillar rim occurred, accompanied by an increase of granules in the vacuole. After 6 h, the unique vacuole broke up into two to four small vacuoles in which the granules are more abundant. After 12 h the nucleolus acquired compact structure with few minute vacuoles dispersed over the fibrillar component. During the whole cell cycle, the condensed chromatin is always observed in the vacuole. Our findings suggest that the appearance of the vacuoles is subsequent to the output of preribosomes from nucleolus. These vacuoles might play a role in condensation and decondensation of the chromatin. [less ▲]

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See detailFate of thiazolidine ring during fragmentation of penicillin by exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61
Frère, Jean-Marie ULg; Ghuysen, Jean-Marie ULg; Vanderhaeghe, Hubert et al

in Nature (1976), 260(5550), 451-454

LIKE various beta-lactamases, acylases and esterases1, the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61 degrades benzylpenicillin and other beta-lactam antibiotics2-4. The R61 enzyme ... [more ▼]

LIKE various beta-lactamases, acylases and esterases1, the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61 degrades benzylpenicillin and other beta-lactam antibiotics2-4. The R61 enzyme, however, markedly differs from the other penicillin-degrading enzymes in causing fragmentation of the penicillin nucleus. By using 8-14C-benzylpenicillin (benzyl labelled) as substrate, one of the fragments produced was shown to be 14C-phenylacetylglycine5. The reaction with the R61 enzyme is also peculiar in that it is a slow process. This is because of the long half life of the stoichiometnc complex transitorily formed between the antibiotic and the enzyme. Thus, for example, the value of the half life for the complex formed with benzylpenicillin is 80 min in 10 mM phosphate buffer (pH 7.0) and at 37 °C. As breakdown of the complex proceeds, however, phenylacetylglycine (when benzylpenicillin is used as substrate) is released and the enzyme concomitantly recovers its ability to bind penicillin. We have now characterised the fragment (hereby designated as the Y product) arising from the thiazolidine ring of penicillin as a result of the fragmentation of the antibiotic molecule by the R61 enzyme. [less ▲]

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See detailFate of unstimulated Latissimus Dorsi transposed into the chest and applied to cardiomyoplasty
Radermecker, M. A.; Reznik, M.; Vivario, M. et al

in Research in Surgery (1994), 6(1), 35-39

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See detailFate of zinc in a sandy Luvisol submitted to 100 years of massive irrigation with raw waste waters: a multi-scale approach
Dere, Christelle ULg; Lamy, Isabelle; Cornu, Sophie

Conference (2007, July)

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See detailFatigue index reproducibility in isokinetic testing
Paulus, Julien ULg; Croisier, Jean-Louis ULg; Bosquet, Laurent et al

in De Haan, Arnold; De Ruiter, Jo; Tsolakidis, Elias (Eds.) Book of abstracts (2014, July)

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See detailLa fatigue motrice et ses implications dans les pathologies du système nerveux
PHAN BA, Remy ULg; MARTIN, Didier ULg

Scientific conference (2013, March 13)

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See detailFatigue musculaire
Julia, Marc; Perrey, S.; Dupeyron, A. et al

Book published by Masson (2010)

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See detailFatigue, facteur de risque de lésions musculaires chez le sportif?
Croisier, Jean-Louis ULg; Forthomme, Bénédicte ULg; Maquet, Didier ULg et al

in Julia, M.; Perrey, S.; Dupeyron, A. (Eds.) et al Fatigue musculaire (2010)

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See detailFatistes des villes, fatistes des champs
Henrard, Nadine ULg

in Leroux, Xavier (Ed.) Vers une poétique du discours dramatique au Moyen Âge. Actes du Colloque international organisé au Palais Neptune de Toulon les 13 et 14 novembre 2008 (2011)

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See detailFattening of Belgian Blue double muscled culled females : metabolism data
Cabaraux, Jean-François ULg; de Behr, Vanessa; Hornick, Jean-Luc ULg et al

in Book of Abstracts of the 52nd Annual Meeting of the European Association for Animal Production (2001)

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See detailFattening of Belgian Blue double muscled culled females : technical data
Cabaraux, Jean-François ULg; de Behr, Vanessa; Hornick, Jean-Luc ULg et al

in Book of abstracts of the 52nd Annual Meeting of the European Association for Animal Production (2001)

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See detailFattening of Belgian Blue double-muscled culled females: animal performance, carcass characteristics and meat quality
Cabaraux, Jean-François ULg; Hornick, Jean-Luc ULg; Dufrasne, Isabelle ULg et al

in Annales de Médecine Vétérinaire (2003), 147(6, DEC-JAN), 423-431

In order to measure Animal performance and, carcass and meat characteristics of Belgian Blue double-muscled culled females, a fattening trial was carried out on a total of 78 animals. Fifteen females were ... [more ▼]

In order to measure Animal performance and, carcass and meat characteristics of Belgian Blue double-muscled culled females, a fattening trial was carried out on a total of 78 animals. Fifteen females were slaughtered at the beginning of the trial and 63 were fattened on a maize silage based diet. The initial average live weight was 571.2kg. The total fattening gain was 91.1 kg for a period of 91.4 days. At slaughter, the warm carcass weight was 405.9kg and the killing-out proportion 63.3%. The carcasses were characterised by a high proportion of muscles (67.6%) and rather low proportions of adipose tissue (18.2%) and bones (14.2%). The muscle and adipose tissue accretions were 36.0 and 28.2 kg. Meat was lean as indicated by high protein content (88.5%) and low fat content (6.6%) in dry matter. A high proportion of polyunsaturated fatty acids was also found (6.8 mol %). [less ▲]

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See detailFattening of young bulls: comparaison of three breeds and two managements
Cabaraux, Jean-François ULg; Hornick, Jean-Luc ULg; Istasse, Louis ULg et al

in Book of absracts of the 54th Annual Meeting of the European Association for Animal Production (2003)

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See detailFattori di infertilità e di infecondità nella vacca
Hanzen, Christian ULg

Learning material (2011)

Le thème général proposé a fait l'objet de 5 présentations orales.

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See detailFatty Acid Binding Protein [Fragment] from Ovis aries placenta - Access number P83204
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2002)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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See detailFatty acid composition of residual yolk and liver from broiler chicks hatching from omega-3 enriched eggs
Koppenol, Astrid; Delezie, Evelyne; Wang, Yufeng et al

in Proceedings of the XXIV World's Poultry Congress (2012)

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