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See detailEffects of Nimesulide and Sodium Diclofenac on Interleukin-6, Interleukin-8, Proteoglycans and Prostaglandin E2 Production by Human Articular Chondrocytes in Vitro
Henrotin, Yves ULg; Labasse, A. H.; Simonis, P. E. et al

in Clinical and Experimental Rheumatology (1999), 17(2), 151-60

OBJECTIVES: The aim of this study was to investigate the effects of two nonsteroidal anti-inflammatory drugs (NSAIDs), nimesulide and sodium diclofenac, on the production of proteoglycans (PG ... [more ▼]

OBJECTIVES: The aim of this study was to investigate the effects of two nonsteroidal anti-inflammatory drugs (NSAIDs), nimesulide and sodium diclofenac, on the production of proteoglycans (PG), prostaglandin E2 (PGE2) and cytokines (IL-6 and IL-8) by human articular chondrocytes in vitro. METHODS: Enzymatically isolated chondrocytes were cultured under constant agitation in a well defined culture medium. Specific radioimmunoassays were used to quantify PG and PGE2 production. Cytokine production (IL-6 and IL-8) was assayed by enzyme amplified sensitivity immunoassays (EASIAs). RESULTS: At a concentration of 3 micrograms/ml, nimesulide did not affect the PG production by chondrocytes. This concentration was superior to the highest level of nimesulide found in the synovial fluid of patients with rheumatoid arthritis 3 hours after the last oral administration of nimesulide (100 mg twice daily for 7 days). At 6 micrograms/ml a significant reduction in the PG content was obtained in the cellular phase in 5 out of the 8 cultures investigated. No similar effect was observed in the culture supernatants. Above this concentration nimesulide inhibited PG production in a dose-dependent manner. At concentrations ranging from 0.005 to 1 microgram/ml diclofenac did not significantly alter PG production. At therapeutic concentrations PGE2 production was totally inhibited by nimesulide, thus suggesting that PG inhibition is not linked to PGE2 production. Nimesulide inhibited PGE2 production by unstimulated (IC50 = 6 ng/ml) and IL-1 beta-stimulated (IC50 = 6.9 ng/ml) chondrocytes. At these concentrations, PGE2 production was fully inhibited by diclofenac. Furthermore, both nimesulide and diclofenac at therapeutic concentrations significantly decreased spontaneous and IL-1 beta-stimulated IL-6 production by human chondrocytes, but did not modify IL-8 production. CONCLUSION: From the results of this study we conclude that nimesulide and diclofenac at therapeutic concentrations are potent inhibitors of PGE2 and IL-6 production while they do not modify proteoglycan or IL-8 production. [less ▲]

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See detailEffects of nitrate and ammonium on larvae of Rana temporaria from the Pyrenees.
Oromi Farrús, Neus ULg; Sanuy, D.; Vilches, M.

in Bulletin of Environmental Contamination & Toxicology (2009), 82

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See detailEffects of nitrogen fertilizer on sward heigh, animal performance and economic balance in pasture grazed by growing fattening bulls
Dufrasne, Isabelle ULg; Raskin, Pascale; Mayombo, Asangule Pierre et al

in Abstracts of the 47th meeting of the European Association for Animal Production (1996)

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See detailEffects of nitroglycerin on the nociception specific blink reflex
Di Clemente, Laura; Magis, Delphine ULg; Coppola, Gianluca et al

in Cephalalgia : An International Journal of Headache (2005, October), 25(10), 888

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See detailEffects of Nitrous Oxide on Spectral Entropy of the Eeg During Surgery under Balanced Anaesthesia with Sufentanil and Sevoflurane
Hans, Pol ULg; Dewandre, Pierre-Yves; Brichant, Jean-François ULg et al

in Acta Anaesthesiologica Belgica (2005), 56(1), 37-43

BACKGROUND: Spectral entropy of the electroencephalogram (EEG) has been proposed to monitor anaesthetic depth. We investigated the effect of nitrous oxide on response (RE) and state entropy (SE) of the ... [more ▼]

BACKGROUND: Spectral entropy of the electroencephalogram (EEG) has been proposed to monitor anaesthetic depth. We investigated the effect of nitrous oxide on response (RE) and state entropy (SE) of the EEG during lumbar disc surgery under anaesthesia with sufentanil and sevoflurane. METHODS: In an open study, anaesthesia was induced with propofol and sufentanil, and maintained with 2% end-tidal sevoflurane concentration in air/oxygen (FiO2 = 0.4) in 25 patients. During surgery, nitrous oxide was randomly administered either at 0 or at 60% end-tidal concentration in 10 (control group) and 15 patients (nitrous oxide group), respectively. RE and SE were recorded at 2.5 min intervals for 10 min before randomization and for 25 min either continuously (control) or after achieving the target nitrous oxide concentration. RESULTS: Two patients who received nitrous oxide were excluded from statistical analysis because of protocol violation. Nitrous oxide provoked a significant decrease in RE and SE from 46.2 +/- 11.1 and 44.3 +/- 11.1 to a lowest value of 27.8 +/- 8.3 and 27.1 +/- 8.9, respectively. The decrease in entropy persisted during the 25 min recording period. CONCLUSIONS: Addition of nitrous oxide during balanced anaesthesia with sufentanil and sevoflurane provokes a decrease in response and state entropy of the EEG during lumbar disc surgery. [less ▲]

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See detailEffects of Non Genetic and Crossbreeding Factors on Daily Milk Yield of Jersey x Sahiwal x Ankole Cows in Burundi
Hatungumukama, G.; Hornick, Jean-Luc ULg; Detilleux, Julien ULg

in Journal of Animal and Veterinary Advances (2009), 8(4), 794-798

The study was conducted at the Rukoko station located at 21 km North-Western of Bujumbura in the Imbo natural region, in the South of Rusizi River. The objective was to assess the effects of year of ... [more ▼]

The study was conducted at the Rukoko station located at 21 km North-Western of Bujumbura in the Imbo natural region, in the South of Rusizi River. The objective was to assess the effects of year of lactation, genetic group, lactation length, season and parity on the Daily Milk Yield (DMY) of Jersey (J) x Sahiwal (S) x Ankole (A) crossed cows. Milk yields were obtained from cows that have been both Suckled and Milked (SM) or only Milked (M). Data were analyzed using the procedure GLM in SAS on SM and M cows, separately. All factors affected significantly DMY (p<0.001). A significant increase was observed from 1989-1991 due to the improvement of management techniques. During this period, the production raised from 10.07 +/- 0.22 L-11.50 +/- 0.17 L day(-1) for SM cows. Unfortunately, the 1993 civilian war destabilized the managerial techniques and consequently, the milk production decreased. Highest milk yields were observed in crossed cows with 50% of Jersey and 50% of Sahiwal inheritances. Peak yields occurred at the 43rd (12.67 +/- 0.42 L) and 56th (6.42 +/- 0.45 L) days in milk for SM and M cows, respectively. Milk production was significantly higher in January (11.04 +/- 0.23 L day(-1)), in the middle of the rainy season, than in July (7.02 +/- 0.18 L day(-1)), in the middle of the dry season. Milk yield in SM cows increased from 8.15 +/- 0.17 L-9.46 +/- 0.16 L day(-1) during the 1st and the 2nd lactation, respectively. [less ▲]

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See detailEffects of non-genetic and crossbreeding factors on daily milk yield of Ayrshire x (Sahiwal x Ankole) cows in Mahwa station (Burundi)
Hatungumukama, G.; Sidikou, D. L.; Leroy, Pascal ULg et al

in Livestock Science (2007), 110(1-2), 111-117

The study was carried out at the Mahwa station located in Bututsi natural region in South of Burundi at 1850 m of altitude. The objective of the study was to assess the effects of days in milk, cow's ... [more ▼]

The study was carried out at the Mahwa station located in Bututsi natural region in South of Burundi at 1850 m of altitude. The objective of the study was to assess the effects of days in milk, cow's calving age, type of crossbreeding, year and month of lactation, parity on daily milk yield of crossbreds Ayrshire (AY) x Sahiwal (S) x Ankole (A) cows. Milk yields were obtained from 9 cows (S) that have been both milked and suckled during the entire lactation, on 8 cows (M) only milked during the entire lactation and on 150 cows (SM) suckled before and milked after weaning. Data were analyzed using the procedure GLM in SAS for S,M, and SM cows separately. All factors affected significantly daily milk yields (P < 0.001). Least-squares means (LSM) for daily milk yields were highest at the 8th (7.16 +/- 0.07 1), 8th (7.63 +/- 0.35 1), and 7th (7.34 +/- 0.39 1) day in milk for SM, S and M lactations, respectively. The Wood model was able to detect 2 main groups of curve shape: standard for SM and S cows and atypical decreasing for M cows. An important decrease in milk production was observed around the 120th day in SM cows corresponding to the weaning period. Crossbreeding improved milk production and highest yields were observed with 50% to 75% of Ayrshire inheritance (LSM = 5 +/- 0.05 to 5.51 +/- 0.02 1/day). A significant increase was observed from 1977 to 1992 due to the improvement of genetic and management techniques. During this period, LSM raised from 1.26 +/- 0.11 1 to 4.74 +/- 0.03 1 per day for SM cows. Unfortunately, the 1993 civilian war destabilized the managerial techniques and consequently, the milk production decreased in Mahwa station, In all cows, milk production was significantly higher from December to May (LSM - 3.96 +/- 0.03 1/day) during the rainy season than in September (LSM = 3.12 +/- 0.03 1/day) at the end of the dry season. (C) 2006 Elsevier B.V. All rights reserved. [less ▲]

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See detailEffects of Non-Genetic Factors on Daily Milk Yield of Friesian Cows in Mahwa Station (South Burundi)
Hatungumukama, G.; Leroy, P. L.; Detilleux, Johann ULg

in Revue d'Elévage et de Médecine Vétérinaire des Pays Tropicaux (2008), 61(1), 45-49

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See detailEffects of nucleophiles on the breakdown of the benzylpenicilloyl-enzyme complex EI formed between benzylpenicillin and the exocellular DD-carboxypeptidase--transpeptiase of Streptomyces strain R61
Marquet, Alberto; Frère, Jean-Marie ULg; Ghuysen, Jean-Marie ULg et al

in Biochemical Journal (1979), 177(3), 909-916

Serine is one of the enzyme residues with which benzylpenicillin collides as a result of its binding to the Streptomyces strain-R61 DD-carboxypeptidase-transpeptidase enzyme. Nucleophilic attack occurs on ... [more ▼]

Serine is one of the enzyme residues with which benzylpenicillin collides as a result of its binding to the Streptomyces strain-R61 DD-carboxypeptidase-transpeptidase enzyme. Nucleophilic attack occurs on C(7) of the bound antibiotic molecule with formation of a benzylpenicilloyl-serine ester linkage, i.e. formation of the benzylpenicilloyl-enzyme EI complex. To reject the bound penicilloyl moiety and consequently to recover its initial activities, the strain-R61 enzyme has developed two possible mechanisms. Pathway A is a direct attack of the serine ester linkage by an exogenous nucleophile, resulting in the transfer of the benzylpenicilloyl moiety to this nucleophile. In pathway B, the benzylpenicilloyl moiety is first fragmented by C(5)-C(6) cleavage and the enzyme-bound phenylacetylglycyl residue thus produced is in turn transferred to the nucleophile. Pathway B occurs with water, glycylglycine and other amino compounds. Both pathways A and B occur with glycerol, other ROH nucleophiles and neutral hydroxylamine. The nucleophilic attacks are enzyme-catalysed. [less ▲]

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See detailEffects of nutritional programming on growth and metabolism caused by albumen removal in an avian model
Willems, E.; Koppenol, A.; De Ketelaere, B. et al

in Journal of Endocrinology (2015), 225

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See detailEffects of O2 tension and glucose concentration on the cellular respiration of equine articular chondrocytes in culture.
Schneider, Nicole ULg; Lejeune, Jean-Philippe ULg; Mouithys-Mickalad, Ange ULg et al

Poster (2003)

In vivo, articular chondrocytes are exposed to 5 to 10% O21,2. Chondrocytes are also suspected to produce reactive oxygen species when submitted to anoxia/re-oxygenation3,4. We were interested to study ... [more ▼]

In vivo, articular chondrocytes are exposed to 5 to 10% O21,2. Chondrocytes are also suspected to produce reactive oxygen species when submitted to anoxia/re-oxygenation3,4. We were interested to study the effects of O2 and glucose variations on cellular respiration, glucose consumption and lactate production. Equine articular chondrocytes were cultured in suspension for 2 days under 5 or 21 % O2 in the gaseous phase, and with 0, 1.0 or 4.5 g/L glucose. The viable cells were then counted and the respiration rate (O2 consumption) of 10.106 cells was monitored by oxymetry for 2 hours; after oxymetry, glucose and lactate were measured in the medium (enzymatic assays). After 2 days, the cell viability was the best at 5% O2 and 1g/L glucose; it decreased at 4.5 g/L glucose and was the worst at 0g/L glucose, for the two O2 tensions (n=3). There was no obvious difference of the respiration rate between cells cultured at 5 and 21% O2, but respiration of chondrocytes was surprisingly low. When cells were submitted to 20 min anoxia at 0% O2, the O2 consumption was doubled at re-oxygenation for cells previously cultured at 21% O2. Glucose and lactate values found in the medium after oxymetry : lactate release in medium was similar (36.23 and 34.57 mg/L respectively) for cells cultured with 1g glucose and 5 or 21% O2 conditions; lactate values were low (2.03 and 8,63 mg/L respectively) for 4.5 g glucose and 5 or 21% O2. Glucose uptake was not different whatever the culture conditions. These results indicate a low cellular respiration with a lactate production linked to the glucose concentration in the medium, and raise the question of the capacity of chondrocytes to produce ROS in vivo starting from the mitochondrial chain. [less ▲]

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See detailEFFECTS OF O2 TENSION AND GLUCOSE CONCENTRATION ON THE CELLULAR RESPIRATION OF EQUINE ARTICULAR CHONDROCYTES IN CULTURE.
Schneider, Nicole ULg; Lejeune, Jean-Philippe ULg; Duyckaerts, Claire ULg et al

Poster (2003)

In vivo, articular chondrocytes are exposed to 5 to 10% O2. Chondrocytes are also suspected to produce reactive oxygen species when submitted to anoxia/re-oxygenation. We were interested to study the ... [more ▼]

In vivo, articular chondrocytes are exposed to 5 to 10% O2. Chondrocytes are also suspected to produce reactive oxygen species when submitted to anoxia/re-oxygenation. We were interested to study the effects of O2 and glucose variations on cellular respiration, glucose consumption and lactate production. Equine articular chondrocytes were cultured in suspension for 2 days under 5 or 21 % O2 in the gaseous phase, and with 0, 1.0 or 4.5 g/L glucose. The viable cells were then counted and the respiration rate (O2 consumption) of 10.106 cells was monitored by oxymetry for 2 hours; after oxymetry, glucose and lactate were measured in the medium (enzymatic assays). After 2 days, the cell viability was the best at 5% O2 and 1g/L glucose; it decreased at 4.5 g/L glucose and was the worst at 0g/L glucose, for the two O2 tensions (n=3). There was no obvious difference of the respiration rate between cells cultured at 5 and 21% O2, but respiration of chondrocytes was surprisingly low. When cells were submitted to 20 min anoxia at 0% O2, the O2 consumption was doubled at re-oxygenation for cells previously cultured at 21% O2. Glucose and lactate values found in the medium after oxymetry: lactate release in medium was similar (36.23 and 34.57 mg/L respectively) for cells cultured with 1g glucose and 5 or 21% O2 conditions; lactate values were low (2.03 and 8,63 mg/L respectively) for 4.5 g glucose and 5 or 21% O2. Glucose uptake was not different whatever the culture conditions. These results indicate a low cellular respiration with a lactate production linked to the glucose concentrationin the medium, and raise the question of the capacity of chondrocytes to produce ROS in vivo starting from the mitochondrial chain. [less ▲]

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See detailEffects of obesity on lung function and airway reactivity in healthy dogs
Manens, Jefferson ULg; Bolognin, M; Bernaerts, Frederique ULg et al

in Veterinary Journal (2012), 193(1), 217-221

The present study investigated the effects of bodyweight (BW) gain on respiratory function and airway responsiveness in healthy Beagles using barometric whole body plethysmography (BWBP). Six adult dogs ... [more ▼]

The present study investigated the effects of bodyweight (BW) gain on respiratory function and airway responsiveness in healthy Beagles using barometric whole body plethysmography (BWBP). Six adult dogs were examined before and after a fattening diet. The high-energy diet induced a mean increase in BW of 41 ± 6%. BWBP basal parameters were recorded prior to airway reactivity testing (using increasing concentrations of histamine nebulisations). An airway responsiveness index (H-Penh300) was calculated as the histamine concentration necessary to reach 300% of basal enhanced pause (Penh, bronchoconstriction index). The same dogs underwent a doxapram hydrochloride (Dxp) stimulation testing 2 weeks later. Basal measurements showed that obese dogs had tidal volume per kg (TV/BW) that was significantly decreased whilst respiratory rate (RR) increased significantly. H-Penh300 decreased significantly in obese Beagles, indicating increased bronchoreactivity. Dxp administration induced a significant increase in TV/BW, minute volume per kg (MV/BW), peak inspiratory and expiratory flows per kg (PIF/BW and PEF/BW) in both normal and obese dogs although the TV/BW increase was significantly less marked in the obese group. In conclusion, obesity induced changes in basal respiratory parameters, increased bronchoreactivity and a blunted response to Dxp-induced respiratory stimulation. This combination of basal respiratory parameters, bronchoreactivity testing and pharmacological stimulation testing using non-invasive BWBP can help characterize pulmonary function and airway responsiveness in obese dogs. [less ▲]

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See detailEffects of omeprazole on the canine cerebrospinal fluid composition
Girod, Maud ULg; Allerton, Fergus; Gommeren, Kris ULg et al

in Proceedings of the 23rd ECVIM-CA CONGRESS (2013, September)

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See detailEffects of oral contraception with ethinylestradiol and drospirenone on oxidative stress in women 18-35 years old.
De Groote, D.; PERRIER d'HAUTERIVE, Sophie ULg; Pintiaux, Axelle ULg et al

in Contraception (2009), 80(2), 187-93

Background Oral contraceptives (OCs) with estrogens and progestins may affect oxidative stress (OS) status. Study design A group of 32 women using oral contraceptives (OCU) containing 0.03 mg ... [more ▼]

Background Oral contraceptives (OCs) with estrogens and progestins may affect oxidative stress (OS) status. Study design A group of 32 women using oral contraceptives (OCU) containing 0.03 mg ethinylestradiol and 3 mg drospirenone have been compared to a matched control group of 30 noncontraception users (NCU). Blood levels of antioxidants, trace elements and markers of lipid peroxidation were assessed by biochemical methods. A microarray analysis of whole blood mRNA levels of 200 genes involved in OS-dependant pathway was also performed. Results Levels of zinc, vitamin E and antibodies to oxidized low-density lipoproteins (LDLs) were not significantly different between the two groups. On the other hand, significant increases in the mean levels of lipid peroxides (+176%, p<.001), oxidized LDLs (+145%, p<.002), copper (+103%, p<.001), Cu/Zn ratio (+100%, p<.001) and a significant decrease in the mean level of β-carotene (−41%, p<.01) were observed in the OCU compared to NCU. There was a highly significant positive correlation between the lipid peroxide levels and the copper-to-zinc ratio. From the 200 genes tested by microarray, one coding for HSP70 was significantly up-regulated (log2 fold change=+ 0.45, p<.02) and one coding for inducible nitric oxide synthase significantly down-regulated (log2 fold change=−0.24, p<.05) in the OCU compared to the NCU. Conclusions The recently introduced combination of ethinylestradiol and drospirenone induced the heightening of lipid peroxidation correlated with high levels of copper, a situation that could be associated with increased cardiovascular risk. [less ▲]

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See detailEffects of oral preoperative carbohydrate on early postoperative outcome after thyroidectomy
Lauwick, Séverine ULg; Kaba, Abdourahmane ULg; Maweja, Sylvie ULg et al

in Acta Anaesthesiologica Belgica (2009), 60(2), 67-73

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See detailEffects Of Oral Supplementation With Bovine Colostrum On The Immune System Of Weaned Piglets
Boudry, Christelle ULg; Buldgen, André; Portetelle, Daniel ULg et al

in Research In Veterinary Science (2007), 83(1), 91-101

The aim of this study was to evaluate the influence of bovine colostrum supplementation on the immune system of weaned piglets in a context of a full ban on in-feed antibiotics. After weaning at 21 days ... [more ▼]

The aim of this study was to evaluate the influence of bovine colostrum supplementation on the immune system of weaned piglets in a context of a full ban on in-feed antibiotics. After weaning at 21 days, 24 outbred piglets were fed with a diet supplemented daily for three weeks with 0, 1 or 5 g of colostrum. Feed intake, growth performance, haematological parameters, and serum and local anti-colostrum immunoglobulin levels were examined. Lymphocytes from the blood, spleen and gut-associated lymphoid tissues were analysed for phenotype as well as for their ability to produce cytokines. The stimulation index (SI) of mononuclear cells from different organs was obtained after colostral or mitogenic stimulation. Feed intake, growth and haematological parameters were not significantly affected by colostrum. Total serum IgA levels were increased after colostrum supplementation, with a transient decrease in total IgG. Local anti-colostrum immunization was observed in colostrum-fed piglets. The CD21+/CD3+ cells populations of the ileal Peyer’s patch (iPP) were markedly affected. The SI of lymphocyte populations changed significantly whereas, naive blood lymphocytes were not stimulated in vitro in the presence of bovine colostrum, suggesting local anti-colostrum immunization and an absence of direct mitogenic effects of the colostrum. Both Th1 and Th2 cytokine production was present in the different organs of colostrum-fed piglets. Bovine colostrum especially stimulated iPP cells. [less ▲]

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See detailEffects of oral supplementation with bovine colostrum on the immunity of weaned piglets
Boudry, Christelle ULg; Buldgen, André; Dehoux, Jean-Paul

in Proccedings of the 19th International Piv Veterinary Society Congress (2006, July)

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