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Peer Reviewed
See detailEvaluation of a new immunoenzymetric assay for CK-MB (tandem-E CKMB)
Chapelle, Jean-Paul ULg; El Allaf, M.; Heusghem, C.

in Annales de Biologie Clinique (1985), 43

Detailed reference viewed: 4 (0 ULg)
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See detailEvaluation of a new laboratory high-shear mixer
Evrard, Brigitte ULg; Peerboom, Claude; Delattre, Luc ULg

Conference (1993)

Detailed reference viewed: 16 (0 ULg)
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See detailEvaluation of a new rapid test for the detection of norovirus antigen in comparison with Real Time RT-PCR
HUYNEN, Pascale ULg; Mauroy, Axel ULg; Gérard, Catherine ULg et al

Poster (2013, September)

Diagnosis of NoV infection mainly relies on molecular methods. A detection of viral antigens can also be performed by immunochromatographic assays, and may be useful in outbreak settings. The aim of this ... [more ▼]

Diagnosis of NoV infection mainly relies on molecular methods. A detection of viral antigens can also be performed by immunochromatographic assays, and may be useful in outbreak settings. The aim of this study was to compare the performances of the new RDT ImmunoCardSTAT!®Norovirus (Meridian Bioscience®, Europe) with a real time RT-PCR. [less ▲]

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See detailEvaluation of a novel anion-exchange restricted-access sorbent for on-line sample clean-up prior to the determination of acidic compounds in plasma by liquid chromatography
Rbeida, O.; Christiaens, B.; Hubert, Philippe ULg et al

in Journal of Chromatography. A (2004), 1030(1-2), 95-102

A new kind of silica-based restricted-access material (RAM) with anionic properties has been tested in pre-columns for on-line solid-phase extraction of acidic compounds from directly injected plasma ... [more ▼]

A new kind of silica-based restricted-access material (RAM) with anionic properties has been tested in pre-columns for on-line solid-phase extraction of acidic compounds from directly injected plasma samples prior to their determination by reversed-phase liquid chromatography (LC), using the column-switching technique. The outer surface of the porous RAM particles contains hydrophilic diol groups while diethylaminoethyl (DEAE) groups are bound to the internal surface which gives the sorbent the properties of a weak anion exchanger towards low-molecular-mass compounds. Due to an appropriate pore diameter (about 6 nm), macromolecules, such as proteins, are physically excluded from the pores and flushed directly out during the sample clean-up process, while small compounds have access to the inner surface and can be retained mainly by electrostatic interactions. The retention capability of this novel packing material has been tested for some hydrophilic acidic compounds such as aspartic acid, glutamic acid, ascorbic acid and acetylcysteine as well as for some more hydrophobic drugs such as naproxen, ibuprofen and diclofenac, used as model compounds. The influence of the composition of the washing liquid on the retention of the analytes in the pre-column has been investigated. The efficiency of the sorbent to clean-up complex matrices was also tested using human plasma and urine samples. A generic washing liquid composition was then selected in order to obtain efficient and selective sample clean-up as well as a high recovery of the acidic analytes. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 23 (5 ULg)
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See detailEvaluation of a portable equine metabolic measurement system
Duvivier, V. H.; Van Erck, Emmanuelle; De Moffarts, Brieuc et al

in 7th International Conference on Equine Exercise Physiology (2006)

Detailed reference viewed: 44 (10 ULg)
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See detailEvaluation of a protocol for fast localised abdominal sonography in horses (FLASH) admitted for colic.
Busoni, Valeria ULg; De Busscher, Virginie; Lopez, Diego et al

in Veterinary Journal (2011)

The aim of this prospective study was to establish a protocol for fast localised abdominal sonography of horses (FLASH) admitted for colic. The FLASH protocol was then presented to clinicians without ... [more ▼]

The aim of this prospective study was to establish a protocol for fast localised abdominal sonography of horses (FLASH) admitted for colic. The FLASH protocol was then presented to clinicians without extensive ultrasound (US) experience to determine whether they could learn to use it in less than 15 min. The clinical subjects comprised 36 horses that had been referred for colic over a 2 month period. Each horse was examined at admission and FLASH findings at seven topographical locations were compared to serial clinical examinations, surgical and non-surgical outcomes, or with post-mortem reports. FLASH was able to show free abdominal fluid and abnormal intestinal loops, with a mean time of 10.7 min required to complete the protocol. The positive and negative predictive values of requirement for surgery of dilated turgid small intestinal loops using FLASH were 88.89% and 81.48%, respectively. The results suggested that FLASH is a technique that can be used in an emergency setting by veterinarians without extensive US experience to detect major intra-abdominal abnormalities in horses with colic. [less ▲]

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See detailEVALUATION OF A RAPID BEDSIDE SCORING SYSTEM FOR MICROCIRCULATION VIDEOS ACQUIRED FROM DOGS
Gommeren, Kris ULg; Allerton, Fergus; Morin, Elise et al

in Journal of Veterinary Emergency and Critical Care (in press)

Detailed reference viewed: 9 (0 ULg)
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See detailEvaluation of a rapid turbidimetric myoglobin immunoassay
Lammers, M.; Dati, F.; Kapmeyer, W. H. et al

in Clinical Chemistry (1991), 37

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See detailEvaluation of a sanitarian public-private partnership in Benin. An ethnographic study case.
Schnitzler, Marie ULg

Conference (2013, June 28)

While private clinics are generally recognized as important care givers in current African cities, the sector remains under-studied in the social sciences. The research for my master thesis focused on the ... [more ▼]

While private clinics are generally recognized as important care givers in current African cities, the sector remains under-studied in the social sciences. The research for my master thesis focused on the creation of a hospital anchored to a public-private partnership, including the World Bank, the Beninese Health Ministry and a private non-governmental association. The main purpose of my enquiry is to understand how the status of this hospital influences (or not) its organization and its use by clients. Therefore, I firstly analyze the emergence and the diversity of the Beninese private health sector as well as the connotations of the notion of public-private partnership. These two points help to understand the particular context of creation of the studied public-private partnership and how it was imposed to the Ministry by the World Bank. Secondly, my research shows how the creation is nowadays read in different ways by the various actors in order to claim their rights on the management of the hospital. Thirdly, focusing on collaboration between the different categories of workers and their relationships with clients, I describe formal and informal organization of this private hospital. This part underlines the particularities of private direction in comparison with well-known studies of public centers. Using these data, I also try to understand why clients come to this specific hospital. I argue that status is not as important in their choice. [less ▲]

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See detailEvaluation of a screening program for postmenopausal osteoporosis in primary care setting in Belgium
Goemare, S.; Kaufman, Jean-Marc; De Bacquer, D. et al

in Osteoporosis International (2002, November), 13(Suppl.3), 39

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See detailEvaluation of a set of E. coli reporter strains as physiological tracer for estimating bioreactor hydrodynamic efficiency
Delvigne, Frank ULg; Ingels, Sophie; Thonart, Philippe ULg

in Process Biochemistry (2010), 45(11), 1769-1778

A set of different green fluorescent protein (GFP) Escherichia coli reporter strains have been evaluated in mini- and stirred bioreactors operating in fed-batch mode with different degrees of ... [more ▼]

A set of different green fluorescent protein (GFP) Escherichia coli reporter strains have been evaluated in mini- and stirred bioreactors operating in fed-batch mode with different degrees of perturbations in order to estimate their potential use as process-related stress biosensor. The mini-bioreactor platform comprises a set of parallel shake flasks operating in fed-batch mode. The advantage of this system is its high experimental throughput for the evaluation of the GFP synthesis capacity of our reporter strains. In the case of classical shake flask system, no significant evolution of GFP synthesis have been observed, considering the reduced microbial growth period allowed by the system, whereas in the case of fed-batch operated mini-bioreactors, evolution of GFP synthesis, as well as GFP distribution among the microbial population, has been observed for three preselected strains (prpoS, puspA and posmC::gfp). More interestingly, a binary mode of expression has been observed in the case of the cultures carried out with the reporter strains for which GFP synthesis is under the control of the rpoS promoter which is induced under carbon limitation conditions. However, the generation of controlled glucose perturbations is relatively limited in this system and, in a second step fully automated bioreactor with a sclae-down strategy has been used to correlate the response of a prpoS::gfp strains with extracellular glucose perturbations. In the case of the culture performed in perturbed bioreactor (glucose intermittent feeding or glucose addition at the level of the recycle loop of a two-compartment scale-down bioreactor), the slowdown of the GFP synthesis resulting in the observation of a binary repartition of GFP content among the microbial population, has been observed. This observation led to the conclusion that the prpoS::gfp can be used as a biosensor for the validation of a fed-batch profile in industrial-scale bioreactors. [less ▲]

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See detailEvaluation of a weight-adjusted single-bolus plasminogen activator in patients with myocardial infarction: a double-blind, randomized angiographic trial of lanoteplase versus alteplase.
den Heijer, P.; Vermeer, F.; Ambrosioni, E. et al

in Circulation (1998), 98(20), 2117-25

BACKGROUND: Lanoteplase (nPA) is a rationally designed variant of tissue plasminogen activator with greater fibrinolytic potency and slower plasma clearance than alteplase. METHODS AND RESULTS: InTIME ... [more ▼]

BACKGROUND: Lanoteplase (nPA) is a rationally designed variant of tissue plasminogen activator with greater fibrinolytic potency and slower plasma clearance than alteplase. METHODS AND RESULTS: InTIME (Intravenous nPA for Treatment of Infarcting Myocardium Early), a multicenter, double-blind, randomized, double-placebo angiographic trial, evaluated the dose-response relationship and safety of single-bolus, weight-adjusted lanoteplase. Patients (n=602) presenting within 6 hours of acute myocardial infarction were randomized and treated with either a single-bolus injection of lanoteplase (15, 30, 60, or 120 kU/kg) or accelerated alteplase. The primary objective was to determine TIMI grade flow at 60 minutes. Angiographic assessments were also performed at 90 minutes and on days 3 to 5. Follow-up was continued for 30 days. Lanoteplase achieved its primary objective, demonstrating a dose-response in TIMI grade 3 flow at 60 minutes (23.6% to 47.1% of subjects, P<0. 001). Similar results were observed at 90 minutes (26.1% to 57.1%, P<0.001). At 90 minutes, coronary patency (TIMI 2 or 3) increased across the dose range up to 83% of subjects at 120 kU/kg lanoteplase compared with 71.4% with alteplase. Thus, at this dose, lanoteplase was superior to alteplase in restoring coronary patency (difference, 12%; 95% CI, 1% to 23%). The early safety experience in this study suggests that lanoteplase was well tolerated at all doses with safety comparable to that of alteplase. CONCLUSIONS: Lanoteplase, a single-bolus, weight-adjusted agent, increased coronary patency at 60 and 90 minutes in a dose-dependent fashion. Coronary patency at 90 minutes was achieved more frequently with 120 kU/kg lanoteplase than alteplase. In this study, safety with lanoteplase and alteplase was comparable. InTIME-II, a worldwide mortality trial, will evaluate efficacy and safety with this promising new agent. [less ▲]

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See detailEvaluation of Accuracy of Pulse Oximetry in Newborn Calves
Uystepruyst, Christophe; Coghe, Joost; Bureau, Fabrice ULg et al

in Veterinary Journal (2000), 159(1), 71-76

In human medicine, pulse oximetry is widely used to measure non-invasively and accurately the percentage of oxygen saturation of arterial haemoglobin (SpO(2)). Recently, pulse oximetry has been used in ... [more ▼]

In human medicine, pulse oximetry is widely used to measure non-invasively and accurately the percentage of oxygen saturation of arterial haemoglobin (SpO(2)). Recently, pulse oximetry has been used in calves, but its accuracy has not been evaluated in newborn calves. The purpose of this study was to evaluate the accuracy of a pulse oximeter in newborn calves by comparing SpO(2) with arterial oxyhaemoglobin saturation (SaO(2)) obtained by use of a blood gas analyser. Fifty-five newborn calves were investigated from birth to 20 days old. Pulse oximetry readings and arterial blood samples were performed 5, 15, 30, 45, 60 min, 2, 3, 6, 12, 24 h and 1 and 3 weeks after birth. The transmission-type sensors of the pulse oximeter were fixed at the recommended site in the bovine species (at the base of the calf tail, where the skin had been shaved and was not pigmented) and arterial blood samples were withdrawn from the subclavian artery and analysed for SaO(2). Five-hundred paired data of SaO(2) and mean SpO(2)(mSpO(2)) were collected. Linear regression of the pooled data indicated a highly significant correlation of mSpO(2) with SaO(2) (r = 0.87;P< 0.001; mSpO(2) = 15.8 + 0.84 SaO(2)). The overall data bias value was positive (+2.1%), which indicated that the pulse oximeter tended to overestimate the SaO(2). The bias value for each SaO(2) category tended to become higher for lower ranges of SaO(2). Precision was also lower when SaO(2) values were low. The lower the SaO(2) value, the higher the positive bias (overestimation) and the lower the precision. These results suggest that pulse oximetry provides a relatively accurate non-invasive, immediate and portable method to monitor SaO(2) and to evaluate objectively the pulmonary function effectiveness in newborn calves during their adaptation to extra-uterine life. [less ▲]

Detailed reference viewed: 50 (2 ULg)