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See detailEstrogen activation revisited: control of local metabolism in the brain
Charlier, Thierry ULg

Scientific conference (2012)

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See detailEstrogen and pain: a study in aromatase knock-out mice using the formalin model.
Multon, Sylvie ULg

Conference (2004, September)

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See detailEstrogen Receptor-Beta in Quail: Cloning, Tissue Expression and Neuroanatomical Distribution
Foidart, Agnès ULg; Lakaye, Bernard ULg; Grisar, Thierry ULg et al

in Journal of Neurobiology (1999), 40(3), 327-42

A partial estrogen receptor-beta (ERbeta) cDNA had been previously cloned and sequenced in Japanese quail. The 3'- and 5'-rapid amplification of cDNA ends techniques were used here to identify a cDNA ... [more ▼]

A partial estrogen receptor-beta (ERbeta) cDNA had been previously cloned and sequenced in Japanese quail. The 3'- and 5'-rapid amplification of cDNA ends techniques were used here to identify a cDNA sequence of the quail ERbeta that contains a complete open reading frame. For the first time in an avian species, this cDNA sequence and the corresponding amino acid sequence are described. They are compared with the known ERbeta sequences previously described in mammals and with the ERalpha sequences identified in a selection of mammalian and avian species. The analysis by Northern blotting of the ERbeta mRNA expression in the brain and kidneys revealed the presence of several transcripts. The presence of ERbeta identified by reverse transcriptase-polymerase chain reaction demonstrated a widespread distribution quite different from the distribution of ERalpha. The complete neuroanatomical distribution of ERbeta mRNA as determined by in situ hybridization with 35S- and 33P-labeled oligoprobes is also presented. Transcripts are present in many nuclei implicated in the control of reproduction such as the medial preoptic nucleus, the nucleus striae terminalis, and the nucleus taeniae, the avian homologue of the amygdala. These data demonstrate the presence of ERbeta in a nonmammalian species and indicate that the (neuro)-anatomical distribution of this receptor type has been conserved in these two classes of vertebrates. The role of this receptor in the control of reproduction and other physiological processes should now be investigated. [less ▲]

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See detailEstrogen replacement therapy and inhibition of bone resorption
Reginster, Jean-Yves ULg; Sarlet, N; Albert, Adelin ULg et al

in Revue du Rhumatisme et des Maladies Osteo-Articulaires (1992), 59

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See detailEstrogen-deficient female but not male aromatase knockout (ArKO) mice exhibit "depressive-like" symptoms
Bakker, Julie ULg; Dalla, C.; Antoniou, K. et al

in Hormones & Behavior (2004, June), 46(1), 127

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See detailEstrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast
Didimo Imazaki, Pedro Henrique ULg; Brose, François ULg; Jauniaux, Thierry ULg et al

in BioMed Research International (2015), 2015

Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using ... [more ▼]

Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using a luciferase reporter gene assay (RGA). None of the PCB 138, 153, or 180 or their mixture induced a response in the RGA. o,p'-DDT was the most potent xenoestrogen fromthe DDT group, inducing a response already at 80 ng/mL. From the HCH and HCB group, only 𝛽-HCH (at 400 and 2000 ng/mL) and 𝛿-HCH (at 2000 ng/mL) displayed estrogenic activities.These 13 organochlorines were determined by GC-MS in 12 samples of North Sea harbor porpoise blubber. The PCBs were the main contaminants. Within each group, PCB 153 (6.0 × 102∼4.2 × 104 𝜇g/kg), p,p'- DDE (5.1 × 102∼8.6 × 103 𝜇g/kg), and HCB (7.6 × 101∼1.5 × 103 𝜇g/kg) were the compounds found in highest concentrations.The hormonal activity of the porpoise blubber samples was also assayed in RGA, where two samples showed estrogenic activity, seven samples showed antiestrogenic activity, and one sample showed both estrogenic and antiestrogenic activity. Our results suggest that the 13 POPs measured by GC-MS in the samples cannot explain alone the estrogenicity of the extracts. [less ▲]

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See detailEstrogens and pain : a study in aromatase knock-out mice using the formalin model
Multon, Sylvie ULg; Mosen, Jeanine; Wouters, Murielle ULg et al

Poster (2004, July)

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See detailEstrogens and tumor microenvironment
Pequeux, Christel ULg

Conference (2013)

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See detailEstrogens control female sexual motivation and receptivity in quail.
de Bournonville, Catherine ULg; Ball, Gregory, F.; Balthazart, Jacques ULg et al

Poster (2013, November 10)

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See detailEstrogens reduce the expression of YKL-40 in the retina: Implications for eye and joint diseases
Rakic, Jean-Marie ULg; Lambert, Vincent ULg; Deprez, Manuel ULg et al

in Investigative Ophthalmology & Visual Science (2003), 44(4), 1740-1746

PURPOSE. To identify modifications in the gene expression profile of the ocular posterior segment in ovariectomized (OVX) mice with and without substitutive estradiol therapy and to select differentially ... [more ▼]

PURPOSE. To identify modifications in the gene expression profile of the ocular posterior segment in ovariectomized (OVX) mice with and without substitutive estradiol therapy and to select differentially expressed genes that could be relevant to the natural history of human age-related macular degeneration (AMD). METHODS. Chorioretinal tissues from two groups of 25 treated and untreated OVX mice were analyzed by using cDNA array technology. The expression level of selected genes was confirmed in triplicate by RT-PCR and related to the estrogenic status of the animals. Expression of the YKL-40 gene was further investigated in intact or diseased human retinas and in a murine model of experimental choroidal neovascularization (CNV), using laser pressure catapulting. RESULTS. Of the approximately, 10,000 genes screened, only YKL-40 expression was significantly downregulated by 17-beta-estradiol. YKL-40 was expressed in intact human neural retina and in the RPE. The expression of YKL-40 was upregulated in experimental CNV and in neovascular membranes extracted from patients affected by the exudative form of AMD. CONCLUSIONS. These observations indicate that YKL-40 expression in the retina is modulated by serum levels of estradiol. This protein could be relevant to the development of AMD and is also a new mediator to take into account when evaluating the broad consequences of hormonal replacement therapy. [less ▲]

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See detailEstructura y regulation de los genes de la familia de la prolactina
Martial, Joseph ULg

in Archivos de Biologia y Medicina Experimentales (1981), (14), 21

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See detailEstrus synchronization, artificial insemination, and pregnancy diagnosis in water buffaloes (Bubalus bubalis)
Hanh, NV; Huu, QX; Uoc, NT et al

in Reproduction, Fertility and Development (2007), 19(1), 198

One of the big obstacles in management of reproductive activity in water buffaloes is their silent estrus. This characteristic prevents the determination of time for artificial insemination (AI ... [more ▼]

One of the big obstacles in management of reproductive activity in water buffaloes is their silent estrus. This characteristic prevents the determination of time for artificial insemination (AI), resulting in lengthening of the calving interval (Nguyen et al. 1990 Proc. FAO Workshop, Bulgaria, 210–216). Pregnancy-associated glycoprotein (PAG) measurement in maternal blood is an effective method of early pregnancy diagnosis in ruminant species (Zoli et al. 1992 Biol. Reprod. 46, 83–92). The present study describes a preliminary attempt to improve buffalo reproductive performance by association of three different approaches: synchronization of estrus by hormonal treatment, followed by two AI, and finally pregnancy diagnosis based on PAG measurements. A total of 33 buffalo females (4 to 15 years old), with no apparent estrus during a period of 9 to 36 months after last calving, were used. Each female received 2 injections of 5 mL of PGF2α (Intervet, France) 10 days apart (Day -12 and Day -2), one injection of 500 IU of PMSG (SABC, Vietnam) at Day -5, and 1000 IU of hCG (Intervet, France) on the day of AI (Day 0). A second AI was performed 8 h after hCG injection. Blood samples (9 mL) were collected from each female at Days 20, 25, 40, and 45 after AI. Blood samples were withdrawn by jugular venipuncture (heparinized Vacutainer tubes) and kept in a cool box until centrifugation (500g, 10 min). Plasma samples were stored at -20°C until assayed for PAG. Pregnancy diagnosis was considered negative, doubtful, or positive according to the PAG concentrations (<0.6 ng mL-1, 0.6 to 0.8 ng mL-1, and >0.8 ng mL-1, respectively). Clinical observation of females after insemination showed that the total rate of animals that exhibited signs of estrus until Day 45 was 9.1% (3/33). These 3 females were not considered for pregnancy diagnosis. Table 1 shows the results of pregnancy diagnosis in 30 water buffalo females. These results show that fertilization is easily obtained after estrus synchronization in buffaloes and that measurement of PAG concentrations in maternal plasma can be used for pregnancy diagnosis from Day 40 after AI. [less ▲]

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