E-VEIL. Recherche menée dans le cadre de l’objectif 2. Rapport de recherche pour le Fonds Social Européen.
Chenu, Florent ; ;
Report (2004)Detailed reference viewed: 6 (0 ULg)
E. ALES et T. NOVITZ (éd.), Collective Action and Fundamental Freedoms in Europe : Striking the Balance, Anvers, Intersentia, Social Europe Series, 2010
in Revue Trimestrielle de Droit Européen (2011), 1Detailed reference viewed: 59 (14 ULg)
E. coli HU dimers dynamics studied by NMR
; Damblon, Christian ;
HU is a 2x90 residues, dimeric bacterial histone-like protein involved in chromosome compaction and other DNA-related processes. E. coli HU proteins are composed of three forms: two homodimers α2 and β2 ... [more ▼]
HU is a 2x90 residues, dimeric bacterial histone-like protein involved in chromosome compaction and other DNA-related processes. E. coli HU proteins are composed of three forms: two homodimers α2 and β2 (α and β sharing 70% of sequence identity) and one heterodimer (αβ). Dimeric forms relative abundance varies during cell growth and in response to environmental changes, suggesting that each dimer plays different physiological roles. Unlike other HU proteins which melt through a single step (N2<=>2D), E. coli dimers melt according to a two-step mechanism (N2<=>I2<=>2D). The native dimer, N2, melts partially into a dimeric intermediate, I2, which in turn yields the unfolded monomers, D. Circular Dichroism studies indicate that the intermediate, I2, corresponds to an HU dimer having partly lost its a-helices. Here we compared dynamic properties and structural features of E. Coli HU dimers at different temperatures in order to determine the secondary strutural elements still present in the I2 intermediate. Isotopically labelled proteins (15N, 13C) have been used to perform backbone assignment for all dimeric forms using sequential triple-resonance experiments. 15N-HSQC at different temperatures (288-323K) were recorded .Intensity variations and chemical shift perturbations allow the identification of the secondary element lost in the intermediate I2 confirming the high dynamics of arms responsible for protein-DNA interactions and the thermal stability of HTH motif in all dimeric forms. [less ▲]Detailed reference viewed: 60 (2 ULg)
E. Du Perron en het ‘menselijk apport’ in de Franse 19e-eeuwse poëzie
in Cahiers voor een lezer (2011)Detailed reference viewed: 13 (1 ULg)
E. Dufour, Les néokantiens. Valeur et vérité, Paris, Vrin, 2003.
in Dialogue : Canadian Philosophical Review = Dialogue : Revue Canadienne de Philosophie (2005), 44(1, Winter), 169-172Detailed reference viewed: 37 (6 ULg)
E. R. Squibb Lectures on Chemistry of Microbial Products. The Bacterial DD-carboxypeptidase Enzyme System: A New Insight into the Mode of Action of Penicillin.
Book published by University of Tokyo Press (1977)Detailed reference viewed: 15 (0 ULg)
E.C. Treaty & European Citizenship. Regional Integration and Cultural Diversity: the case of european citizenship
in Foblets; Dundes Renteln, Alison; Gaudreault-Desbiens, Jean-François (Eds.) Cultural Diversity and the Law. State Responses from (2010)Detailed reference viewed: 17 (0 ULg)
E.coli prpoS::gfp strain as biosensor of glucose heterogeneity inside industrial bioreactors
Brognaux, Alison ; Delvigne, Frank ; Thonart, Philippe
Poster (2010, December 08)
• OBJECTIVE: Escherichia coli is a microorganism widely used in the industry for the production of recombinant proteins. The performances obtained at the laboratory level are not reproducible at a large ... [more ▼]
• OBJECTIVE: Escherichia coli is a microorganism widely used in the industry for the production of recombinant proteins. The performances obtained at the laboratory level are not reproducible at a large scale. Actually, the mixing operation is not efficient enough: gradients of glucose and oxygen appear when operating in fed-batch mode (addition of glucose during the culture). These gradients cause adverse impacts on the production of biomass and recombinant protein. The aim of this work is to use the microbial population as biocaptor of the encoutered stress inside heterogeneous industrial bioreactors to better scale-up and regulate these reactors. • MATERIALS AND METHODS: A plasmid carrying a stress promoter followed by the coding sequence of the Green Fluorescent Protein (GFP) is introduced in the bacterial host (in our case, the strain E. coli K12 will be considered as a model organism). When the cell is submitted to given stress condition, GFP synthesis is induced and accumulated into the cytoplasm, leading to the increase of the cell's fluorescence. Flow cytometry detection is used in order to quantify the fluorescence at the single cell level. Obtained results are frequency histograms of fluorescenceintensity in the microbial population • RESULTS: The rpoS gene is a gene of the general stress response, mainly induced at the entrance to stationary phase (during a lack of glucose). The tracking of the GFP fluorescence linked to the activation / repression of the rpoS promoter gives good results. Indeed, there is appearance of a segregation at the level of the GFP content among the microbial population. The intensity of the segregation, as well as its time of appearance during the culture can be related to the bioreactor mixing efficiency. • CONCLUSION: prpoS::gfp strains can be used as biosensors of the heterogeneity of glucose encountered inside industrial reactors. • POTENTIAL APPLICATIONS & KEY BENEFITS: These strains could be used to validate a fed-batch regulation (addition of glucose) at the industrial level. [less ▲]Detailed reference viewed: 82 (21 ULg)
E.M.W. Tillyard and C.S. Lewis, The Personal Heresy
in Revue des Langues Vivantes = Tijdschrift voor Levende Talen (1967), 33(3), 335-335Detailed reference viewed: 4 (0 ULg)
E1 Transitions of Astrophysical Interest in Neutral Oxygen
; Biémont, Emile ; et al
in Journal of Physics : B Atomic Molecular & Optical Physics (1991), 24Detailed reference viewed: 23 (1 ULg)
EACTS guidelines for the use of patient safety checklists.
; ; et al
in European Journal of Cardio - Thoracic Surgery (2012), 41(5), 993-1004
The Safety Checklist concept has been an integral part of many industries that face high-complexity tasks for many decades and in industries such as aviation and engineering checklists have evolved from ... [more ▼]
The Safety Checklist concept has been an integral part of many industries that face high-complexity tasks for many decades and in industries such as aviation and engineering checklists have evolved from their very inception. Investigations of the causes of surgical deaths around the world have repeatedly pointed to medical errors that could be prevented as an important cause of death and disability. As a result, the World Health Organisation developed and evaluated a three-stage surgical checklist in 2007 demonstrating that complications were significantly reduced, including surgical infection rates and even mortality. Together with the results from other large cohort studies into the utility of the surgical checklist, many countries have fully implemented the use of surgical checklists into routine practice. A key factor in the successful implementation of a surgical checklist is engagement of the staff implementing the checklist. In surgical specialties such as our own it was quickly seen that there were many important omissions in the generic checklist that did not cover issues particular to our specialty, and thus the European Association for Cardio-Thoracic Surgery embarked on a process to create a version of the checklist that might be more appropriate and specific to cardiothoracic surgery, including checks on preparations for excessive bleeding, perfusion arrangements and ICU preparations, for example. The guideline presented here summarizes the evidence for the surgical checklist and also goes through in detail the changes recommended for our specialty. [less ▲]Detailed reference viewed: 18 (3 ULg)
EACVI appropriateness criteria for the use of cardiovascular imaging in heart failure derived from European National Imaging Societies voting.
; ; et al
in European heart journal cardiovascular Imaging (2016)
This paper presents the first European appropriateness criteria for the use of cardiovascular imaging in heart failure, derived from voting of the European National Imaging Societies representatives. The ... [more ▼]
This paper presents the first European appropriateness criteria for the use of cardiovascular imaging in heart failure, derived from voting of the European National Imaging Societies representatives. The paper describes the development process and discusses the results. [less ▲]Detailed reference viewed: 16 (3 ULg)