Genetical metabolomics of flavonoid biosynthesis in Populus: a case study

in Plant Journal (2006), 47(2), 224-237

Genetical metabolomics [metabolite profiling combined with quantitative trait locus (QTL) analysis] has been proposed as a new tool to identify loci that control metabolite abundances. This concept was ... [more ▼]

Genetical metabolomics [metabolite profiling combined with quantitative trait locus (QTL) analysis] has been proposed as a new tool to identify loci that control metabolite abundances. This concept was evaluated in a case study with the model tree Populus. Using HPLC, the peak abundances were analyzed of 15 closely related flavonoids present in apical tissues of two full-sib poplar families, Populus deltoides cv. S9-2 x P. nigra cv. Ghoy and P. deltoides cv. S9-2 x P. trichocarpa cv. V24, and correlation and QTL analysis were used to detect flux control points in flavonoid biosynthesis. Four robust metabolite quantitative trait loci (mQTL), associated with rate-limiting steps in flavonoid biosynthesis, were mapped. Each mQTL was involved in the flux control to one or two flavonoids. Based on the identities of the affected metabolites and the flavonoid pathway structure, a tentative function was assigned to three of these mQTL, and the corresponding candidate genes were mapped. The data indicate that the combination of metabolite profiling with QTL analysis is a valuable tool to identify control points in a complex metabolic pathway of closely related compounds. [less ▲]

Genetically engineered polypeptides as a new tool for inorganic nano-particles separation in water based media

in Journal of Materials Chemistry (2011), 21

The present paper relates a method for the separation of an insoluble inorganic powder out of a mixture of several insoluble powders with different chemical compositions, using genetically engineered ... [more ▼]

The present paper relates a method for the separation of an insoluble inorganic powder out of a mixture of several insoluble powders with different chemical compositions, using genetically engineered inorganic binding peptides (GEPI). GEPI are small peptides that recognize and specifically bind an inorganic solid material. This GEPI is anchored to magnetic beads for easy recovery of the powder of interest from the mixture. [less ▲]

Genetics and genomics of energy balance measured in milk using mid-infrared spectroscopy

Poster (2012, August)

Genetics and Inflammatory Bowel Disease: From Association Studies to Wide Genome Screen

in Acta Gastro-Enterologica Belgica (1997), 60(3, Jul-Sep), 201-3

Genetics and regulation of bacterial virulence: towards the molecular version of Koch's postulates

Conference given outside the academic context (2005)

The genetics in Cushing's syndrome

Scientific conference (2009, December)

Genetics of Cushing's Syndrome

in Neuroendocrinology (2010), 92((supp. 1)), 6-10

Cushing's syndrome (CS) is characterized by pathologically elevated free glucocorticoid levels. Endogenous hypercortisolism is usually due to ACTH-secreting pituitary corticotropic adenomas and less often ... [more ▼]

Cushing's syndrome (CS) is characterized by pathologically elevated free glucocorticoid levels. Endogenous hypercortisolism is usually due to ACTH-secreting pituitary corticotropic adenomas and less often due to ectopic ACTH-secreting neuroendocrine neoplasms or ACTH-independent adrenal cortisol hypersecretion. CS is a serious chronic disease leading to a several-fold increase in cardiovascular morbidity and mortality. Multiple genetic alterations have been described in the setting of sporadic corticotropinoma formation. Changes in the expression profiles have been demonstrated in growth factors and their receptors, cell-cycle regulators and in various genes related to hormonal gene transcription, synthesis and secretion. Sporadic adrenal adenomas and carcinomas may demonstrate dysfunction in genes such as TP53 among others. Cushing's disease can be an inherited condition also. Multiple endocrine neoplasia type 1 (MEN1) and familial isolated pituitary adenomas (FIPA) together account for 5% of pituitary adenomas. Cushing's disease occurs infrequently in an inherited setting in both of these conditions. To date only 2 cases of Cushing's disease have been described in association with mutations in AIP. One case of Cushing's disease has been reported as part of MEN4, a rare MEN1-like syndrome due to mutation in the CDKN1B gene. Carney complex (CNC) due to PRKAR1A mutations in most cases is associated with CS, mainly as a cause of bilateral adrenal hyperplasia. The cAMP signaling pathway is affected in this setting. In recent times the involvement of genes such as PDE11A, PDE8B and others have expanded the spectrum of the genetic pathophysiology of CS. [less ▲]

The Genetics of Growth Hormone Axis

Textual, factual or bibliographical database (2003)

Genetics of lactation persistency

in British Society of Animal Science Occasional Publication (1999), 24

Lactation persistency, often simply called persistency, in general can be defined as the ability to maintain yields during the lactation. Persistency has an impact on feed costs, health, and fertility. Of ... [more ▼]

Lactation persistency, often simply called persistency, in general can be defined as the ability to maintain yields during the lactation. Persistency has an impact on feed costs, health, and fertility. Of these three components affected by persistency, the impact of persistency on health, i.e. metabolic stress of the cow leading to health problems, may be most important nowadays. Numerous suggestions for criteria of persistency exist. Often simple ratios of part-lactation yields, e.g. the ratio of yield in the first and last 100 days of lactation, have been used. New approaches have used results from the application of random regression test day models developed for the genetic evaluation of yield traits. Many studies have unfortunately neglected the effect of gestation on persistency but acknowledged that an improved persistency should lead to an improved reproductive performance. Both relationships should be considered in genetic analyses and recommendations for improvement of management decisions. Today the correct description of persistency plays a prominent role to obtain correct genetic evaluations based on test day yields. But, although apparently trivial, a direct genetic analysis of lactation persistency and even more an inclusion of this trait into selection programmes clearly is a complicated task. A reason for this, amongst others, is that management strategies for feeding during the lactation and handling of the reproductive performance that are most often not recorded, are likely to mask the real persistency. Future studies on the genetics of persistency should also seek a strong interaction of geneticists and physiologists as persistency is fundamentally confounded with the problem of metabolic stress. Today, a recommendation of the inclusion of persistency in selection programmes appears to be premature and more studies, e. g. on the association of persistency with longevity, could aid in this process. [less ▲]

Genetics of migraine: Possible links to neurophysiological abnormalities

in Headache (2002), 42(5), 365-377