Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
See detailEstablishment of a new method for rapid and precise estimation of apple proliferation phytoplasma concentration in periwinkle.
Aldaghi, M.; Massart, Sébastien ULg; Roussel, S. et al

in Communications in agricultural and applied biological sciences (2006), 71(3 Pt A),

Detailed reference viewed: 9 (2 ULg)
See detailEstablishment of a new Tet-On cell line inducible for expression of CIPAR1 gene
Cornet, Anne ULg

Scientific conference (1998, November 21)

In prostate cancer therapy, the components of the cell death could play an important role: it is conceivable that targeted tranfer of such genes into prostatic cells could be useful. In our laboratory, a ... [more ▼]

In prostate cancer therapy, the components of the cell death could play an important role: it is conceivable that targeted tranfer of such genes into prostatic cells could be useful. In our laboratory, a new gene, the CIPAR1 (fro Castration Induced Prostatic Apoptosis Related 1), was identified by Marc Bruyninx. This gene is upregulated when the rat prostate is induced to undergo apoptosis. To learn more about the cellular function of the CIPAR1, we have decided to establish a stable cell line inducible for the expression of CIPAR1 using the "Tet-On expression system" of Clontech. Thanks to this system, we now possess a Tet-On cell line expressing CIPAR1 under Tet control. It has a high expression level of CIPAR1 and this expression can be quantitatively regulated. We hope that this stable cell line will let us elucidate the cellular functions of CIPAR1. [less ▲]

Detailed reference viewed: 16 (0 ULg)
Full Text
Peer Reviewed
See detailEstablishment of a Rabbit Model for Bovine Herpesvirus Type 5 Neurological Acute Infection
Meyer, Gilles; Lemaire, Mylène; Lyaku, J. et al

in Veterinary Microbiology (1996), 51(1-2), 27-40

This study was conducted to evaluate the suitability of the rabbit as a model for bovine, herpesvirus 5 (BHV-5) acute infection. In a preliminary experiment, a total of 24 one-month old New Zealand white ... [more ▼]

This study was conducted to evaluate the suitability of the rabbit as a model for bovine, herpesvirus 5 (BHV-5) acute infection. In a preliminary experiment, a total of 24 one-month old New Zealand white rabbits were inoculated with BHV-5 or bovine herpesvirus 1 (BHV-1) by the intraconjunctival, intracerebral or intranasal routes. BHV-5 or BHV-1 inoculated in the conjunctiva induced virus proliferation in the eye mucosae and the nasal cavity of rabbits without meningo-encephalitis. On the other hand, only BHV-5 infection by intranasal or intracerebral routes produced a fatal meningo-encephalitis. The intranasal route was used in a further experiment for the establishment of a rabbit model for BHV-5 infection. A total of 45 rabbits were inoculated intranasally with BHV-5 or BHV-1. The results showed that intranasal inoculation of BHV-5 strain N569 in rabbits was followed by the development of a lethal meningo-encephalitis for 66% of rabbits while all BHV-1 infected rabbits remained healthy throughout this experiment (28 days). Analysis between the mortalities of rabbits infected with BHV-5 and BHV-1 were highly significant (p < 0.001). The presence of BHV-5 in the central nervous system (CNS) was confirmed by virus isolation (essentially the cerebrum, midbrain and pons) and by immunohistochemical staining of BHV-5 antigen (essentially in the neurons of the cerebrum) only in BHV-5 infected rabbits showing clinical signs of meningo-encephalitis. The findings obtained confirmed the suitability of a rabbit model for the establishment of BHV-5 neurological acute infection and also as a valuable tool for the comparative study of BHV-5 and BHV-1 neuropathogenicity. [less ▲]

Detailed reference viewed: 25 (7 ULg)
Peer Reviewed
See detailEstablishment of a sedimentation monitoring system of irrigation dams in Burkina Faso: The PADI project
Hallot, Eric ULg; Guyon, Francis; de Thysebaert, Didier et al

Conference (2013, August 31)

Detailed reference viewed: 12 (1 ULg)
Full Text
Peer Reviewed
See detailEstablishment of an interactomic map of the Ets factors family: Towards a better understanding of their roles in oncogenic processes
Rambout, Xavier ULg; Twizere, Jean-Claude ULg; Dequiedt, Franck ULg

in Inserm Workshop: Interactomics: at the crossroads of biology and bioinformatics (2010, March)

Ets transcription factors play key roles in several cancers such as leukemia, prostate cancer and Ewing’s sarcoma. They regulate the expression of genes controlling biological processes such as cellular ... [more ▼]

Ets transcription factors play key roles in several cancers such as leukemia, prostate cancer and Ewing’s sarcoma. They regulate the expression of genes controlling biological processes such as cellular proliferation, differentiation, apoptosis, metastasis, and transformation. This family is characterized by a highly conserved DNA-binding domain (ETS domain) and is classified into subfamilies according to sequence homology between the members. Using a high-throughput yeast two-hybrid (Y2H) method, we tested the interaction of the major splicing variants of the 28 human Ets factors and their functional domains of interest against the last available version of the human ORFeome (hORFeome v5.1). This screen has identified more than 200 new partners of Ets proteins. Further validation of these new interactions together with previously described interactions will enable a global evaluation of the regulation, and normal and cancerous roles of Ets factors. [less ▲]

Detailed reference viewed: 15 (2 ULg)
Full Text
See detailEstablishment of an interactomic map of the Ets factors family: Towards a better understanding of their roles in oncogenic processes
Rambout, Xavier ULg; Simonis, Nicolas; Demoitié, Pauline et al

Poster (2011, April 29)

Ets transcription factors have been involved in several cancers such as leukemia, prostate cancer and Ewing’s sarcoma. They regulate the expression of genes controlling important biological processes such ... [more ▼]

Ets transcription factors have been involved in several cancers such as leukemia, prostate cancer and Ewing’s sarcoma. They regulate the expression of genes controlling important biological processes such as cellular proliferation, differentiation, apoptosis, metastasis, and transformation. This family of transcription factors is characterized by its highly conserved DNA-binding domain called the ETS domain and members are classified into subfamilies based on sequence homology criterion. We built a protein-protein interaction (PPI) network of the 27 Ets proteins and of their individual functional domains using a high-throughput yeast-two hybrid (Y2H) screening method. That Y2H network was expanded with confident literature-curated PPIs to obtain a comprehensive Ets interaction network. By considering connectivity between Ets interaction partners, we were able to segregate highly connected clusters of proteins from that network. Analysis of ontologies enrichment of those clusters enabled to confirm well-established roles and regulations of Ets factors, but also to suggest new ones. Biological validation of one precise cluster could be used as a rule of a thumb to globally confirm the bioinformatic analysis of our Ets PPI network and the potential physiological or pathological roles and regulation of Ets factors. [less ▲]

Detailed reference viewed: 34 (10 ULg)
Full Text
Peer Reviewed
See detailEstablishment of endosymbiosis: The case of cnidarians and Symbiodinium
Fransolet, David ULg; Roberty, Stéphane ULg; Plumier, Jean-Christophe ULg

in Journal of Experimental Marine Biology and Ecology (2012), 420–421

The symbiosis between cnidarians and Symbiodinium algae (dinoflagellates) is the keystone responsible for the formation of the huge and important structures that are coral reefs. Today many environmental ... [more ▼]

The symbiosis between cnidarians and Symbiodinium algae (dinoflagellates) is the keystone responsible for the formation of the huge and important structures that are coral reefs. Today many environmental and/or anthropogenic threats compromise this tight relationship and lead to more frequent events of drastic loss of Symbiodinium pigments and eventually of algae themselves from cnidarians, better known as cnidarian bleaching. While the mechanisms underlying the collapse of the algae–coral symbiosis are progressively getting unraveled, the understanding of the mechanisms involved in the de novo infection of bleached cnidarians by Symbiodinium remains elusive. In this review, we describe the various steps needed to establish a stable symbiotic relationship between Symbiodinium and cnidarians. We review the mechanisms implicated in host–symbiont recognition and in symbiosome formation and persistence, with a special emphasis on the role played by lectins and Rab proteins. A better understanding of these molecular mechanisms may contribute to the development of strategies to promote post-bleaching recovery of corals. [less ▲]

Detailed reference viewed: 61 (9 ULg)
Full Text
Peer Reviewed
See detailEstablishment of Latency Associated with Glycoprotein E (Ge) Seroconversion after Bovine Herpesvirus 1 Infection in Calves with High Levels of Passive Antibodies Lacking Ge Antibodies
Schynts, F.; Lemaire, Mylène; Ros, C. et al

in Veterinary Microbiology (2001), 82(3), 211-22

This study was conducted to investigate the glycoprotein E (gE) antibody response raised after inoculation with a low infectious dose of bovine herpesvirus 1 (BHV-1) in six calves possessing high levels ... [more ▼]

This study was conducted to investigate the glycoprotein E (gE) antibody response raised after inoculation with a low infectious dose of bovine herpesvirus 1 (BHV-1) in six calves possessing high levels of passive immunity from cows repeatedly vaccinated with gE deleted marker vaccine. Four out of the six calves developed gE antibodies 3-5 weeks after infection, whereas the two other ones remained seronegative to gE. After 5 months of infection, the six calves were treated with dexamethasone. Virus was only re-excreted by the four calves which previously seroconverted against gE. The two other calves became seronegative against BHV-1, 30-32 weeks after infection. A second dexamethasone treatment performed 11 months after infection failed to demonstrate a latent infection in these two calves. Moreover, the lack of identification of a cell-mediated immune response, after the two dexamethasone treatments, and the failure to detect BHV-1 DNA sequences in trigeminal ganglia strongly suggest that these two calves were not latently infected. In conclusion, the presence of high levels of maternal immunity lacking gE antibodies does not prevent latency after infection with a low titre of BHV-1. Moreover, latency is associated with a serological response to gE. These results confirm that the gE deletion is a good marker to identify young calves latently infected with a field virus. [less ▲]

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detail- Establishment of normal and transformed root cultures of Artemisia annua L. for artemisinin production.
Jaziri, M.; Shimomura, K.; Yoshimatsu, K. et al

in Journal of Plant Physiology (1995), 145(1-2), 175-177

Transformed cultures of Artemisia annua L. (Asteraceae) were established by the co-culture method using leaf segments of A. annua and Agrobacterium rhizogenes NCIB 8196 or MAFF 03-01724. The hairy root ... [more ▼]

Transformed cultures of Artemisia annua L. (Asteraceae) were established by the co-culture method using leaf segments of A. annua and Agrobacterium rhizogenes NCIB 8196 or MAFF 03-01724. The hairy root clones thus obtained grew vigorously on hormone-free medium, showing the typical transformed morphology. The genetic transformation of the root was proved by the opine assay. Normal root and shoot cultures were also established. A highly specific and sensitive enzyme-linked immuno-sorbent assay (ELISA) method was used for the detection and semi-quantitative determination of artemisinin and structurally related compounds in these cultures. The presence of artemisinin was confirmed by gas chromatography coupled to mass spectrometry (GC-MS) analysis. The hairy roots cultured in the dark produced no detectable level of artemisinin as shown by the adventitious shoots cultured under light conditions. The ELISA analysis of the green hairy roots cultured in liquid medium under a 16 h light/day photoperiod showed the existence of compound(s) structurally related to artemisinin, though normal and hairy roots cultured in the dark give no detectable levels of immuno-signal. [less ▲]

Detailed reference viewed: 63 (8 ULg)
Full Text
Peer Reviewed
See detailEstablishment of parathyroid hormone (PTH) reference on 10 different assay kits: impact of the recruitment of the population
CAVALIER, Etienne ULg; SOUBERBIELLE, Jean-Claude

in Osteoporosis International (2012, March), 23(Supplement 2), 360

Detailed reference viewed: 17 (1 ULg)
Full Text
Peer Reviewed
See detailThe establishment of quantifier constructions for size nouns: a diachronic case study of heap(s) and lot(s)
Brems, Lieselotte ULg

in Journal of Historical Pragmatics (2012), 2(13), 202-231

Detailed reference viewed: 18 (1 ULg)
Full Text
Peer Reviewed
See detailEstablishment of stable human fibroblast cell lines constitutively expressing active Rho-GTPases.
Servotte, S.; Zhang, Z.; Lambert, Charles ULg et al

in Protoplasma (2006), 229(2-4), 215-20

Small GTP-binding proteins of the Rho family (RhoA, Cdc42, Rac1) regulate the organisation and the turnover of the cell's cytoskeleton and adhesion structures. A significant function of these cellular ... [more ▼]

Small GTP-binding proteins of the Rho family (RhoA, Cdc42, Rac1) regulate the organisation and the turnover of the cell's cytoskeleton and adhesion structures. A significant function of these cellular structures is to translate and counterbalance forces applied to, or generated by, cells in order to maintain homeostasis and control cell movement. We therefore hypothesised that Rho-GTPases are directly involved in cellular gravity perception and may participate in the alterations induced in microgravity. To define an adequate cellular model allowing to investigate this issue, we have established stable cell lines constitutively expressing active forms of either RhoA, Cdc42, or Rac1. The three cell lines differ by morphology and by their ability to form filopodia, lamellipodia, and bundles of actin stress fibers. Overexpression of the active form of either RhoA, Cdc42, or Rac1 is compatible with cell viability and does not affect cell population doubling time. Thus, our series of mutant cells appear well suited to gain further knowledge on the molecular mechanisms of cellular gravity perception. [less ▲]

Detailed reference viewed: 43 (3 ULg)
See detailEl Estado, el mercado y la diversidad cultural
Martiniello, Marco ULg

in Aubarell, Gemma; Zapata, Ricard (Eds.) Inmigracion y proceso de cambio (2004)

Detailed reference viewed: 42 (0 ULg)
Full Text
Peer Reviewed
See detailEstado, mercado y diversidad cultural
Martiniello, Marco ULg

in Revista de Occidente (Madrid, Spain : 1923) (2003), 268

Detailed reference viewed: 41 (1 ULg)
See detail¿Estamos preparados para la ISO26000?
Parra Santamaria, Lusmiriam Andrea ULg

Conference (2011, March 17)

It is a presentation of what ISO26000 implies for the new international society and its differences with other RSC instruments.

Detailed reference viewed: 17 (1 ULg)
Peer Reviewed
See detailEstandarización de un ensayo de PCR en tiempo real para la detección y cuantificación del Virus de la Leucosis Bovina en sangre y semen de toros seropositivos
Rossich, L.; González, F.; Gutiérrez, G. et al

Poster (2008, September 21)

Detailed reference viewed: 6 (0 ULg)
Full Text
See detailEstatuto personal y sociedad multicultural: el papel de la autonomia de la voluntad
Carlier, Jean-Yves ULg

in Calvo Caravaca, A.I.; Iriarte Angel, J.L. (Eds.) Estatuto personal y multiculturalidad de la familia (2000)

Detailed reference viewed: 21 (0 ULg)