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See detailDevelopment of an HPTLC method for determination of hypoglycin A in aqueous extracts of seedlings and samaras of Acer species.
Habyarimana, Jean Belt Adélite ULiege; Baise, Etienne; Douny, Caroline ULiege et al

E-print/Working paper (2017)

Hypoglycin A (HGA) is a toxin contained in seeds of the sycamore maple tree (Acer pseudoplatanus). Ingestion of this amino acid causes equine atypical myopathy (AM) in Europe. Another variety, A. negundo ... [more ▼]

Hypoglycin A (HGA) is a toxin contained in seeds of the sycamore maple tree (Acer pseudoplatanus). Ingestion of this amino acid causes equine atypical myopathy (AM) in Europe. Another variety, A. negundo, is claimed to be present where AM cases were reported in the US. For unknown reasons, occurrence of this disease has increased. It is important to define environmental key factors that may influence toxicity of samaras from Acer species. In addition, the content of HGA in seedlings needs to be determined since AM outbreaks, during autumn period when the seeds fall but also during spring when seeds are germinating. The present study aims to validate a reliable method using high performance thin layer chromatography for determination and comparison of HGA in samaras and seedlings. The working range of the method was between 20 μg HGA to 408 μg HGA per ml water, corresponding to 12 - 244 mg/kg fresh weight or 40 - 816 mg/kg dry weight, taking into account of an arbitrary average dry matter content of 30%. Instrumental limit of detection and limit of quantification were of 10 µg HGA/ml and 20 µg HGA/ml water, respectively. Instrumental precision was 4% (RSD on 20 repeated measurements) while instrumental accuracy ranged between 86% and 121% of expected value. The HGA recovery of the analytical method estimated from spiked samaras and seedlings samples ranged between 63 and 103%. The method was applied to 9 samples of samaras from Acer pseudoplatanus, A. platanoides and A. campestre and 5 seedlings samples from A. pseudoplatanus. The results confirm detection of HGA in samaras from A. pseudoplatanus and the absence of detection in samaras of other tested species. They also suggest that detected levels of HGA are highly variable. This confirmed the suitability of the method for HGA detection in samaras or seedling. [less ▲]

Detailed reference viewed: 43 (10 ULiège)
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See detailDevelopment of an immobilisation device for treatment of patients after breast conserving surgery for mammary carcinoma.
COUCKE, Philippe ULiege; WONNER, Michel ULiege; JANVARY, Zsolt Levente ULiege et al

Poster (2010, September)

To develop an immobilisation device for prone positioning for breast irradiation. The basic requirements for the device are: rapid and accurate patient (re)positioning, compatibility with modern imaging ... [more ▼]

To develop an immobilisation device for prone positioning for breast irradiation. The basic requirements for the device are: rapid and accurate patient (re)positioning, compatibility with modern imaging devices and easy to handle by the treating technologists. [less ▲]

Detailed reference viewed: 38 (0 ULiège)
Peer Reviewed
See detailDevelopment of an implantable integrated MEMS pressure sensor using polyimide epoxy composite and Atomic Layer Deposition for encapsulation
Gembaczka, P; Görtz, M; Kraft, Michael ULiege

in Proceedings of "48th DGBMT Annual Conf. : Hannover, Germany Octobre 2014" (2014, October)

Detailed reference viewed: 24 (0 ULiège)
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See detailDevelopment of an in vitro culture method for heart-shaped embryo in Phaseolus vulgaris.
Mergeai, Guy ULiege; Baudoin, Jean-Pierre ULiege

in Annual report of the Bean Improvement Cooperative (1990), 33

Detailed reference viewed: 5 (2 ULiège)
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See detailDevelopment of an in vitro culture method of very immature embryos to assist wide interspecific hybridisation in Phaseolus.
Geerts, P.; Baudoin, Jean-Pierre ULiege; Mergeai, Guy ULiege

in 3rd European conference on grain legumes : Opportunities for high quality, healthy and added-value crops to meet European demands, 14-19 November 1998, Valladolid, Spain (1998)

Detailed reference viewed: 11 (6 ULiège)
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See detailDevelopment of an in vitro culture techniques adapted to Phaseolus polyanthus and P. vulgaris embryos.
Geerts, P.; Baudoin, Jean-Pierre ULiege; Mergeai, Guy ULiege

in Bean Improvement Cooperative (1998), 41

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See detailDevelopment of an in vitro endotoxin test for monoolein-water liquid crystalline gel for use as an implant
Ouédraogo, Moustapha; Semdé, Rasmané; Somé, Issa T. et al

in Tropical Journal of Pharmaceutical Research [=TJPR] (2009), 8(6), 501-508

Purpose : Drugs that are administered by parenteral route must be apyrogenic. The aim of this study was to develop an in vitro endotoxin test for liquid crytalline gels for use as implants, using a ... [more ▼]

Purpose : Drugs that are administered by parenteral route must be apyrogenic. The aim of this study was to develop an in vitro endotoxin test for liquid crytalline gels for use as implants, using a monoolein-water liquid crystalline gel as a model. Methods : The gel-clot technique was used. The gel was dissolved first in isopropyl myristate, and the endotoxins were extracted with water for bacterial endotoxin test. Tests for the labelled lysate sensitivity and interfering factors were performed to validate the developed method. The limit of detection of endotoxin in the gel was also determined. Results : The labelled lysate sensitivity was confirmed. It was not influenced by the presence of extracts from the gels. Endotoxins in the contaminated test gels were completely extracted. Endotoxin concentration in the tested gels was below the calculated threshold endotoxin level. Conclusion : A method to perform in vitro endotoxins test of liquid crystalline gels was successfully developed and validated. Application of the technique to gels currently being developed in our laboratories indicate that the gels were apyrogenic. [less ▲]

Detailed reference viewed: 40 (5 ULiège)
See detailDevelopment of an in vitro model for mercury exposure in marine mammals
Dupont, Aurélie ULiege; Bouquegneau, Jean-Marie ULiege; Siebert, Ursula et al

Poster (2007, November 01)

Detailed reference viewed: 19 (5 ULiège)
Peer Reviewed
See detailDevelopment Of An In Vitro Pod Culture Technique For Young Pods Of Phaseolus Vulgaris L.
Geerts, P.; Sassi, K.; Mergeai, Guy ULiege et al

in In Vitro Cellular & Developmental Biology-Plant (2000), 36(6),

Detailed reference viewed: 17 (5 ULiège)
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See detailDevelopment of an in vivo phosphorylation system in Escherichia coli
Goux, Marine ULiege

Poster (2013, October)

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as zirconium-89 (89Zr, t½=78.4h) or gallium-68 (68Ga, t½=1.13h)[a ... [more ▼]

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as zirconium-89 (89Zr, t½=78.4h) or gallium-68 (68Ga, t½=1.13h)[a]. Despite their important role in the therapeutic world, antibodies have many disadvantages related to their structure. In addition to their limited stability, the presence of disulfide bridges and their post-translational modifications make their production difficult and expensive. Moreover, conjugation of chelating agent often occurs on lysines, which is non-regioselective and leads to a heterogeneous mixture of products. In addition, the long clearance of antibodies can be a problem to obtain a good contrast when they are used in imaging. To address these different limitations, we developed a chemistry-free chelating system consisting of a highly phosphorylatable peptide tag. A specific phosphorylation step, with the alpha subunit of the casein kinase II (CKIIα), generates a nanocluster of 4 phosphates that can interact strongly with metal ion like zirconium. This strategy has already demonstrated its powerfulness for the stable and specific anchoring of protein on zirconium phosphonate-based microarray[b]. We are now adapting the use of this labeling tag to the stereoselective chelation of radionuclides for PET imaging. To ensure an efficient targeting of the radionuclide, the tag was genetically fused to a Nanofitin®, a protein scaffold developed as an alternative to antibodies. Nanofitins share the advantages of being small (7KDa), cystein-free, easy to engineer, highly stable both to temperature and pH and expressed with high yield in Escherichia coli. As described previously, functionalizing the tag with a phosphate nanocluster is a key step to provide it with the capacity to specifically chelate metal ions such as zirconium or gallium. In early development the phosphorylation was realized in vitro. In order to save purification steps and decrease production costs, we developed an in vivo phosphorylation system consisting in the co-expression of a Nanofitin® fused to the phosphorylatable tag and the CKIIα. Effectiveness of the in vivo system was demonstrated together by electrophoretic mobility shift assay and staining with a specific phosphoprotein staining gel: ProQDiamond®. To further optimize and validate the system, the rate of phosphorylation is evaluated by an isoelectric focusing method. This technique is based on the fact that the pHi of the Nanofitin® decreases as the protein is phosphorylated. Such a technique allowed us to isolate the different phosphorylation states of the phospho-Nanofitin® (from 0 to 4 phospho-serine). In parallel to the development of the in vivo system, we are developing a phosphoprotein purification protocol by immobilized metal affinity chromatography, relying on the specific interaction of phosphate moeity with gallium(III) or iron(III). The use of a single tag for both the purification and the labeling will provide a much simpler and straightforward system. Finally this fusion system of phosphate nanocluster and a Nanofitin® will allows us to combine the advantages of an original single step regioselective labeling for both zirconium-89 and gallium-68, together with a highly stable targeting moiety. [a] Heuveling D. A. et al. (2011), Oral Oncology, 47, pp. 2–7 [b] Cinier M. et al. (2012), Journal of Biological Inorganic Chemistry, 17, pp. 399–407 [less ▲]

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See detailDevelopment of an integrated modelling methodology for compartment fires by implementing a weak coupling strategy between a CFD and an FE software
Tondini, Nicola; Franssen, Jean-Marc ULiege

in De Luca, Antonello (Ed.) XXIII giornate italiane della costruzione in acciaio (2011, October 12)

This article presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a ... [more ▼]

This article presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a finite element (FE) software applied to structural systems. In particular, a weak coupling methodology use to simulate a fire exposed structure by modelling the fire development in the compartment, the heat penetration in the structure and the mechanical response is described. The advantages and the disadvantages of such a technique are highlighted compared to a full coupling that conversely takes into account all mutual interactions. This methodology is particularly suitable for relatively thin steel structures in large compartments. Moreover, the need of a standardised transfer file in order to get the methodology as flexible as possible in term of versatility is emphasized. [less ▲]

Detailed reference viewed: 42 (1 ULiège)
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See detailDEVELOPMENT OF AN INTEGRATED STRATEGY FOR CONTROLLING THE ALLERGEN ISSUE IN THE BELGIAN FOOD AND CATERING INDUSTRY - ALLERRISK
De Loose, Marc; Daeseleire, Else; Taverniers, Isabelle et al

Report (2009)

Detailed reference viewed: 36 (1 ULiège)
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See detailDevelopment of an integrated tool for solar buildings design and renewal.
Andre, Philippe ULiege; Nicolas, Jacques ULiege

in Proceedings PLEA 96 International conference (1996, September)

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See detailDevelopment of an interface between a CFD software and a FE software for compartment fires
Tondini, Nicola; Franssen, Jean-Marc ULiege

in Costruzioni Metalliche (2012), 3

The article presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a ... [more ▼]

The article presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a Finite Element (FE) software applied to structural systems. In particular, a weak coupling methodology used to simulate a fire exposed structure by modelling the fire development in the compartment, the heat penetration in the structure and the mechanical response is described. A practical application is also shown. [less ▲]

Detailed reference viewed: 66 (1 ULiège)
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See detailDevelopment of an interface between CFD and FE software
Tondini, Nicola; Vassart, Olivier; Franssen, Jean-Marc ULiege

in Fontana, Mario; Frangi, Andrea; Knobloch, Markus (Eds.) Proceedings of the 7th International Conference on Structures in Fire (2012, June 06)

The paper presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a ... [more ▼]

The paper presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a Finite Element (FE) software applied to structural systems. In particular, a weak coupling approach used to simulate a fire exposed structure by modelling the fire development in the compartment, the heat penetration in the structure and the mechanical response is described. The advantages and the disadvantages of such a technique are highlighted compared to a full coupling that conversely takes into account all mutual interactions. The favourable aspect of computing the thermal response of the structure in the FE model in order to avoid modelling the structure in the CFD model is underlined, namely a sensitive reduction of computational demand. Finally, a practical application is presented. [less ▲]

Detailed reference viewed: 129 (2 ULiège)
See detailDevelopment of an Interferometric Mass Processing Chain for Multitemporal Ground Deformation Measurements
Libert, Ludivine ULiege; De Rauw, Dominique ULiege; d'Oreye, Nicolas

Conference (2017, November 07)

The main goal of the RESIST project is to understand the mechanisms driving volcanic eruptions and landslides in the Kivu region, on the East African Rift. In order to model both volcanic and landslide ... [more ▼]

The main goal of the RESIST project is to understand the mechanisms driving volcanic eruptions and landslides in the Kivu region, on the East African Rift. In order to model both volcanic and landslide processes, it is necessary to measure ground deformations in the region accurately. For this purpose, both ground-based instruments (e.g. GPS network) and spaceborne data (e.g. optical and SAR images) are used. One aspect of the project focuses on Differential SAR Interferometry (DInSAR), which is a technique used to map ground deformations occurring between two SAR images acquired at different times. It is today a well-mastered technique that offers large spatial coverage with a typical temporal sampling of one to several days, depending on the chosen sensor. In the last years, multitemporal approaches based on DInSAR have been developed, like the Small BAseline Subset (SBAS) [1] and Multidimensional Small BAseline Subset (MSBAS) [2] techniques, or the Persistent Scatterers Interferometry (PSI) [3]. In the framework of RESIST, we use MSBAS to perform ground deformations monitoring along time. The Multidimensional Small BAseline Subset (MSBAS) technique produces 2-D time series of ground deformations by integrating data sets of SAR images acquired by different sensors, with different spatial and temporal sampling, resolutions, incidence angles, wavelengths, pass directions and other varying parameters. By combining at least two data sets with overlapping spatial and temporal coverage and an extended range of look angles, the evolution of deformations in the vertical and west-east directions can be computed by the MSBAS approach [2]. The MSBAS software feeds on a large amount of deformation maps, which are produce by DInSAR. Such an amount of data cannot be produced by hand and that is the reason why we developed an automatic interferometric processing chain meant to produce large amounts of products adequate for multitemporal methods like MSBAS. The interferometric processing is supported by the CSL InSAR Suite (CIS) software developed at Centre Spatial de Liège, which presents the advantages to be fully adaptable to the needs of the MSBAS technique and the thematic specificities. Indeed, numerous options (e.g. adaptive filtering, wide swath interferometry) have been added to the CIS software in the framework of the RESIST project. In a first time, we will briefly present the MSBAS approach and its advantages regarding the ground deformation measurements. In the second part of the presentation, we will introduce the mass processing chain step by step and its functionalities. Critical steps of the chain, like the chosen strategy for the interferometric pairs selection, the integration of Sentinel-1 data or the image interpolation approach, will be presented in more details. Finally, preliminary results of an MSBAS processing over the Bukavu area will be presented. REFERENCES: [1] Berardino, P., Fornaro, G., Lanari, R., and Sansosti, E. (2002). A new algorithm for surface deformation monitoring based on small baseline differential SAR interferograms, IEEE Trans. Geosci. Remote Sens., 40, 11, pp. 2375-2383. doi: 10.1109/TGRS.2002.803792. [2] Samsonov, S., and d’Oreye, N. (2012). Multidimensional time series analysis of ground deformation from multiple InSAR data sets applied to Virunga Volcanic Province, Geophysical Journal International, 191, 3, pp. 1095-1108. doi: 10.1111/j.1365-246X.2012.05669.x [3] Ferretti, A., Prati, C., and Rocca, F. (2001). Permanent scatterers in SAR interferometry, IEEE Trans. Geosci. Remote Sens., 39, 1, pp. 8-20. doi: 10.1109/36.898661. [less ▲]

Detailed reference viewed: 39 (3 ULiège)
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See detailDevelopment of an interspecific breeding programme in cotton assisted by RAPD markers
Mergeai, Guy ULiege; Vroh Bi, I.; du Jardin, Patrick ULiege et al

in Cotton biotechnology (1995)

Detailed reference viewed: 22 (4 ULiège)