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See detailDevelopment of a Quick Quantitative Real-Time PCR for the In Vivo Detection and Quantification of Peach latent mosaic viroid
Parisi, Olivier ULiege; Lepoivre, Philippe ULiege; Jijakli, Haissam ULiege

in Plant Disease (2011), 95(2), 137-142

Viroids are plant pathogens infecting a broad range of herbaceous and tree crops. Among them, the Peach latent mosaic viroid (PLMVd) infects mainly peach trees, causing a loss of production with no ... [more ▼]

Viroids are plant pathogens infecting a broad range of herbaceous and tree crops. Among them, the Peach latent mosaic viroid (PLMVd) infects mainly peach trees, causing a loss of production with no curative options. Detecting this viroid is thus important for certification procedures aiming to avoid the release of infected material into orchards. Presented here is a complete detection method based on reverse transcription (RT) followed by a quantitative real-time polymerase chain reaction (PCR). New primers were selected and optimal reaction conditions determined for routine application of the method. The technique is 105 times more sensitive than the endpoint RT-PCR used for PLMVd detection, and permits earlier detection of PLMVd in infected plants. The quick, low-cost extraction procedure used and the quality of the results obtained make this method suitable for routine testing. [less ▲]

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See detailDevelopment of a radioimmunoassay for bovine chymosin B
Idrissa-Sidikou, Djibo; Remy, Benoit; Dufrasne, Isabelle ULiege et al

in Tropicultura (2007), 25(3), 134-139

The present study was conducted to develop and validate a specific radioimmunoassay system for measurement of bovine chymosin B (bChyB) concentrations in plasma samples. Bovine ChyB was used for ... [more ▼]

The present study was conducted to develop and validate a specific radioimmunoassay system for measurement of bovine chymosin B (bChyB) concentrations in plasma samples. Bovine ChyB was used for immunization of rabbits and as standard and tracer. Chymosin B concentrations were measured in plasma samples from two groups of calves (Group 1: calves sampled from birth to 24 hours; Group 2: calves sampled from Day 1 to 21 after birth) and from one cow during the peri-partum period. Detection limit of the assay was 9.0 ng/mL. Recovery was higher than 89.3%. Repeatability and reproducibility ranged from 1.52% to 5.23% and from 1.52% to 12.57% respectively. No cross-reaction was found with pepsinogen A from bovine, porcine or human origins. In Group 1, bChyB concentrations increased from 47.3±45.1 ng/mL (5 min after birth) to 325.5±161.2 ng/mL (12 hours after birth), then it decreased till 293.0±161.5 ng/mL (24 hours after birth). In Group 2, concentrations decreased from Day 1 (455.3±191.1 ng/mL) to Day 21 (117.9±85.1 ng/mL). In adult cow, mean concentration was 136.0±32.3 ng/mL. In conclusion, bChyB is able to cross the stomach basal membrane and to reach the blood circulation at detectable levels in both young calves and adult cows. [less ▲]

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See detailDevelopment of a Radioimmunoassay for Bovine Pepsinogen A
Sidikou, I. D.; Remy, B.; Beckers, Jean-François ULiege

in Revue d'Elévage et de Médecine Vétérinaire des Pays Tropicaux (2005), 58(4), 229-235

Pepsinogen A is the most abundant zymogen found in blood, and its enzymatic measurement is used for the diagnosis of gastric lesions. The present study was conducted to develop a radioimmunoassay (RIA ... [more ▼]

Pepsinogen A is the most abundant zymogen found in blood, and its enzymatic measurement is used for the diagnosis of gastric lesions. The present study was conducted to develop a radioimmunoassay (RIA) specific to pepsinogen A in bovine plasma. The authors purified large amounts of three non-denatured isoforms of bovine pepsinogen A with high proteolytic activity. These homogeneous preparations were used to produce specific antisera in New Zealand White rabbits, and three antisera with high titers were obtained. In the present assay the antiserum #822 was used at a final dilution of 1/250,000. The detection limit of the assay was 20 ng/ml and the recovery ranged from 85.5 to 103.3%. The repeatability (intra-assay coefficient of variation) was lower than 6.6%, whereas the reproducibility (inter-assay coefficient of variation) was lower than 13.4%. The capacity of the RIA to detect pepsinogen A in blood was tested by measuring the concentrations in plasma of newborn calves (n = 6) serially sampled from birth to four months of age. The mean pepsinogen A value (mean ± standard deviation) in the plasma of calves was 2071 ± 752 ng/ml one day after birth. The concentration decreased progressively and was about 1196 ± 307 ng/ml at day 21, and 677 ± 109 ng/ml at day 120. The present study is the first report on pepsinogen A concentrations in bovine measured by RIA. Further investigations using the RIA should be performed in order to confirm these values and determine pepsinogen levels in older cattle in physiological and pathological conditions such as gastrointestinal helminthosis [less ▲]

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See detailDevelopment of a RAPD marker and a semi-selective medium for aureobasidium pullulans (strain Ach1-1), a biocontrol agent against postharvest disease on apples
El Hamouchi, A.; Najimi, B.; Achbani, E. H. et al

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (2007), 30

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See detailDevelopment Of A Rapid Rt-Pcr Test For The Detection Of Peach Latent Mosaic Viroid, Pear Blister Canker Viroid, Hop Stunt Viroid And Apple Scar Skin Viroid In Fruit Trees From Tunisia
Hassen, If.; Roussel, S.; Kummert, J. et al

in Journal of Phytopathology (2006), 154(4), 217-223

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See detailDevelopment of a Reference Database for Particle-Induced Gamma-ray Emission spectroscopy
Dimitriou, P.; Becker, H.-W.; Bogdanović-Radović, I. et al

in Nuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms (2016), 371

Particle-Induced Gamma-ray Emission (PIGE) is a powerful analytical technique that exploits the interactions of rapid charged particles with nuclei located near a sample surface to determine the ... [more ▼]

Particle-Induced Gamma-ray Emission (PIGE) is a powerful analytical technique that exploits the interactions of rapid charged particles with nuclei located near a sample surface to determine the composition and structure of the surface regions of solids by measurement of characteristic prompt γ rays. The potential for depth profiling of this technique has long been recognized, however, the implementation has been limited owing to insufficient knowledge of the physical data and lack of suitable user-friendly computer codes for the applications. Although a considerable body of published data exists in the nuclear physics literature for nuclear reaction cross sections with γ rays in the exit channel, there is no up-to-date, comprehensive compilation specifically dedicated to IBA applications. A number of PIGE cross-section data had already been uploaded to the Ion Beam Analysis Nuclear Data Library (IBANDL) (http://www-nds.iaea.org/ibandl) by members of the IBA community by 2011, however a preliminary survey of this body of unevaluated experimental data has revealed numerous discrepancies beyond the uncertainty limits reported by the authors. Using the resources and coordination provided by the IAEA, a concerted effort to improve the situation was made within the Coordinated Research Project on the Development of a Reference Database for PIGE spectroscopy, from 2011 to 2015. The aim of the CRP was to create a data library for Ion Beam Analysis that contains reliable and usable data on charged particle γ-ray emission cross sections that would be made freely available to the user community. As the CRP has reached its completion, we shall present its main achievements, including the results of nuclear cross-section evaluations and the development of a computer code that will become available to the public allowing for the implementation of a standardless PIGE technique. © 2015 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 31 (4 ULiège)
See detailDevelopment of a regenerable filter aid for beverages
Bonacchelli, B.; Zgoulli, S.; Harmegnies, F. et al

Poster (1998, May 08)

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See detailDevelopment of a regenerable filtration aid for beverages.
Bonacchelli, B.; Zgoulli, S.; Harmegnies, F. et al

Poster (1997, August)

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See detailDevelopment of a regional-scale library of near infrared reflectance soil spectra for alternative assessment of soil characteristics in Southern Belgium
Genot, Valérie ULiege; Dardenne, Pierre; Bock, Laurent ULiege et al

Poster (2012)

In Walloon Region (Southern Belgium), five routine soil laboratories are grouped within a network promoting a better quality in analysis (www.requasud.be). The harmonization of protocols as well as ... [more ▼]

In Walloon Region (Southern Belgium), five routine soil laboratories are grouped within a network promoting a better quality in analysis (www.requasud.be). The harmonization of protocols as well as methodological or technical prospective are realized under scientifically supervision of our research laboratory. In this context, a study was conducted to evaluate the ability of the NIRS to predict some soil properties: CEC, TOC, TN and clay content. The initial models were elaborated upon local PLS regression on set of 1 300 soil samples. The local PLS calibration used allows an accurate prediction of the soil properties and precision of NIRS technique is comparable to reference analytical method [less ▲]

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See detailThe development of a safety culture: a fanciful hope in the health care sector ?
COUCKE, Philippe ULiege

in Belgian Journal of Medical Oncology [=BJMO] (2014), 8(3), 66-71

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See detailDevelopment Of A Scar Marker And A Semi-Selective Medium For Specific Quantification Of Pichia Anomala Strain K On Apple Fruit Surfaces
De Clercq, Deborah; Cognet, Stephane; Pujol, Marta et al

in Postharvest Biology & Technology (2003), 29(3), 237-247

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See detailDevelopment of a scoring system to assess lameness status in dairy cattle farms
Frisee, Vincent ULiege; Guyot, Hugues ULiege

in Société Belge Francophone de Buiatrie & Vlaamse vereniging voor Buiatrie (Ed.) Proceeding of the 6th European Congress of Bovine Health Management (2011, September)

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See detailDEVELOPMENT OF A SCROLL EXPANDER FOR MICRO-CSP WITH ORGANIC RANKINE CYCLE
Dechesne, Bertrand ULiege; Orosz, M.S.; Legros, Arnaud ULiege et al

in Dechesne, Bertrand; Orosz, M.S.; Legros, Arnaud (Eds.) et al DEVELOPMENT OF A SCROLL EXPANDER FOR MICRO-CSP WITH ORGANIC RANKINE CYCLE (2012)

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See detailDevelopment of a self-administrated quality of life questionnaire for sarcopenia in elderly subjects: the SarQol
Beaudart, Charlotte ULiege; Biver, Emmanuel; Reginster, Jean-Yves ULiege et al

in Age & Ageing (2015), 44

BACKGROUND: the impact of sarcopenia on quality of life is currently assessed by generic tools. However, these tools may not detect subtle effects of this specific condition on quality of life. OBJECTIVE ... [more ▼]

BACKGROUND: the impact of sarcopenia on quality of life is currently assessed by generic tools. However, these tools may not detect subtle effects of this specific condition on quality of life. OBJECTIVE: the aim of this study was to develop a sarcopenia-specific quality of life questionnaire (SarQoL, Sarcopenia Quality of Life) designed for community-dwelling elderly subjects aged 65 years and older. SETTINGS: participants were recruited in an outpatient clinic in Liège, Belgium. SUBJECTS: sarcopenic subjects aged 65 years or older. METHODS: the study was articulated in the following four stages: (i) Item generation-based on literature review, sarcopenic subjects' opinion, experts' opinion, focus groups; (ii) Item reduction-based on sarcopenic subjects' and experts' preferences; (iii) Questionnaire generation-developed during an expert meeting; (iv) Pretest of the questionnaire-based on sarcopenic subjects' opinion. RESULTS: the final version of the questionnaire consists of 55 items translated into 22 questions rated on a 4-point Likert scale. These items are organised into seven domains of dysfunction: Physical and mental health, Locomotion, Body composition, Functionality, Activities of daily living, Leisure activities and Fears. In view of the pretest, the SarQoL is easy to complete, independently, in ∼10 min. CONCLUSIONS: the first version of the SarQoL, a specific quality of life questionnaire for sarcopenic subjects, has been developed and has been shown to be comprehensible by the target population. Investigations are now required to test the psychometric properties (internal consistency, test-retest reliability, divergent and convergent validity, discriminant validity, floor and ceiling effects) of this questionnaire. [less ▲]

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See detailDevelopment of a self-administrated quality of life questionnaire specific to sarcopenia in elderly aged 65 and older : the SarQol
Beaudart, Charlotte ULiege; Reginster, Jean-Yves ULiege; Rizzoli, R et al

in Journal of Frailty & Aging (2015), 4(S1), 31-32

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See detailDevelopment of a semi-analytical model of volumetric expander for system-level simulation.
Oudkerk, Jean-François ULiege

in Proceedings of ECOS 2016 (2016, June)

Organic Rankine cycles are particularly well suited for recovering energy from low-grade heat sources, such as industrial waste, engine exhaust gas or solar concentrated power. In ow-capacity systems ... [more ▼]

Organic Rankine cycles are particularly well suited for recovering energy from low-grade heat sources, such as industrial waste, engine exhaust gas or solar concentrated power. In ow-capacity systems, volumetric expanders are often preferred to turbomachines because of their reliability, robustness, low rotational speed and their ability to handle high pressure ratios… Different types of machines have been investigated and successfully tested by the uthors and tools have been developed in order to analyse experimental data. Semi-empirical odel was built to analyse losses, detect potential improvement and simulate performances. This paper present a simple and fastcomputing semi-analytical model useful for simulate dynamic system integrating volumetric expander. This model is validated on experimental data and compare to black-box model. [less ▲]

Detailed reference viewed: 34 (5 ULiège)
See detailDevelopment of a semiochemical-based control method against the walnut husk fly
Sarles, Landry ULiege; Heuskin, Stéphanie ULiege; Verheaghe, Agnès et al

Conference (2016, May 17)

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