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See detailEquine glaucomas: A review
Ollivier FJ; Sanchez RF; Monclin, Sébastien ULg

in Equine Veterinary Education (2009), 21(5), 232-235

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See detailEquine joint assessment: the role of ultrasound
Busoni, Valeria ULg

Conference (2008, August)

The presentation was a key-note talk about the use of ultrasonography in the assessment of equine joints.

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See detailAn equine joint friction test model using a cartilage-on-cartilage arrangement.
Noble, Prisca ULg; Collin, Bernard ULg; Lecomte-Beckers, Jacqueline ULg et al

in Veterinary Journal (2010)

This study describes an equine joint friction test using a cartilage-on-cartilage arrangement and investigates the influence of age and load on the frictional response. Osteochondral plugs were extracted ... [more ▼]

This study describes an equine joint friction test using a cartilage-on-cartilage arrangement and investigates the influence of age and load on the frictional response. Osteochondral plugs were extracted from equine shoulder joints (2-5 years, n=12; 10-14 years, n=15), and mounted in a pin-on-disc tribometer. The frictional response was then measured under constant conditions (2N; 20 degrees C; 5 mm/s), and with increasing load (2N, 5N, 10N). In all experiments, the friction coefficient of young cartilage was significantly (P<0.001) smaller than obtained from old cartilage, while the application of a greater load resulted in a significant (P<0.001) decrease in friction coefficient only in old cartilage. It was concluded that cartilage ageing was responsible for an increase in friction coefficient under these experimental conditions. Moreover, where young cartilage lubrication remained stable, cartilage ageing may have been responsible for lubrication regime change. The cartilage-on-cartilage model could be used to better understand lubrication regime disturbances in healthy and diseased equine joints, and to test the efficacy of various bio-lubricant treatments. [less ▲]

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See detailEquine lung scintigraphy
Votion, Dominique ULg; Lekeux, Pierre ULg

in Rantanen, N. W.; Hauser, M. L. (Eds.) Dubai International Equine Symposium : the diagnosis and treatment of respiratory disease (1997, April)

Diagnostic imaging of lung disease using radioactive tracers is a well-established procedure in human nuclear medicine. Conversely to other imaging techniques, gamma scintigraphy explores the functional ... [more ▼]

Diagnostic imaging of lung disease using radioactive tracers is a well-established procedure in human nuclear medicine. Conversely to other imaging techniques, gamma scintigraphy explores the functional and metabolic characteristics of an organ or a system rather than its morphology. Compared to other pulmonary function tests, scintigraphy provides regional evaluation instead of global measurement of lung function. For these reasons, this non invasive technique is a powerful tool for the study and diagnosis of respiratory disorders, as well as in the development of their treatment. In equine medicine the techniques to perform and analyse scintigraphic lung images have been described. Equine lung scintigraphy emerges as a diagnostic and research tool9. This paper describes the scintigraphic technique, the method for analysis of equine lung scintiscans, and reviews the potential application of equine lung scintigraphy. [less ▲]

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See detailEquine myeloperoxidase: A novel biomarker in synovial fluid for the diagnosis of infection.
Wauters, J.; Pille, F.; Martens, A. et al

in Equine Veterinary Journal (2013), 45(3),

REASONS FOR PERFORMING STUDY: Equine joint infection is a life-threatening disorder, and confirmation of the diagnosis can be difficult. Synovial fluid biomarkers may assist the discrimination between ... [more ▼]

REASONS FOR PERFORMING STUDY: Equine joint infection is a life-threatening disorder, and confirmation of the diagnosis can be difficult. Synovial fluid biomarkers may assist the discrimination between infectious and noninfectious joint disease. OBJECTIVES: This study investigates whether the immunological detection of total and enzymatically active myeloperoxidase (MPO) assists the diagnosis of joint infection in horses. METHODS: The following 4 sample groups were included: healthy; osteochondritis dissecans (OCD); traumatic synovitis; and culture-confirmed infected joints. Synovial fluid was analysed for total MPO by a horse-specific sandwich enzyme-linked immunosorbent assay (ELISA) and for active MPO using the specific immunological extraction followed by enzymatic detection (SIEFED) technique. Western blot analysis was performed to confirm the antibody specificity. RESULTS: Synovial fluid from infected joints contained significantly more total and active MPO than samples from healthy joints, joints affected by OCD and joints with traumatic synovitis. Cut-off values were set at 5000 and 350 ng/ml for total and active MPO, respectively, with fair sensitivity, specificity, positive and negative predictive values and likelihood ratios for infection. Correlation coefficients were reported between the total as well as the active MPO levels and the routine synovial fluid parameters, i.e. the white blood cell count, the neutrophil count and the total protein level. No correlation was observed between MPO and either the age of the horse or the joint affected. Western blotting confirmed the antibody specificity for equine MPO. CONCLUSIONS AND POTENTIAL RELEVANCE: Synovial fluid MPO was identified as a very promising biomarker to augment the discrimination of infectious vs. noninfectious joint disease in horses. Both ELISA and SIEFED techniques can be used for its specific and rapid detection. The analysis of synovial fluid MPO can be used as a complementary test to aid in the discrimination between infectious and noninfectious joint disease, especially when the white blood cell counts and the total protein level are inconclusive. [less ▲]

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See detailEquine neutrophil elastase in plasma, laminar tissue, and skin of horses administered black walnut heartwood extract.
de la Rebière de Pouyade, Geoffroy ULg; Riggs, L. M.; Moore, J. N. et al

in Veterinary immunology and immunopathology (2009)

Laminitis is a local manifestation of a systemic inflammatory response that is characterized by neutrophil activation and movement of neutrophils into the laminar tissues. Given the evidence for the ... [more ▼]

Laminitis is a local manifestation of a systemic inflammatory response that is characterized by neutrophil activation and movement of neutrophils into the laminar tissues. Given the evidence for the involvement of neutrophils in the development of laminitis, we measured concentrations of neutrophil elastase, a serine protease released from the azurophilic granules of neutrophils, in plasma, skin and laminar tissues obtained from control horses and horses given black walnut heartwood extract (BWHE) to induce laminitis. Healthy horses (5-15 years old) were randomly assigned to 4 groups: 3 experimental groups given BWHE via nasogastric tube, and a control group given an equal volume of water. The experimental groups consisted of horses euthanized 1.5h (n=5), 3h (n=6) or 12h (n=10) after BWHE administration. Control horses (n=7) were euthanized 12h after intragastric administration of water. Plasma samples were collected in all horses of the control and 12h BWHE groups at 0, 1, 2, 3, 4, 6, 8, 10, and 12h after treatment, and laminar tissue and skin from the middle region of the neck were harvested at the time of euthanasia in all 1.5 and 3h BWHE horses, in 6 of the 12h BWHE horses and in 5 of the control horses. Plasma and tissue concentrations of neutrophil elastase were determined using an equine specific ELISA, and statistical significance was set at p<0.05. Plasma concentrations of neutrophil elastase in the BWHE group were significantly higher at 6 and 8h compared to the control group and at 8 and 10h compared to time 0. Concentrations of neutrophil elastase in skin and laminar tissue were significantly higher in the 3 and 12h BWHE groups compared to the control group. Concentrations of neutrophil elastase were significantly higher in the skin than in the lamina in the 12h BWHE horses. The administration of BWHE thus results in significant increases in the concentration of neutrophil elastase in the circulation, skin and laminar tissue. These results confirm a role for neutrophils in the developmental phase of laminitis, and the systemic nature of the inflammatory process. Furthermore, neutrophil elastase may play a key role in the disintegration of the hoof basal membrane and be a target for the development of new treatments for laminitis. [less ▲]

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See detailEquine neutrophil myeloperoxidase in plasma: design of a radio-immunoassay and first results in septic pathologies.
Deby, Ginette ULg; Grulke, Sigrid ULg; Caudron, I. et al

in Veterinary Immunology and Immunopathology (1998), 66(3-4), 257-71

The strangulated intestinal pathologies of horses are accompanied by a local activation of the neutrophils, that can be revealed by measuring the tissular enzymatic activity of the granulocytic enzyme ... [more ▼]

The strangulated intestinal pathologies of horses are accompanied by a local activation of the neutrophils, that can be revealed by measuring the tissular enzymatic activity of the granulocytic enzyme myeloperoxidase (MPO). To estimate the possible spreading of this neutrophil activation to the systemic circulation, we designed a radioimmunoassay (RIA) for equine neutrophil myeloperoxidase (MPO) (EC 1.11.1.7) using a specific rabbit antiserum. MPO was labeled with 1 mCi 125I by a technique of self-labeling in the presence of 10(-4) M hydrogen peroxide. The RIA was performed by incubation of 100 microl diluted antiserum, 100 microl labeled MPO (+/-30,000 cpm) and 100 microl of the reference molecule (unlabeled MPO) solution or the unknown sample, at room temperature for 18 h. The antibody-antigen complexes were isolated by double antibody precipitation. The sensitivity of the RIA was 2 ng/ml. The RIA showed good precision and accuracy with intra- and inter-assay coefficients of variation 6% and 8%, respectively, for MPO concentrations ranging from 2 ng/ml to 60 ng/ml. The best sampling technique for MPO measurement in plasma was to collect blood into EDTA, which allowed us to get a plasmatic value stable with time. The mean MPO value in normal horses was 69.5 +/- 19.4 ng/ml in EDTA anticoagulated plasma (n = 48). The stress of transport and anaesthesia did not modify the mean plasmatic value of MPO. No significant increase of plasma MPO was observed in 17 horses submitted to surgery for pathologies without systemic impact. But, in 25 horses with obstructive intestinal pathologies, persistent abnormal MPO concentrations were measured (until 740 ng/ml). [less ▲]

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See detailEquine oesophageal obstruction : a retrospective study on 143 cases
Seguin, A; Peters, F; Lopez, D et al

in Proceedings of the 45th Congress of the British Equine Veterinary Association (2006)

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See detailEquine Osteochondrosis and possible relation with plasma IGF-I levels
Verwilghen, Denis ULg

Master of advanced studies dissertation (2006)

Detailed reference viewed: 24 (6 ULg)
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See detailEquine Piroplasmosis in Belgium: a retrospective study.
Delguste, Catherine ULg; Mantran, A.; Sandersen, Charlotte ULg et al

Poster (2006, January)

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See detailEquine Post Anaesthetic Myositis: Muscular Post Ischaemic Hyperaemia Measured by Laser Doppler Flowmetry
Serteyn, Didier ULg; Lavergne, L.; Coppens, P. et al

in Veterinary Record : Journal of the British Veterinary Association (1988), 123(5), 126-8

Measurements of muscular microcirculation in horses anaesthetised with halothane were performed by laser Doppler flowmetry. Variations of microcirculation in the compressed and uncompressed triceps ... [more ▼]

Measurements of muscular microcirculation in horses anaesthetised with halothane were performed by laser Doppler flowmetry. Variations of microcirculation in the compressed and uncompressed triceps brachii were measured when horses were positioned in dorsal recumbency after a prolonged period in lateral recumbency. A significant post ischaemic hyperaemia was recorded in horses which developed a post anaesthetic myositis. [less ▲]

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See detailEquine Postanaesthetic Myositis: A Possible Role for Free Radical Generation and Membrane Lipoperoxidation
Serteyn, Didier ULg; Mottart, E.; Deby-Dupont, G. et al

in Research in Veterinary Science (1990), 48(1), 42-6

A method for the evaluation of total plasma antihydroxyl and antiperferryl activity is described. This method was applied to horse plasma obtained during halothane anaesthesia. In horses suffering from ... [more ▼]

A method for the evaluation of total plasma antihydroxyl and antiperferryl activity is described. This method was applied to horse plasma obtained during halothane anaesthesia. In horses suffering from postanaesthetic myositis, a significant decrease in the antiperferryl activity was observed during anaesthesia particularly when the muscular compression produced by the weight of the horse was released. In the affected muscles, strong oxidants could therefore be generated during the reperfusion of the ischaemic muscles and might initiate membrane lipid peroxidation. This phenomenon could possibly explain the muscular damage observed in equine postanaesthetic myositis. [less ▲]

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See detailEquine postanaesthetic myositis: thromboxanes, prostacyclin and prostaglandin E2 production
Serteyn, Didier ULg; Deby, Ginette ULg; Pincemail, Joël ULg et al

in Veterinary Research Communications (1988), 12

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See detailEquine pulmonary and systemic haemodynamic responses to endothelin-1 and a selective ETA receptor antagonist
Benamou, A. E.; Marlin, D. J.; Lekeux, Pierre ULg

in Equine Veterinary Journal (2001), 33(4), 337-44

Based on previous in vitro studies, we hypothesised that endothelin (ET) would induce vasoconstriction in the pulmonary circululation of the horse and that this action would be mediated via ET(A ... [more ▼]

Based on previous in vitro studies, we hypothesised that endothelin (ET) would induce vasoconstriction in the pulmonary circululation of the horse and that this action would be mediated via ET(A) receptors. Pulmonary and systemic haemodynamic responses to endothelin-1 (ET-1), a potent vasoactive endogenous peptide, were investigated in 6 conscious, nonsedated horses at rest. Bolus i.v. injections of exogenous ET-1 (0.1, 0.2 and 0.4 microg/kg bwt) caused significant increases in pulmonary (PAP) and carotid (CAP) artery pressures, with peak increases of 79% and 51% for mean PAP and CAP, respectively. The effect of ET-1 on PAP and CAP was rapid and transient for PAP (-10 min) but prolonged for CAP (up to 60 min). ET-1 significantly decreased cardiac output by up to 35% and significantly increased systemic vascular resistance (SVR) by up to 104%. Pulmonary vascular resistance (PVR) showed a trend (P>0.05) to increase with 0.2 and 0.4 microg/kg bwt ET-1. Infusion of a selective ET(A) receptor antagonist (TBC11251) completely inhibited the responses to a subsequent bolus of 0.2 microg/kg bwt ET-1. We conclude that exogenous ET-1 exerts a potent vasoconstrictive action on the pulmonary and systemic circulations of the horse. These effects appear to be mediated largely through ET(A) receptors in both circulations. Endothelin may play a role in hypertensive conditions in the horse. [less ▲]

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See detailEquine respiratory diseases
Lekeux, Pierre ULg

Book published by International Veterinary Information Service - Last Updated 2008 (2008)

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See detailEQUINE SEMEN FREEZING: STATE OF THE ART AND PERSPECTIVES.
Ponthier, Jérôme ULg

Conference (2011, November 19)

Depuis vingt ans, la congélation du sperme a permis de diffuser la génétique des étalons. Les congélations effectuées en dehors des saisons sportives permettent d’inséminer de nombreuses juments pendant ... [more ▼]

Depuis vingt ans, la congélation du sperme a permis de diffuser la génétique des étalons. Les congélations effectuées en dehors des saisons sportives permettent d’inséminer de nombreuses juments pendant que les étalons concourent dans des lieux éloignés. Les éleveurs ont en permanence accès à des doses d’insémination sans risques sanitaires. Cependant, malgré une bonne qualité de sperme frais, 20% des éjaculats équins ne supportent pas la congélation (Vidament, 2005). Le but de cette présentation est de revoir la physiologie du spermatozoïde et d’en discuter les implications dans le contexte de la congélation. Les améliorations des techniques de congélation présentes ou à venir seront aussi évoquées. [less ▲]

Detailed reference viewed: 49 (8 ULg)