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See detailEscherichia coli fusion carrier proteins act as solubilizing agents for recombinant uncoupling protein 1 through interactions with GroEL.
Douette, P.; Navet, R.; Gerkens, P. et al

in Biochemical and Biophysical Research Communications (2005), 333

Fusing recombinant proteins to highly soluble partners is frequently used to prevent aggregation of recombinant proteins in Escherichia coli. Moreover, co-overexpression of prokaryotic chaperones can ... [more ▼]

Fusing recombinant proteins to highly soluble partners is frequently used to prevent aggregation of recombinant proteins in Escherichia coli. Moreover, co-overexpression of prokaryotic chaperones can increase the amount of properly folded recombinant proteins. To understand the solubility enhancement of fusion proteins, we designed two recombinant proteins composed of uncoupling protein 1 (UCP1), a mitochondrial membrane protein, in fusion with MBP or NusA. We were able to express soluble forms of MBP-UCP1 and NusA-UCP1 despite the high hydrophobicity of UCP1. Furthermore, the yield of soluble fusion proteins depended on co-overexpression of GroEL that catalyzes folding of polypeptides. MBP-UCP1 was expressed in the form of a non-covalent complex with GroEL. MBP-UCP1/GroEL was purified and characterized by dynamic light scattering, gel filtration, and electron microscopy. Our findings suggest that MBP and NusA act as solubilizing agents by forcing the recombinant protein to pass through the bacterial chaperone pathway in the context of fusion protein. [less ▲]

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See detailEscherichia coli in domestic mammals and birds.
Mainil, Jacques ULg; Fairbrother, John

in Morabito, Stefano (Ed.) Pathogenic Escherichia coli : Molecular and cellular microbiology (2014)

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See detailEscherichia coli producing CNF1 and CNF2 cytotoxins in animals with different disorders
Pohl, P.; Oswald, E.; Van Muylem, K. et al

in Veterinary Research (1993), 24

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See detailLes Escherichia coli producteurs de shigatoxines dans les toxi-infections d'origine alimentaire.
Chahed, A.; China, B.; Daube, Georges ULg

in Annales de Médecine Vétérinaire (2007), 151

Certains Escherichia coli producteurs de Shigatoxines (STEC) sont responsables de toxi-infections d’origine alimentaire qui se traduisent par des diarrhées mais aussi par des syndromes plus graves pour ... [more ▼]

Certains Escherichia coli producteurs de Shigatoxines (STEC) sont responsables de toxi-infections d’origine alimentaire qui se traduisent par des diarrhées mais aussi par des syndromes plus graves pour l’homme comme le syndrome hémolytique urémique pouvant provoquer la mort. Il s’agit d’agents zoonotiques dont le réservoir principal est le bovin et les autres ruminants. Les principaux modes de transmission des infections à STEC à l’homme sont la consommation d’aliments contaminés (viande de boeuf peu cuite, produits laitiers non pasteurisés), la transmission de personne à personne, l’ingestion d’eau contaminée et le contact avec des animaux (notamment les bovins) et leur environnement. Les facteurs de virulence des Escherichia coli producteurs de Shigatoxines sont principalement les protéines codées par un îlot de pathogénicité, « Locus of Enterocyte Effacement », impliquées dans la formation de la lésion d’attachement et d’effacement et de la diarrhée et les toxines de Shiga codées par des bactériophages et impliquées dans les syndromes extraintestinaux. La souche de STEC du sérotype O157:H7 est responsable d’épidémies dans le monde causant des milliers de malades et des dizaines de morts. De nombreuses méthodes de diagnostic ont été développées pour identifier ce pathogène à partir des aliments. Elles regroupent des méthodes de bactériologie classique, des méthodes immunologiques et des méthodes moléculaires. Des mesures d’hygiène sont particulièrement importantes pour éviter la contamination des animaux à la ferme et celle de la viande à l’abattoir. Enfin, des modèles d’évaluation du risque ont été développés notamment afin de modéliser le comportement des STEC dans l’aliment. [less ▲]

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See detailEscherichia coli productrices de la toxine cytotoxique nécrosante de type 1 (CNF1) isolées à partir de processus pathologiques chez des chats et des chiens
Pohl, P.; Mainil, Jacques ULg; Devriese, L. et al

in Annales de Médecine Vétérinaire (1993), 137

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See detailEscherichia coli virulence factors
Mainil, Jacques ULg

in Veterinary Immunology and Immunopathology (2013), 152

Escherichia coli was described in 1885 by a German pediatrician, Theodor Escherich, in the faeces of a child suffering diarrhoea. In 1893, a Danish veterinarian postulated that the E. coli species ... [more ▼]

Escherichia coli was described in 1885 by a German pediatrician, Theodor Escherich, in the faeces of a child suffering diarrhoea. In 1893, a Danish veterinarian postulated that the E. coli species comprises different strains, some being pathogens, others not. Today the E. coli species is subdivided into several pathogenic strains causing different intestinal, urinary tract or internal infections and pathologies, in animal species and in humans. Since this congress topic is the interaction between E. coli and the mucosal immune system, the purpose of this manuscript is to present different classes of adhesins (fimbrial adhesins, afimbrial adhesins and outer membrane proteins), the type 3 secretion system, and some toxins (oligopeptide, AB, and RTX pore-forming toxins) produced by E. coli, that can directly interact with the epithelial cells of the intestinal, respiratory and urinary tracts. [less ▲]

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See detailLes esclaves malfaiteurs dans l'Egypte romaine
Straus, Jean ULg

in Tallet, Gaëlle; Zivie-Coche, Christiane (Eds.) Le Myrte et la Rose. Mélanges offerts à Françoise Dunand par ses élèves, collègues et amis (2014)

Crimes commited by slaves in Roman Egypt

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See detailEscouloubre Piano Key weir: prototype versus scale models
Erpicum, Sébastien ULg; Silvestri, Aurélien ULg; Dewals, Benjamin ULg et al

in Erpicum, Sébastien; Laugier, Frédéric; Pfister, Michael (Eds.) et al Labyrinth and Piano Key Weirs II - PKW 2013 (2013)

A 1.77 m high piano key weir in operation on the Escouloubre balance basin enabled the observation of real size flows for discharges ranging from 1.2 to 10 m³/s, corresponding to upstream heads ranging ... [more ▼]

A 1.77 m high piano key weir in operation on the Escouloubre balance basin enabled the observation of real size flows for discharges ranging from 1.2 to 10 m³/s, corresponding to upstream heads ranging from 11 to 48 cm. These observations are depicted and compared to the results gained on three physical models of the weir, built and operated considering a Froude similitude and geometric scale factors of, respectively, 1:7, 1:15 and 1:25. The study highlights the scale effects which may affect piano key weirs scale model studies and provides criteria to limit them. In particular, the results analysis shows that the scale models succeed in representing the flow conditions on the weir when the model upstream head is higher than 60 mm, and succeed in predicting the weir discharge capacity when the model upstream head is higher than 30 mm. [less ▲]

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See detailEscourgeon et Orge d'hiver
Herman, Jean-Luc; Bodson, Bernard ULg; Monfort, Bruno et al

in Livre Blanc: Céréales - Gembloux - Informations avant les semis (1997, September 11)

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See detailEscourgeon et Orge d'hiver fourragère
Bodson, Bernard ULg; Herman, Jean-Luc; Monfort, Bruno et al

in Fumure et protection phytosanitaire des céréales: Informations avant les semis des céréales (1998, September 10)

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See detailEscourgeon et Orge d'hiver fourragers
Bodson, Bernard ULg; Monfort, Bruno; Herman, Jean-Luc et al

in Livre Blanc: Céréales - Gembloux - Informations avant les semis (2002, September 12)

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See detailEscourgeon et Orge d'hiver fourragers
Monfort, Bruno; Herman, Jean-Luc; Bodson, Bernard ULg et al

in Livre Blanc: Céréales - Gembloux - Informations avant les semis (2004, September 09)

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See detailEscourgeon et Orge d'hiver fourragers
Herman, Jean-Luc; Monfort, Bruno; Couvreur, Luc et al

in Livre Blanc: Céréales - Gembloux - Informations avant les semis (2005, September 08)

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See detailEscourgeon et Orge d'hiver fourragers
Monfort, Bruno; Herman, Jean-Luc; Couvreur, Luc et al

in Livre Blanc: Céréales - Gembloux - Informations avant les semis (2006, September 07)

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See detailEscourgeon et Orge d'hiver fourragers
Herman, Jean-Luc; Bodson, Bernard ULg; Monfort, Bruno et al

in Livre Blanc: Céréales - Gembloux - Informations avant les semis (1999, September 09)

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