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See detailDevelopment of an original approach to evaluate effects of surfactants, biomass and pollutants on the scaling-up of a two-phase partitioning bioreactor
Aldric, Jean-Marc ULg; Gillet, Sébastien ULg; Delvigne, Frank ULg et al

in Journal of Chemical Technology & Biotechnology (2010), 84

BACKGROUND: Two-phase partitioning bioreactors (TPPBs) are considered as a new technology for xenobiotic degradation in gaseous effluents. However, there is still a need for more knowledge on how to ... [more ▼]

BACKGROUND: Two-phase partitioning bioreactors (TPPBs) are considered as a new technology for xenobiotic degradation in gaseous effluents. However, there is still a need for more knowledge on how to design and scale-up TPPBs. The partitioning of the two phases remains a misunderstood way of research. In particular, the impact of pollutant (isopropylbenzene), biomass and surfactant extract needs to be better evaluated. RESULTS:. An adaptated scale-down apparatus has been developed in order to quantify the speed of phase partitioning (SPP) into a plug flow section. Firstly, it was shown that isopropylbenzene (IPB) doesn’t destabilize more significantly the system. Secondly, respectively 0.5 g.L-1 and 0.05 g.L-1 of biomass and surfactant extract, separately or in mixture, were sufficient to ensure the stability of the two-phase system. Finally, a 100 m3 limit of scaling-up was suggested on the basis of the circulation time comparison. CONCLUSION: The scaling-up of an aqueous/silicone-oil TPPB was found to be definitely conceivable when the presence of biotic compounds were considered. However, further considerations are needed to verify our assumptions, in particular by taking into account the velocity field pattern in full-scale bioreactors and reproduce it in lab-scale apparatus. [less ▲]

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See detailDevelopment of an original approach to evaluate effects of surfactants, biomass and pollutants on the scaling-up of a two-phase partitioning bioreactor
Aldric, Jean-Marc ULg

(2009)

BACKGROUND: Two-phase partitioning bioreactors (TPPBs) are considered as a new technology for xenobiotic degradation in gaseous effluents. However, there is still a need for more knowledge on how to ... [more ▼]

BACKGROUND: Two-phase partitioning bioreactors (TPPBs) are considered as a new technology for xenobiotic degradation in gaseous effluents. However, there is still a need for more knowledge on how to design and scale-up TPPBs. The partitioning of the two phases remains a misunderstood way of research. In particular, the impact of pollutant (isopropylbenzene), biomass and surfactant extract needs to be better evaluated. RESULTS:. An adaptated scale-down apparatus has been developed in order to quantify the speed of phase partitioning (SPP) into a plug flow section. Firstly, it was shown that isopropylbenzene (IPB) doesn’t destabilize more significantly the system. Secondly, respectively 0.5 g.L-1 and 0.05 g.L-1 of biomass and surfactant extract, separately or in mixture, were sufficient to ensure the stability of the two-phase system. Finally, a 100 m3 limit of scaling-up was suggested on the basis of the circulation time comparison. CONCLUSION: The scaling-up of an aqueous/silicone-oil TPPB was found to be definitely conceivable when the presence of biotic compounds were considered. However, further considerations are needed to verify our assumptions, in particular by taking into account the velocity field pattern in full-scale bioreactors and reproduce it in lab-scale apparatus. [less ▲]

Detailed reference viewed: 52 (6 ULg)
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See detailDevelopment of an original intra-uterine implant allowed specific release kinetics of several drugs
Nizet, Dominique; Zalfen, Alina; Collard, Laurence ULg et al

Poster (2007)

Detailed reference viewed: 28 (1 ULg)
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See detailDevelopment of an RMS model based on colored object-oriented Petri nets with changeable structures
Zhang, Linda; Ittoo, Ashwin ULg

in IEEE Proceedings of the 16th International Conference on Industrial Engineering and Engineering Management (2009)

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See detailDevelopment of an urban typology to assess residential environmental performance at the city scale
Wallemacq, Véronique; Marique, Anne-Françoise ULg; Reiter, Sigrid ULg

in Evrard Arnaud, Bodard Magali (Ed.) Proceedings of International Conference PLEA 2011 : Architecture & Sustainable Development (2011, July)

In this research, a typology of urban blocks is drawn up for the urban area of Liege. This typology of urban blocks is organized into a set of themes according to various environmental parameters. This ... [more ▼]

In this research, a typology of urban blocks is drawn up for the urban area of Liege. This typology of urban blocks is organized into a set of themes according to various environmental parameters. This paper presents the energy part of this typology on the residential building stock of Liege, which includes four topics: residential buildings energy consumption; transport energy consumption of residents; development potentialities of public transport and development potentialities of energy networks. The proposed typology was elaborated through the use of GIS tools combined with a statistical treatment of several specific criteria at the urban block scale. For each class of this typology, a representative block is selected for further energy simulations in order to model residential energy use related to buildings, transport and energy networks at the city scale. The methodology developed in this paper is adapted to urban, suburban and rural zones. It can thus be adapted and/or reproduced on many other territories in Belgium but also in Europe or even further. [less ▲]

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See detailDEVELOPMENT OF ANALYTICAL TOOLS FOR THE DETERMINATION OF MARKERS OF LIPID ALTERATION IN FOOD
Douny, Caroline ULg

Doctoral thesis (2017)

It is now well-known that n-3 polyunsaturated fatty acids (omega-3 PUFA) have beneficial effects on health but are also rapidly oxidized in potentially toxic compounds, which means that healthy food ... [more ▼]

It is now well-known that n-3 polyunsaturated fatty acids (omega-3 PUFA) have beneficial effects on health but are also rapidly oxidized in potentially toxic compounds, which means that healthy food products, rich in n-3 PUFA, can become toxic for the consumer if not stored or processed appropriately. The main objective of this work was to develop new analytical tools to study markers of lipids alteration in food, in order to allow an adequate monitoring of possible toxic compounds in n-3 PUFA rich food products. The first chapter of this PhD thesis is a literature review about fatty acids, their oxidation and the different oxidation products generated. The different analytical techniques used to study the oxidative stability of polyunsaturated fatty acids and their degradation products in food are also described. Chapter 2 describes the development of a gas chromatography coupled to mass spectrometry (GC-MS) method to analyze the fatty acids in food. The method was applied to eggs and pork (both standard and n-3 enriched) submitted to various cooking processes and storage conditions. This study confirmed that the fat of standard eggs or pork from the Belgian market cannot be considered as a source ofn-3 PUFA.. Indeed, in this work, we measured no n-3 PUFA in meat or only around 2 % for eggs, while the fat of both enriched eggs and pork contained about 10 % of n-3 fatty acids. After storage or cooking without culinary fat no change of the fatty acid profile was observed, in standard eggs or pork. We made the same observation for n-3 enriched products, except for some cooking processes (pan-frying and oven cooking), which induced a slight but significant loss of n-3 fatty acids in n-3 enriched eggs or pork. The fairly stable behavior of the fatty acids after various cooking and storage experiments indicates that the fatty acid profile is not a good marker of lipid alteration. As a consequence, this work was oriented to the development of analytical tools to evaluate the presence of toxic aldehydes in food and feed as secondary oxidation products coming from polyunsaturated fatty acids. Chapter 3 is dedicated to the study of the oxidative stability of linseed oil, an oil rich in n-3 fatty acids. Section 3.1 describes the development of a liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method to evaluate the aldehyde content in linseed oil. After optimisation of the extraction, derivation, detection and quantification steps, the method was validated according to guidelines from the European legislation as well as the recommendations of the AFNOR protocol NF V 03-110 (AFNOR, 2010). The evaluated parameters included specificity/selectivity, recovery, precision, accuracy, uncertainty, limits of detection and quantification, determined using the concept of accuracy profiles. When the method was applied to first pressure linseed oil stored for several days at 60 °C according to the Schaal oven test, it was shown that 4-hydroxy-2E-hexenal (4-HHE) was the most produced aldehyde. In Section 3.2, the different patterns of oxidation of linseed oil in real-time and accelerated aging assays were investigated, in order to verify the hypothesis commonly accepted for vegetal oil that one day aging at 60 °C is equivalent to one month real time aging at room temperature. Oxidation was evaluated with the peroxide value and para-anisidine value, as well as the content in conjugated dienes and aldehydes. All four indicators of oxidation showed very different kinetic behaviours at 20 and 60 °C, showing that the hypothesis (1 day at 60 °C = 1 month at 20 °C) is not valid for linseed oil. Chapter 4 describes the adaptation and the validation of a LC-MS/MS method to determine aldehydes in animal feed samples. Indeed, as animal feed is often enriched with linseed to increase the human intake of n-3 fatty acids through the consumption of food from animal origin, the study of the oxidative stability of n-3 enriched animal feed is also of interest. To achieve that goal, the LC-MS/MS method previously developed for linseed oil was adapted to include more aldehydes and was validated using the same guidelines as in section 3.1. Finally, chapter 5 presents the discussion and conclusions about the developed methods and the results obtained as well as their comparison with literature. Future prospects to improve the evaluation of and the exposure to the markers of lipid alteration in food are proposed. [less ▲]

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See detailDevelopment of anti-HPV lipoplexes for the treatment of cervical cancer
Lechanteur, Anna ULg; Furst, Tania ULg; Evrard, Brigitte ULg et al

Conference (2013, October 17)

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See detailDevelopment of anti-HPV lipoplexes for the treatment of cervical cancer
Lechanteur, Anna ULg; Furst, Tania ULg; Evrard, Brigitte ULg et al

Conference (2013, December 03)

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See detailDevelopment of aptamer-based radiopharmaceuticals for targeted cancer imaging and therapy
Gijs, Marlies ULg; Aerts, An; Impens, Nathalie et al

Poster (2013, March 04)

This SELEX experiment, for the selection of HER2 targeting aptamers, resulted in 26 selected RNA aptamers for further individual evaluation. Up till now, we were able to identify two aptamers that show ... [more ▼]

This SELEX experiment, for the selection of HER2 targeting aptamers, resulted in 26 selected RNA aptamers for further individual evaluation. Up till now, we were able to identify two aptamers that show binding to HER2 overexpressing SK-OV-3 cancer cells. In addition, we were able to generate silenced SK-OV-3 cells with minimal remaining HER2 levels, which will be used to obtain more information regarding the binding specificity of the selected aptamers. [less ▲]

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See detailDevelopment of aquaculture in North Vietnam: influence on the living standards and gender division of family labor
Caulier, Henri; Tran Thi Thap, Hieu; Tran Thi Nang, Thu et al

Poster (2016)

During previous years, along with the overall development of Vietnam, the fisheries sector has made great progress in terms of output and has remained as a very important sector of Vietnam’s economy. The ... [more ▼]

During previous years, along with the overall development of Vietnam, the fisheries sector has made great progress in terms of output and has remained as a very important sector of Vietnam’s economy. The average growth rate of this sector has been 7.9% per year –contributing greatly to the industrialization and modernization of the agriculture and rural sectors of the country. Since 2006, Vietnam has been globally ranked among the top 10 leading exporting countries in fisheries. To cope with these new circumstances, the aquaculture sector must grow while reducing emissions and pollution. Moreover, the value they generate should be distributed fairly, in order to improve the living conditions of producers, while the risks must be shared among all stakeholders. In this context, il will be also appropriate to conduct specific research including gender analysis of aquaculture value chain. The main objective of this paper is to present the main results of a field research conducted in the northern Vietnam and related to a specific fresh water aquaculture production system. This study was conducted to (1) describe and understand the division of family labor in aquaculture and (2) make a diagnose about economic situation of the households in aquaculture. [less ▲]

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See detailDevelopment of Astrocytes in the Sheep Cerebellum
Salouci, Moustafa ULg; Antoine, Nadine; Shikh Al Sook, Mohamad Khir ULg et al

Scientific conference (2013, October 11)

Detailed reference viewed: 92 (57 ULg)
See detailDevelopment of atherosclerosis for the vascular surgeon.
Defraigne, Jean-Olivier ULg

in Liapis, C. D. (Ed.) European Manual of Medicine (2007)

Detailed reference viewed: 10 (1 ULg)