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See detailDevelopment Of Real-Time Pcr For The Rapid Detection Of Episomal Banana Streak Virus (Bsv)
Delanoy, Martine; Salmon, Marc ULg; Kummert, Jean et al

in Plant Disease (2003), 87(1), 33-38

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See detailDevelopment Of Real-Time Pcr Using Minor Groove Binding Probe To Monitor The Biological Control Agent Candida Oleophila (Strain O)
Massart, Sébastien ULg; De Clercq, Deborah; Dickburt, Catherine et al

in Journal of Microbiological Methods (2005), 60(1), 73-82

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See detailDevelopment Of Real-Time Rt-Pcr Assay For Detection Of Prunus Necrotic Ringspot Virus In Fruit Trees
Marbot, Sophie; Vendrame, Marina; Huwaert, Astrid et al

in Plant Disease (2003), 87(11), 1344-1348

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See detailDevelopment of recombinant stable house dust mite allergen Der p 3 molecules for component resolved diagnosis and specific immunotherapy
Bouaziz, Ahlem ULg; Galleni, Moreno ULg; Louis, Renaud ULg

in Clinical and Experimental Allergy (2015), 45

Abstract BACKGROUND: The allergen Der p 3 is underrepresented in house dust mite (HDM) extracts probably due to autolysis. Recombinant stable molecule of the allergen is thus needed to improve the ... [more ▼]

Abstract BACKGROUND: The allergen Der p 3 is underrepresented in house dust mite (HDM) extracts probably due to autolysis. Recombinant stable molecule of the allergen is thus needed to improve the diagnosis of allergy and the safety and efficacy of immunotherapy. OBJECTIVE: The current study reports the immunological characterization of two recombinant molecules of the HDM allergen Der p 3 as useful tools for diagnosis and immunotherapy. METHODS: Recombinant mature (rDer p 3) and immature (proDer p 3) Der p 3 and their corresponding S196A mutants were produced in Pichia pastoris and purified. The stability, IgE-binding capacity and allergenicity of the different proteins were analysed and compared with those of the major mite allergen Der p 1 used as a reference. Additionally, the immunogenicity of the different allergens was evaluated in a murine model of Der p 3 sensitization. RESULTS: Compared to the IgE reactivity to recombinant and natural Der p 3 (nDer p 3), the mean IgE binding of patient's sera to rDer p 3-S196A (50%) was higher. The poorly binding to nDer p 3 or rDer p 3 was due to autolysis of the allergen. Contrary to Der p 3, proDer p 3 displayed very weak IgE reactivity, as measured by sandwich ELISA and competitive inhibition, rat basophil leukaemia degranulation and human basophil activation assays. Moreover, proDer p 3 induced a TH 1-biased immune response that prevented allergic response in mice but retained Der p 3-specific T-cell reactivity. CONCLUSION: rDer p 3-S196A should be used for the diagnosis of HDM allergy elicited by Der p 3, and proDer p 3 may represent a hypoallergen of Der p 3. [less ▲]

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See detailDevelopment of recombinant stable house dust mite allergen Der p 3 molecules for component-resolved diagnosis and specific immunotherapy.
Bouaziz, Ahlem ULg; Walgraffe, David; Bouillot, Celine et al

in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (2014)

BACKGROUND: The allergen Der p 3 is underrepresented in house dust mite (HDM) extracts probably due to autolysis. Recombinant stable molecule of the allergen is thus needed to improve the diagnosis of ... [more ▼]

BACKGROUND: The allergen Der p 3 is underrepresented in house dust mite (HDM) extracts probably due to autolysis. Recombinant stable molecule of the allergen is thus needed to improve the diagnosis of allergy and the safety and efficacy of immunotherapy. OBJECTIVE: The current study reports the immunological characterization of two recombinant molecules of the HDM allergen Der p 3 as useful tools for diagnosis and immunotherapy. METHODS: Recombinant mature (rDer p 3) and immature (proDer p 3) Der p 3 and their corresponding S196A mutants were produced in Pichia pastoris and purified. The stability, IgE-binding capacity and allergenicity of the different proteins were analyzed and compared with those of the major mite allergen Der p 1 used as a reference. Additionally, the immunogenicity of the different allergens was evaluated in a murine model of Der p 3 sensitization. RESULTS: Compared to the IgE reactivity to recombinant and natural Der p 3 (nDer p 3), the mean IgE binding of patient's sera to rDer p 3-S196A (50 %) was higher. The poorly binding to nDer p 3 or rDer p 3 was due to autolysis of the allergen. Contrary to Der p 3, proDer p 3 displayed very weak IgE reactivity, as measured by sandwich ELISA and competitive inhibition, RBL degranulation and human basophil activation assays. Moreover, proDer p 3 induced a TH 1-biased immune response that prevented an allergic response in mice but retained Der p 3-specific T cell reactivity. CONCLUSION: rDer p 3-S196A should be used for the diagnosis of HDM allergy elicited by Der p 3, and proDer p 3 may represent a hypoallergen of Der p 3. This article is protected by copyright. All rights reserved. [less ▲]

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See detailDevelopment of response models for optimising HPLC methods
Dewé, Walthère ULg; Marini Djang'Eing'A, Roland ULg; Chiap, Patrice ULg et al

in Chemometrics and Intelligent Laboratory Systems (2004), 74(2), 263-268

For optimizing a liquid chromatographic method, direct modeling some responses like minimum resolution is sometimes difficult to perform. By modeling the retention time at apex and the times associated to ... [more ▼]

For optimizing a liquid chromatographic method, direct modeling some responses like minimum resolution is sometimes difficult to perform. By modeling the retention time at apex and the times associated to 13.4% of the peak height, it is possible to obtain estimations of these responses using an adequate cascade of equations, these equations being the definitions of these responses. As a multi-criteria decision has to be taken in most of these optimizations, a Derringer's function can be defined for each response of interest and a overall desirability, on which the final is taken, can be derived. In addition, the chromatogram in any experimental point can be predicted. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailDevelopment of rice vermicelli processing in Dinh Ke and Thu Duong traditional craft villages, Bac Giang province
Le Thi Long, Vy; Lebailly, Philippe ULg

in Journal of Economics & Development (2016), 229(II (07/2016)), 60-66

This study aims at analyzing the development processes of rice vermicelli in Dinh Ke and Thu Duong villages of Bac Giang province. The authors conducted a survey with 66 households involving rice ... [more ▼]

This study aims at analyzing the development processes of rice vermicelli in Dinh Ke and Thu Duong villages of Bac Giang province. The authors conducted a survey with 66 households involving rice vermicelli production and 51 households who did not involve. In combination with in-depth interviews with representative leaders of "Cooperative of production and trading of rice vermicelli in Dinh Ke" and "Association of production and consumption of Chu vermicelli in Luc Ngan district", the data indicated that not only did the development of traditional craft villages bring high income for farm households but they also helped to conserve Vietnam cuisine culture. In order to develop traditional craft villages, it is necessary to re-establish linkages from a production stage to marketing of products, to ensure adequate facilities for drying products, and to impose strong sanction to deal with illegal trading. [less ▲]

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See detailDevelopment of riveting technology through an analysis of Belgian patents (1830-1940)
Collette, Quentin; Wouters, Ine; de Favereau, Corentin et al

in Jasienko, Jerzy (Ed.) Proceedings of the International Conference on Structural Analysis of Historical Constructions (SAHC 2012) (2012)

The development of riveting technology in iron and steel structures in the 19th and 20th centuries were highly promoted by patents. Although riveting was widely used in buildings and civil engineering ... [more ▼]

The development of riveting technology in iron and steel structures in the 19th and 20th centuries were highly promoted by patents. Although riveting was widely used in buildings and civil engineering structures, little information about the development of this technology – how rivets were manufactured, various installation techniques – is available in literature. This paper discusses a database of patents related to riveting technology that was created to better understand the innovations in riveted connections and their technological evolution. The database includes patents relating to rivets registered in Belgium between 1830 and 1940. Belgian patents are a good proxy for inventiveness in riveting technology in this period, in industrialized countries generally, not only Belgium. As one of the most industrialized countries, Belgium attracted inventors from many countries seeking to register their inventions: foreign inventions represented approximately 75% of all patents. Breakthroughs by important Belgian, French, British, and American inventors/companies are represented in this source. A methodology based on both quantitative and qualitative analyses was implemented. The quantitative analysis dealt with the entire database and explored several key parameters, for example, the subject of the patent and the patenting organization/person. The database also permitted qualitative analyses; specifically, it helped enlarge our understanding of pioneering inventors and their inventions, in the important categories of rivet manufacturing (1855-1870) and installation techniques (1895-1910). As background for the conservation and renovation of historic iron and steel structures, it is vital to have an understanding of the know-how that was available over time. This kind of information is revealed in the patent record [less ▲]

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See detailDevelopment of routine dtection tests using PCR for certification
Chandelier, Anne; Cognet, Stephane; Marinho, Véra Lucia et al

in Med. Fac. Landbouw. Univ. Gent, 63/4b, 1998 (1998)

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See detailDevelopment of routine duplex RT-PCR tests for certification of fruit tree multiplication material
Massart, Sébastien ULg; Roussel, S.; Kummert, J. et al

Conference (2006)

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See detailDevelopment of routine duplex RT-PCR tests for certification of fruit tree multiplication material
Massart, Sébastien ULg; Roussel, S.; Kummert, J. et al

in Acta Horticulturae (2008), 781

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See detailDevelopment of routine RT-PCR ELOSA tests for fruit tree certification.
Kummert, Jean; Vendrame, Marina; Lepoivre, Philippe ULg et al

in Acta Horticulturae (2001), (550 (Vol. 1)),

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See detailDevelopment of routine RT-PCR tests for certification of fruit tree multiplication material.
Kummert, Jean; Vendrame, Marina; Steyer, Stephane et al

in Bulletin OEPP (2000), 30(3-4), 441-448

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See detailDevelopment of RT-PCR assays using fluorogenic 3 minor groove binder DNA probes for detection of fruit tree viruses.
Marbot, Sophie; Kummert, Jean; Vendrame, Marina et al

in Acta Horticulturae (2004), (657), 547-551

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See detailDevelopment of RT-PCR assays using fluorogenic 3' minor groove binder DNA probes for detection of fruit trees viruses
Roussel, S.; Kummert, J.; Salmon, M. et al

Conference (2004)

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See detailDevelopment of RT-PCR assays using fluorogenic-3 minor groove binder DNA probes for detection of fruit tree viruses.
Roussel, S.; Kummert, J.; Dutrecq, O. et al

in Acta Horticulturae (2004), 657(1),

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See detailDevelopment of RT-PCR tests for the routine detection of latent and ILAR viruses in fruit trees.
Marbot, Sophie; Kummert, Jean; Salmon, Marc ULg et al

in Plant Protection Science (2002), 38(Special 1),

Detailed reference viewed: 27 (3 ULg)