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See detailENZYMATIC INTERESTERIFICATION OF PALM OIL AND FRACTIONS
Gibon, Véronique; Danthine, Sabine ULg; De Clercq, Nathalie et al

Conference (2009, May)

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See detailEnzymatic interesterification of palm oil and fractions: monitoring the degree of interesterification using different methods.
De Clercq, Nathalie; Danthine, Sabine ULg; Nguyen, Mai et al

Poster (2011, September)

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See detailEnzymatic Interesterification of Palm Oil and Fractions:Monitoring the Degree of Interesterification using Different Methods
De Clercq, N.; Danthine, Sabine ULg; Tuyet Nguyen, M. et al

in Journal of the American Oil Chemists' Society (2012), 89

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See detailEnzymatic Interesterificationof Palm oil and Fractions: A Calorimetric Study
Danthine, Sabine ULg; De Clercq, Nathalie; Lefebure, Emilie ULg et al

Poster (2011, September)

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See detailEnzymatic method for rapid and sensitive determination of β-lactam antibiotics
Frère, Jean-Marie ULg; Klein, Daniel; Ghuysen, Jean-Marie ULg

in Antimicrobial Agents and Chemotherapy (1980), 18(4), 506-510

A rapid and sensitive procedure for the estimation of beta-lactam antibiotics is described which makes use of the ability of these antibiotics to inactivate the R39 DD-carboxypeptidase. Depending on the ... [more ▼]

A rapid and sensitive procedure for the estimation of beta-lactam antibiotics is described which makes use of the ability of these antibiotics to inactivate the R39 DD-carboxypeptidase. Depending on the values of the kinetic parameters which govern the reaction, the antibiotics fall into two groups. The lower limit for the quantitative estimation of the antibiotics of groups I and II is about 5 and 50 pmol/ml, respectively. The procedure has been adopted to biological fluids such as human sera and cows' milk. [less ▲]

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See detailEnzymatic modifications of sugar in supercritical carbon dioxide
Favrelle, Audrey ULg; Brognaux, Alison ULg; Debuigne, Antoine ULg et al

Poster (2009, July 07)

Carbohydrates esters are non-ionic surfactants that have a wide range of commercial applications in cosmetic, food and pharmaceutical industry. They are produced from renewable and inexpensive raw ... [more ▼]

Carbohydrates esters are non-ionic surfactants that have a wide range of commercial applications in cosmetic, food and pharmaceutical industry. They are produced from renewable and inexpensive raw materials, are bio-degradable and non-toxic. Chemical synthesis of sugar esters is generally performed at a high temperature in the presence of an alkaline catalyst lead-ing to a mixture of products. In this respect, the corresponding enzyme-catalyzed processes in non-conventional media are more selective. For this purpose, lipases are the most useful enzymes. Moreover, supercritical carbon dioxide (SC-CO2) constitutes an interesting alternative to the organic solvents used in the domain as it is considered to be environmentally frien-dlier and safer. For example, its use reduces the contamination of the final products with residual solvents. This property is particularly valued in food, cosmetic and pharmaceutical industry. Our work consists to carry out lipase catalyzed sugar modifications in SC-CO2 and to compare the results with those obtained in organic solvents. The effect of these two different media on the enzyme stability and the yield will be described here. Moreover, the impact of various factors such as pressure, temperature, enzyme form (free or immobilized), use of co-solvent, on the course of the sugar esterification will be discussed. [less ▲]

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See detailEnzymatic process development for the extraction of ferulic acid from wheat bran
Giet, Jean-Michel ULg; Roiseux, O.; Blecker, Christophe ULg

Poster (2010, February 16)

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See detailEnzymatic process development for the extraction of ferulic acid from wheat bran
Giet, Jean-Michel ULg; Blecker, Christophe ULg

Poster (2012)

The agro-industries generate each year thousands of tons of by-products, such as cereal bran or sugar beet pulps. For instance, Walloon wheat transformation industry provides annually about 200.000 tons ... [more ▼]

The agro-industries generate each year thousands of tons of by-products, such as cereal bran or sugar beet pulps. For instance, Walloon wheat transformation industry provides annually about 200.000 tons of bran. Most of those by-products are under-valorized as cattle feed. By the use of biorefinery, this biomass may constitute a renewable source for various value-added molecules like dietary fibres, proteins, antioxidants, and more. [less ▲]

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See detailEnzymatic process for the fractionation of baker's yeast cell wall (saccharomyces cerevisiae)
Borchani, Chema; Fonteyn, Fabienne; Jamin, Guilhem et al

Conference (2014, April 07)

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See detailEnzymatic process for the fractionation of baker’s yeast cell wall (Saccharomyces cerevisiae)
Borchani, Chema; Fonteyn, Fabienne; Jamin, Guilhem et al

in Food Chemistry (2014), 163

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See detailEnzymatic production of pectic oligosaccharides from polygalacturonic acid with commercial pectinase preparations
Combo, Agnan Marie Michel ULg; Aguedo, Mario ULg; Goffin, Dorothée ULg et al

in Food and Bioproducts Processing: Transactions of the Institution of Chemical Engineers, Part C (2011), 90(3), 588-596

The present study investigates the individual efficiency of six commercial pectinase preparations (Endopolygalacturonase M2, Pectinase, Viscozyme L, Pectinex Ultra SP-L, Pectinase 62L and Macer8 FJ) in ... [more ▼]

The present study investigates the individual efficiency of six commercial pectinase preparations (Endopolygalacturonase M2, Pectinase, Viscozyme L, Pectinex Ultra SP-L, Pectinase 62L and Macer8 FJ) in catalyzing the liberation of pectic oligosaccharides (POS) from polygalacturonic acid. On the basis of high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) analysis of the enzymatic hydrolysates, products release kinetics revealed a random cleavage pattern and an exo mode of cleavage for all the enzymes except for Endopolygalacturonase M2. All six enzymes generated oligoGalA with different degree of polymerization (DP); the quantitative composition of oligoGalA depended on the enzyme specificity and the time of enzymatic reaction. Endopolygalacturonase M2 was the best enzyme preparation for production of oligoGalA, with 18% (wt) of digalacturonic acid and 58% (wt) of trigalacturonic acid after 2h of reaction. Concerning galacturonic acid production, Pectinase 62L was superior to the other enzyme preparations with 47% (wt) after 1h of reaction. [less ▲]

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See detailENZYMATIC PRODUCTION OF PURIFIED ISOMALTOOLIGOSACCHARIDES PREBIOTIC PREPARATIONS AND THEIR FULL QUALITATIVE AND QUANTITATIVE CHARACTERIZATION
Goffin, Dorothée ULg

Doctoral thesis (2010)

Prebiotics are non-digestible food ingredients that beneficially affect the host health by stimulating the growth and/or activity of one or a limited number of bacteria in the colon, and moreover can have ... [more ▼]

Prebiotics are non-digestible food ingredients that beneficially affect the host health by stimulating the growth and/or activity of one or a limited number of bacteria in the colon, and moreover can have systemic effects on metabolism regulation. Recent studies have pointed out the certain links between their structure (DP, types of linkage, sequence) and the specificity and intensity of their effects on host health. Therefore, the knowledge of the exact composition of prebiotic preparations has become a crucial step in the development of new products. Amongst these prebiotics, isomaltooligosaccharides (IMO), produced enzymatically from hydrolyzed starch, are the most developed in Asia thanks to their many favourable properties for application in food industry. However, their full characterization hasn’t been achieved yet. In the present thesis, an HPAEC-PAD method has been set up in order to quantify the IMO present in syrups. Moreover, this step-forward analytical method permitted us to point out the presence of unknown IMO. Two structural determination methods were then set up and applied. PGC-LC-ESI-IT-MS2 including a chromatographic separation on a porous graphitized carbon column and producing MS2 fragment ion profiles specific to the linkage position proved to be promising but delicate for certain combination of linkage, while 1D and 2D NMR experiments give unambiguous structural determinations but need a preliminary chromatographic preparation step. Moreover, two enzymes from different families (a glucosyl-transferase and an α-glucosidase) were tested on maltose individually or in combination. The IMO kinetics of formation and mixtures obtained were compared with a view to produce IMO preparations with singular profiles. Finally, a new original method was set up to convert deleterious digestible saccharides, present in IMO preparations, into gluconic acid which presents various advantageous techno-functional properties as well as nutritional properties, in particular, prebiotic. [less ▲]

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See detailEnzymatic propyl gallate synthesis in solvent-free system: Optimization by response surface methodology
Bouaziz, Ahlem ULg; Horchani, Habib; Ben Salem, Nadia et al

in Journal of Molecular Catalysis B: Enzymatic (2010), 67

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See detailEnzymatic remediation of crude palm oil
Gibon, Véronique; Kodali, Sitharam; Maes, Jeroen et al

Poster (2013)

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See detailEnzymatic synthesis and surface active properties of novel hemifluorinated mannose esters
Favrelle, Audrey ULg; Boyère, Cédric ULg; Laurent, Pascal ULg et al

in Carbohydrate Research (2011), 346(9), 1161-1164

The lipase-catalysed esterification of sugars with hemifluorinated acid derivatives is reported for the first time. A series of mannose modified derivatives having fluorinated chains with different length ... [more ▼]

The lipase-catalysed esterification of sugars with hemifluorinated acid derivatives is reported for the first time. A series of mannose modified derivatives having fluorinated chains with different length have been prepared accordingly in moderate yield. A preliminary evaluation of the surface active properties of these hemifluorinated mannose esters revealed their ability to reduce the surface tension of water much more efficiently than their aliphatic counterparts. [less ▲]

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See detailEnzymatic synthesis and surface properties of novel rhamnolipids
Nott, Katherine ULg; Richard, Gaetan ULg; Laurent, Pascal ULg et al

in Process Biochemistry (2013), 48

New rhamnolipids were obtained via the development of a synthesis procedure consisting of two biocatalyzed steps. In the first step, naringinase was used to introduce a primary alcohol function onto ... [more ▼]

New rhamnolipids were obtained via the development of a synthesis procedure consisting of two biocatalyzed steps. In the first step, naringinase was used to introduce a primary alcohol function onto rhamnose by glycosylation of 1,3-propanediol. In the second step, immobilized lipase B from Candida antarctica catalyzed the esterification of the primary hydroxyl group with mono- and di-carboxylic fatty acids of increasing chain length (from C8 to C14). For the monoic acids, the initial rate and 24 h yield decreased with increasing chain length. For the dioic acid, the number of carbon atoms of the acid did not influence these parameters. The new rhamnolipid obtained with tetradecanoic acid showed very good surface properties. At pH 5, it had a very low critical aggregation concentration of 1.70 M and it diminished water’s surface tension to 27.6 mN/m. It was also able to form stable insoluble monolayers. On the other hand, the rhamnolipid formed with tetradecanedioic acid showed far less interesting surface properties. [less ▲]

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See detailEnzymatic synthesis of glycosylated compounds in non-conventional media
Galonde, Nadine ULg

Doctoral thesis (2013)

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See detailEnzymatic synthesis of sugar esters obtained from renewable stabilized by dairy components.
Torezan, Gabriela; Ronkart, Sébastien; Paquot, Michel ULg et al

Poster (2007, October 11)

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