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See detailThe development of knowledge regarding the characteristics of the proteose peptone fraction of milk: Techno-functional and biological properties. A review
Karamoko, Gaoussou ULg; Anihouvi, Prudent; Blecker, Christophe ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment (2013), 17(2), 373-382

The total proteose-peptone fraction (TPP) is defined as a heat-stable soluble fraction of milk, representing about 10% of the whey protein. TPP is divided into two classes according to its origin. The ... [more ▼]

The total proteose-peptone fraction (TPP) is defined as a heat-stable soluble fraction of milk, representing about 10% of the whey protein. TPP is divided into two classes according to its origin. The first class consists of proteolysis fragments of the β-casein from the N-terminal region. These are non-hydrophobic fractions, which are the highly soluble β-CN-5P (f1-105/107); β-CN-4P (f1-28) and β-CN-1P (f29-105/107) respectively called PP5 (14.3 kDa), PP8S (9.9 kDa), and PP8F (4 kDa). The second class includes the hydrophobic fractions of glycoproteins, whose major constituents are a glycoprotein LP28, the highly hydrophobic glycoprotein LP18 and a hydrophobic peptide with apparent Mr, respectively 28 kDa, 18 kDa and 11 kDa. TPP has numerous interesting characteristics such as techno-functional properties (emulsifying and foaming actions) and biological properties (lipolysis inhibition and antimicrobial activities), making TPP usable as a potential functional ingredient for industry. In addition, these functional properties are partly governed by the major components including glycoproteins, such as LP28, due to their hydrophobic nature. [less ▲]

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See detailDevelopment of lead-210 measurement in peat using polonium extraction. A procedural comparison
De Vleeschouwer, François; Sikorski, Jarek; Fagel, Nathalie ULg

in Geochronometria (2010)

Two chemical treatments for lead-210 measurement were compared on the sub-surface samples of a core from an ombrotrophic bog from East Belgium. The classical procedure involves a concentrated acid ... [more ▼]

Two chemical treatments for lead-210 measurement were compared on the sub-surface samples of a core from an ombrotrophic bog from East Belgium. The classical procedure involves a concentrated acid extraction of polonium. However, this treatment represents substantial health risks together with unknowns regarding both the degree of cleanliness and the Po extraction rate, and most importantly, is rather time consuming. We developed here an improved procedure involving an ashing step prior to acid extraction. This allows substantial improvements such as: 1/ the use of a relatively small amount of acid compared to the classical procedure and 2/ the substantial reduction of a total sample digestion time. Measurements of 210Pb concentrations were conducted by alpha spectrometry. Results show a good agreement of unsupported 210Pb activity obtained for both procedures, although some unknowns remain concerning the adsorption of 210Po on the plastic test tube, the volatilization of a small amount of ash, or the absorption of alpha particle at the alpha source surface. This however should not affect the 210Pb measurement as all the samples are spiked prior to ashing (i.e. the recoveries are fully monitored). Through this study, we are suggesting researchers to follow this new procedure in order to increase safety, cleanliness, better recovery and substantial time gain. [less ▲]

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See detailDevelopment of Liquid-Flooded Scroll Compressor and Expander Models
Bell, Ian; Lemort, Vincent ULg; Braun, Jim et al

(2008, July)

The Liquid Flooded Ericsson Cooler (LFEC) is a gas refrigeration cycle that uses liquid flooding of both the compressor and the expander to achieve nearly isothermal compression and expansion processes as ... [more ▼]

The Liquid Flooded Ericsson Cooler (LFEC) is a gas refrigeration cycle that uses liquid flooding of both the compressor and the expander to achieve nearly isothermal compression and expansion processes as developed by Hugenroth et al. (2007). Efficiency of the LFEC strongly depends on the efficiencies of the compressor and the expander. It is hoped that the LFEC can be a commercially competitive cooling technology, but for these hopes to be realized, high efficiencies for the compressor and expander must be achieved. In order to optimize the design of the compressor and the expander, comprehensive models of both machines have been developed. Validation of the models and analysis of these machines are presented in two separate papers (Bell et al. 2008, Lemort et al. 2008). The models are mechanistic and allow investigation of the influence of design changes and operating conditions on performance. [less ▲]

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See detailDevelopment of Mass Spectrometry based Analytical Tools for Animal Venoms Characterization
Echterbille, Julien ULg

Doctoral thesis (2017)

Animal venoms and toxins are now recognized as major sources of bioactive molecules that may be tomorrow’s new drug leads. Their complexity and their potential as drug sources have been demonstrated by ... [more ▼]

Animal venoms and toxins are now recognized as major sources of bioactive molecules that may be tomorrow’s new drug leads. Their complexity and their potential as drug sources have been demonstrated by application of modern analytical technologies, which have revealed venoms to be vast peptide combinatorial libraries. Structural as well as pharmacological diversity is immense, and mass spectrometry is now one of the major investigative tools for the structural investigation of venom components. The present work is dedicated to the development of new mass spectrometry-based methodologies for the study of animal venoms. The first methodology depicts an original approach to sequence snake toxins in a higher throughput way after an orthogonal separation of the crude venom. The second analytical development is devoted to decipher disulfide connectivity in cone snails’ toxins through a combination of partial reduction/oxidation followed by ion mobility separation of the semi-reduced/oxidized species and CID fragmentation. The last part of this work is focused on the capture of ligands of nAChRs in complex mixtures of peptides, especially in cone snail venoms. A new ligand has been discovered and fully characterized. [less ▲]

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See detailDevelopment of membranar effects in frame beams: experimental and analytical investigations
Demonceau, Jean-François ULg; Luu, Nguyen Nam Hai; Jaspart, Jean-Pierre ULg

in EUROSTEEL 2008, 5th European Conference on Steel and Composite Structures (2008, September)

Recent events such as natural catastrophes or terrorism attacks have highlighted the necessity to ensure the structural integrity of buildings under exceptional events. According to Eurocodes and some ... [more ▼]

Recent events such as natural catastrophes or terrorism attacks have highlighted the necessity to ensure the structural integrity of buildings under exceptional events. According to Eurocodes and some different other national design codes, the structural integrity of civil engineering structures should be ensured through appropriate measures but, in most of the cases, no precise practical guidelines on how to achieve this goal are provided. A European RFCS project called “Robust structures by joint ductility” has been set up in 2004, for three years, with the aim to provide requirements and practical guidelines allowing to ensure the structural integrity of steel and composite structures under exceptional event through an appropriate robustness. The investigations performed at Liège University, as part of this European project, are mainly dedicated to the exceptional event “Loss of a column in a steel or steel-concrete composite building frame”; the main objective is to develop a simplified analytical procedure to predict the frame response further to a column loss. The development of this simplified procedure is detailed in two complementary PhD theses: the thesis of J.-F. Demonceau and the thesis of H.N.N. Luu. The present paper describes experimental and analytical studies carried out within the first PhD thesis. In particular, a simplified analytical method allowing the prediction of the frame response with account of the membranar effects is described. [less ▲]

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See detailDevelopment of microsatellite markers for the characterisation of Phaeoisariopsis griseola (bean angular leaf spot agent) populations in Central America.
Busogoro, Jean-Pierre; Duterme, Olivier; Lepoivre, Philippe ULg

in Plant Protection Science (2002), 38(Special 1),

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See detailDevelopment of mini scale-down platform based on the response of GFP microbial biosensors
Brognaux, Alison ULg; Neubauer, Peter; Twizere, Jean-Claude ULg et al

Poster (2012, May 18)

The basic principle adopted in our studies is to use substrate limitation responsive biosensors in order to detect spatial glucose heterogeneities inside industrial bioreactors (whole-cell biosensor ... [more ▼]

The basic principle adopted in our studies is to use substrate limitation responsive biosensors in order to detect spatial glucose heterogeneities inside industrial bioreactors (whole-cell biosensor). Indeed, such heterogeneities cause a lowering of the biomass yield and an increase of by-products concentration. In our previous works, green fluorescent protein reporters have been used as biosensors of the heterogeneities generated in a two compartment scale-down reactor. As there is a huge variety of available whole cell biosensor to characterize the impact of such heterogeneities at the biological level, there is a need for high-throughput cultivation tools in order to investigate the usefulness of a given microbial biosensor among a library comprising several thousands of clones. This work is based on this statement and aims to investigate the potentialities of a mini scale-down platform. Four green fluorescent protein (GFP) transcriptional reporters have been chosen in Escherichia coli: rpoS::gfp, uspA::gfp, csiE::gfp and yciG::gfp. The promoters rpoS and uspA are induced in response to a variety of stresses whereas the two other promoters, csiE and yciG, are supposed to be more specific in front of a glucose limitation. First, the response of these biosensors has been assessed in chemostat reactors. These kinds of experiments allow easier interpretation of responses of stress gene related to a glucose limitation since the extracellular conditions are constants and cells are renewed. Biosensors carrying the csiE and yciG promoters have exhibited an induction in function of the glucose limitation. Secondly, a scale-down platform has been tested with the same biosensors and two kinds of glucose addition mode. This scale-down platform involves high-throughput cultivation tools, i.e. in our case shake flask, equipped with non-invasive optical sensors for the monitoring of the dissolved oxygen profile in front of the glucose addition mode. The first system is based on a commercial package (Enbase) based on the enzymatic release of glucose in the medium. The Enbase system allows the generation of a very smooth glucose profile without any perturbations. For comparison purpose, we have also used an intermittent feeding that induces strong fluctuation at the level of the glucose and the dissolved oxygen concentration. The intermittent addition of glucose induces a slow down at the level of the GFP synthesis, suggesting that temporal accumulation of glucose inhibits the activity of the yciG and csiE promoters. In conclusion, the scale-down platform is able to reproduce the same kind of glucose fluctuations that encounters the cells in large-scale processes but not allows studying the impact of high-cell density culture on gene expression. [less ▲]

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See detailThe development of mirative no wonder-constructions
Brems, Lieselotte ULg; Davidse, Kristin; Matthijs, Lennart

Conference (2012, July)

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See detailThe development of mobility controls
Crutzen, Nathalie ULg; Qian, Wei

Conference (2015, March 26)

Detailed reference viewed: 31 (8 ULg)
See detailDevelopment of molecular tests for simultaneous detection of Erwinias spp infecting potato tubers in Belgium
Moh, Ahoussi Augustin ULg; Roussel, S.; Van mellaert, S. et al

Poster (2005)

Detailed reference viewed: 21 (3 ULg)
See detailDevelopment of molecular tests for the detection of Ilar and latent viruses and Banana Mild Mosaic Virus
Marbot, S.; Reichel, H.; Kummert, J. et al

Conference (2004)

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See detailDevelopment of molecular tests for the detection of ILAR and latent viruses in fruit trees.
Roussel, S.; Kummert, J.; Dutrecq, O. et al

in Communications in Agricultural and Applied Biological Sciences (2004), 69(4), 427-432

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See detailDevelopment of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health
Libert, Xavier ULg

Doctoral thesis (2017)

Currently, contamination of the indoor environment by fungi is suggested to be a public health problem, although scientific evidence on the causal link is still limited. The monitoring of indoor airborne ... [more ▼]

Currently, contamination of the indoor environment by fungi is suggested to be a public health problem, although scientific evidence on the causal link is still limited. The monitoring of indoor airborne fungal contamination is a common tool to help understanding the link between fungi in houses and respiratory problems. Classical monitoring methods, based on cultivation and microscopic identification, have some limitations. For example, uncultivable or dead fungi (“unknown” fraction) cannot be identified, although they could have an impact on human health. In this context, molecular tools seem to be a valuable alternative. In this PhD work, different molecular tools were developed, from simplex to multiplex, to detect and identify indoor airborne fungi. The goal was to improve the detection of fungal contaminants, including the “unknown” fraction, as compared to the currently used classical monitoring methods. The necessary air sampling and DNA extraction protocols, adapted to the downstream molecular monitoring methods have also been developed. Through the application of the developed tools to specific case studies, we aimed to improve the current knowledge on fungal contamination. At first, we developed a specific ITS-based SYBR®green real-time PCR (qPCR) assay for Aspergillus versicolor, a species frequently observed in indoor air and known to be allergenic. Additionally, an ITS-based qPCR assay was developed for the specific detection of Exophiala jeanselmei, a pathogenic yeast suspected to be a part of the “unknown fraction”. The performance of these qPCR methods was assessed. This comparison demonstrated that SYBR®green qPCR assays can be used as a molecular alternative for monitoring of contaminated samples while eliminating the need for culturing and thereby considerably decreasing the required analysis time. However, qPCR has some limitations especially concerning the discrimination of genetically close species and multiplexing. The first issue was addressed through the use of post-qPCR high resolution melting (HRM) analysis, providing a proof-of-concept for this approach, using 3 closely related Aspergillus, i.e., A. versicolor, Aspergillus creber and Aspergillus sydowii. This HRM tool will allow a more accurate monitoring of these closely related indoor air contaminants, thereby contributing to an improved insight in the causal link between the specific presence of these species and health issues. The multiplexing issue was overcome through a Luminex xMAP® assay, developed for the simultaneous detection of the 10 most frequently in indoor air found fungi. All the species identified with the classical method were also detected with the xMAP® assay, however in a shorter time frame, and using less sample material. This assay will improve the communication with the involved medical team and the patient. To provide scientific evidence for the causal link between indoor airborne fungi and health problems, the full diversity needs however to be identified. This cannot be achieved by using a targeted assay. Therefore, next generation sequencing (NGS) could offer a valuable alternative as an open approach multiplex monitoring method. An NGS-based metagenomics approach was used to investigate the “unknown” agents in air samples of offices in contact with air-conditioning reservoirs and showed the first detection of E. jeanselmei in indoor air. Finally, a metagenomics analysis was performed to investigate the indoor airborne fungal diversity in contaminated residences in Brussels where people with health problems were living. This demonstrated that NGS could contribute to improved data concerning the indoor airborne fungal diversity, as compared to the currently used classical methods. The methods developed in this PhD work and the insights obtained are a first step for a better understanding of the causal link between indoor airborne fungi and public health. [less ▲]

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