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See detailEngineering a Novel β-Lactamase by a Single Point Mutation
Jacob, F.; Joris, Bernard ULg; Dideberg, O. et al

in Protein Engineering (1990), 4(1), 79-86

beta-Lactamases are widespread and efficient bacterial enzymes which play a major role in bacterial resistance to penicillins and cephalosporins. In order to elucidate the role of the residues lying in a ... [more ▼]

beta-Lactamases are widespread and efficient bacterial enzymes which play a major role in bacterial resistance to penicillins and cephalosporins. In order to elucidate the role of the residues lying in a conserved loop of the enzymatic cavity of the active-site serine Streptomyces albus G beta-lactamase, modified proteins were produced by oligo-directed mutagenesis. Mutation of Asn116, which lies on one side of the active site cavity pointing to the substrate-binding site, into a serine residue resulted in spectacular modifications of the specificity profile of the enzyme. That replacement yielded an enzyme with a nearly unchanged activity towards good penicillin substrates. In sharp contrast its efficiency in hydrolysing cephalosporins was drastically reduced, the best substrates suffering the largest decrease in the second-order rate constant for serine acylation. In fact that single mutation generated a truly new enzyme behaving exclusively as a penicillinase, a situation which is never encountered to the same degree in any of the numerous naturally occurring variants of class A beta-lactamases. [less ▲]

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See detailEngineering and characterisation of chimeric CXCR4 and CXCR7 chemokine receptors
Szpakowska, Martyna ULg; Fievez, Virginie; Counson, Manuel et al

Poster (2012, January)

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See detailEngineering and manufacturing for biotechnology
Hofman, M.; Thonart, Philippe ULg

Book published by Kluwer academic publishers (2001)

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See detailEngineering and Overexpression of Periplasmic Forms of the Penicillin-Binding Protein 3 of Escherichia Coli
Fraipont, Claudine ULg; Adam, Maggy; Nguyen-Disteche, Martine et al

in Biochemical Journal (1994), 298(1), 189-195

Replacement of the 36 and 56 N-terminal amino acid residues of the 588-amino-acid-residue membrane-bound penicillin-binding protein 3 (PBP3) of Escherichia coli by the OmpA signal peptide allows export of ... [more ▼]

Replacement of the 36 and 56 N-terminal amino acid residues of the 588-amino-acid-residue membrane-bound penicillin-binding protein 3 (PBP3) of Escherichia coli by the OmpA signal peptide allows export of F37-V577 PBP3 and G57-V577 PBP3 respectively into the periplasm. The modified ftsI genes were placed under the control of the fused lpp promoter and lac promoter/operator; expression of the truncated PBP3s was optimized by varying the copy number of the recombinant plasmids and the amount of LacI repressor, and export was facilitated by increasing the SecB content of the producing strain. The periplasmic PBP3s (yield 8 mg/l of culture) were purified to 70% protein homogeneity. They require the presence of 0.25 M NaCl to remain soluble. Like the membrane-bound PBP3, they undergo processing by elimination of the C-terminal decapeptide I578-S588, they bind penicillin in a 1:1 molar ratio and they catalyse hydrolysis and aminolysis of acyclic thioesters that are analogues of penicillin. The membrane-anchor-free PBP3s have ragged N-termini. The G57-V577 PBP3, however, is less prone to proteolytic degradation than the F37-V577 PBP3. [less ▲]

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See detailEngineering geological conditions in the central area of Shanghai
Schroeder, Christian ULg; Dassargues, Alain ULg; Li, Xiang Lin

in Bulletin of Engineering Geology & the Environment (1992), 46

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See detailEngineering geological mapping in Wallonia (Belgium) : present state and recent computerized approach
Delvoie, Simon ULg; Radu, Jean-Pol ULg; Ruthy, Ingrid ULg et al

Poster (2012, April)

An engineering geological map can be defined as a geological map with a generalized representation of all the components of a geological environment which are strongly required for spatial planning ... [more ▼]

An engineering geological map can be defined as a geological map with a generalized representation of all the components of a geological environment which are strongly required for spatial planning, design, construction and maintenance of civil engineering buildings. In Wallonia (Belgium) 24 engineering geological maps have been developed between the 70s and the 90s at 1/5,000 or 1/10,000 scale covering some areas of the most industrialized and urbanized cities (Liège, Charleroi and Mons). They were based on soil and subsoil data point (boring, drilling, penetration test, geophysical test, outcrop. . . ). Some displayed data present the depth (with isoheights) or the thickness (with isopachs) of the different subsoil layers up to about 50 m depth. Information about geomechanical properties of each subsoil layer,useful for engineers and urban planners, is also synthesized. However, these maps were built up only on paper and progressively needed to be updated with new soil and subsoil data. The Public Service of Wallonia and the University of Liège have recently initiated a study to evaluate the feasibility to develop engineering geological mapping with a computerized approach. Numerous and various data (about soil and subsoil) are stored into a georelational database (the geotechnical database – using Access, Microsoft®). All the data are geographically referenced. The database is linked to a GIS project (using ArcGIS, ESRI®). Both the database and GIS project consist of a powerful tool for spatial data management and analysis. This approach involves a methodology using interpolation methods to update the previous maps and to extent the coverage to new areas. The location (x, y, z) of each subsoil layer is then computed from data point. The geomechanical data of these layers are synthesized in an explanatory booklet joined to maps. [less ▲]

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See detailEngineering Geology Conditions of the Central Area of Shanghai : Preparation of the data for subsidence modelling
Dassargues, Alain ULg; Schroeder, Christian ULg; Monjoie, Albéric ULg

in Sixth International Congress of the International Association of Engineering Geology, Amsterdam (1990, August)

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See detailEngineering Multiferroism in CaMnO3
Bhattacharjee, Satadeep; Bousquet, Eric ULg; Ghosez, Philippe ULg

in Physical Review Letters (2009), 102

Structural instabilities of CaMnO3 are investigated from first principles. We point out that, on top of a strong antiferrodistortive instability responsible for its orthorhombic ground state, the cubic ... [more ▼]

Structural instabilities of CaMnO3 are investigated from first principles. We point out that, on top of a strong antiferrodistortive instability responsible for its orthorhombic ground state, the cubic perovskite structure of CaMnO3 also exhibits a weak ferroelectric instability. Although ferroelectricity is suppressed by antiferrodistortive motions, we show that it can be favored using strain or chemical engineering in order to make CaMnO3 multiferroic. We finally highlight that the ferroelectric instability of CaMnO3 is Mn-dominated. This illustrates that, contrary to common belief, ferroelectricity and magnetism are not necessarily exclusive but can be driven by the same cation. [less ▲]

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See detailEngineering the stability of a cold-active alpha-amylase
D'Amico, Salvino; Gerday, Charles ULg; Feller, Georges ULg

Poster (1999)

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See detailEngineering three-dimensional chains of porous nanoballs from a 1,2,4-triazole-carboxylate supramolecular synthon
Naik, A. D.; Dǐrtu, M. M.; Léonard, Alexandre ULg et al

in Crystal Growth & Design (2010), 10(4), 1798-1807

Glycine ethyl ester was recruited in an amine exchange process based on a transamination to afford ethyl 4H-1,2,4-triazol-4-yl-acetate (L1). The acid hydrolysis of this molecule leads to quantitative ... [more ▼]

Glycine ethyl ester was recruited in an amine exchange process based on a transamination to afford ethyl 4H-1,2,4-triazol-4-yl-acetate (L1). The acid hydrolysis of this molecule leads to quantitative isolation of 4H-1,2,4-triazol-4-yl acetic acid (L2). This versatile synthon crystallizes in a noncentrosymmetric orthorhombic space group (Fdd2) with Z = 16. This crystal structure is the first one for a 1,2,4-triazole ligand constructed from an amino acid derivative. The strong intermolecular hydrogen bonding O-HN (2.570(3)Å) connects molecules into infinite one-dimensional chains running parallel to the b axis, and the structure is further extended by numerous but moderate strength hydrogen bonds (C-HO). Prominent features of L2 are the presence of diverse potential coordinating groups such as carboxylic acid and triazole on the same framework as well as the inherent flexibility of the ligand backbone. Reaction of L1 or L2 with aq. Cu(BF 4) 2 in aq. DMF gives dark blue crystals which crystallize in a noncentrosymmetric, cubic space group (I4̌3m) and which were formulated as [Cu 3(μ 3-O)(L2) 3(H 2O) 3]BF 4H 2O (C1). The self-assembly of C 3-symmetric, μ 3-oxo bridged triangular tricopper secondary building blocks (SBB) formed an unique architecture which encompasses voluminous nanoball voids of 1 nm. The total solvent accessible volume is 4477.5 Å 3 which accounts for 48% of the cell volume. The crystal network stability was studied by thermogravimetric analysis (TGA)'differential thermal analysis (DTA) and scanning electron microscopy (SEM) analyses. Sorption properties and gas storage capacities were measured by BET. C1 shows no preference for N 2(g), but a reversible H 2(g) uptake of 21 cm 3/g was observed. Morphology analysis by SEM on single crystals of C1 shows "ultrawellś of square shape irregularly located on the surface, whose origin is due to desolvation or crystal defects. Mercury porosimetry measurements reveal pore size distribution with a diameter ranging from 350 nm to 2.3 μm © 2010 American Chemical Society. [less ▲]

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See detailEnglish and French Canadians, Flemish and Francophone Belgians. The Linguistic Composition of the Canadian and Belgian Federal Cabinet
Reuchamps, Min ULg

Conference (2007, April 27)

In divided societies, such as Belgium and Canada, the federal principle and consociational power-sharing have been implemented in order to accommodate ethno-linguistic communities. The combination of ... [more ▼]

In divided societies, such as Belgium and Canada, the federal principle and consociational power-sharing have been implemented in order to accommodate ethno-linguistic communities. The combination of Daniel Elazar’s seminal work on federalism in plural societies and of Arend Lijphart’s on consociationalism offers a theoretical framework which can be usefully applied to study the distribution of power between the two main ethno-linguistic communities at the highest level of the state. Using the existing “model of points” and the author’s own “model of the weight of ministerial portfolios”, this paper explores the linguistic composition of the federal governments in both countries over the last thirty years (1970-2006). Whereas the Belgian Constitution has required since 1970 that the federal cabinet be staffed by an equal number of Flemish and Francophone ministers, the Canadian Constitution does not require such a linguistic parity and therefore leaves a greater freedom for the composition of the government. On this background, two logics may explain the linguistic composition in the two cases: a logic of transaction between the two main communities and a logic of representation. The paper opens, on the one hand, with the elaboration of the theoretical framework combining the works of Elazar and Lijphart and, on the other hand, with the construction of the two models. The framework and the models are applied to each case, emphasizing the logic of transaction and the logic of representation. On the basis of this examination, this paper concludes by suggesting that Flemish and Francophone Belgians as well and English and French Canadians benefit from the composition of the federal cabinet. The conclusion opens—new—ways and means to hinder political tensions in plural societies through the institutional arrangement. [less ▲]

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See detailEnglish and Spanish Size Noun-constructions, a locus of ongoing grammaticalization
Brems, Lieselotte ULg; Verveckken, Katrien

Conference (2008)

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See detailEnglish and Spanish Size Noun-constructions, a locus of ongoing grammaticalization
Brems, Lieselotte ULg; Verveckken, Katrien

Conference (2008)

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See detailEnglish language immersion in french-speaking children : does learning to read have an impact on the subsequent acquisition of french orthographic code ?
Nicolay, Anne-Catherine ULg; Fantauzzi, A.; Sadzot, Agnès ULg et al

Poster (2006, May 19)

Apprendre à lire dans une langue n’interfère pas avec l’apprentissage de la lecture dans une autre langue mais prépare plutôt le terrain pour apprendre à lire dans une autre langue. Comme dans le langage ... [more ▼]

Apprendre à lire dans une langue n’interfère pas avec l’apprentissage de la lecture dans une autre langue mais prépare plutôt le terrain pour apprendre à lire dans une autre langue. Comme dans le langage oral, il peut y avoir quelques « mixing » de prononciation au niveau des mots qui disparaissent progressivement au fur et à mesure que les langues se compartimentalisent. Avec le temps, l’enfant apprendra également que des mots qui s’écrivent de la même manière dans les deux langues peuvent se prononcer de manière différente. Une recherche menée sur des enfants francophones Belges suivant un programme d’immersion scolaire en anglais et ayant appris à lire en anglais confirment ces observations. 150 enfants âgés de 7 à 12 ans fréquentant ce programme ont été testés sur leurs compétences en lecture du français (décodage et compréhension). Leurs performances (type et nombre d’erreurs, vitesse de lecture) ont été comparées à celles d’enfants francophones fréquentant un enseignement unilingue. Leurs habiletés métaphonologiques (manipulation de la rime et du phonème) ont également été testées. [less ▲]

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See detailEnglish Renaissance Lyrics
Delrez, Marc ULg

Learning material (2004)

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