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See detailDevelopment of an experimental equipment for the identification of constitutive laws
Flores, Paulo; Rondia, Etienne ULg; Habraken, Anne ULg

in International Journal of Forming Processes (2005), (Special Issue), 117-137

An experimental testing equipment for sheet samples is built in order to identify material parameters ofcomplex phenomenological constitutive laws. This equipment consists in a bi-axial test machine able ... [more ▼]

An experimental testing equipment for sheet samples is built in order to identify material parameters ofcomplex phenomenological constitutive laws. This equipment consists in a bi-axial test machine able to perform plane strain and simple shear tests separately or simultaneously and a Miyauchi simple shear test device. An optical extensometer is used to identify the strain field. The article is focused on the validation of the results of this new equipment by comparing with results obtained by finite elements (FE) simulations of already identified materials. The results are satisfactory and the bases for further material identification are established. [less ▲]

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See detailDevelopment of An Expert System for the Identification of Nonlinear Vibrating Systems
Dimitriadis, Grigorios ULg; Vio, Gareth Arthur; Shi, Dongfeng

in Brennan, M. J.; Liguore, S.; Mace, B. R. (Eds.) et al Proceedings of the IX International Conference on Recent Advances in Structural Dynamics (2006, July)

The aim of the present work is to attempt to create a logical framework to be used for the identification of nonlinear systems. It is assumed that no single identification method is general enough to work ... [more ▼]

The aim of the present work is to attempt to create a logical framework to be used for the identification of nonlinear systems. It is assumed that no single identification method is general enough to work with a significant range of systems. Therefore, this framework is based on the development of an Expert System that will detect and quantify the nonlinearity present in a given dynamical system and, subsequently, determine and apply the most suitable nonlinear system identification method or methods. [less ▲]

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See detailDevelopment of an extended Drucker-Prager hardening model for cross-anisotropy of soft rocks
François, Bertrand; Collin, Frédéric ULg; Dizier, Arnaud et al

in Pietruszczak, S.; Pande, G.N. (Eds.) PROCEEDINGS OF THE 2 nd INTERNATIONAL SYMPOSIUM ON COMPUTATIONAL GEOMECHANICS (COMGEO II) (2011)

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See detailDevelopment of an FDS-SAFIR interface
Tondini, Nicola; Franssen, Jean-Marc ULg

Scientific conference (2011, November 15)

A weak coupling strategy from CFD models to F.E. models is being developed on the base of an interface file. The software FDS and SAFIR are used in the applications.

Detailed reference viewed: 101 (6 ULg)
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See detailDevelopment of an immobilisation device for treatment of patients after breast conserving surgery for mammary carcinoma.
COUCKE, Philippe ULg; WONNER, Michel ULg; JANVARY, Zsolt Levente ULg et al

Poster (2010, September)

To develop an immobilisation device for prone positioning for breast irradiation. The basic requirements for the device are: rapid and accurate patient (re)positioning, compatibility with modern imaging ... [more ▼]

To develop an immobilisation device for prone positioning for breast irradiation. The basic requirements for the device are: rapid and accurate patient (re)positioning, compatibility with modern imaging devices and easy to handle by the treating technologists. [less ▲]

Detailed reference viewed: 39 (5 ULg)
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See detailDevelopment of an implantable integrated MEMS pressure sensor using polyimide epoxy composite and Atomic Layer Deposition for encapsulation
Gembaczka, P; Görtz, M; Kraft, Michael ULg

in Proceedings of "48th DGBMT Annual Conf. : Hannover, Germany Octobre 2014" (2014, October)

Detailed reference viewed: 23 (0 ULg)
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See detailDevelopment of an in vitro culture method for heart-shaped embryo in Phaseolus vulgaris.
Mergeai, Guy ULg; Baudoin, Jean-Pierre ULg

in Annual report of the Bean Improvement Cooperative (1990), 33

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See detailDevelopment of an in vitro culture method of very immature embryos to assist wide interspecific hybridisation in Phaseolus.
Geerts, P.; Baudoin, Jean-Pierre ULg; Mergeai, Guy ULg

in 3rd European conference on grain legumes : Opportunities for high quality, healthy and added-value crops to meet European demands, 14-19 November 1998, Valladolid, Spain (1998)

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See detailDevelopment of an in vitro culture techniques adapted to Phaseolus polyanthus and P. vulgaris embryos.
Geerts, P.; Baudoin, Jean-Pierre ULg; Mergeai, Guy ULg

in Bean Improvement Cooperative (1998), 41

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See detailDevelopment of an in vitro endotoxin test for monoolein-water liquid crystalline gel for use as an implant
Ouédraogo, Moustapha; Semdé, Rasmané; Somé, Issa T. et al

in Tropical Journal of Pharmaceutical Research [=TJPR] (2009), 8(6), 501-508

Purpose : Drugs that are administered by parenteral route must be apyrogenic. The aim of this study was to develop an in vitro endotoxin test for liquid crytalline gels for use as implants, using a ... [more ▼]

Purpose : Drugs that are administered by parenteral route must be apyrogenic. The aim of this study was to develop an in vitro endotoxin test for liquid crytalline gels for use as implants, using a monoolein-water liquid crystalline gel as a model. Methods : The gel-clot technique was used. The gel was dissolved first in isopropyl myristate, and the endotoxins were extracted with water for bacterial endotoxin test. Tests for the labelled lysate sensitivity and interfering factors were performed to validate the developed method. The limit of detection of endotoxin in the gel was also determined. Results : The labelled lysate sensitivity was confirmed. It was not influenced by the presence of extracts from the gels. Endotoxins in the contaminated test gels were completely extracted. Endotoxin concentration in the tested gels was below the calculated threshold endotoxin level. Conclusion : A method to perform in vitro endotoxins test of liquid crystalline gels was successfully developed and validated. Application of the technique to gels currently being developed in our laboratories indicate that the gels were apyrogenic. [less ▲]

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See detailDevelopment of an in vitro model for mercury exposure in marine mammals
Dupont, Aurélie ULg; Bouquegneau, Jean-Marie ULg; Siebert, Ursula et al

Poster (2007, November 01)

Detailed reference viewed: 21 (6 ULg)
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See detailDevelopment Of An In Vitro Pod Culture Technique For Young Pods Of Phaseolus Vulgaris L.
Geerts, P.; Sassi, K.; Mergeai, Guy ULg et al

in In Vitro Cellular & Developmental Biology-Plant (2000), 36(6),

Detailed reference viewed: 16 (6 ULg)
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See detailDevelopment of an in vivo phosphorylation system in Escherichia coli
Goux, Marine ULg

Poster (2013, October)

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as zirconium-89 (89Zr, t½=78.4h) or gallium-68 (68Ga, t½=1.13h)[a ... [more ▼]

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as zirconium-89 (89Zr, t½=78.4h) or gallium-68 (68Ga, t½=1.13h)[a]. Despite their important role in the therapeutic world, antibodies have many disadvantages related to their structure. In addition to their limited stability, the presence of disulfide bridges and their post-translational modifications make their production difficult and expensive. Moreover, conjugation of chelating agent often occurs on lysines, which is non-regioselective and leads to a heterogeneous mixture of products. In addition, the long clearance of antibodies can be a problem to obtain a good contrast when they are used in imaging. To address these different limitations, we developed a chemistry-free chelating system consisting of a highly phosphorylatable peptide tag. A specific phosphorylation step, with the alpha subunit of the casein kinase II (CKIIα), generates a nanocluster of 4 phosphates that can interact strongly with metal ion like zirconium. This strategy has already demonstrated its powerfulness for the stable and specific anchoring of protein on zirconium phosphonate-based microarray[b]. We are now adapting the use of this labeling tag to the stereoselective chelation of radionuclides for PET imaging. To ensure an efficient targeting of the radionuclide, the tag was genetically fused to a Nanofitin®, a protein scaffold developed as an alternative to antibodies. Nanofitins share the advantages of being small (7KDa), cystein-free, easy to engineer, highly stable both to temperature and pH and expressed with high yield in Escherichia coli. As described previously, functionalizing the tag with a phosphate nanocluster is a key step to provide it with the capacity to specifically chelate metal ions such as zirconium or gallium. In early development the phosphorylation was realized in vitro. In order to save purification steps and decrease production costs, we developed an in vivo phosphorylation system consisting in the co-expression of a Nanofitin® fused to the phosphorylatable tag and the CKIIα. Effectiveness of the in vivo system was demonstrated together by electrophoretic mobility shift assay and staining with a specific phosphoprotein staining gel: ProQDiamond®. To further optimize and validate the system, the rate of phosphorylation is evaluated by an isoelectric focusing method. This technique is based on the fact that the pHi of the Nanofitin® decreases as the protein is phosphorylated. Such a technique allowed us to isolate the different phosphorylation states of the phospho-Nanofitin® (from 0 to 4 phospho-serine). In parallel to the development of the in vivo system, we are developing a phosphoprotein purification protocol by immobilized metal affinity chromatography, relying on the specific interaction of phosphate moeity with gallium(III) or iron(III). The use of a single tag for both the purification and the labeling will provide a much simpler and straightforward system. Finally this fusion system of phosphate nanocluster and a Nanofitin® will allows us to combine the advantages of an original single step regioselective labeling for both zirconium-89 and gallium-68, together with a highly stable targeting moiety. [a] Heuveling D. A. et al. (2011), Oral Oncology, 47, pp. 2–7 [b] Cinier M. et al. (2012), Journal of Biological Inorganic Chemistry, 17, pp. 399–407 [less ▲]

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See detailDevelopment of an integrated modelling methodology for compartment fires by implementing a weak coupling strategy between a CFD and an FE software
Tondini, Nicola; Franssen, Jean-Marc ULg

in De Luca, Antonello (Ed.) XXIII giornate italiane della costruzione in acciaio (2011, October 12)

This article presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a ... [more ▼]

This article presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a finite element (FE) software applied to structural systems. In particular, a weak coupling methodology use to simulate a fire exposed structure by modelling the fire development in the compartment, the heat penetration in the structure and the mechanical response is described. The advantages and the disadvantages of such a technique are highlighted compared to a full coupling that conversely takes into account all mutual interactions. This methodology is particularly suitable for relatively thin steel structures in large compartments. Moreover, the need of a standardised transfer file in order to get the methodology as flexible as possible in term of versatility is emphasized. [less ▲]

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See detailDEVELOPMENT OF AN INTEGRATED STRATEGY FOR CONTROLLING THE ALLERGEN ISSUE IN THE BELGIAN FOOD AND CATERING INDUSTRY - ALLERRISK
De Loose, Marc; Daeseleire, Else; Taverniers, Isabelle et al

Report (2009)

Detailed reference viewed: 37 (2 ULg)
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See detailDevelopment of an integrated tool for solar buildings design and renewal.
Andre, Philippe ULg; Nicolas, Jacques ULg

in Proceedings PLEA 96 International conference (1996, September)

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See detailDevelopment of an interface between a CFD software and a FE software for compartment fires
Tondini, Nicola; Franssen, Jean-Marc ULg

in Costruzioni Metalliche (2012), 3

The article presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a ... [more ▼]

The article presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a Finite Element (FE) software applied to structural systems. In particular, a weak coupling methodology used to simulate a fire exposed structure by modelling the fire development in the compartment, the heat penetration in the structure and the mechanical response is described. A practical application is also shown. [less ▲]

Detailed reference viewed: 64 (3 ULg)