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See detailEffects of brand presence and stimulus of comparison on response inhibition toward alcohol cues in male and female heavy drinkers
Kreusch, Fanny ULg; Quertemont, Etienne ULg

in 2010 annual meeting of the Belgian Association for Psychological Sciences (2010)

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See detailEffects of Brassicaceae secondary metabolites on the Glutathione S-transferase activity from Episyrphus balteatus De Geer (Diptera: Syrphidae)
Vanhaelen, N.; Haubruge, Eric ULg; Francis, Frédéric ULg

in Pesticide Biochemistry & Physiology (2001), 71(3), 170-177

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See detailEffects of Caesarean section on behaviour of Belgian Blue cows and their calves.
Nicks, Baudouin ULg; Desiron, Alain; Vandenheede, Marc ULg et al

Article for general public (1999)

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See detailEffects of calmodulin on aromatase activity in the preoptic area.
Balthazart, Jacques ULg; Baillien, M.; Charlier, Thierry ULg et al

in Journal of Neuroendocrinology (2005), 17(10), 664-71

Oestrogens derived from the neural aromatisation of testosterone play a key role in the activation of male sexual behaviour in many vertebrates. Besides their slow action on gene transcription mediated by ... [more ▼]

Oestrogens derived from the neural aromatisation of testosterone play a key role in the activation of male sexual behaviour in many vertebrates. Besides their slow action on gene transcription mediated by the binding to nuclear receptors, oestrogens have now been recognised to have more rapid membrane-based effects on brain function. Rapid changes in aromatase activity, and hence in local oestrogen concentrations, could thus rapidly modulate behavioural responses. We previously demonstrated that calcium-dependent kinases are able to down-regulate aromatase activity after incubations of 10-15 min in phosphorylating conditions. In the present study, in quail hypothalamic homogenates, we show that Ca2+ or calmodulin alone can very rapidly change aromatase activity. Preincubation with 1 mM EGTA or with a monoclonal antibody raised against calmodulin immediately increased aromatase activity. The presence of calmodulin on aromatase purified by immunoprecipitation and electrophoresis was previously identified by western blot and two consensus binding sites for Ca2+-calmodulin are identified here on the deduced amino acid sequence of the quail brain aromatase. The rapid control of brain aromatase activity thus appears to include two mechanisms: (i) an immediate regulatory process that involves the Ca2+-calmodulin binding site and (ii) a somewhat slower phosphorylation by several protein kinases (PKC, PKA but also possibly Ca2+-calmodulin kinases) of the aromatase molecule. [less ▲]

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See detailEffects of Capsaicin on the Endothelial Permeability in Isolated and Perfused Rabbit Lungs
Delaunois, Annie ULg; Gustin, Pascal ULg; Ansay, Michel ULg

in Fundamental & Clinical Pharmacology (1993), 7(2), 81-91

Changes in pulmonary endothelial permeability and in microvascular hemodynamics in response to capsaicin (10(-4) M) were investigated in isolated, perfused rabbit lungs. Blood-free perfusate was ... [more ▼]

Changes in pulmonary endothelial permeability and in microvascular hemodynamics in response to capsaicin (10(-4) M) were investigated in isolated, perfused rabbit lungs. Blood-free perfusate was recirculated through ventilated lungs in an isogravimetric state, under zone III conditions with a constant flow. Using the occlusions method, the total pressure gradient between the arterial and the venous levels (delta Pt) was partitioned into four components: arterial (delta Pa), pre-(delta Pa') and post-(delta Pv') capillary, and venous (delta Pv). The capillary filtration coefficient (Kf,c) was evaluated by measuring the amount of fluid filtering through the endothelium when arterial and venous pressures were suddenly increased. Capsaicin caused no changes in the vascular pressures at any level of the pulmonary circulation but induced a significant 3-fold increase in the Kf,c (P < 0.05). This reaction was accompanied by pulmonary oedema. The mechanisms involved in the permeability changes were investigated by testing the capacity of different drugs to block the response to capsaicin. Clonidine (10(-7) M to 10(-5) M), morphine (10(-6) M), aspirin (10(-3) M), ketanserin (10(-8) M) and (+/)- CP 96,345 (10(-6) M), an antagonist of neurokinin NK1 receptor, completely prevented the effects of capsaicin on the Kf,c. In contrast, terfenadine (10(-7)) together with cimetidine (10(-5) M) had no protective effect against capsaicin. It was concluded that capsaicin-induced pulmonary oedema was due to an increase in the capillary filtration coefficient and not to hemodynamic changes. This alteration in the endothelium permeability is mediated by the release of endogenous peptides from C-fibers upon the action of capsaicin and subsequent activation of NK1 receptors, probably by substance P. Moreover, 5-hydroxytryptamine receptors and arachidonic acid derivates are also involved in this reaction [less ▲]

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See detailEffects of Castration and Testosterone Treatment on the Activity of Testosterone-Metabolizing Enzymes in the Brain of Male and Female Zebra Finches
Vockel, A.; Prove, E.; Balthazart, Jacques ULg

in Journal of Neurobiology (1990), 21(5), 808-25

Recently, we described the distribution of testosterone-metabolizing enzymes (i.e., aromatase, 5 alpha- and 5 beta-reductases) in the zebra finch (Taeniopygia guttata) brain using a sensitive radioenzyme ... [more ▼]

Recently, we described the distribution of testosterone-metabolizing enzymes (i.e., aromatase, 5 alpha- and 5 beta-reductases) in the zebra finch (Taeniopygia guttata) brain using a sensitive radioenzyme assay combined to the Palkovits punch method. A number of sex-differences in the activity of these enzymes were observed especially in nuclei of the song-control system. The hormonal controls of these differences have now been analyzed by gonadectomizing birds of both sexes and by giving them a replacement therapy with silastic implants of testosterone (T). Five nuclei of the song system (Area X [X], nucleus magnocellularis of the anterior neostriatum [MAN], nucleus robustus archistriatalis [RA], nucleus intercollicularis [ICo], hyperstriatum ventrale, pars caudalis [HVc]) and three preoptic-hypothalamic areas (preoptic anterior [POA], periventricular magnocellular nucleus [PVM], and posterior medial hypothalamic nucleus [PMH]) were studied as well as other limbic and control non-steroid-sensitive areas. The activity of the 5 alpha-reductase was higher in males than in females for the five song-control nuclei and was not affected by the hormonal treatments. The overall activity of this enzyme was not sexually dimorphic in POA and PVM. It was higher in males than in females in intact birds only, and was reduced by gonadectomy and enhanced by T. The activity of the 5 beta-reductase was higher in females than in males in all nuclei of the song system and in POA, but was not influenced by the changes in T level. Both sex and treatment effects were observed in the control of aromatase. The production of estrogens was dimorphic (females greater than males) in RA and PMH. It was increased by T in POA, PVM, and PMH, and also in RA. These data show that some of the sex differences in T-metabolizing enzymes result from the exposure to different levels of T in adulthood (e.g., 5 alpha-reductase in POA and PVM or aromatase in PVM), whereas others persist even if birds are exposed to the same hormonal conditions. These are presumably the result of organizational effects of steroids. The steroid modulation of the aromatase might be related directly to the activation of sexual, aggressive, and nest-building behaviors, whereas the stable dimorphism in 5 alpha- and 5 beta-reductase observed in the nuclei of the song system might be one of the neurochemical bases of the sex differences in the vocal behavior of the zebra finch. [less ▲]

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See detailEffects of castration on fear reactions of male sheep.
Vandenheede, Marc ULg; Bouissou, Marie-France

in Applied Animal Behaviour Science (1996), 47

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See detailEffects of cellular morphology on electricalconductivity of carbon nanotubes containing nanocomposites foams
Tran, Minh Phuong ULg; Thomassin, Jean-Michel ULg; Alexandre, Michaël et al

Conference (2012, June 07)

In the last few years, polymer carbon nanotubes (CNTs) nanocomposites foams have received increasing attention due to their potential applications in electrostatic dissipation (ESD) and in electromagnetic ... [more ▼]

In the last few years, polymer carbon nanotubes (CNTs) nanocomposites foams have received increasing attention due to their potential applications in electrostatic dissipation (ESD) and in electromagnetic interferences (EMI) shielding. To be efficient, these foams must exhibit appropriate electrical conductivity (> 1 S/m) and dielectric constant. A good understanding of the influence of the foam structural parameters on the electrical properties of the foam will ultimately enable the optimum design of these materials for the targeted applications. A wide range of poly(methyl methacrylate) (PMMA)/CNTs nanocomposites foams were synthesized using the supercritical CO2 technology. Different foaming parameters, such as the temperature, impregnation pressure, time and rate of depressurization were varied to modify the foam structure. The amount of carbon nanotubes in PMMA plays the most important role in increasing the electrical conductivity. Nanocomposite foams show higher electrical conductivity than non-foamed nanocomposites at the same volume content of CNTs. Effects of foam morphology such as cell-density; pore size, volume expansion, and cell-wall thickness on electrical conductivity were comprehensively assessed. High electrical conductivity can be achieved with nanocomposite foams that have high volume expansion, small pore size, high cell density, and thin cell-walls [less ▲]

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See detailEffects of cellular morphology on electricalconductivity of carbon nanotubes containing nanocomposites foams
Tran, Minh Phuong ULg; Detrembleur, Christophe ULg; Thomassin, Jean-Michel ULg et al

Conference (2012, September 13)

In the last few years, polymer carbon nanotubes (CNTs) nanocomposites foams have received increasing attention due to their potential applications in electrostatic dissipation (ESD) and in electromagnetic ... [more ▼]

In the last few years, polymer carbon nanotubes (CNTs) nanocomposites foams have received increasing attention due to their potential applications in electrostatic dissipation (ESD) and in electromagnetic interferences (EMI) shielding. To be efficient, these foams must exhibit appropriate electrical conductivity (> 1 S/m) and dielectric constant. A good understanding of the influence of the foam structural parameters on the electrical properties of the foam will ultimately enable the optimum design of these materials for the targeted applications. A wide range of poly(methyl methacrylate) (PMMA)/CNTs nanocomposites foams were synthesized using the supercritical CO2 technology. Different foaming parameters, such as the temperature, impregnation pressure, time and rate of depressurization were varied to modify the foam structure. The amount of carbon nanotubes in PMMA plays the most important role in increasing the electrical conductivity. Nanocomposite foams show higher electrical conductivity than non-foamed nanocomposites at the same volume content of CNTs. Effects of foam morphology such as cell-density; pore size, volume expansion, and cell-wall thickness on electrical conductivity were comprehensively assessed. High electrical conductivity can be achieved with nanocomposite foams that have high volume expansion, small pore size, high cell density, and thin cell-walls [less ▲]

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See detailEffects of cellulose, pectin and guar gum on plasma insulin and metabolites in resting dogs
Istasse, Louis ULg; de Haan, Véronique; Beckers, Jean-François ULg et al

in Proceedings of the Nutrition Society (1990)

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See detailEffects of central administration of Naloxone on the extinction of appetitive sexual responses
Holloway, Kevin; Cornil, Charlotte ULg; Balthazart, Jacques ULg

in Behavioural Brain Research (2004), 153(2), 567-572

Several studies indicate that opioids are involved in the control of consummatory sexual behavior in male Japanese quail. Naloxone has been reported to increase copulatory responses. In the current study ... [more ▼]

Several studies indicate that opioids are involved in the control of consummatory sexual behavior in male Japanese quail. Naloxone has been reported to increase copulatory responses. In the current study, the effect of naloxone on appetitive sexual behaviors was assessed during extinction test trials. Naloxone was found to substantially reduce appetitive responding, suggesting that opioids differentially affect anticipatory and contact components of sexual behavior. [less ▲]

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See detailEffects of centrally versus peripherally administered ethanol in C57BL/6J and CD1 mice
Tambour, Sophie ULg; Didone, Vincent ULg; Quertemont, Etienne ULg et al

in Behavioural Pharmacology (2005), 16

Locomotor activation is often reported to occur after a systemic administration of low doses of ethanol in most mouse strains, as for example outbred CD1 mice. However, in some strains of mice, such as ... [more ▼]

Locomotor activation is often reported to occur after a systemic administration of low doses of ethanol in most mouse strains, as for example outbred CD1 mice. However, in some strains of mice, such as the inbred C57BL/6J mice, and in rats, systemic injections of ethanol typically induce only a depression of the locomotor activity. Recently, Correa et al. (2003) showed that direct infusions of ethanol in the brain ventricles of rats induced locomotor stimulant effects. These authors suggested that some undefined peripheral effects of ethanol may mask its central stimulant effects when ethanol is administered intraperitoneally. The aim of the present study was to investigate the locomotor effects of either intraperitoneal and intracerebroventricular ethanol administrations in two strains of mice, outbred CD1 and inbred C57BL/6J, that are respectively characterized by the presence and absence of a locomotor stimulant response to ethanol. The results showed that ethanol at moderate and high doses induced locomotor depressant effects in C57BL/6J mice whatever the route of ethanol administration. In contrast, ethanol induced a biphasic effect on locomotor activity in CD1 mice with a stimulant response at low doses followed by a significant sedation. Such a response to ethanol was observed after both peripheral and central administrations of ethanol. The results of the present study demonstrate that the locomotor effects of ethanol in mice are not affected by the route of administration, i.e. peripheral or central. In these rodents, there is no evidence that unidentified peripheral effects of ethanol mask the stimulant ethanol effects. [less ▲]

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See detailEffects of cerebral injury on transgenic mice expressing the human HSP70
Plumier, Jean-Christophe ULg; Arsmtrong, John Norman; Robertson, Harold A. et al

Poster (1995)

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See detailEffects of Changes in Nutritional Conditions on Timing of Puberty: Clinical Evidence from Adopted Children and Experimental Studies in the Male Rat
Bourguignon, Jean-Pierre ULg; Gerard, Arlette ULg; Alvarez Gonzalez, Maria-Luz ULg et al

in Hormone Research (1992), 38 Suppl 1(NULL), 97-105

Among 32 patients with idiopathic central precocious puberty seen during a 3-year period, 1/4 were adopted children from developing countries who showed early sexual maturation during the catch-up process ... [more ▼]

Among 32 patients with idiopathic central precocious puberty seen during a 3-year period, 1/4 were adopted children from developing countries who showed early sexual maturation during the catch-up process following their arrival in Belgium. To study the possible mechanism accounting for such clinical observations, we used the male rat as a model, and evaluated the effect of variations in early nutritional conditions, by manipulating litter size, on hypothalamic and testicular maturation. We had shown previously that, in the male rat, onset of puberty was preceded, between 15 and 25 days of age, by a transiently increased activation of N-methyl-D-aspartate receptors involved in a facilitatory control of pulsatile secretion of gonadotropin-releasing hormone. We also showed that the proportion of elongated spermatids in testicular cell homogenates increased between 25 and 45 days of age. When compared to pups of a small litter (6/dam), those of a large litter (14/dam) showed a reduced growth rate (1.9 vs. 3.5 g/day) before weaning (21 days), whereas they grew at a similar rate (5.6 vs. 4.7 g/day) after weaning. At 35 days of age, the animals raised in the large litter showed evidence of delayed hypothalamic and testicular maturation when compared to animals from the small litter. Reduction of litter size at 17 days allowed food-restricted pups of a large litter to resume a normal growth rate before weaning.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detaileffects of cholecaciferol supplementation and optimized calcium intakes on vitamin D status, muscle strength and bone haelth: a one-year pilot randomized controlled trial in adults with severe burns
ROUSSEAU, Anne-Françoise ULg; FOIDART-DESSALLE, Marguerite ULg; LEDOUX, Didier ULg et al

in Burns : Journal of the International Society for Burn Injuries (in press)

Objective: Burn patients are at risk of hypovitaminosis D and osteopenia or sarcopenia. Vitamin D pleiotropic effects may influence bone and muscle health. The aim of this pilot study was to assess ... [more ▼]

Objective: Burn patients are at risk of hypovitaminosis D and osteopenia or sarcopenia. Vitamin D pleiotropic effects may influence bone and muscle health. The aim of this pilot study was to assess effects of a cholecalciferol (VD3) supplementation and an optimized calcium (Ca) regimen on vitamin D (VD) status, bone and muscle health during sequelar stage of burn injury. Design: Monocentric randomized controlled trial. Methods: Fifteen adults with thermal burns dating from 2 to 5 years were randomized into two groups. For 12 months, they either received a quarterly IM injection of 200,000 IU VD3 and daily oral Ca (Group D) or placebo (Group P). VD status and bone remodeling markers were assessed every 3 months. Knee muscle strength and bone mineral density were, respectively, assessed using isokinetic dynamometry and dual X-ray absorptiometry at initiation (M0) and completion (M12) of the protocol. Results: Of all the patients, 66% presented with VD deficiency and 53% (with 3 men <40 y) were considered osteopenic at inclusion. After one year, calcidiol levels significantly increased in Group D to reach 40 (37–61) ng/ml. No significant change in bone health was observed in both groups while Group D significantly improved quadriceps strength when tested at high velocity. Conclusions: This VD3 supplementation was safe and efficient to correct hypovitaminosis D in burn adults. When combined with optimized Ca intakes, it demonstrated positive effects on muscle health but not on bone health. A high prevalence of hypovitaminosis D and osteopenia in these patients, as well as their wide range of muscle performances, seem to be worrying when considering rehabilitation and quality of life. [less ▲]

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See detailEffects of chondroitin sulfate and interleukin-1 beta on human articular chondrocytes cultivated in clusters
Bassleer, C. T.; Combal, J. P.; Bougaret, S. et al

in Osteoarthritis and Cartilage (1998), 6(3), 196-204

OBJECTIVE: To test the effects of chondroitin sulfate (ACS, a glycosaminoglycan of cartilage) with and without interleukin-1 beta (IL-1 beta) on human articular chondrocytes cultivated in clusters and in ... [more ▼]

OBJECTIVE: To test the effects of chondroitin sulfate (ACS, a glycosaminoglycan of cartilage) with and without interleukin-1 beta (IL-1 beta) on human articular chondrocytes cultivated in clusters and in long-term (0-16 days or 16-32 days). DESIGN: Chondrocyte productions of proteoglycans (PGs), type II collagen (coll-II) and prostaglandin E2 (PGE2) were assayed by specific radioimmunoassays applied to conditioned culture media and to clusters. RESULTS: During the two culture periods (0-16 days or 16-32 days), ACS (100-1000 micrograms/ml) increased total PG production and had no effect on the production of coll-II by chondrocytes. During the first 16 days, ACS (500-1000 micrograms/ml) decreased total PGE2 synthesis. IL-1 beta decreased PG and coll-II productions and increased PGE2 synthesis. During the first period (0-16 days), while the cluster is forming, ACS counteracted the IL-1 beta-induced effects on PG (500-1000 micrograms ACS/ml), coll-II (100-1000 micrograms ACS/ml) and PGE2 (500-1000 micrograms ACS/ml) productions. During the second period (16-32 days), when the cluster is already formed, ACS counteracted the IL-1 beta-induced effects on total PG (100-1000 micrograms ACS/ml), coll-II (1000 micrograms ACS/ml) and PGE2 (1000 micrograms ACS/ml) productions. CONCLUSION: These in vitro studies suggest that ACS is able to increase matrix component production by human chondrocytes and to inhibit the negative effects of IL-1 beta. [less ▲]

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See detailEffects of chondroitin sulfate on the gene expression profile in IL-1β stimulated synovial fibroblast cells cultures
Lambert, Cécile ULg; Dubuc, Jean-Emile; Montell, E. et al

in Osteoarthritis and Cartilage (2013, April), 21(Supplement April 2013),

Purpose: Chondroitin sulfate (CS) is one the most used molecules in the management of OA. In this study, we performed a microarray analysis and identified a differential expression profile between control ... [more ▼]

Purpose: Chondroitin sulfate (CS) is one the most used molecules in the management of OA. In this study, we performed a microarray analysis and identified a differential expression profile between control and IL-1β stimulated synovial fibroblast cells cultures. In a second step, we investigated the effects of CS on this gene expression profile. Methods: OA synovial specimens were obtained from 12 patients undergoing knee replacement. At the surgery time, the synovial membrane was dissected. Synovial fibroblast cells (SFC) were enzymatically isolated and used after four passages (P4). SFC were pre-treated 1 hour with highly purified bovine CS (200 µg/ml, Bioibérica S.A., Barcelona, Spain) before treatment with IL-1β (1 ng/ml) for 24 hours. Total RNA was extracted using the RNeasy Mini Kit. RNA purity and quality were evaluated using the Experion RNA StdSens Analysis kit (Bio-rad Laboratories). Gene expression profiling was performed using Illumina’s multi-sample format Human HT-12 BeadChip (Illumina Inc.). Differential analysis was performed with the BRB array tools software. Class comparison test between control (Ctl) and interleukin (IL)-1β conditions, Ctl and Ctl/CS and IL-1β and IL-1β/CS conditions was based on paired t-test where Ctl and IL-1β, Ctl and Ctl/CS and IL-1β and IL-1β/CS were paired for each patient. The biological relevance of up- and down-regulated genes was analyses with Ingenuity Pathways Analysis (Ingenuity® Systems). Probes with a p-value below 0.001 were chosen and classified as up- or down-regulated ones. Results: 3308 genes were identified as differentially expressed genes between Ctl and IL-1β conditions. We observed a differential profile of expression of major pathways involved in OA pathogenesis. The key identified pathways were related to inflammation, complement cascade, angiogenesis, cartilage catabolism and anabolism and Wnt signaling. In the inflammatory network, the most upregulated cytokines were IL-8 and IL-6 with a fold change of 156.25 and 58.8 respectively. We also identified several chemokines, enzymes and metallothioneins (MTs). Complement factor B (CFB) and complement component 3 (C3) are two factors upregulated in the inflammatory complement cascade. We also identified some genes implicated in the angiogenesis pathway. The most upregulated was Stanniocalcin 1 (STC1) with a fold change of 9.09. The differential expression of intermediates involved in both cartilage anabolism and catabolism was revealed by the IL-1β stimulation, showing an imbalance in favour of catabolism. MMP-3 was largely upregulated (fold change of 62.5). Wnt 5A and low density lipoprotein receptor-related protein (LRP8) were significantly upregulated while frizzled homolog 2 (FZD2) and dickkopf homolog 3 (DKK3) were downregulated in the Wnt signaling pathway. We next performed a class comparison test between Ctl and Ctl/CS in one hand and IL-1β and IL-1β/CS on the other hand. 660 genes were identified as differentially expressed between Ctl and Ctl/CS conditions while 241 genes were identified between IL-1β and IL-1β/CS. Among them, our attention was focused on two genes upregulated in the presence of CS: lysyl oxidase-like 4 (LOXL4) and claudin 11 (CDLN11), two genes that negatively regulate cell invasion. Conclusions: We here evidenced in synovial fibroblast cells the modulation of gene expression following IL-1β stimulation. We also demonstrated the modulatory effects of CS on gene expression and isolated several CS-modulated genes of interest such as LOXL4 and CDLN11, which could constitute new mechanisms of action of the molecule and contribute to explain the symptomatic efficacy of CS in the treatment of OA. [less ▲]

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See detailEFFECTS OF CHONDROITIN SULFATE ON THE GENE EXPRESSION PROFILE IN INTERLEUKIN-1Β STIMULATED SYNOVIAL FIBROBLAST CELLS CULTURES
Lambert, Cécile ULg; Dubuc, Jean-Emile; Montell, E et al

Conference (2013, November 23)

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