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See detailDevelopment of a quantitative immunocapture real-time PCR assay for detecting structurally intact adenoviral particles in water
Ogorzaly, Leslie; Bonot, Sébastien; Elmoualij, Benaïssa ULg et al

in Journal of Virological Methods (2013), 194

Development of rapid, sensitive and specific methods for detection of infectious enteric viruses in water is challenging but crucial for gaining reliable information for risk assessment. An immunocapture ... [more ▼]

Development of rapid, sensitive and specific methods for detection of infectious enteric viruses in water is challenging but crucial for gaining reliable information for risk assessment. An immunocapture real-time PCR (IC-qPCR) was designed to detect jointly the two major viral particle components, i.e. the capsid protein and the viral genome. Targeting both constituents helps circumventing the technical limits of cell culture approaches and the inability of PCR based methods to predict the infectious status. Two waterborne pathogenic virus models, human adenovirus types 2 and 41, were chosen for this study. IC-qPCR showed a detection limit of 10 MPNCU/reaction with a dynamic range from 102 to 106 MPNCU/reaction. Sensitivity was thus 100-fold higher compared to ELISA-based capture employing the same anti-hexon antibodies. After optimisation, application on environmental water samples was validated, and specificity towards the targeted virus types was obtained through the qPCR step. Heat-treated pure samples as well as surface water samples brought evidence that this method achieves detection of encapsidated viral genomes while excluding free viral genome amplification. As a consequence, adenovirus concentrations estimated by IC-qPCR were below those calculated by direct qPCR. The results demonstrate that the IC-qPCR method is a sensitive and rapid tool for detecting, in a single-tube assay, structurally intact and thus potentially infectious viral particles in environmental samples. [less ▲]

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See detailDevelopment of a quantitative method to detect trace amounts of hazelnut and soy allergens in food
Dobson, Rowan ULg; Fourdrilis, Séverine; Kirsch, Stéphanie ULg et al

Conference (2009)

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See detailDevelopment of a quantitative risk assessment for cheese made from raw goat milk contaminated by Listeria monocytogenes
Delhalle, Laurent ULg; Ellouze, Mariem; Clinquart, Antoine ULg et al

Poster (2011, September)

A retrospective study was performed to assess the potential risk of human listeriosis following a contamination by L. monocytogenes of cheeses made from goat raw milk reported by the Belgian Federal ... [more ▼]

A retrospective study was performed to assess the potential risk of human listeriosis following a contamination by L. monocytogenes of cheeses made from goat raw milk reported by the Belgian Federal Agency for the Safety of the Food Chain in 2005. The source of the contamination was related to a shedder goat, excreting 2.6 log cfu (colonies forming units) L. monocytogenes / ml without any clinical symptom. On the basis of the collected data, a quantitative microbial risk assessment model was developed covering the production chain from the milking of goats until the consumed products. Predictive microbiology models were used to simulate the growth of L. monocytogenes during the process of cheeses made from goat raw milk. The modular exposure assessment model showed a significant growth of L. monocytogenes during chilling and storage of the milk collected the day before the cheese production (increase of 1.7 log cfu/ml for the median) and during the step of starter and rennet adjunction to milk (increase of 0.8 log cfu/ml for the median). The median estimated final result (in the fresh cheese) was equal to 3.5 log cfu/g. The model estimates (expressed as median final result issued from the exposure assessment) were realistic compared to the number of L. monocytogenes measured in the fresh cheese (3.6 log cfu/g) reported during the cheese contamination period. The average number of expected cases of human listeriosis was between 0 and 1 for a high-risk sub-population and 0 for a low-risk healthy sub-population. Scenario analysis was finally performed to identify the most significant factors and aid in developing priorities for risk mitigation. Thus, by using quantitative risk assessment and predictive microbiology models, this study provided valuable information to identify and to control critical steps in a local production chain of goat cheese made from raw milk. [less ▲]

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See detailDevelopment of a Quick Quantitative Real-Time PCR for the In Vivo Detection and Quantification of Peach latent mosaic viroid
Parisi, Olivier ULg; Lepoivre, Philippe ULg; Jijakli, Haissam ULg

in Plant Disease (2011), 95(2), 137-142

Viroids are plant pathogens infecting a broad range of herbaceous and tree crops. Among them, the Peach latent mosaic viroid (PLMVd) infects mainly peach trees, causing a loss of production with no ... [more ▼]

Viroids are plant pathogens infecting a broad range of herbaceous and tree crops. Among them, the Peach latent mosaic viroid (PLMVd) infects mainly peach trees, causing a loss of production with no curative options. Detecting this viroid is thus important for certification procedures aiming to avoid the release of infected material into orchards. Presented here is a complete detection method based on reverse transcription (RT) followed by a quantitative real-time polymerase chain reaction (PCR). New primers were selected and optimal reaction conditions determined for routine application of the method. The technique is 105 times more sensitive than the endpoint RT-PCR used for PLMVd detection, and permits earlier detection of PLMVd in infected plants. The quick, low-cost extraction procedure used and the quality of the results obtained make this method suitable for routine testing. [less ▲]

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See detailDevelopment of a radioimmunoassay for bovine chymosin B
Idrissa-Sidikou, Djibo; Remy, Benoit; Dufrasne, Isabelle ULg et al

in Tropicultura (2007), 25(3), 134-139

The present study was conducted to develop and validate a specific radioimmunoassay system for measurement of bovine chymosin B (bChyB) concentrations in plasma samples. Bovine ChyB was used for ... [more ▼]

The present study was conducted to develop and validate a specific radioimmunoassay system for measurement of bovine chymosin B (bChyB) concentrations in plasma samples. Bovine ChyB was used for immunization of rabbits and as standard and tracer. Chymosin B concentrations were measured in plasma samples from two groups of calves (Group 1: calves sampled from birth to 24 hours; Group 2: calves sampled from Day 1 to 21 after birth) and from one cow during the peri-partum period. Detection limit of the assay was 9.0 ng/mL. Recovery was higher than 89.3%. Repeatability and reproducibility ranged from 1.52% to 5.23% and from 1.52% to 12.57% respectively. No cross-reaction was found with pepsinogen A from bovine, porcine or human origins. In Group 1, bChyB concentrations increased from 47.3±45.1 ng/mL (5 min after birth) to 325.5±161.2 ng/mL (12 hours after birth), then it decreased till 293.0±161.5 ng/mL (24 hours after birth). In Group 2, concentrations decreased from Day 1 (455.3±191.1 ng/mL) to Day 21 (117.9±85.1 ng/mL). In adult cow, mean concentration was 136.0±32.3 ng/mL. In conclusion, bChyB is able to cross the stomach basal membrane and to reach the blood circulation at detectable levels in both young calves and adult cows. [less ▲]

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See detailDevelopment of a Radioimmunoassay for Bovine Pepsinogen A
Sidikou, I. D.; Remy, B.; Beckers, Jean-François ULg

in Revue d'Elévage et de Médecine Vétérinaire des Pays Tropicaux (2005), 58(4), 229-235

Pepsinogen A is the most abundant zymogen found in blood, and its enzymatic measurement is used for the diagnosis of gastric lesions. The present study was conducted to develop a radioimmunoassay (RIA ... [more ▼]

Pepsinogen A is the most abundant zymogen found in blood, and its enzymatic measurement is used for the diagnosis of gastric lesions. The present study was conducted to develop a radioimmunoassay (RIA) specific to pepsinogen A in bovine plasma. The authors purified large amounts of three non-denatured isoforms of bovine pepsinogen A with high proteolytic activity. These homogeneous preparations were used to produce specific antisera in New Zealand White rabbits, and three antisera with high titers were obtained. In the present assay the antiserum #822 was used at a final dilution of 1/250,000. The detection limit of the assay was 20 ng/ml and the recovery ranged from 85.5 to 103.3%. The repeatability (intra-assay coefficient of variation) was lower than 6.6%, whereas the reproducibility (inter-assay coefficient of variation) was lower than 13.4%. The capacity of the RIA to detect pepsinogen A in blood was tested by measuring the concentrations in plasma of newborn calves (n = 6) serially sampled from birth to four months of age. The mean pepsinogen A value (mean ± standard deviation) in the plasma of calves was 2071 ± 752 ng/ml one day after birth. The concentration decreased progressively and was about 1196 ± 307 ng/ml at day 21, and 677 ± 109 ng/ml at day 120. The present study is the first report on pepsinogen A concentrations in bovine measured by RIA. Further investigations using the RIA should be performed in order to confirm these values and determine pepsinogen levels in older cattle in physiological and pathological conditions such as gastrointestinal helminthosis [less ▲]

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See detailDevelopment of a RAPD marker and a semi-selective medium for aureobasidium pullulans (strain Ach1-1), a biocontrol agent against postharvest disease on apples
El Hamouchi, A.; Najimi, B.; Achbani, E. H. et al

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (2007), 30

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See detailDevelopment Of A Rapid Rt-Pcr Test For The Detection Of Peach Latent Mosaic Viroid, Pear Blister Canker Viroid, Hop Stunt Viroid And Apple Scar Skin Viroid In Fruit Trees From Tunisia
Hassen, If.; Roussel, S.; Kummert, J. et al

in Journal of Phytopathology (2006), 154(4), 217-223

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See detailDevelopment of a Reference Database for Particle-Induced Gamma-ray Emission spectroscopy
Dimitriou, P.; Becker, H.-W.; Bogdanović-Radović, I. et al

in Nuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms (2016), 371

Particle-Induced Gamma-ray Emission (PIGE) is a powerful analytical technique that exploits the interactions of rapid charged particles with nuclei located near a sample surface to determine the ... [more ▼]

Particle-Induced Gamma-ray Emission (PIGE) is a powerful analytical technique that exploits the interactions of rapid charged particles with nuclei located near a sample surface to determine the composition and structure of the surface regions of solids by measurement of characteristic prompt γ rays. The potential for depth profiling of this technique has long been recognized, however, the implementation has been limited owing to insufficient knowledge of the physical data and lack of suitable user-friendly computer codes for the applications. Although a considerable body of published data exists in the nuclear physics literature for nuclear reaction cross sections with γ rays in the exit channel, there is no up-to-date, comprehensive compilation specifically dedicated to IBA applications. A number of PIGE cross-section data had already been uploaded to the Ion Beam Analysis Nuclear Data Library (IBANDL) (http://www-nds.iaea.org/ibandl) by members of the IBA community by 2011, however a preliminary survey of this body of unevaluated experimental data has revealed numerous discrepancies beyond the uncertainty limits reported by the authors. Using the resources and coordination provided by the IAEA, a concerted effort to improve the situation was made within the Coordinated Research Project on the Development of a Reference Database for PIGE spectroscopy, from 2011 to 2015. The aim of the CRP was to create a data library for Ion Beam Analysis that contains reliable and usable data on charged particle γ-ray emission cross sections that would be made freely available to the user community. As the CRP has reached its completion, we shall present its main achievements, including the results of nuclear cross-section evaluations and the development of a computer code that will become available to the public allowing for the implementation of a standardless PIGE technique. © 2015 Elsevier B.V. All rights reserved. [less ▲]

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See detailDevelopment of a regenerable filter aid for beverages
Bonacchelli, B.; Zgoulli, S.; Harmegnies, F. et al

Poster (1998, May 08)

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See detailDevelopment of a regenerable filtration aid for beverages.
Bonacchelli, B.; Zgoulli, S.; Harmegnies, F. et al

Poster (1997, August)

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See detailDevelopment of a regional-scale library of near infrared reflectance soil spectra for alternative assessment of soil characteristics in Southern Belgium
Genot, Valérie ULg; Dardenne, Pierre; Bock, Laurent ULg et al

Poster (2012)

In Walloon Region (Southern Belgium), five routine soil laboratories are grouped within a network promoting a better quality in analysis (www.requasud.be). The harmonization of protocols as well as ... [more ▼]

In Walloon Region (Southern Belgium), five routine soil laboratories are grouped within a network promoting a better quality in analysis (www.requasud.be). The harmonization of protocols as well as methodological or technical prospective are realized under scientifically supervision of our research laboratory. In this context, a study was conducted to evaluate the ability of the NIRS to predict some soil properties: CEC, TOC, TN and clay content. The initial models were elaborated upon local PLS regression on set of 1 300 soil samples. The local PLS calibration used allows an accurate prediction of the soil properties and precision of NIRS technique is comparable to reference analytical method [less ▲]

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See detailThe development of a safety culture: a fanciful hope in the health care sector ?
COUCKE, Philippe ULg

in Belgian Journal of Medical Oncology [=BJMO] (2014), 8(3), 66-71

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See detailDevelopment Of A Scar Marker And A Semi-Selective Medium For Specific Quantification Of Pichia Anomala Strain K On Apple Fruit Surfaces
De Clercq, Deborah; Cognet, Stephane; Pujol, Marta et al

in Postharvest Biology & Technology (2003), 29(3), 237-247

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See detailDevelopment of a scoring system to assess lameness status in dairy cattle farms
Frisee, Vincent ULg; Guyot, Hugues ULg

in Société Belge Francophone de Buiatrie & Vlaamse vereniging voor Buiatrie (Ed.) Proceeding of the 6th European Congress of Bovine Health Management (2011, September)

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See detailDEVELOPMENT OF A SCROLL EXPANDER FOR MICRO-CSP WITH ORGANIC RANKINE CYCLE
Dechesne, Bertrand ULg; Orosz, M.S.; Legros, Arnaud ULg et al

in Dechesne, Bertrand; Orosz, M.S.; Legros, Arnaud (Eds.) et al DEVELOPMENT OF A SCROLL EXPANDER FOR MICRO-CSP WITH ORGANIC RANKINE CYCLE (2012)

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See detailDevelopment of a self-administrated quality of life questionnaire for sarcopenia in elderly subjects: the SarQol
Beaudart, Charlotte ULg; Biver, Emmanuel; Reginster, Jean-Yves ULg et al

in Age & Ageing (2015), 44

BACKGROUND: the impact of sarcopenia on quality of life is currently assessed by generic tools. However, these tools may not detect subtle effects of this specific condition on quality of life. OBJECTIVE ... [more ▼]

BACKGROUND: the impact of sarcopenia on quality of life is currently assessed by generic tools. However, these tools may not detect subtle effects of this specific condition on quality of life. OBJECTIVE: the aim of this study was to develop a sarcopenia-specific quality of life questionnaire (SarQoL, Sarcopenia Quality of Life) designed for community-dwelling elderly subjects aged 65 years and older. SETTINGS: participants were recruited in an outpatient clinic in Liège, Belgium. SUBJECTS: sarcopenic subjects aged 65 years or older. METHODS: the study was articulated in the following four stages: (i) Item generation-based on literature review, sarcopenic subjects' opinion, experts' opinion, focus groups; (ii) Item reduction-based on sarcopenic subjects' and experts' preferences; (iii) Questionnaire generation-developed during an expert meeting; (iv) Pretest of the questionnaire-based on sarcopenic subjects' opinion. RESULTS: the final version of the questionnaire consists of 55 items translated into 22 questions rated on a 4-point Likert scale. These items are organised into seven domains of dysfunction: Physical and mental health, Locomotion, Body composition, Functionality, Activities of daily living, Leisure activities and Fears. In view of the pretest, the SarQoL is easy to complete, independently, in ∼10 min. CONCLUSIONS: the first version of the SarQoL, a specific quality of life questionnaire for sarcopenic subjects, has been developed and has been shown to be comprehensible by the target population. Investigations are now required to test the psychometric properties (internal consistency, test-retest reliability, divergent and convergent validity, discriminant validity, floor and ceiling effects) of this questionnaire. [less ▲]

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