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See detailEffects of thiol reagents on Streptomyces K15 DD-peptidase-catalysed reactions
Leyh-Bouille, Mélina; Nguyen-Distèche, Martine ULg; Bellefroid-Bourguignon, Catherine et al

in Biochemical Journal (1987), 241(3), 893-897

The 26,000-Mr DD-peptidase of Streptomyces K15 binds one equivalent of thiol reagents as 5,5'-dithiobis-(2-nitrobenzoate) or p-chloromercuribenzoate (pCMB). Derivatization of the DD-peptidase by pCMB ... [more ▼]

The 26,000-Mr DD-peptidase of Streptomyces K15 binds one equivalent of thiol reagents as 5,5'-dithiobis-(2-nitrobenzoate) or p-chloromercuribenzoate (pCMB). Derivatization of the DD-peptidase by pCMB decreases the efficacy of the initial binding of the ester carbonyl donor Ac2-L-Lys-D-Ala-D-lactate to the enzyme (K), the rate of enzyme acylation by the donor (K+2) and the rate of enzyme deacylation (k+3). However, the value of the k+2/k+3 ratio, and therefore the percentage of total enzyme which, at saturating concentrations of the donor, is present as acyl-enzyme at the steady state of the reaction, are not modified. The enzyme's binding sites for pCMB and benzylpenicillin are not mutually exclusive. But, when compared with the native enzyme, the pCMB-derivatized enzyme undergoes acylation by benzylpenicillin with a decreased second-order-rate constant (k+2/K) value and gives rise to a penicilloyl adduct of increased stability. Since the acyl-enzyme mechanism is not annihilated by pCMB derivatization, it is proposed that basically, and like all the other DD-peptidases/penicillin-binding proteins so far characterized, the Streptomyces K15 DD-peptidase is an active-site-serine enzyme. [less ▲]

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See detailEffects of three avocado/soybean unsaponifiable mixtures on human chondrocytes metabolism
Henrotin, Yves ULg; Labasse, A; Zheng, SX et al

in Arthritis and Rheumatism (1996), 39

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See detailEffects of three avocado/soybean unsaponifiable mixtures on metalloproteinases, cytokines and prostaglandin E2 production by human articular chondrocytes.
Henrotin, Yves ULg; Labasse, A. H.; Jaspar, J. M. et al

in Clinical Rheumatology (1998), 17(1), 31-9

The in-vitro effects of avocado and soybean unsaponifiable residues on neutral metalloproteinase activity, cytokines and prostaglandin E2 (PGE2) production by human articular chondrocytes were ... [more ▼]

The in-vitro effects of avocado and soybean unsaponifiable residues on neutral metalloproteinase activity, cytokines and prostaglandin E2 (PGE2) production by human articular chondrocytes were investigated. Avocado and soybean unsaponifiable residues were mixed in three ratios: 1:2 (A1S2), 2:1 (A2S1) or 1:1 (A2S2). Freshly isolated human chondrocytes were cultured for 72 h in the absence or presence of interleukin-1beta, (IL-1beta) (17 ng/ml), with or without unsaponifiable residue mixtures at a concentration of 10 microg/ml. A/S unsaponifiable residues were also tested separately at concentrations of 3.3, 6.6 and 10 microg/ml. All A/S unsaponifiable mixtures reduced the spontaneous production of stromelysin, interleukin-6 (IL-6), interleukin-8 (IL-8) and prostaglandin E2 (PGE2) by chrondrocytes. At concentrations of 3.3 and 6.6 microg/ml, A/S residues, tested separately, were potent inhibitors of the production of IL-8 and PGE2. Nevertheless, only avocado residue inhibited IL-6 production at these concentrations. A/S unsaponifiable mixtures had a more pronounced inhibitory effect on cytokine production than avocado or soybean residues added alone. As anticipated, IL-1beta induced a marked release of collagenase, stromelysin, IL-6, IL-8 and PGE2. A/S unsaponifiable mixtures partially reversed the IL-1 effects on chrondrocytes. These findings suggest a potential role for A/S unsaponifiable extracts in mitigating the deleterious effects of IL-1beta: on cartilage. [less ▲]

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See detailEffects of thromboxane A2 receptor antagonists and COX inhibitors on osteogenic sarcome cell-induced platelet aggregation
De Leval, X.; Delarge, J.; Neven, P. et al

Poster (2001, June 01)

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See detailEffects of thromboxane A2 receptor antagonists and thromboxane synthase inhibitors on osteogenic sarcoma cell induced platelet aggregation
De Leval, X.; Benoît, V.; Neven, P. et al

Poster (2002, October)

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See detailEffects of thromboxane A2 receptor antagonists and thromboxane synthase inhibitors on osteogenic sarcoma cell induced platelet aggregation
De Leval, X.; Benoît, V.; Neven, P. et al

Poster (2002, May)

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See detailEffects of thromboxane A2 receptor antagonists and thromboxane synthase inhibitors on osteogenic sarcoma cell-induced platelet aggregation
De Leval, X.; Benoit, V.; Neven, P. et al

in Fundamental & Clinical Pharmacology (2002), 16

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See detailEffects of thromboxane A2 receptor antagonists and thromboxane synthase inhibitors on osteogenic sarcoma cell-induced platelet aggregation
De Leval, X.; Benoit, V.; Neven, P. et al

Poster (2002, March 02)

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See detailEffects of thromboxane A2 receptor antagonists and thromboxane synthase inhibitors on osteogenic sarcoma cell-induced platelet aggregation
De Leval, X.; Benoit, V.; Neven, P. et al

in Fundamental & Clinical Pharmacology (2002)

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See detailEffects of tillage practices and crop residue exportation on earthworm communities and soil physico-­chemical properties in silt loam arable soil (Belgium)
Lemtiri, Aboulkacem ULg; Colinet, Gilles ULg; Alabi, Taofic ULg et al

Poster (2014, June 08)

Earthworms are known to play integral roles in soils, and are often referred to as vital soil and ecosystem engineers due to their capacity to influence a wide range of chemical, physical, and biological ... [more ▼]

Earthworms are known to play integral roles in soils, and are often referred to as vital soil and ecosystem engineers due to their capacity to influence a wide range of chemical, physical, and biological properties of soil environments. Therefore, it is important to understand how earthworm communities are impacted by tillage systems and crop management practices. In the present study, earthworm and soil samples were collected from wheat cultivated fields in Gembloux, Belgium under the following four experimental treatments: (1) conventional tillage with crop residues left in the soil (CT/IN); (2) conventional tillage with crop residues removed from the field (CT/OUT); (3) reduced tillage with crop residues left in the soil (RT/IN); and (4) reduced tillage with crop residues removed from the field (RT/OUT). The different tillage systems were applied for four consecutive years prior to the initiation of the current study. Results indicated soil compaction was significantly higher in RT compared with CT up to a depth of 6–49 cm. Significant differences were not detected between residue incorporation depth systems, where results showed mean earthworm abundance was respectively 182.25 and 180 individuals.m-2 in CT and RT. Mean earthworm biomass was similarly not significantly different between CT and RT, where results were respectively 48.52 and 57.27 g.m-2. However, a significant difference was observed between IN and OUT treatments, suggesting the exportation of wheat residues will limit earthworm abundance and biomass in CT and RT plots. Data showed high representation of the endogeic earthworm ecological category, notably N. c. caliginosus regardless of treatment. Despite tillage system and exportation of crop residues, N. c. caliginosus, L. terrestris, and A. r. rosea exhibited high abundance, indicating tolerance to soil environmental conditions. For each depth, measures of soil physico-chemical properties showed significant differences among treatments. Furthermore, soil chemical property attributes were significantly higher in the first soil centimetres compared to lower soil layers. These results were attributable to earthworm activity and wheat residues, suggesting earthworms contributed to nutrient dynamics, particularly at increased soil depths. Overall, the results emphasise the influence of exportation of crop residues on earthworm community and also, the important influence of earthworm activity on soil physico-chemical properties change, processes which are closely linked. [less ▲]

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See detailEffects of time of day on age-related differences in cognitive tests.
Schmitz, Xavier ULg; Willems, Sylvie ULg; Meulemans, Thierry ULg

Poster (2009, June 03)

Previous studies have shown a shift in the circadian rhythm – and more particularly in the optimal time of day (OTD) – across the adult life span (May et al., 1993). The aim of this study was to ... [more ▼]

Previous studies have shown a shift in the circadian rhythm – and more particularly in the optimal time of day (OTD) – across the adult life span (May et al., 1993). The aim of this study was to investigate the relationship between cognitive efficiency and OTD in 113 healthy old adults (Age: M = 69, SD = 6.1, Range = 60-80) and 175 younger adults (M = 40.8, SD = 12.9, Range = 20-59). Participants performed a large battery of cognitive tests that assessed episodic memory, working memory, executive and attentional functions. Results on the MEQ (Morningness-Eveningness Questionnaire; Horne & Östberg, 1976) confirmed the age-related shift toward a self-reported morning preference in older adults. Second, the categorization of participants according to their MEQ scores and the time of testing revealed that the OTD has a greater impact upon cognitive performance in older than in younger adults. Third, the age-related OTD impact was more striking in working memory (Brown-Peterson and Pasat) and episodic memory tasks (Buschke) than in other aspects of the cognitive functioning. In conclusion, older participants tested during their peak circadian periods tend to show greater performance on memory tasks that require careful or strategic processing relative to older participants who are tested at off-peak times of day. Taken together, these findings indicate that care must be taken when investigators are considering the effects of age on effortful memory tasks, which are particularly modulated by OTD in older adults. [less ▲]

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See detailEffects of trace metals on mouse B16 melanoma cells in culture
Bahbouth, Eliane; Siwek, Brigitte; De Pauw-Gillet, Marie-Claire ULg et al

in Biological Trace Element Research (1993), 36(2), 191-201

The effects of fourteen metal ions on B16 cell proliferation, differentiation and melanogenesis have been analysed.

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See detailEffects of training on delayed onset muscle soreness following isokinetic eccentric exercise
Croisier, Jean-Louis ULg; Camus, Gérard; Ledent, M. et al

in Archives of Physiology & Biochemistry (1999, September), 107(suppl september 1999), 101

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See detailEffects of Training on Exercise-Induced Muscle Damage and Interleukin 6 Production
Croisier, Jean-Louis ULg; Camus, G.; Venneman, Ingrid ULg et al

in Muscle & Nerve (1999), 22(2), 208-12

To address the question of whether the increased plasma concentration of interleukin 6 (IL-6) following strenuous muscular work could be related to exercise-induced muscle damage, 5 moderately active male ... [more ▼]

To address the question of whether the increased plasma concentration of interleukin 6 (IL-6) following strenuous muscular work could be related to exercise-induced muscle damage, 5 moderately active male volunteers underwent two isokinetic exercise sessions in the eccentric mode, separated by a period of 3 weeks during which the subjects underwent five training sessions. Before training, exercise was followed by severe muscle pain (delayed-onset muscle soreness; DOMS), and by significant increases in plasma IL-6 level and serum myoglobin concentration (SMb) (P < 0.001). After training, postexercise DOMS and SMb values were significantly lower than those measured before training. There was no significant difference between plasma IL-6 levels measured at the same time points before and after training. We conclude that the hypothetical relationship between exercise-induced muscle damage and increased postexercise levels of circulating IL-6 is not substantiated by the present results. [less ▲]

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See detailEffects of training on myocellular enzyme leakage and delayed onset muscle soreness following maximal isokinetic eccentric exercise
Croisier, Jean-Louis ULg; Camus, Gérard; Duchateau, J. et al

in Mediators of Inflammation (1997), 6

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See detailThe effects of training on ventilation and blood gases in exercising Thoroughbreds
Roberts, C. A.; Marlin, D. J.; Lekeux, Pierre ULg

in Equine Veterinary Journal. Supplement (1999), 30

The effects of training on ventilation and blood gases during exercise were investigated in 6 clinically normal, detrained Thoroughbred horses. They underwent a 16 week training programme similar to the ... [more ▼]

The effects of training on ventilation and blood gases during exercise were investigated in 6 clinically normal, detrained Thoroughbred horses. They underwent a 16 week training programme similar to the type frequently used for Thoroughbred racehorses in Great Britain. Standardized treadmill exercise tests (2 min canter at 8 and 10 m/s C8 and C10[ and 2 min gallop at 12 m/s [G12], on a level surface) were performed prior to and after 16 weeks of training. Respiratory flow rates were measured using ultrasound flow transducers. Blood samples were drawn from a transverse facial artery and the right atrium. Minute ventilation, respiratory frequency and tidal volume were not significantly altered by training. Peak inspiratory flow rate was lower following training at 8 and 10 m/s, but not at 12 m/s. Arterial oxygen tension was decreased during trot and canter following training. Blood lactate concentration post G12 decreased following training (10.5 +/- 2.2 mmol/l vs. 7.7 +/- 2.2 mmol/l; P < 0.05). The increase in the degree of exercise-induced arterial hypoxaemia following training may reflect a lack of pulmonary adaptation to training in the face of improved cardiovascular and muscular function. [less ▲]

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See detailEffects of transcranial magnetic stimulation coil orientation and pulse width on short-latency afferent inhibition
Hannah, Ricci; D'Ostilio, Kevin ULg; Goetz, Stefan et al

Poster (2015, March)

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