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See detailDevelopment of a genetic evaluation for body condition score for Canadian Holsteins
Loker, S.; Bastin, Catherine ULg; Miglior, F. et al

in Journal of Dairy Science (2013), 96

Valacta (Sainte-Anne-de-Bellevue, Québec, Canada) is the Canadian Dairy Herd Improvement organization responsible for milk recording in Québec and Atlantic provinces. Up to 14 first-lactation body ... [more ▼]

Valacta (Sainte-Anne-de-Bellevue, Québec, Canada) is the Canadian Dairy Herd Improvement organization responsible for milk recording in Québec and Atlantic provinces. Up to 14 first-lactation body condition score (BCS) records were collected per cow (average of 2.5 records per cow), allowing the trait to be described by a random regression animal model so that animals could be ranked by the shape of their BCS curve. However, Valacta’s BCS are available from Québec herds only and the long-term objective of this research is to develop a nationwide genetic evaluation of sires and cows for BCS. Alternatively, Holstein Canada (Brantford, Ontario, Canada) collects type trait records nationwide, primarily for first-lactation cows. Holstein Canada typically collects a single record per trait, so that selection for Holstein Canada BCS would be based on overall BCS level rather than the shape of the BCS curve. Several different methods of genetically evaluating Valacta’s BCS were investigated, including consideration of average BCS level across lactation, the amount of fluctuation in the BCS curve during lactation, and combinations of BCS level and BCS fluctuation. Sires with ≥25 daughters were compared (as opposed to comparing cows) because their BCS estimated breeding values (EBV) are based on more information, and so should be more reliable. Of the different methods of calculating Valacta BCS EBV, ranking bulls based on overall BCS level gave the best results in that their daughter phenotypic BCS curves showed limited loss in early lactation BCS and replenished condition by the end of lactation. Whereas Valacta’s BCS were analyzed using a random regression animal model, Holstein Canada only needs to collect 1 BCS record per cow at classification and the resulting BCS EBV was strongly correlated with Valacta’s BCS EBV. Furthermore, because Holstein Canada’s BCS are collected nationally and Valacta’s BCS are not, a national genetic evaluation for Holstein Canada’s BCS is more convenient. The results of this study do not eliminate the possibility of a genetic evaluation of BCS as a longitudinal trait, but indicate that other methods of calculating Valacta BCS EBV should be explored. Until that time, genetically evaluating Holstein Canada’s BCS is simple, easily implemented, and may be effective in altering the level and shape of the genetic BCS curve. [less ▲]

Detailed reference viewed: 23 (7 ULg)
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See detailDevelopment of a genetic traceability test in pig based on single nucleotide polymorphism detection
Goffaux, F.; China, B.; Dams, Laureen ULg et al

in Forensic Science International (2005), 151(2-3), 239-247

In order to assure traceability along the meat transformation process, a powerful system is required. The administrative traceability shows limits that the use of genetic markers could overcome. The ... [more ▼]

In order to assure traceability along the meat transformation process, a powerful system is required. The administrative traceability shows limits that the use of genetic markers could overcome. The individual genomes contain sequence differences, basis of the genetic polymorphism of which the genetic markers are the witnesses. Among them, two classes seem to dominate on the traceability field: the microsatellites and the single nucleotide polymorphisms (SNP). The aim of this work was to develop a genetic traceability test in pig based on SNPs mainly located in 5' and 3' untranslated regions (UTRs). A set of 21 SNP markers including new SNPs identified in this study and SNPs previously described was selected. A genotyping assay was performed on 96 individuals representing the major crossbred of the pig population in Belgium. Results showed that all individuals tested presented a different genotype. This genotyping method might help the administrative system to guarantee the traceability of pork meat along the transformation process. (c) 2005 Elsevier Ireland Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 54 (6 ULg)
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See detailDevelopment of a genomic evaluation for milk production for a local bovine breed
Colinet, Frédéric ULg; Vandenplas, Jérémie ULg; Faux, Pierre ULg et al

in Book of Abstracts of the 64th Annual Meeting of the European Federation of Animal Science (2013, August)

Detailed reference viewed: 27 (11 ULg)
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See detailDevelopment of a high throughput de novo sequencing platform for peptidic toxins combining proteomics and transcriptomics
Degueldre, Michel ULg; Verdenaud, Marion; Zuniga, Sheila et al

Poster (2014, November 07)

Detailed reference viewed: 35 (3 ULg)
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See detailDevelopment of a high-dynamic range imaging instrument for a single telescope by a pupil remapping system
Kotani, Takayuki; Lacour, Sylvestre; Choquet, Elodie et al

in Optical and Infrared Interferometry II (2010, July 01)

We present the laboratory demonstration of a very high-dynamic range imaging instrument FIRST (Fibered Imager foR Single Telescope). FIRST combines the techniques for aperture masking and a single-mode ... [more ▼]

We present the laboratory demonstration of a very high-dynamic range imaging instrument FIRST (Fibered Imager foR Single Telescope). FIRST combines the techniques for aperture masking and a single-mode fiber interferometer to correct wavefront errors, which leads to a very high-dynamic range up to 106 around very near the central object (~ λ/D) at visible to near-infrared wavelengths. Our laboratory experiments successfully demonstrated that the original image can be reconstructed through a pupil remapping system. A first on-sky test will be performed at the Lick Observatory 3- m Shane telescope for operational tests in the summer of 2010. [less ▲]

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See detailDevelopment of a high-order interior penalty discontinuous Galerkin method for compressible turbulent flows. Application to Reynolds-averaged Navier-Stokes and large eddy simulations
Drosson, Marcus ULg

Doctoral thesis (2013)

Over the last decade several studies and workgroups came to the conclusion that high-order discretisation schemes are the most suited to overcome the limitations of today’s flow solvers. The latter are at ... [more ▼]

Over the last decade several studies and workgroups came to the conclusion that high-order discretisation schemes are the most suited to overcome the limitations of today’s flow solvers. The latter are at most second-order accurate and therefore often deliver poor results on currently used meshes. Within this context, discontinuous Galerkin (DG) methods have recently been identified as having the biggest potential for future flow solvers. Further research is however needed to improve the computational efficiency and the robustness of these schemes. Both issues are faced in the subsequent chapters of the thesis. The first part of the thesis is devoted to the solution of the Reynolds-averaged Navier-Stokes (RANS) equations using an interior penalty (IP) formulation. Whereas the intention is not to develop new turbulence models, the focus lies on the adaptation of existing models to the particular features of high-order discretisation schemes. In contrast to classical low-order methods, high-order schemes are prone to Gibbs oscillations which – if no care is taken – can lead to the breakdown of the algorithm. Given the stability issues caused by negative values of the eddy viscosity, different changes of the one-equation Spalart-Allmaras (S-A) model are discussed. Whilst having identical sparsity patterns as the popular second Bassi-Rebay scheme (BR2), the simpler IP formulation is up to 30% less time-consuming. Unfortunately, its stability strongly depends on a seemingly more or less arbitrary penalty parameter. As this parameter also effects the conditioning of the resulting system of discretised equations, its choice is of utmost importance. This might explain why the IP formulation has received only little attention over the past decades. The thesis analyses for the first time the use of the interior penalty discretisation to solve a system of RANS equations. One of the principal contributions of this work is the generalisation of the penalty parameter to anisotropic meshes and a highly variable viscosity. Several numerical examples are presented to illustrate the effect of the newly proposed definition of the penalty parameter. The focus of the second part lies on turbulent boundary layer resolution. Based on detailed grid convergence analyses as a function of interpolation order, element type and grid size, clear guidelines for the choice of boundary layer meshes for practical applications are provided. Besides straight boundaries, the effect of boundary curvature or a hybrid discretisation are investigated. Moreover, the concept of “quasi-straight” elements is introduced. Following this innovative approach, the accuracy of the calculated shear friction can further be improved by a proper choice of the position of the inner-element nodes, without increasing the total number of unknowns. Finally, several numerical applications clearly demonstrate the potential of the method to solve industrial problems. In the last part, large eddy simulations of the Taylor-Green vortex and the decay of homogeneous isotropic turbulence are presented. This last chapter constitutes a first step towards the use of discontinuous Galerkin methods for large eddy simulations. Besides a validation study for the Smagorinsky and the WALE subgrid scale model, the resolution requirements of resolved LES are determined. Furthermore different subgrid filters are compared and the choice of the Smagorinsky constant in the case of filtered LES is briefly examined. [less ▲]

Detailed reference viewed: 226 (15 ULg)
Peer Reviewed
See detailDevelopment of a HPLC/UV-FLD method to detect the 15(+1) EU priority Polycyclic Aromatic Hydrocarbons (PAHs) in food supplements
Danyi, Sophie ULg; Brose, François ULg; Melens, Danielle ULg et al

in Book of abstracts - 3rd International Symposium on Recent Advances in Food Analysis (2007)

Detailed reference viewed: 73 (16 ULg)
Peer Reviewed
See detailDevelopment of a hydrogel containing GM-CSF to improve the dendritic cells colonisation of HPV+ epithelium
Evrard, Brigitte ULg; Hubert, Pascale ULg; Delvenne, Philippe ULg et al

in Proceedings of Eur conference on drug delivery and pharmaceutical technology (2004)

Detailed reference viewed: 21 (1 ULg)
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See detailDevelopment of a hydrogen maser in the TE111 mode
Van Der Beken, Emeline ULg; Léonard, Daniel; Counet, Arnaud et al

Poster (2016, May 18)

We present the recent progress in the development of a hydrogen maser in the unusual TE111 mode. In contrast to standard hydrogen maser that exploits the TE011 mode, the TE111 mode allows one to design ... [more ▼]

We present the recent progress in the development of a hydrogen maser in the unusual TE111 mode. In contrast to standard hydrogen maser that exploits the TE011 mode, the TE111 mode allows one to design hydrogen masers with significant reduced dimensions which represents a huge benefit for space applications and in particular for the global positioning system. We present in details the different parts of our model : cavity with a thin Teflon sheet, magnetic shielding, temperature control of the maser... [less ▲]

Detailed reference viewed: 37 (2 ULg)
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See detailDevelopment of a LC-MS/MS analytical method for the simultaneous measurement of aldehydes coming from polyunsaturated fatty acids degradation in animal feed
Douny, Caroline ULg; Bayram, Pinar; Brose, François ULg et al

in Drug Testing and Analysis (2016), 8(5-6), 458-464

Knowing that polyunsaturated fatty acids can lead to the formation of potentially toxic aldehydes as secondary oxidation products, an analytical method using liquid chromatography coupled to tandem mass ... [more ▼]

Knowing that polyunsaturated fatty acids can lead to the formation of potentially toxic aldehydes as secondary oxidation products, an analytical method using liquid chromatography coupled to tandem mass spectrometry detection (LC-MS/MS) has been developed to evaluate the concentration of eight aldehydes in animal feed: malondialdehyde (MDA), 4-hydroxy-2-nonenal (4-HNE), 4-hydroxy-2-hexenal (4-HHE), crotonaldehyde (CRT), benzaldehyde (BNZ), hexanal (HXL), 2,4-nonadienal and 2,4-decadienal. The developed method has been validated according to the criteria and procedure described in international standards. The evaluated parameters were: specificity/selectivity, recovery, precision, accuracy, uncertainty, limits of detection and quantification, using the concept of accuracy profiles. These parameters have been determined during experiments realized on 3 different days with grounded Kellogg’s® Corn Flakes® cereals as model matrix for animal feed and spiked at different levels of concentration. Malondialdehyde, 4-HHE, 4-HNE, crotonaldehyde, benzaldehyde and hexanal can be analysed in the same run in animal feed with a very good accuracy, with recovery rates ranging from 86 to 109% for a working range going from 0.16 to 12.50 mg/kg. Concerning 2,4-nonadienal and 2,4-decadienal, their analysis can be realized as well but in a limited range of concentration and with a limited accuracy. Indeed, recovery rates ranged between 54 and 114% and coefficient of variation for the intermediate precision between 11 and 25% for these two compounds. [less ▲]

Detailed reference viewed: 17 (2 ULg)
See detailDevelopment of a LC-UV-MS analytical method for malondialdehyde.
Douny, Caroline ULg; Dure, Remy; Brose, François ULg et al

Poster (2011)

Detailed reference viewed: 29 (3 ULg)