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See detailDetection of bone sialoprotein in human (pre)neoplastic lesions of the uterine cervix
Detry, Cédric ULg; Waltregny, David ULg; Quatresooz, Pascale ULg et al

in Calcified Tissue International (2003), 73(1), 9-14

Bone sialoprotein (BSP) is a secreted glycoprotein primarily found in the mineral compartment of developing bones. BSP is detected in a variety of human cancers, particularly those that metastasize to the ... [more ▼]

Bone sialoprotein (BSP) is a secreted glycoprotein primarily found in the mineral compartment of developing bones. BSP is detected in a variety of human cancers, particularly those that metastasize to the skeleton. High expression of BSP in breast and prostate primary carcinomas is associated with progression and bone metastases development. Since squamous cell carcinoma (SCCs) of the uterine cervix also frequently metastasizes to bone, we investigated whether BSP is expressed in human cervical cancer. We examined BSP expression in cervical tissue samples from 47 patients, including 19 normal tissues, 20 squamous intraepithelial lesions (SILs) (9 low and 11 high grade) and 8 invasive SCCs. BSP protein expression was evaluated by the immunophosphatase technique using a BSP polyclonal antibody in paraffin-embedded cervical biopsies. The abundance of BSP protein was significantly higher in invasive SCCs and high grade SILs than in normal cervix tissue samples and low grade SILs, which showed no or a low level of anti-BSP immunoreactivity. In situ hybridization experiments performed on representative cervix invasive SCCs frozen sections revealed that BSP transcripts were detectable in these lesions. Our study demonstrates that BSP expression is a common feature in high grade SILs and invasive SCCs of the uterine cervix. The prognostic value of BSP detection in these lesions and the potential role of BSP as an angiogenic factor in this type of cancer are currently under investigation. [less ▲]

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See detailDetection of Bone Sialoprotein in Human Breast Cancer Tissue and Cell Lines at Both Protein and Messenger Ribonucleic Acid Levels
Bellahcene, Akeila ULg; Antoine, Nadine ULg; Clausse, N. et al

in Laboratory Investigation : Journal of Technical Methods & Pathology (1996), 75(2), 203-10

The recent demonstration that bone sialoprotein (BSP) can be detected in human breast cancer tissue by immunoperoxidase suggests that this phosphoprotein is ectopically expressed by malignant mammary ... [more ▼]

The recent demonstration that bone sialoprotein (BSP) can be detected in human breast cancer tissue by immunoperoxidase suggests that this phosphoprotein is ectopically expressed by malignant mammary epithelial cells. Its detection in human breast cancer cells raises questions about its potential role(s) during breast cancer progression. Because BSP is secreted and is present in the serum, the positivity of breast cancer cells for BSP could have been the result of an uptake of the circulating phosphoprotein by the cells rather than of an intrinsic expression. We examined the expression of BSP at both the protein and mRNA levels in nine human breast cancer samples as well as in three human breast cancer cell lines (MCF-7, T47-D, and MDA-MB-231) using immunohistochemistry, flow cytometric analysis, immunoblot, and reverse-transcriptase PCR. BSP was detected at both protein and mRNA levels in human breast cancer tissue and in the three human breast cancer cell lines. Using a specific polyclonal anti-BSP antibody, we showed by both fluorescence-activated cell sorter analysis and immunohistochemistry experiments that all of the human breast cancer cell lines studied express BSP. This was localized at the cell surface and in the cytosol of the estrogen receptor-positive MCF-7 and T47-D cell lines, whereas it was detected only in the cytosol of the estrogen receptor-negative MDA-MB-231 cells. Using the same polyclonal anti-BSP antibody, we were able to identify an approximately 97-kd band on total protein extracts from the three cell lines by immunoblotting. Reverse-transcriptase PCR reactions using specific oligonucleotides performed on total RNA of nine human breast cancer biopsy samples and the three cell lines demonstrated the presence of BSP mRNA in all of the samples examined. This study is the first demonstration that human malignant breast epithelial cell lines express BSP at the protein and mRNA levels. Our study identified MCF-7, T47-D, and MDA-MB-231 cells as useful models for the examination of the molecular mechanisms involved in the ectopic expression of BSP in breast malignant lesions. [less ▲]

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See detailDetection of bovine leukaemia virus antibodies. Use of monoclonal antibody to increase sensitivity in an ELISA test.
Portetelle, Daniel ULg; Bruck, C.; Mammerickx, M. et al

in Archives Internationales de Physiologie et de Biochimie (1982), 90(3),

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See detailDetection of Bovine Leukemia Virus antibodies : use of monoclonal antibody to increase sensitivity and specificity of the ELISA test.
Portetelle, Daniel ULg; Bruck, Claudine; Mammerickx, Marc et al

in Straub, O. C. (Ed.) Proceedings of the Fifth International Symposium on bovine leukosis (1984)

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See detailDetection of bovine leukemia virus specific antibodies using recombinant p24-ELISA.
Gutierrez, G.; Alvarez, I.; Fondevila, N. et al

in Veterinary Microbiology (2009), 137(3-4), 224-34

We developed an indirect ELISA test (rp24-ELISA) for the detection of antibodies against the p24 capsid protein of bovine leukemia virus (BLV) in bovine serum samples. A recombinant p24 (rp24) was ... [more ▼]

We developed an indirect ELISA test (rp24-ELISA) for the detection of antibodies against the p24 capsid protein of bovine leukemia virus (BLV) in bovine serum samples. A recombinant p24 (rp24) was expressed in the Escherichia coli expression system, purified in a nickel charged resin and used as antigen in the ELISA test. A cut-off point was established considering the distribution of reactivity values obtained by rp24-ELISA on a set of 555 field serum samples that were stated as double positive (DP) or double negative (DN) by the combination of commercial agar gel immunodiffusion (AGID) assay and gp51-ELISA tests results. The rp24-ELISA showed good concordance with the agar gel immunodiffusion assay, when 710 serum samples were analyzed. In addition, rp24-ELISA demonstrated to be a precise assay with good repeatability and reproducibility and a better analytical sensitivity than AGID. From 67 discordant rp24-ELISA-AGID sera, 4 negative reactors were from the DP group, 28 positive samples were from the DN group and 35 positive samples were stated as negative by AGID but positive by the gp51-ELISA. Samples from this last subgroup were sent to an OIE reference laboratory where 28 samples were stated as negative, 5 samples were stated as positive and 2 as inconclusive. Complete concordance with blind previous results from an international proficiency test panel confirmed the capability of the assay to discriminate between infected and non-infected animals. In conclusion, the rp24-ELISA developed and standardized demonstrated to have good analytical characteristics to be considered for screening of BLV. [less ▲]

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See detailDetection of bright multiply imaged quasars by GAIA
Finet, François ULg

Scientific conference (2011, June 06)

Bright multiply imaged quasars offer unique tools to investigate the properties of the Universe as a whole (cosmological parameters), the astrophysical properties of the remote galaxies acting as ... [more ▼]

Bright multiply imaged quasars offer unique tools to investigate the properties of the Universe as a whole (cosmological parameters), the astrophysical properties of the remote galaxies acting as deflectors (i.e. their mass, extinction law, ...) as well as the structure of the background sources (accretion disk, central engine, etc.). Multiply imaged quasars will represent the most easily identified signature of lensing in GAIA observations. Several thousands of such cases are expected with J <=20. Furthermore, the complete QSO catalog which will be built from the GAIA observations will be very attractive and unique in order to study QSO evolution, large scale structures, intervening absorbers, the inertial frame, ... The aim of this presentation is to report about a realistic estimate of the expected number of bright multiply imaged quasars that will be detected by GAIA. [less ▲]

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See detailDetection of carbon stock change in agricultural soils using spectroscopic techniques
Stevens, Antoine; Van Wesemael, Bas; Vandenschrick, Grégoire et al

in Soil Science Society of America Journal (2006), 70(3, MAY-JUN), 844-850

Soil organic carbon (SOC) represents one of the major pools in the global C cycle. Therefore, even small changes in SOC stocks cause important CO, fluxes between terrestrial ecosystems and the atmosphere ... [more ▼]

Soil organic carbon (SOC) represents one of the major pools in the global C cycle. Therefore, even small changes in SOC stocks cause important CO, fluxes between terrestrial ecosystems and the atmosphere. However, SOC stocks are difficult to quantify accurately due to their high spatial variability. The aim of this paper is to evaluate the potential of Imaging Spectroscopy (IS) using the Compact Airborne Spectrographic Imager (CASI; 405-950 nm) and field spectroscopy with an Analytical Spectral Devices spectrometer (ASD; 350-2500 nm) to measure SOC content in heterogeneous agricultural soils. We used both stepwise and partial least square (PLS) regression analysis to relate spectral measurements to SOC contents. Standard Error of Prediction (SEP) for the ASD ranged from 2.4 to 3.3 g C kg(-1) depending on soil moisture content of the surface layer. Imaging spectroscopy performed poorly, mainly due to the narrow spectral range of the CASI. Tests using both the CASI and the Shortwave infrared Airborne Spectrographic Imager (SASI; 900-2500 nm) showed better results. The variation in soil texture and soil moisture content degrades the spectral response to SOC contents. Currently, SEP allows to detect a SOC stock change of 7.2-9.9 Mg C ha(-1) in the upper 30 cm of the soil, and is therefore still somewhat high in comparison with changes in SOC stocks as a result of management or land conversion (0.34.9 Mg C ha(-1) yr(-1)). A detailed SOC maps produced by IS reflected the patterns in SOC contents due to the recent conversion from grassland to cropland. [less ▲]

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See detailDetection of carbonyl fluoride in the stratosphere
Rinsland, C. P.; Zander, Rodolphe ULg; Brown, L. R. et al

in Geophysical Research Letters (1986), 13(8), 769--772

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See detailDetection of Chloroplast DNA by Using Fluorescent Monoclonal Anti-Bromodeoxyuridine Antibody and Analysis of Its Fate During Zygote Formation in Chlamydomonas Reinhardtii
Munaut, Carine ULg; Dombrowicz, D.; Matagne, René-Fernand ULg

in Current Genetics (1990), 18(3), 259-63

A monoclonal anti-bromodeoxyuridine antibody conjugated to fluorescein was used to detect the chloroplast nucleoids after specific incorporation of bromodeoxyuridine (BUdR) into the chloroplast DNA of ... [more ▼]

A monoclonal anti-bromodeoxyuridine antibody conjugated to fluorescein was used to detect the chloroplast nucleoids after specific incorporation of bromodeoxyuridine (BUdR) into the chloroplast DNA of Chlamydomonas reinhardtii. The incorporation of BUdR was enhanced by simultaneous treatment with fluorodeoxyuridine (FUdR). The method was applied to analyze the fate of chloroplast DNA in zygotes resulting from mating between BUdR-treated gametes (mt+ or mt-) and untreated gametes of opposite mating-type. In crosses between wild-type strains, the nucleoids of mt+ origin remained in the large majority of zygotes whereas those of mt- origin most often disappeared within the first hours following copulation. In crosses of the type mat-3 mt+ x wild-type mt- (the mat-3 mutation permits a high transmission of chloroplast genes from the mt- parent), the nucleoids of mt- origin were generally not eliminated which indicates that the mat-3 mutation prevents the selective destruction of paternal chloroplast DNA in the zygote. [less ▲]

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See detailDetection of Citrus Psorosis Virus Using an Improved One-Step RT-PCR
Achachi, Asmae; Jijakli, Haissam ULg; El Fahime, Elmostafa et al

in Arabian Journal for Science and Engineering (2014)

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See detailDetection of Clostridium botulinum neurotoxins A and B in milk by ELISA and immuno-PCR at higher sensitivity than mouse bio-assay
Rajkovic, Andreja; Elmoualij, Benaïssa ULg; Fikri, Youssef et al

in Food Analytical Methods (2011), [Epub ahead of print]

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See detailDetection of complement-dependent lytic antibodies in sera from bovine leukemia virus-infected animals.
Portetelle, Daniel ULg; Bruck, Claudine; Burny, Arsène et al

in Annales de Recherches Vétérinaires = Annals of Veterinary Research (1978), 9(4), 667-674

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See detailDetection of copy number variants in the horse genome and examination of their association with recurrent laryngeal neuropathy
Dupuis, Marie-Capucine; Zhang, Zhiyan ULg; Durkin, Keith ULg et al

in Animal Genetics (2013)

We used the data from a recently performed genome-wide association study using the Illumina Equine SNP50 beadchip for the detection of copy number variants (CNVs) and examined their association with ... [more ▼]

We used the data from a recently performed genome-wide association study using the Illumina Equine SNP50 beadchip for the detection of copy number variants (CNVs) and examined their association with recurrent laryngeal neuropathy (RLN), an important equine upper airway disease compromising performance. A total of 2797 CNVs were detected for 477 horses, covering 229 kb and seven SNPs on average. Overlapping CNVs were merged to define 478 CNV regions (CNVRs). CNVRs, particularly deletions, were shown to be significantly depleted in genes. Fifty-two of the 67 common CNVRs (frequency ! 1%) were validated by association mapping, Mendelian inheritance, and/or Mendelian inconsistencies. None of the 67 common CNVRs were significantly associated with RLN when accounting for multiple testing. However, a duplication on chromosome 10 was detected in 10 cases (representing three breeds) and two unphenotyped parents but in none of the controls. The duplication was embedded in an 8-Mb haplotype shared across breeds. [less ▲]

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See detailDetection of counterfeit Viagra by Raman microspectroscopy imaging and multivariate analysis
Sacré, Pierre-Yves ULg; Deconinck, Eric; Saerens, Lien et al

Conference (2011, May 13)

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See detailDetection of counterfeit Viagra® by Raman Microspectroscopy imaging and multivariate analysis.
Sacré, Pierre-Yves ULg; Deconinck, Eric; Saerens, Lien et al

in Journal of Pharmaceutical & Biomedical Analysis (2011), 56

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See detailDetection of Crustal Deformation on Mt. Etna using the ROB-IIV GPS Network
Bruyninx, Carine; Warnant, René ULg

Poster (1994)

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See detailDetection of Cytokines in Human Sural Nerve Biopsies: An Immunohistochemical and Molecular Study
Deprez, Manuel ULg; Lübke, U.; Verlaet, Myriam ULg et al

in Acta Neuropathologica (2001), 101(4), 393-404

In vitro and in vivo models have implicated numerous cytokines as major modulators of inflammation, destruction and repair in the peripheral nervous system (PNS). The in situ production of cytokines in ... [more ▼]

In vitro and in vivo models have implicated numerous cytokines as major modulators of inflammation, destruction and repair in the peripheral nervous system (PNS). The in situ production of cytokines in human peripheral nerve disorders is still poorly documented. We studied the expression of interleukin (IL)-1 beta, tumor necrosis factor (TNF)-alpha, IL-6, IL-10, IL-4, IL-3 and nerve growth factor (NGF) in 35 human sural nerve biopsies using immunohistochemistry; additional reverse transcription-polymerase chain reaction and mRNA in situ hybridization were performed for IL-4 and NGF. Expression of IL-1 beta and TNF-alpha was shown in both morphologically normal nerves and various neuropathies, and macrophages appeared as their predominant source. Levels of IL-1 beta and TNF-alpha expression were significantly correlated (P < 0.01) with each other and with expression of NGF. Multiple endoneurial sources were suggested for IL-6 and IL-10 with low immunoreactivity in the vast majority of cases. Conversely, IL-4 and IL-3 expression were found in neuropathies of various etiologies and Schwann cells appeared to be a predominant source of IL-4 in double-labeling immunofluorescence studies. IL-3 immunoreactivity correlated with IL-1 beta, TNF-alpha and IL-6. In this retrospective study, no specific cytokine profile of expression could be assigned to a precise subgroup of neuropathies. This is the first report of IL-4 and IL-3 expression in human neuropathies, and it may be important given the potential role of these cytokines in modulating macrophage activity in the PNS. [less ▲]

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