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See detailDevelopment and validation of analytical methods based on GC-MS/MC Triple Quadrupole instrument for the analysis of POPs in food and feed matrices
Calaprice, Chiara ULg

Doctoral thesis (2016)

This thesis focused on the development and validation of analytical methods for the de-tection and quantification of Persistent Organic Pollutants (POPs) in biological matrices, namely food and feed ... [more ▼]

This thesis focused on the development and validation of analytical methods for the de-tection and quantification of Persistent Organic Pollutants (POPs) in biological matrices, namely food and feed samples. POPs are a group of chemical compounds listed after the Stockholm Convention in 2001, with demonstrated toxicity and dangerousness for envi-ronment, animals and humans. In this work of thesis, special attention was reserved to some selected POPs: polychlo-rodibenzo-p-dioxins (PCDDs), polychlorodibenzofurans (PCDFs), usually referred to as “dioxins”, and polychlorinated biphenyls (PCBs), as there is a big concern about these contaminants in Taranto, a city in the Southern Italy very close to Bari, my home town. Taranto, indeed, is characterized by a large industrial area with a steel mill, several incin-erators and a refinery in few kilometre radius (Di Leo et al., 2014). This work has been done in collaboration with the University of Liège (Belgium) where a consolidated exper-tise in the field of POP measurements was available, especially in terms of dioxin anal-yses. In Chapter 1 the main steps, from sample preparation to data elaboration, of a validated confirmatory method for dioxin and PCB detection in food and feed using gas chromatog-raphy coupled to tandem mass spectrometry Triple Quad instrument (GC-MS/MS Triple Quad) have been described. This method was developed at the University of Liège in the framework of the last updates of the EU Regulation that in 2014 allowed confirmatory quantitative analysis of dioxins with Triple Quad. This method was the starting point of this work of thesis, because it was used for all dioxin and PCB quantifications. In Chapter 2 and chapter 3 alternative clean-up approaches for dioxin analysis in fatty food matrices have been developed using different automated systems. These works have been done in the framework of solvent and time saving for high throughput analytical methods in dioxin analysis. In chapter 2, an already existing automated system, DEXTechTM from LCTech GmbH (LCTech GmbH, Bahnweg 41, 84405 Dorfen, Germany) was used for sample clean-up, but a completely new clean-up approach was developed with this. GC-MS/MS Triple Quad instrument, as well as Magnetic Sector High Resolution Mass Spectrometry (HRMS) instrument were employed for final quantification, to demonstrate the suitability of our newly developed clean-up approaches whatever the instrumental detection. In Chapter 3 PowerPrepTM automated system from Fluid Management System (FMS Inc., 580 Pleasant Street, Watertown, MA 02472, USA) was used and our routine sample clean-up approach was modified in order to enhance the efficiency and to reduce cost and solvent consumption of the analysis. In Chapter 4 the main method for dioxin analysis was adapted for the integration of Dechloranes in the list of the analytes targeted in the regular control for dioxins in food and feed. Dechloranes are a family of 6 organo-chlorinated compounds with structure similar to Mirex, also called Dechlorane, a POP listed in the Stockholm convention. Dechloranes have been found in human blood of people from Europe (Brasseur et al., 2014) and in this work an analytical method for Dechlorane detection was developed and validated to investigate food as a possible route of exposure for humans in Europe, where no production plant has been identified so far. The analytical method was used to analyse 88 food and feed real sample and to give an idea of Dechlorane daily dietary intake. [less ▲]

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See detailDevelopment and validation of analytical methods for quantifying isoflavones and equol in biological matrices
Daems, Frédéric ULg

Doctoral thesis (2016)

Isoflavones are natural plant substances that attract widespread interest because of their association with a large variety of beneficial effects on human health. When they are ingested by animals or ... [more ▼]

Isoflavones are natural plant substances that attract widespread interest because of their association with a large variety of beneficial effects on human health. When they are ingested by animals or humans, most of these compounds are metabolized by bacteria present in the digestive system. Among the isoflavone microbial metabolites, equol is probably the most widely studied because it seems to have numerous health benefits. In the human population, however, not everyone is able to produce equol. Equol enrichment of some food, such as cow's milk, could be an alternative strategy for making equol’s health benefits available to everyone. The main objective of the present thesis was to develop analytical tools for quantifying these compounds in several biological matrices, thus contributing to the feasibility study being conducted on producing milk naturally enriched with equol. For this purpose, four analytical procedures, using ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC®-MS/MS), were developed and fully validated. The analytical tools were then applied successfully in two original exploratory studies that sought to quantify equol concentrations in commercial cow's milk in Belgium and study the evolution of isoflavone concentration in red clover (Trifolium pratense L.) silage in laboratory-scale silos. This thesis describes new and reliable analytical tools that will help to improve current knowledge about the feasibility of producing cow's milk naturally enriched with equol and to study isoflavone metabolization in dairy cow. [less ▲]

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See detailDevelopment and Validation of LC Method for the Diclofenac Sodium Release Determination Using Accuracy Profile Concept
Al-Kamarany, M.A.; El Karbane, M; Belomaria, M et al

in Asian Journal of Chemistry (2012), 24(10), 4393-4400

The article presents a rapid and sensitive RP-HPLC method with UV detection, using the diclofenac sodium release determination in solid pharmaceutical formulations. A novel validation strategy based on ... [more ▼]

The article presents a rapid and sensitive RP-HPLC method with UV detection, using the diclofenac sodium release determination in solid pharmaceutical formulations. A novel validation strategy based on accuracy profiles was used to select the most appropriate regression model with highest accuracy within well defined acceptance limits. Furthermore, the strategy was used to determine the limits of quantification as well as the suitable concentration range. The validation phase was completed by investigating of the risk profiles of various acceptable regression models in order to avoid obtaining measurements outside the acceptance limits fixed a priori. On the other hand, the present study shows how the LC method can be used more accurately to assess the kinetic dissolution profiles, instead the UV-visible method required by monographs of the USP. Robustness study was also performed in order to demonstrate the capability of this method to remain unaffected by a small and deliberate variation in method parameters. The LC method was validated using the total error approach, as a decision tool, guarantees that each of the future results that will be within the acceptance limits settled at ± 5 %. The UV spectrophotometric method based on the USP monograph, gives rise of impurities from diclofenac sodium in acidic condition (HCl 0.1 N). These impurities absorb at the same wave length (276 nm) as the active principal ingredient, which will yield some significant error in the per cent release during the dissolution test study. Described analytical method is a simple, sensitive, specific and more accurate indicating that this LC method is useful for manufacturing and quality control assay. [less ▲]

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See detailThe development and validation of nursing related groups based on the Belgian Nursing Minimum Dataset
THONON, Olivier ULg; VAN HERCK, Pieter; GILLAIN, Daniel ULg et al

in SHEERIN, Fintan; SERMEUS, Walter; EHRENBERG, Anna (Eds.) ACENDIO 2013 - Proceedings of 9th European Conference of ACENDIO (2013, March)

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See detailDevelopment and validation of nursing resource weights for the Belgian Nursing Minimum Dataset in general hospitals: a Delphi questionnaire survey approach.
SERMEUS, Walter; GILLET, Pierre ULg; GILLAIN, Daniel ULg et al

in International Journal of Nursing Studies (2009), 46(2), 256-67

BACKGROUND: Internationally, nursing is not well represented in hospital financing systems. In Belgium a nursing weight system exists to adjust budget allocation for differences in nurse staffing ... [more ▼]

BACKGROUND: Internationally, nursing is not well represented in hospital financing systems. In Belgium a nursing weight system exists to adjust budget allocation for differences in nurse staffing requirements, but there is a need for revision. Arguments include the availability of a nursing minimum dataset and the adverse consequences of the current historically based nursing weight system. OBJECTIVES: The development and validation of nursing resource weights for the revised Belgium nursing minimum dataset (NMDS). DESIGN: Two independent cross sectional Delphi-surveys. SETTING AND PARTICIPANTS: A convenience sample of 222 head nurses from 69 Belgian hospitals participated in the cross sectional survey methods. To assess validity 112 patient case records from 61 nursing wards of 35 Belgian general hospitals representing general, surgical, pediatric, geriatric and intensive care were selected. METHODS: Nursing resource weights were constructed based on Delphi survey results by NMDSII intervention. The patient case Delphi survey results were used as the primary source for validation. A series of additional validation measures were calculated, based on the different patient classification systems. Finally, three validated nursing resource weighting systems were compared to the constructed NMDSII weighting system: the use of 'Closon', 'Ghent' and WIN weights. RESULTS: A coherent set of nursing resource weights was developed. The comparison of nurse resource weights, based on the survey per NMDS intervention versus the survey on patient cases, yielded high correlations: r=0.74 to r=0.97 (p<0.01) between three case rating questions, as an indication of reliability in terms of internal consistency, and r=0.90 (p<0.01) between summed intervention weights and patient case weights, as an indication of criterion validity in terms of concurrent validity. Other concurrent validity measures based on summed intervention weights versus patient classification dependency weights showed a correlation ranging from r=0.14 to r=0.74. The correlation of summed intervention weights with the Closon, Ghent and WIN weights ranged from r=0.93 to r=0.96 (p<0.01), as a third indication of concurrent validity. CONCLUSIONS: A system of valid nursing resource weights has been developed. The system should be further validated within an international context. [less ▲]

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See detailDevelopment and validation of situational judgment tests for evaluating Organizational Citizenship Behavior and Contextual Performance Behavior in a professional context : methodological issues.
Lothe, Benoit ULg; Hansez, Isabelle ULg

Conference (2011, June 22)

Strategic Human Resource Management typically raises appraisal issues (Analoui, 2007; Jackson, Schuler & Werner, 2009 ; Teo & Crawford, 2005) mostly related to the HRM relationship with organizational ... [more ▼]

Strategic Human Resource Management typically raises appraisal issues (Analoui, 2007; Jackson, Schuler & Werner, 2009 ; Teo & Crawford, 2005) mostly related to the HRM relationship with organizational performance (Bowen & Ostroff, 2004 ; Cascio, 1996 ; Wright, Gardner, Moynihan & Allen, 2005). Hence workforce assessment remains a key success factor for many organizations. Thus this paper takes place in the early beginning of a PhD Thesis which aims to the development and validation of an assessment test of Organizational Citizenship Behavior (OCB) and Contextual Performance (CP) in a professional selection context. The managerial concept of OCB refers to “individual behavior that is discretionary, not directly or explicitly recognized by the formal reward system, and in the aggregate promotes the efficient and effective functioning of the organization” (Organ, Podsakoff & MacKenzie, 2006, p. 3). CP is a closely related construct, distinct of task performance, that encompasses behaviors supporting the psychological and social context in which task activities are performed (Stone-Romero, Alvarez, & Thompson, 2009). If these two frameworks look much alike, they contrast on several points: conceptual dimensions, discretional characteristics of behavior, etc. Therefore, it seems interesting to apprehend both commonly. Situational Judgment Tests (SJTs) show a good validity concerning prediction of job performance in employment settings (Lievens, Peeters, Schollaert, 2008 ; Whetzel & McDaniel, 2009). Furthermore, they are a multidimentional measurement method suited to assess different construct like job knowlege and skills, applied social skills, and some basic personality tendencies (Christian, Edwards, & Bradley, 2010). Taking into account its characteristics, SJTs seem to be an appropriate method to enable OCB & CP evaluation. This communication pursues three goals. The first is a theoretical issue: we need a straightforward vision of OCB & CP concepts. Accordingly, after a consistent review on the vast amount of work that have been written on these two concepts, we have to specify what we seek to appraise and make the inventory of existing measurement tools related to these focus. We have already worked out a structured methodology to develop and validate SJTs in selection and career management context (Lothe, Hansez & Bertrand, Submitted). So the second issue is a methodological one. How to improve and/or to adapt this methodology in order to create a new SJT measuring OCB & CP ? Thirdly, some research related issues conclude this paper. Through this PhD project, we should be able to examine several main issues that will shape the thesis. Are SJTs being able to provide a valid assessment of OCB & CP in a personnel selection context ? Does the discretionary nature of one behavior impact the quality of its measurement ? What is the effect of response instructions on OCB/CP situational judgment test performance ? Each issue will be illustrated and discussed in connection with the development and validation opportunities and constraints. [less ▲]

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See detailDevelopment and validation of stable reference materials for food microbiology using Bacillus cereus and Clostridium perfringens spores
Abdelmassih, Marleen; Planchon, Viviane; Anceau, Christine ULg et al

in Journal of Applied Microbiology (2011)

To develop a new type of microbiological Reference Materials (RMs), displaying long-term stability at room temperature. The purpose was to produce and validate two batches of RMs for the enumeration of ... [more ▼]

To develop a new type of microbiological Reference Materials (RMs), displaying long-term stability at room temperature. The purpose was to produce and validate two batches of RMs for the enumeration of Bacillus cereus and Clostridium perfringens. Methods and Results: The RMs were based on spores of B. cereus and Cl. perfringens, adsorbed on calcium carbonate pellets. Two batches of 1000 units were manufactured and validated in compliance with ISO guide 35. After verification of their homogeneity, the stability of the ‘RM-B. cereus’ and ‘RMCl. perfringens’ batches was proven during at least 36 and 9 months, respectively, at room temperature. The validation study was completed by international collaborative trial involving 12 laboratories, allowing the validation of the assigned values. Conclusions: The methodology developed in this work enabled to produce easy-to-handle and cost-effective RMs, displaying an unprecedented stability at room temperature, a good homogeneity and a precise and validated assigned value. Significance and Impact of the Study: This study revealed new paths for the development of stable microbiological RMs. Overcoming the intrinsic instability of the living cells makes it possible to produce valuable tools for the quality assurance of microbiology laboratories. Jou [less ▲]

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See detailDevelopment and validation of the French version of a tool assessing patient's expectations in lower limb osteoarthritis
NEUPREZ, Audrey ULg; Delcour, Jean-Pierre; Fatemi, Firouzeh et al

in Journal of Orthopaedics (2015), 12

Objective: The Hospital for Special Surgery (HSS) Hip Replacement Expectations Survey and <br />Knee Replacement Expectations Survey are validated tools developed to measure patients' <br />preoperative ... [more ▼]

Objective: The Hospital for Special Surgery (HSS) Hip Replacement Expectations Survey and <br />Knee Replacement Expectations Survey are validated tools developed to measure patients' <br />preoperative expectations for hip and knee arthroplasty. These instruments have possible <br />uses in both daily practice and research. Our objective was to assess the test-retest reliability <br />and the construct validity of the French version of the surveys. <br />Methods: Patients scheduled for total hip (n ¼ 82) or knee replacement (n ¼ 61) aged 38e90 <br />years were included. All completed the HSS Hip or Knee Replacement Expectations Survey <br />and the Expectation WOMAC to determine concurrent validity. <br />The test-retest reliability was assessed using the intraclass coefficient correlation (ICC), <br />the Bland and Altman Method and the coefficient of variation; the internal consistency was <br />assessed by the Cronbach a coefficient. The construct validity was investigated using the <br />Pearson correlation coefficient and floor and ceiling effects by percentage frequency of <br />lowest or highest possible score achieved by respondents. <br />Results: 143 patients scheduled for hip or knee arthroplasty were included. <br />The reliability was excellent between the test and the rested total score, with an ICC of <br />0.902 (0.853e0.936) and CV of 4.06% for the French Hip Replacement Expectations Survey <br />and 0.865 (0.786e0.917) and CV of 7.7% for the French Knee Replacement Expectations <br />Survey, without bias. <br />The Cronbach a coefficient was 0.72 for hip Survey and 0.82 for knee Survey showing a <br />good internal consistency. <br />Pearson correlation coefficients of 0.45 and 0.48 between Expectations WOMAC and <br />HSS, respectively for hip Survey and knee Survey, were observed but with systematic bias. <br />The lowest possible score was not reported by any patient and only three patients <br />(3.66%) scheduled for hip arthroplasty reported the highest possible score. Conclusions: The French version of the HSS Hip or Knee Replacement Expectations Survey is <br />a reliable and valid questionnaire and compares favourably with the original English <br />version. Therefore, this new version may help French-speaking clinicians to evaluate expectations <br />before lower limb arthroplasty. [less ▲]

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See detailDevelopment and validation of the ORACLE score to predict risk of osteoporosis
Richy, F.; Deceulaer, F.; Ethgen, Olivier ULg et al

in Mayo Clinic Proceedings (2004), 79(11), 1402-1408

OBJECTIVE: To develop and validate a composite index, the Osteoporosis Risk Assessment by Composite Linear Estimate (ORACLE), that includes risk factors and ultrasonometric outcomes to screen for ... [more ▼]

OBJECTIVE: To develop and validate a composite index, the Osteoporosis Risk Assessment by Composite Linear Estimate (ORACLE), that includes risk factors and ultrasonometric outcomes to screen for osteoporosis. SUBJECTS AND METHODS: Two cohorts of postmenopausal women aged 45 years and older, participated in the development (n = 407) and the validation (n = 202) of ORACLE. Their bone mineral density was determined by dual energy x-ray absorptiometry and quantitative ultrasonometry (QUS), and their historical and clinical risk factors were assessed (January to June 2003). Logistic regression analysis was used to select significant predictors of bone mineral density, whereas receiver operating characteristic (ROC) analysis was used to assess the discriminatory performance of ORACLE. RESULTS: The final logistic regression model retained 4 biometric or historical variables and 1 ultrasonometric outcome. The ROC areas under the curves (AUCs) for ORACLE were 84% for the prediction of osteoporosis and 78% for low bone mass. A sensitivity of 90% corresponded to a specificity of 50% for identification of women at risk of developing osteoporosis. The corresponding positive and negative predictive values were 86% and 54%, respectively, in the development cohort. In the validation cohort, the AUCs for identification of osteoporosis and low bone mass were 81% and 76% for ORACLE, 69% and 64% for QUS T score, 71% and 68% for QUS ultrasonometric bone profile index, and 76% and 75% for Osteoporosis Self-assessment Tool, respectively. ORACLE had the best discriminatory performance in identifying osteoporosis compared with the other approaches (P < .05). CONCLUSION: ORACLE exhibited the highest discriminatory properties compared with ultrasonography alone or other previously validated risk indices. It may be helpful to enhance the predictive value of QUS. [less ▲]

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See detailDevelopment and validation of the working memory self-assessment scale
Fresson, Megan ULg; DEMOULIN, Valentine ULg; HIERNAUX, Anne ULg et al

Poster (2013, July)

Aim. Because working memory is involved in many daily life activities, its ecological evaluation is a key dimension of the neuropsychological assessment of people with cognitive impairments. The aim of ... [more ▼]

Aim. Because working memory is involved in many daily life activities, its ecological evaluation is a key dimension of the neuropsychological assessment of people with cognitive impairments. The aim of this study was to develop and validate a 30-items self-assessment scale of working memory, the WMSS (Working Memory Self-Assessment Scale). Method. The WMSS and a comprehensive assessment battery of working memory and executive functions were administered to sixty French-speaking individuals (20 young, 20 old, 20 old-old). Results. The internal validity of the scale was strong as estimated by the Cronbach’s alpha coefficient (α = .93). Concerning the external validity, several correlations were obtained between the WMSS and the cognitive composite scores. Unlike old subjects, the lower cognitive results young and old-old subjects had, the more working memory complaints they expressed. Discussion. The WMSS shows a satisfactory internal as well as external validity since young and old-old subjects who reported more difficulties (WMSS) are those who obtained lower cognitive results. The somewhat surprising relationship between the WMSS and the cognitive tasks in the old group can be explained by an increased perception of cognitive changes and a more complex life style in old subjects with better cognitive abilities. [less ▲]

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See detailDevelopment by the sol–gel process of highly dispersed Ni–Cu/SiO2 xerogel catalysts for selective 1,2-dichloroethane hydrodechlorination into ethylene
Pirard, Sophie ULg; Mahy, Julien ULg; Pirard, Jean-Paul ULg et al

in Microporous and Mesoporous Materials (2015), 209

Ni–Cu/SiO2 xerogel catalysts have been synthesized by cogelation of industrial tetraethoxysilane (Dynasil) and chelates of Ni and Cu with industrial 3-(2-aminoethylamino)propyltrimethoxysilane (Dynasylan ... [more ▼]

Ni–Cu/SiO2 xerogel catalysts have been synthesized by cogelation of industrial tetraethoxysilane (Dynasil) and chelates of Ni and Cu with industrial 3-(2-aminoethylamino)propyltrimethoxysilane (Dynasylan DAMO) in industrial ethanol denatured with diethyl phthalate. Despite the use of industrial grade reagents, highly dispersed bimetallic Ni–Cu/SiO2 xerogel catalysts were obtained. These samples are composed of completely accessible Ni–Cu alloy crystallites with sizes of 1.6–3.4 nm located inside silica particles exhibiting a monodisperse microporous distribution. It appears that the bimetallic complex acts as a nucleation agent in the formation of silica particles. The combination of results obtained from the calculation of the metal ratio in catalysts, H2 chemisorption and transmission electron microscopy allowed calculating the surface composition of the nickel–copper particles in Ni–Cu/SiO2 cogelled xerogel catalysts. Values obtained indicate a very pronounced surface enrichment with copper. While 1,2-dichloroethane hydrodechlorination over pure nickel mainly produces ethane, increasing copper content in bimetallic catalysts results in an increase in ethylene selectivity. The specific consumption rate of 1,2-dichloroethane decreases when copper loading increases. The turnover frequency, that is, the number of catalytic cycle per active site (nickel atom and its surrounding copper atoms) and per second, seems to be independent of surface composition of alloy particles. [less ▲]

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See detailthe development care WEECARE reduces the stress in neonates
Battisti, Oreste ULg; NYAMUGABO MUNYERE NKANA, Kindja ULg; zigabe, serge et al

in Annales Africaine de Médecine (2015)

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See detailDevelopment Factors and Local Impacts of Agritourism: Comparison Between Slovenia and Walloon Region
Schmitz, Serge ULg; PotočnikSlavič, Irma

in Dubois, Charline (Ed.) Agritourism between embeddedness and internationalization (2012, June)

The bilateral research project between the Republic of Slovenia and Wallonie Bruxelles Internationale“Development Factors of Agritourism: Comparison Between Slovenia and Walloon Region” (L´agrotourisme ... [more ▼]

The bilateral research project between the Republic of Slovenia and Wallonie Bruxelles Internationale“Development Factors of Agritourism: Comparison Between Slovenia and Walloon Region” (L´agrotourisme, son fonctionnement et son rôle dans le développement local en Wallonie et Slovénie aujourd´hui et demain; Dejavnikirazvojaturizmanakmetiji: primerjava med Slovenijo in Valonijo; 2010-2012) was focused on agritourism in order to illustrate how sustainable development thinking can be applied when addressing the problems of the rural areas. It aimed as well to rethink agritourism development according to its embeddeness in order to enlighten the question which form of agritourism is suitable to which rural area and vice-versa. At the same time, agritourism is shaping and is being re-shaped by the internationalization of tourism market. [less ▲]

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See detailDevelopment in Red River Delta, Vietnam
Nguyen Thi Minh Khué, ULg; Nguyen Thi, Dien; White, Ben et al

Poster (2015)

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See detailDevelopment of Space Weather related services for real-time GPS applications
Warnant, René ULg; Bavier, Michaël; Lejeune, Sandrine et al

Conference (2005)

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See detaildevelopment of 4H-1,2,4-benzothiadiazine 1,1-dioxides as putative tocolytic agents: pharmacological evaluation on diferent smooth muscle preparations
Boverie, S.; Becker, B.; Sebille, S. et al

in Fundamental & Clinical Pharmacology (2002)

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See detailDevelopment of 4H-1,2,4-benzothiadiazine 1,1-dioxides as putative tocolytic agents: pharmacological evaluation on different smooth muscle preparations
Boverie, S.; Becker, B.; Sebille, S. et al

Poster (2002, March 02)

Detailed reference viewed: 4 (0 ULg)