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See detailEarly pregnancy diagnosis in sheep by progesterone and pregnancy-associated glycoprotein tests
Karen, A.; Beckers, Jean-François ULg; Sulon, Joseph ULg et al

in Theriogenology (2003), 59(9), 1941-1948

The aim of this study was to compare the accuracy of the progesterone (P4) and pregnancy associated glycoprotein (PAG) tests for determination of early pregnancy in sheep. Estrus was synchronized in 182 ... [more ▼]

The aim of this study was to compare the accuracy of the progesterone (P4) and pregnancy associated glycoprotein (PAG) tests for determination of early pregnancy in sheep. Estrus was synchronized in 182 Awassi x Merino ewes and blood samples were collected at Days 0 (day of the insemination), 18, 22, 29, 36, and 50 after artificial insemination (AI). Plasma P4 concentrations at Days 0 and 18 were determined by double antibody radioimmunoassay, while PAG concentrations at Days 22, 29, 36 and 50 were determined by a heterologous, double-antibody radioimmunoassay (RIA) using the bovine PAG 67 kDa subunit as tracer and standard and rabbit antiserum raised against a mixture of caprine 55 and 59 kDa PAG subunits as the first antibody. The discriminatory value for diagnosis of pregnancy by the P4 and the PAG-RIA tests was greater than or equal to1 ng/ml. Based on lambing data, the accuracy for diagnosing pregnant (sensitivity) and non-pregnant ewes (specificity) and predictivity of both tests were calculated. The sensitivity, specificity, positive and negative predictive values for P4 and PAG tests were 100, 95.4, 81.6, and 100% at Day 18 (P4) and 93.5, 100, 100 and 98.7% at Day 22 (PAG), respectively. For diagnosis of non-pregnant ewes the PAG test had significantly higher specificity than the P4 test (P < 0.05). It is concluded that ovine pregnancy can be reliably diagnosed at Day 22 after AI by using a heterologous radioimmunoassay of PAG. [less ▲]

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See detailEarly pregnancy diagnosis in sheep by progesterone and pregnancy-associated glycoprotein tests.
Karen, A.; Beckers, Jean-François ULg; Sulon, J. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 8

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See detailEarly prepubertal ontogeny of pulsatile gonadotropin-releasing hormone (GnRH) secretion: I. Inhibitory autofeedback control through prolyl endopeptidase degradation of GnRH.
Yamanaka, C.; LEBRETHON, Marie-Christine ULg; Vandersmissen, E. et al

in Endocrinology (1999), 140(10), 4609-15

GnRH[1-5], a subproduct resulting from degradation of GnRH by prolyl endopeptidase (PEP) and endopeptidase 24.15 (EP24.15) was known to account for an inhibitory autofeedback of GnRH secretion through an ... [more ▼]

GnRH[1-5], a subproduct resulting from degradation of GnRH by prolyl endopeptidase (PEP) and endopeptidase 24.15 (EP24.15) was known to account for an inhibitory autofeedback of GnRH secretion through an effect at the N-methyl-D-aspartate (NMDA) receptors. This study aimed at determining the possible role of such a mechanism in the early developmental changes in frequency of pulsatile GnRH secretion. Using retrochiasmatic explants from fetal male rats (day 20-21 of gestation), no GnRH pulses could be observed in vitro, whereas pulses occurred at a mean interval of 86 min from the day of birth onwards. This interval decreased steadily until day 25 (39 min), during the period preceding the onset of puberty. Based on GnRH[1-10] or GnRH[1-9] degradation and GnRH[1-5] generation after incubation with hypothalamic extracts, EP24.15 activity did not change with age, whereas PEP activity was maximal at days 5-10 and decreased subsequently until day 50. These changes were consistent with the ontogenetic variations in PEP messenger RNAs (mRNAs) quantitated using RT-PCR. Using fetal explants, the NMDA-evoked release of GnRH was potentiated in a dose-dependent manner by bacitracin, a competitive PEP inhibitor and the desensitization to the NMDA effect was prevented using 2 mM of bacitracin. At day 5, a higher bacitracin concentration of 20 mM was required for a similar effect. Pulsatile GnRH secretion from fetal explants was not caused to occur using bacitracin or Fmoc-Prolyl-Pyrrolidine-2-nitrile (Fmoc-Pro-PyrrCN), a noncompetitive PEP inhibitor. At postnatal days 5 and 15, a significant acceleration of pulsatility was obtained using 1 microM of Fmoc-Pro-PyrrCN or 2 mM of bacitracin. At 25 and 50 days, a lower bacitracin concentration of 20 microM was effective as well in increasing the frequency of GnRH pulsatility. We conclude that the GnRH inhibitory autofeedback resulting from degradation of the peptide is operational in the fetal hypothalamus but does not explain the absence of pulsatile GnRH secretion at that early age. After birth, PEP activity is high and may account for the low frequency of pulsatility. The potency of that effect decreases before the onset of puberty and may contribute to the acceleration of GnRH pulsatility. [less ▲]

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See detailEarly prognostic index and late exercise test comapred in survivors from acute myocardial infarction
Chapelle, Jean-Paul ULg; Smeets, J. P.; El Allaf, M. et al

Poster (1987, September 05)

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See detailEarly pronostic index and late exercise test compared in survivors from acute myocardial infarction
Chapelle, Jean-Paul ULg; Smeets, J. P.; El Allaf, M. et al

in European Heart Journal Supplements : Journal of the European Society of Cardiology (1987), 8(suppl.2), 11

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See detailEarly reactions of light-induced protochlorophyllide and chlorophyllide transformations analyzed in vivo at room temperature with a diode array spectrofluorometer
Boddi, B.; Popovic, R.; Franck, Fabrice ULg

in Journal of Photochemistry and Photobiology B : Biology (2003), 69(1), 31-39

The steps of protochlorophyllide (Pchlide) photoreduction and subsequent chlorophyllide (Chlide) transformations which occur in the seconds to minutes time-scale were studied using a diode array ... [more ▼]

The steps of protochlorophyllide (Pchlide) photoreduction and subsequent chlorophyllide (Chlide) transformations which occur in the seconds to minutes time-scale were studied using a diode array spectrofluorometer in dark-grown barley leaves. The intensity of the excitation light was varied between 3 and 2500 mumol m(-2) s(-1) and a series of fluorescence spectra were recorded at room temperature in the seconds and minutes time scales. In certain experiments, 77-K emission spectra were measured with the same equipment. The high quality of the spectra allowed us to run spectral resolution studies which proved the occurrence, at room temperature, of multiple Pchlide and Chlide forms found previously in 77-K spectra. The comparison of the 77-K and room-temperature spectra showed that the fluorescence yields of the nonphotoactive 633-nm Pchlide form and of the Chlide product emitting at 678 nm were temperature independent. The fluorescence intensity of aggregated NADPH-pigment-POR complexes (photoactive 656-nm Pchlide and 693-nm Chlide forms) were strongly increased at 77 K, while that of the NADP(+)-Chlide-POR (684-686-nm Chlide form) was much less affected by temperature. Information was obtained also about the dynamics of the transformation of pigment forms in the light at different photon densities. At low light intensities, the phototransformation of the 642-644-nm Pchlide form was faster than that of the 654-656-nm form. The relative amplitudes of Gaussian components related to different Chlide forms found after exposure to a constant amount of photons strongly depended on the light intensity used. Strong quenching of all Chlide components occurred upon prolonged exposure to high intensity light. These effects are discussed by considering the interconversion processes between different forms of the pigment-protein complexes, their relative fluorescence yields and energy migration processes. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailEarly release of myeloperoxydase and lactoferin after direct stenting in patient presented for unstable angina
Gach, Olivier ULg; Biemar, C.; Pierard, Luc ULg et al

in European Heart Journal (2004), 25(Suppl. S), 450-450

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See detailEarly release of neutrophil markers of activation after direct stenting in patients with unstable angina
Gach, Olivier ULg; Biemar, Christian; Nys, Monique ULg et al

in Coronary Artery Disease (2005), 16(1), 59-65

Objective To assess polymorphonuclear neutrophils activation after stenting in acute coronary syndromes studied by myeloperoxydase, lactoferrin and elastase release in this clinical setting. Methods ... [more ▼]

Objective To assess polymorphonuclear neutrophils activation after stenting in acute coronary syndromes studied by myeloperoxydase, lactoferrin and elastase release in this clinical setting. Methods Myeloperoxydase, lactoferrin, elastase, C-reactive protein and cytokines serum levels were assessed in 20 patients undergoing catheterization for unstable angina. Serial sampling starting before arteriography and continued up to 24 h was carried out in 15 patients undergoing direct stenting (group A) and in five patients assessed by coronary angiography only (group B). Results Myeloperoxydase, lactoferrin and elastase levels remained unchanged following catheterization, whereas a significant increase in myeloperoxydase (P=0.0009) and lactoferrin (P=0.004) was observed after stenting. No change in levels of tumour necrosis factor alpha, interleukin (IL)-8 and IL-12 was found in group B after catheterization at the different sampling times, although IL-8 and IL-12 levels increased transiently following stenting. IL-6 values increased in both groups. Baseline values of C-reactive protein were similar in each group. A progressive increase in C-reactive protein was noted in both groups and appeared to be larger following stenting (group A: P=0.0002; group B: P=0.01). Conclusions In patients with unstable angina, stenting is associated by immediate neutrophil activation followed by release of inflammatory cytokines (IL-6, IL-8, IL-12) and C-reactive protein elevation. This study points out a potential role of myeloperoxydase as a trigger for inflammatory reaction in patients with unstable coronary syndromes undergoing percutaneous coronary intervention. (C) 2005 Lippincott Williams WillZins. [less ▲]

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See detailEarly response of lymphocyte proteins after gamma-radiation
Turtoi, Andrei ULg; Srivastava, Alok; Sharan, Rajesh et al

in Journal of Radioanalytical & Nuclear Chemistry (2007), 274(2), 435-39

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See detailEarly results from the MAVEN mission and the Imaging UltraViolet Spectrograph
Schneider; Stiepen, Arnaud ULg; et al

Scientific conference (2015, March 30)

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See detailEarly retention in kindergarten: risk factors and pathways in primary education
Lafontaine, Dominique ULg; Chenu, Florent ULg; Dupont, Virginie

Conference (2013, August 31)

In French-speaking Belgium, about 4% of the children are retained in kindergarten. What are the pupils’ characteristics and the school context variables predictive of the retention in kindergarten? What ... [more ▼]

In French-speaking Belgium, about 4% of the children are retained in kindergarten. What are the pupils’ characteristics and the school context variables predictive of the retention in kindergarten? What are the pathways in primary education of “retained” pupils compared to their peers who started 1st grade “on time”? How can this early retention be explained? A broad database with entire cohorts has been used for studying the risk factors and the pupils’ school careers in primary education. Moreover, teachers’ beliefs about retention, learning and teaching have been investigated through a questionnaire administered to a representative sample of preschool and 1st grade teachers. The multilevel regression analyses show that the following variables are significantly predictive of retention in kindergarten: month of birth, gender, socioeconomic status, country of birth and at the school level, mean school social intake. Investigation about teachers’ belief shows that the vast majority of teachers are convinced that retention in kindergarten is beneficial for later achievement and not detrimental at a psychological level. Preschool and 1st grade teachers share some mutual expectations about the level of prerequisites for entering 1st grade. Even if they are no formal instructions or benchmarks, kids who are not “ready” should not enter 1st grade, but rather stay one more year in kindergarten. Those results can be related to the “culture” of the education system in Belgium, a non-comprehensive one: pupils with learning difficulties are kept apart from their age group or peers through grade retention, early tracking and special education. [less ▲]

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See detailEarly retention in kindergarten: risk factors and school career in French-speaking Belgium primary education
Lafontaine, Dominique ULg; Chenu, Florent ULg; Dupont, Virginie ULg

Conference (2013, August 31)

In French-speaking Belgium, about 4% of the children are retained in kindergarten. What are the pupils’ characteristics and the school context variables predictive of the retention in kindergarten? What ... [more ▼]

In French-speaking Belgium, about 4% of the children are retained in kindergarten. What are the pupils’ characteristics and the school context variables predictive of the retention in kindergarten? What are the pathways in primary education of “retained” pupils compared to their peers who started 1st grade “on time”? How can this early retention be explained? A broad database with entire cohorts has been used for studying the risk factors and the pupils’ school careers in primary education. Moreover, teachers’ beliefs about retention, learning and teaching have been investigated through a questionnaire administered to a representative sample of preschool and 1st grade teachers. The multilevel regression analyses show that the following variables are significantly predictive of retention in kindergarten: month of birth, gender, socioeconomic status, country of birth and at the school level, mean school social intake. Investigation about teachers’ belief shows that the vast majority of teachers are convinced that retention in kindergarten is beneficial for later achievement and not detrimental at a psychological level. Preschool and 1st grade teachers share some mutual expectations about the level of prerequisites for entering 1st grade. Even if they are no formal instructions or benchmarks, kids who are not “ready” should not enter 1st grade, but rather stay one more year in kindergarten. Those results can be related to the “culture” of the education system in Belgium, a non-comprehensive one: pupils with learning difficulties are kept apart from their age group or peers through grade retention, early tracking and special education. [less ▲]

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See detailEarly retirement in Europe: A call for action
Jousten, Alain ULg

in CESIFO Forum (2001), 4

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See detailEarly retirement, activities after retirement and the cognitive reserve
Adam, Stéphane ULg; Bay, Christelle; Perelman, Sergio ULg

Conference (2005, September 26)

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See detailEarly seed plant radiation : an ecological hypothesis.
Prestianni, Cyrille ULg; Gerrienne, Philippe ULg

in Special Publication - Geological Society of London (2010), 339

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See detailEarly seed plants from Western Gondwana: Paleobiogeographical and ecological implications based on Tournaisian (Lower Carboniferous) records from Argentina
Prestianni, C.; Rustan, J.J.; Balseiro, D. et al

in Palaeogeography, Palaeoclimatology, Palaeoecology (2015), 417

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See detailEarly sex reversal during the embryonic development in the Nile tilapia
Rougeot, Carole ULg; Kanfitine, Samane, Yadja; Prignon, Christian ULg et al

in Cybium (2008), 32(2), 104-105

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