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See detailEmbryo survival after transfer of in vitro and in vivo produced goat embryos
Cognié, Yves; Poulin, N.; Guignot, F. et al

in Proceedings: 17th Annual Meeting AETE (2001)

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See detailEmbryo transfer as a method to eliminate pathogenic agents in a rabbit colony
Ectors, Fabien ULg; Delfosse, Henri; De Weder, L. et al

in Vlaams Diergeneeskundig Tijdschrift (2002), 71

To regain the SPF status of a contaminated but genetically valuable rabbit breeding unit, embryos from the contaminated does were transferred into SPF recipient females. Embryos were collected on day 3 of ... [more ▼]

To regain the SPF status of a contaminated but genetically valuable rabbit breeding unit, embryos from the contaminated does were transferred into SPF recipient females. Embryos were collected on day 3 of gestation by flushing uterine horns. All usable embryos were frozen, part of them were not transferred and kept in liquid nitrogen forming a stock of highly valuable genotypes. Thirty-two stimulated does produced 893 embryos, among which 821 (92%) had an intact zona pellucida and were cryopreserved. From this stock, 478 embryos were thawed, 466 were recovered (97.5%) and 417 were of good quality (87.2%). In 30 does, 10 to 18 embryos were surgically transferred per recipient doe and 24.9% (104/417) of them developed to term after transfer. This corresponds to an average number of 3.47 (104/30) live newborns per recipient. Health screenings performed on sanitized rabbits confirmed the disappearance of pathogenic agents. [less ▲]

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See detailEmbryo transfer as a tool for experimental reproduction of ovine herds.
Rizzo, H.; François, D.; Fassier, T. et al

in Ciência Animal Brasileira (2009), Suppl 1

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See detailEmbryo-specific reduction of ADP-Glc pyrophosphorylase leads to an inhibition of starch synthesis and a delay in oil accumulation in developing seeds of oilseed rape.
Vigeolas, Hélène ULg; Mohlmann, Torsten; Martini, Norbert et al

in Plant Physiology (2004), 136(1), 2676-86

In oil-storing Brassica napus (rape) seeds, starch deposition occurs only transiently in the early stages of development, and starch is absent from mature seeds. This work investigates the influence of a ... [more ▼]

In oil-storing Brassica napus (rape) seeds, starch deposition occurs only transiently in the early stages of development, and starch is absent from mature seeds. This work investigates the influence of a reduction of ADP-Glc pyrophosphorylase (AGPase) on storage metabolism in these seeds. To manipulate the activity of AGPase in a seed-specific manner, a cDNA encoding the small subunit of AGPase was expressed in the sense or antisense orientation under the control of an embryo-specific thioesterase promoter. Lines were selected showing an embryo-specific decrease in AGPase due to antisense and cosuppression at different stages of development. At early developmental stages (25 days after flowering), a 50% decrease in AGPase activity was accompanied by similar decreases in starch content and the rate of starch synthesis measured by injecting (14)C-Suc into seeds in planta. In parallel to inhibition of starch synthesis, the level of ADP-Glc decreased, whereas Glc 1-phosphate levels increased, providing biochemical evidence that inhibition of starch synthesis was due to repression of AGPase. At 25 days after flowering, repression of starch synthesis also led to a decrease in the rate of (14)C-Suc degradation and its further metabolism via other metabolic pathways. This was not accompanied by an increase in the levels of soluble sugars, indicating that Suc import was inhibited in parallel. Flux through glycolysis, the activities of hexokinase, and inorganic pyrophosphate-dependent phosphofructokinase, and the adenylate energy state (ATP to ADP ratio) of the transgenic seeds decreased, indicating inhibition of glycolysis and respiration compared to wild type. This was accompanied by a marked decrease in the rate of storage lipid (triacylglycerol) synthesis and in the fatty acid content of seeds. In mature seeds, glycolytic enzyme activities, metabolite levels, and ATP levels remained unchanged, and the fatty acid content was only marginally lower compared to wild type, indicating that the influence of AGPase on carbon metabolism and oil accumulation was largely compensated for in the later stages of seed development. Results indicate that AGPase exerts high control over starch synthesis at early stages of seed development where it is involved in establishing the sink activity of the embryo and the onset of oil accumulation. [less ▲]

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See detailEmbryogenese precoce comparative lors des croisements entre Phaseolus coccineus L. et Phaseolus vulgaris L.
Ndoutoumou, P. N.; Toussaint, André ULg; Baudoin, Jean-Pierre ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2007), 11(2), 97-107

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See detailEmbryologie générale et moléculaire
Geenen, Vincent ULg

Learning material (2011)

Notes du cours d'Embryologie - M1BOE

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See detailEmbryonic development of kisspeptin neurones in rat.
Desroziers, Elodie ULg; Droguerre, M.; Bentsen, A. H. et al

in Journal of Neuroendocrinology (2012), 24(10), 1284-95

Kisspeptins, encoded by the Kiss1 gene, play a key role in the regulation of reproductive function, although very little is known about the ontogenesis of this system. The present study aimed to determine ... [more ▼]

Kisspeptins, encoded by the Kiss1 gene, play a key role in the regulation of reproductive function, although very little is known about the ontogenesis of this system. The present study aimed to determine the period of arcuate nucleus (ARC) kisspeptin cell birth and the embryonic stage and neuroanatomical sites of onset of kisspeptin immunoreactivity. Bromodeoxyuridine (BrdU) was administered to female rats at various gestational stages and double immunohistochemistry against kisspeptin and BrdU was performed on brain sections from their offspring. The period of neurogenesis of ARC kisspeptin neurones begun between embryonic day (E) 12.5 and E13.5, reached its peak at E15.5 and was not completely over at E17.5. Kiss1 mRNA was detected in mediobasal hypothalamic punches of embryos aged E14.5, E16.5, E18.5 and E22.5 by real-time reverse transcriptase-polymerase chain reaction. Accordingly, kisspeptin-immunoreactive (-IR) cells were consistently detected in the embryonic ARC from E14.5 and their number increased until E18.5 to reach approximately half the level observed in adults. Between E18.5 and E22.5, the number of kisspeptin-IR cells and hypothalamic Kiss1 expression significantly decreased, regardless of sex, and this decrease persisted until birth. Taken together, these results demonstrate that rat ARC kisspeptin neurones are born locally during an extended embryonic period and are able to synthesise kisspeptins rapidly after their birth, consistent with the hypothesis of a role during embryonic activation of the hypothalamic-hypophyseal-gonadal axis. A sex-independent decrease of kisspeptin-IR cell numbers was observed during the perinatal period, suggestive of important regulations of kisspeptin neurones around birth. [less ▲]

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See detailEmbryonic Protein Undernutrition by Albumen Removal Programs the Hepatic Amino Acid and Glucose Metabolism during the Perinatal Period in an Avian Model.
Willems, Els; Hu, Tjing-Tjing; Soler Vasco, Laura et al

in PloS one (2014), 9(4), 94902

Different animal models have been used to study the effects of prenatal protein undernutrition and the mechanisms by which these occur. In mammals, the maternal diet is manipulated, exerting both direct ... [more ▼]

Different animal models have been used to study the effects of prenatal protein undernutrition and the mechanisms by which these occur. In mammals, the maternal diet is manipulated, exerting both direct nutritional and indirect hormonal effects. Chicken embryos develop independent from the hen in the egg. Therefore, in the chicken, the direct effects of protein deficiency by albumen removal early during incubation can be examined. Prenatal protein undernutrition was established in layer-type eggs by the partial replacement of albumen by saline at embryonic day 1 (albumen-deprived group), compared to a mock-treated sham and a non-treated control group. At hatch, survival of the albumen-deprived group was lower compared to the control and sham group due to increased early mortality by the manipulation. No treatment differences in yolk-free body weight or yolk weight could be detected. The water content of the yolk was reduced, whereas the water content of the carcass was increased in the albumen-deprived group, compared to the control group, indicating less uptake of nutrients from the yolk. At embryonic day 16, 20 and at hatch, plasma triiodothyronine (T3), corticosterone, lactate or glucose concentrations and hepatic glycogen content were not affected by treatment. At embryonic day 20, the plasma thyroxine (T4) concentrations of the albumen-deprived embryos was reduced compared to the control group, indicating a decreased metabolic rate. Screening for differential protein expression in the liver at hatch using two-dimensional difference gel electrophoresis revealed not only changed abundance of proteins important for amino acid metabolism, but also of enzymes related to energy and glucose metabolism. Interestingly, GLUT1, a glucose transporter, and PCK2 and FBP1, two out of three regulatory enzymes of the gluconeogenesis were dysregulated. No parallel differences in gene expressions causing the differences in protein abundance could be detected pointing to post-transcriptional or post-translational regulation of the observed differences. [less ▲]

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See detailEmbryonic signals and pregnancy diagnosis in ruminants
Beckers, Jean-François ULg; Touati, Kamal ULg; Alvarez-Oxiley, A. V. et al

in Reproduction in Domestic Animals (2004), 39(4), 252

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See detailEmbryophytes on land: the early steps.
Gerrienne, Philippe ULg

Conference (2007)

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See detailEMCCD photometry reveals two new variable stars in the crowded central region of the globular cluster NGC 6981
Skottfelt, J.; Bramich, D. M.; Figuera Jaimes, R. et al

in Astronomy and Astrophysics (2013), 553

Two previously unknown variable stars in the crowded central region of the globular cluster NGC 6981 are presented. The observations were made using the electron multiplying CCD (EMCCD) camera at the ... [more ▼]

Two previously unknown variable stars in the crowded central region of the globular cluster NGC 6981 are presented. The observations were made using the electron multiplying CCD (EMCCD) camera at the Danish 1.54 m Telescope at La Silla, Chile. The two variableswere not previously detected by conventional CCD imaging because of their proximity to a bright star. This discovery demonstrates that EMCCDs are a powerful tool for performing high-precision time-series photometry in crowded fields and near bright stars, especially when combined with difference image analysis. Based on data collected by MiNDSTEp with the Danish 1.54 m telescope. [less ▲]

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See detailEmergence : trois interprétations théoriques
Santander, Sébastian ULg

Scientific conference (2012, April 25)

La présente communication revient sur les interprétations théoriques de la notion d'émergence en sciences sociales. Elle identifie trois interprétations: les économico-financières, les politologiques et ... [more ▼]

La présente communication revient sur les interprétations théoriques de la notion d'émergence en sciences sociales. Elle identifie trois interprétations: les économico-financières, les politologiques et les sociologiques. [less ▲]

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See detailL’émergence d’un marché d’immobilier d’entreprise. L’exemple de la Wallonie
Merenne-Schoumaker, Bernadette ULg

in Geocarrefour (2003), 78

In less than ten years, a business property market has emerged in Wallonia. This market first concerned offices and retail units (a sector not studied in this paper) and, more recently, semi-industrial ... [more ▼]

In less than ten years, a business property market has emerged in Wallonia. This market first concerned offices and retail units (a sector not studied in this paper) and, more recently, semi-industrial establishments. Its development is largely related to the creation of new demands in physical units, environment and accessibility, as well as in location choices, at both sub-regional and local scales. It has been favoured by the arrival of new property stakeholders from Belgium and abroad (developers, investors and property agents), as well as by public authorities (the Walloon Region and, more importantly, supra-communal, economic development agencies). The emergence of this market is related to the accentuation of territorial contrasts as property development projects usually reinforce strong area [less ▲]

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See detailEmergence de la coopération Sud-Sud. Vers une rupture avec la Nord?
Baenda Fimbo, Zacharie ULg

in Perspectives internationales (2013)

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