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See detailDetermination of mass-loss rates from the first order moment W1 of unsaturated Cygni line profiles
Surdej, Jean ULg

in Astrophysics & Space Science (1982), 88

The relationship between the first order moment and the mass loss rate is investigated within the framework of the Sobolev approximation (1947, 1957, 1958) under various physical and geometrical ... [more ▼]

The relationship between the first order moment and the mass loss rate is investigated within the framework of the Sobolev approximation (1947, 1957, 1958) under various physical and geometrical conditions. By assuming that the size of the expanding envelope from which the observed P Cygni profiles arise is large with respect to the central stellar core, a general expression for the n-th order moment is obtained for the case of a point-like source in terms of the usual parameters inherent to Sobolev-type theories. It is then shown how the first result is altered when considering separately the effects due to collisions and rotation, the presence of the underlying photospheric absorption line, the finite size of the central core, and the limb-darkening of the stellar core. It is concluded that the total uncertainty of the mass loss rate determination made with the approach proposed here should be less than 60 percent. [less ▲]

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See detailDetermination of mass-loss rates of planetary nebulae nuclei using the first order moment of P Cygni line profiles.
Hutsemekers, Damien ULg; Surdej, Jean ULg

in Comptes rendus sur les Journées de Strasbourg, 8ème réunion - Les Nébuleuses Planétaires (1986)

The authors conclude that the mass-loss rates of planetary nebulae nuclei derived by Cerruti-Sola, Perinotto (1985) have been systematically underestimated by one or nearly two orders of magnitude.

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See detailThe Determination of Megakaryocyte Ploidy
Paulus, Jean-Michel ULg; Senterre, J. M.; von Frenckell, R.

in International Journal of Cell Cloning (1990), 8(4), 227-35

Methods which have been used to determine megakaryocyte ploidy in animals and humans are reviewed. Although the number of megakaryocyte nuclear units counted in bone marrow squashes is roughly ... [more ▼]

Methods which have been used to determine megakaryocyte ploidy in animals and humans are reviewed. Although the number of megakaryocyte nuclear units counted in bone marrow squashes is roughly proportional to ploidy, accurate determinations of DNA content require the use of microphotometry or flow cytometry. New techniques should make it possible to distinguish polyploidizing megakaryoblasts from promegakaryocytes and mature megakaryocytes which have arrested polyploidization. Only the latter should be included in histograms of the number of endoduplications, since only those have expressed their full polyploidization potential. Statistical techniques are available for analysis and comparison of both raw ploidy distributions or histograms of endoduplication numbers. [less ▲]

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See detailDetermination of Meprobamate in Pharmaceutical Dosage Forms Also Containing Carbromal by Liquid Chromatography and Indirect Photometric Detection
Bechet, I.; Ceccato, Attilio ULg; Hubert, Philippe ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (1992), 10(10-12, Oct-Dec), 995-9

In a pharmaceutical form also containing carbromal, meprobamate could not be quantified selectively by classical methods described in pharmacopoeias due to a significant interference from carbromal ... [more ▼]

In a pharmaceutical form also containing carbromal, meprobamate could not be quantified selectively by classical methods described in pharmacopoeias due to a significant interference from carbromal. Consequently, reversed-phase HPLC methods have been developed to separate the two active ingredients using indirect photometric detection to visualize and determine meprobamate which has very poor chromophoric properties. Different parameters influencing the sensitivity of the indirect response, such as the nature of the highly absorbing compound added to the mobile phase (the marker) as well as the methanol content and the pH of this phase, have been studied. Two chromatographic systems containing benzoic acid or cinnamic acid as the marker, have been optimized and validated. Good linearity and reproducibility have been obtained with both systems but the cinnamic acid method has the advantage that meprobamate and carbromal can be determined simultaneously at 273 nm. [less ▲]

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See detailDetermination of methionine requirement of growing double-muscled Belgian Blue bull.
Froidmont, Eric; Beckers, Yves ULg; Thewis, André ULg

in Book of abstract of the VIIIth International Symposium on Protein Metabolism and Nutrition. (1999)

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See detailDetermination of minimal serum estradiol for prevention of postmenopausal bone loss
Sarlet, N; Reginster, Jean-Yves ULg; GASPARD, Ulysse ULg et al

in Calcified Tissue International (1989), 44

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See detailDetermination of minimum effective doses of luteinizing hormone and human chorionic gonadotropin for intrafollicular treatment to induce ovulation in dairy heifers.
Mala, J; Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg et al

in Acta Veterinaria BRNO (2013), 82

The aim of this study was to determine the minimum effective intrafollicular doses of luteinizing hormone and human chorionic gonadotropin in order to induce ovulation in cycling dairy heifers that have ... [more ▼]

The aim of this study was to determine the minimum effective intrafollicular doses of luteinizing hormone and human chorionic gonadotropin in order to induce ovulation in cycling dairy heifers that have not yet been adequately established. Application of 10, 5, 1, 0.5, 0.1, 0.01 and 0.001 µg luteinizing hormone as well as 10, 1, 0.1, 0.01 and 0.001 international units (IU) of human chorionic gonadotropin in dominant follicles was performed on day 7 of the oestrous cycle. Control animals were given luteinizing hormone (12.5 mg and 25 mg) or human chorionic gonadotropin (2000 IU) intravenously. Accessory corpus luteum on day 14 of the oestrous cycle was considered as an evidence of ovulation. Ovulation was observed in 2 out of 3 heifers in each treatment group (n = 3) after administration of 10–0.1 µg luteinizing hormone (except for 0.5 µg – ovulation in 3 of 3 heifers), in all heifers after administration of 10–0.01 IU human chorionic gonadotropin as well as in all control heifers. Administration of 0.01 µg and 0.001 µg luteinizing hormone as well as of 0.001 IU human chorionic gonadotropin did not result in ovulation. Higher progesterone concentration on day 14 vs. day 7 of the oestrous cycle was found after all treatments. Nevertheless, the differences were signicant (P < 0.05) only after intrafollicular treatments with 5, 1 and 0.001 µg luteinizing hormone as well as 10, 1 and 0.01 IU human chorionic gonadotropin. In conclusion, minimum efcient doses for intrafollicular treatment of the dominant follicles in cycling heifers capable of inducing ovulation were 0.1 µg of luteinizing hormone and 0.01 IU of human chorionic gonadotropin. This is the rst study describing the intrafollicular luteinizing hormone administration in cycling dairy heifers. [less ▲]

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See detailDetermination of Moisture Diffusivity During Drying of Mortar Cement: Experimental and Modeling Study
Bennamoun, Lyes ULg; Kahlerras, Loubna ULg; Michel, Frédéric ULg et al

in International Journal of Energy Engineering (2013), 3(1), 1-6

The aim of this study is the experimental characterization of the behaviour of a mortar during convective drying. We focalise on mortar that has a rate water-cement of 0.5. The drying tests are developed ... [more ▼]

The aim of this study is the experimental characterization of the behaviour of a mortar during convective drying. We focalise on mortar that has a rate water-cement of 0.5. The drying tests are developed in a micro-convective dryer that can use samples weighing from 0 to 8g. The advantage of these experiments is to reproduce the natural conditions that can be found during the treatment of the mortar-atmosphere problems. The response of the drying curve or the drying kinetic depends on the applied drying conditions. So, the temperature of the air varies from 60°C to 130°C, the velocity of the air is changed from 2 m.s-1 to 5 m. s-1and the relative humidity is kept less than 1.7%. The comparison between the experimental results and the proposed analytical solutions of the equation of diffusion represented by Fick’s law, applied for a finite shape, allows determination of the values of the diffusion coefficient. It has a value of 1.71×10-10 m2.s-1 at 60°C, 13.69×10-10 m2.s-1 at 90°C and 16.27×10-10 m2.s-1 at 130°C. Calculation of the activation energy and the D0 constant are also possible. [less ▲]

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See detailDetermination of muscle mitochondrial respiratory capacity in Standardbred racehorses as an aid to predicting exertional rhabdomyolysis
Houben, Rosa ULg; Leleu, Claire; Fraipont, Audrey ULg et al

in Mitochondrion (2015), 24

This prospective cohort study evaluated the potential of high-resolution respirometry applied to permeabilized muscle fibers for fitness evaluation in French Standardbred racehorses. Fitness evaluation by ... [more ▼]

This prospective cohort study evaluated the potential of high-resolution respirometry applied to permeabilized muscle fibers for fitness evaluation in French Standardbred racehorses. Fitness evaluation by means of respirometric parameters did not correlate with racing performance registered over the following racing season. However, altered mitochondrial energy metabolism was associated with higher risk of developing exertional rhabdomyolysis, a common cause of exercise intolerance in racehorses. These data represent a first step towards establishing reference values for muscle OXPHOS capacity in this breed. [less ▲]

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See detailDetermination of myoglobin in serum by kinetic turbidimetry, using the turbitimesystem
Chapelle, Jean-Paul ULg; El Allaf, M.

in Clinical Chemistry (1990), 36(6), 1193

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See detailDetermination of N-acetylcysteine and its metabolites in plasma by LC-MS-MS
Toussaint, B.; Streel, B.; Ceccato, Attilio ULg et al

Poster (2000)

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See detailDetermination of N-acetylcysteine and its potential degradation products by LC/APCI+/MS/MS
Toussaint, B.; Ceccato, Attilio ULg; Maes, P. et al

Conference (1997)

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See detailDetermination of Non-Steroidal Anti-Inflammatory Drugs by Capillary Zone Electrophoresis
Bechet, I.; Paques, Ph.; Fillet, Marianne ULg et al

Poster (1994)

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See detailDetermination of optimal load shedding against voltage instability
Moors, Cédric; Van Cutsem, Thierry ULg

in Proc. 13th Power System Computation Conference (PSCC) (1999, June)

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See detailDetermination of optimum conditions for preservation and reactivation of freeze-dried Acetobacter senegalensis used as vinegar starter
Shafiei, Rasoul ULg; Thonart, Philippe ULg

Poster (2011, November)

Availability of efficient starters is one of the most important elements during fermentation technology. Acetic acid bacteria (AAB) face extreme conditions (low pH, high acetic acid concentration (>7 ... [more ▼]

Availability of efficient starters is one of the most important elements during fermentation technology. Acetic acid bacteria (AAB) face extreme conditions (low pH, high acetic acid concentration (>7%), ethanol and high temperature) during vinegar fermentation. In spite of introduction of broth media contained viable AAB as starter by some companies; traditional methods for initiation of vinegar fermentation are still used even in modern factories, therefore there is a long Lag phase for initiation of fermentation. In recent studies in CWBI, a kind of lyophilized starter has been produced by using an isolated thermotolerant bacterium: Acetobacter senegalensis. The aim of this study is to determine the best media for revitalization and the optimum temperature for preservation of the freeze-dried cells. To produce biomass, glucose was used as carbon source in batch culture under regulated conditions. The cells harvested when they reached to stationary phase. Harvested cells suspended in spent growth medium (final supernatant of fermentation). Maltodextrin (10%), manitol (20%) and spent growth medium were used as protestants. After freeze-drying they were subjected to storage test at -20° C, 4° C and 35° C for six months. The viability of cells determined by using spread plate technique using three different media: YG (yeast extract, glucose) and YGE (yeast extract, glucose, ethanol) and YGEA (yeast extract, glucose, ethanol, acetic acid). According to residual viable cells, manitol and maltodextrin showed higher protective functions rather than spent growth medium (92.3%, 88.2% and 82.1% survival, respectively) during freeze-drying process. Viability of cells during rehydration is completely dependent to the composition of the broth media used for this purpose. Adding ethanol (>0.5%) or acetic acid (>1%) to rehydration medium caused 40-45% reduction in viable cell numbers in comparison to YG broth. The composition of culture media can also influence the growth of bacteria after rehydration. In YG, much higher cell growth (about 1 log unit) was observed in comparison to YGE and YGEA. Addition of glucose (20 g/l) to YGE can neutralize the adverse effect of ethanol considerably but it cannot improve the growth in YGEA. This can be partially explained according to the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase. It was revealed that freeze-dried cells had very low activities for these two enzymes. Analysis of total soluble protein contents of lyophilized cells during storage at different conditions revealed that the soluble protein content of cells reduced by increasing the storage temperature. At 35° C after 15 days, there were 4.87 log units reduction in the viability of cells, and also about 14% less soluble protein was detectable under this condition whereas keeping of cells at -20 or + 4° C had no influences on protein and viability of cells. Storage of cells at +4° C in darkness for six month showed that about 79.2% and 68.3% viability of cells remained for cells protected by manitol and maltodextrin, respectively. To assess the real activity of lyophilized cells as starters, they were introduced to broth media (YGE contained 20g/l of glucose). It was seen that 6 months old lyophilized cells (with maltodextrin or manitol as protectants) were able to grow and consume 5 v/v of ethanol in 48 hr in the presence of initial acetic acid concentration (0.5%) at 30° C. In conclusion, the mentioned methods for preservation and reactivation of freeze-dried Acetobacter senegalensis can provide a promising tool for decreasing the lag phase of vinegar fermentation. [less ▲]

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See detailDetermination of organochlorine pesticide residues in the blood of healthy individuals
Charlier, Corinne ULg; Plomteux, Guy ULg

in Clinical Chemistry & Laboratory Medicine (2002), 40(4), 361-364

Pesticide use is one of several factors that have permitted maintenance of our supply of food in spite of continuing increase of the population. However, the use of biologically active compounds poses ... [more ▼]

Pesticide use is one of several factors that have permitted maintenance of our supply of food in spite of continuing increase of the population. However, the use of biologically active compounds poses potential problems of toxicity. If the compound is used at any stage of food production, residues or derivatives may persist in food and the entire population may be exposed to the trace amounts of the material. The human body burden associated with long-term exposure may or may not be associated with illness. Persistent environmental contaminants such as pesticide residues have long been suspected to be implicated in cancer etiology. Organochlorine chemicals are persistent, lipophilic compounds commonly present in the environment. Some of them demonstrated carcinogenic activity in laboratory animals. Controversy still exists concerning their carcinogenic potential in humans. To answer this question, clinical toxicology laboratories should propose validated methodologies able to identify and quantify pesticide residues in biological samples. An example of chromatographic method dedicated to organochlorine residues is presented here and illustrated by results obtained in a healthy population (104 men, 147 women). Only 17.9% of the samples were free from detectable amounts of pesticides and p,p’-DDE 1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene was the most frequently detected residue (66.5%). Hexachlorobenzene was found at detectable level in 13.5% of the samples. [less ▲]

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See detailDetermination of organohalogenated contaminants in liver of harbour porpoises (Phocoena phocoena) stranded on the Belgian North Sea coast
Covaci, Adrian; Van de Vijver, Kristin Inneke; De Coen, Wim et al

in Marine Pollution Bulletin (2002), 44(10),

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See detailDetermination of PAG in the milk and blood in cow’s in comparison to other examination methods for pregnancy diagnosis.
Gajewski, Z.; Melo de Sousa, Noelita ULg; Konopka, A. A. et al

in Magyar Allatorvosok Lapja = Hungarian Veterinary Journal (2008), (Suppl II), 190

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