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See detailEffect of potassium channel openers on the firing rate of hippocampal pyramidal cells and A10 dopaminergic neurons in vitro.
Scuvée-Moreau, Jacqueline ULg; Seutin, Vincent ULg; Vrijens, Bernard ULg et al

in Archives of Physiology & Biochemistry (1997), 105(5), 421-8

The effect of four KATP channel openers (KCOs) on the firing rate of CA1 pyramidal cells and A10 dopaminergic neurons was investigated using extracellular recording techniques in rat brain slices ... [more ▼]

The effect of four KATP channel openers (KCOs) on the firing rate of CA1 pyramidal cells and A10 dopaminergic neurons was investigated using extracellular recording techniques in rat brain slices. Pinacidil, lemakalim, diazoxide and a new compound, BPDZ44, had an inhibitory effect on the electrical activity of CA1 pyramidal cells. They all had a similar potency. Only BPDZ44 and diazoxide inhibited the firing rate of A10 dopamine neurons. The sulfonylurea glipizide (1 microM) antagonized the effect of BPDZ44 and diazoxide on A10 neurons but failed to antagonize the effect of KCOs on CA1 pyramidal cells. These results show that differences exist among KCOs in their ability to decrease the electrical activity of various populations of central neurons. [less ▲]

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See detailEffect of potential drugs on model membranes - An AFM study
Eeman, Marc; Deleu, Magali ULg; Paquot, Michel ULg et al

Poster (2005)

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See detailEffect of ppMCH derived peptides on PBMC proliferation and cytokine expression
Coumans, Bernard ULg; Grisar, Thierry ULg; Nahon, J. L. et al

in Regulatory Peptides (2007), 143(1-3), 104-108

The mRNA encoding prepro-Melanin concentrating hormone (ppMCH) is mainly expressed in the central nervous system but has also been detected at lower amount in many peripheral tissues including spleen and ... [more ▼]

The mRNA encoding prepro-Melanin concentrating hormone (ppMCH) is mainly expressed in the central nervous system but has also been detected at lower amount in many peripheral tissues including spleen and thymus. At the peptide level however, several forms of the precursor can be detected in these tissues and are sometimes expressed at similar levels compared to brain. In the present work, we have studied the in vitro action of a wide range of concentration (1 nM to 1 microM) of the different peptides encoded by ppMCH i.e. neuropeptide glycine-glutamic acid (NGE), neuropeptide glutamic acid-isoleucine (NEI), Melanin concentrating hormone (MCH) and the dipeptide NEI-MCH on peripheral blood mononuclear cells (PBMC) proliferation and cytokine production following anti-CD3 stimulation. Among them only MCH decreased PBMC proliferation with a maximal effect of 35% at 100 nM. Moreover as demonstrated by using ELISA, MCH significantly decreases IL-2 production by 25% but not IL-4, INF-gamma or TNF-alpha expression. Interestingly, exogenous IL-2 decreases significantly MCH-mediated inhibition, suggesting that it is an important downstream mediator of MCH action. Finally, we showed that after 7 to 9 days of incubation, MCH also inhibits proliferation of non-stimulated PBMC. Altogether, these data demonstrate that fully mature MCH modulates proliferation of anti-CD3 stimulated PBMC partially through regulation of IL-2 production. [less ▲]

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See detailEffect of pps disruption and constitutive expression of srfA on surfactin productivity, spreading and antagonistic properties of Bacillus subtilis 168 derivatives
Coutte, F.; Leclere, V.; Bechet, M. et al

in Journal of Applied Microbiology (2010), 109

Aims: To analyse the effects of plipastatin operon disruption and constitutive expression of surfactin operon in Bacillus subtilis 168 on surfactin productivity, in vitro invasive growth and antagonism ... [more ▼]

Aims: To analyse the effects of plipastatin operon disruption and constitutive expression of surfactin operon in Bacillus subtilis 168 on surfactin productivity, in vitro invasive growth and antagonism against fungi. Methods and Results: The srfA native promoter was replaced by the constitutive promoter PrepU in B. subtilis 168 after integration of a functional sfp gene. Moreover, the plipastatin synthesis was further disrupted in the B. subtilis 168 derivatives. In liquid media, an earlier and higher expression of PrepU, than that found with PsrfA, led to a specific surfactin production fivefold higher after 6 h of culture. On solid media, not only the invasive growth and the haemolytic activity but also the antifungal activity of the constitutive strains were improved when compared to the parental strain BBG111. As expected, the disruption of the plipastatin operon strongly reduced in vitro antifungal properties but, interestingly, enhanced specific surfactin production (1Æ47 g g)1 of biomass), spreading behaviour and haemolytic activity of the strains. Conclusions: This work demonstrates for the first time the interdependency of surfactin and plipastatin regarding their biosynthesis as well as their influence on the biological activities of the producing strain. Significance and Impact of the Study: The constitutive overproduction of surfactin enhances the invasive growth and the in vitro antagonistic activity of the mutant strain. Both properties are known to play an important role in the biocontrol of plant diseases. Plipastatin operon disruption increases the surfactin productivity of mutant strains. These mutants are interesting for use in continuous bioprocesses for surfactin production or in bioremediation. [less ▲]

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See detailEffect of pre-emptive Vagus Nerve Stimulation on cortical spreading depression in rat
Multon, Sylvie ULg; Prodanov, D.; Chauvel, Virginie ULg et al

Poster (2008, September)

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See detailEffect of precolonization of bean seeds with Trichoderma, on symptoms induced by Pythium.
Cotes, A. M.; Lepoivre, Philippe ULg; Semal, J.

in Mededelingen van de Faculteit Landbouwwetenschappen (Rijksuniversiteit te Gent) (1992), 57(2b),

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See detailEffect of prenatal androgen receptor antagonist or aromatase inhibitor on the differentiation of neuronal Fos responses to estrous female pheromones in the rat accessory olfactory system
Dominguez, Emilio; Portillo, Wendy; Baum, Michael J et al

in Physiology & Behavior (2002), 75

Many socially relevant odors are detected in rodent species by the vomeronasal organ and subsequently processed by the accessory olfactory system (AOS). We previously found that gonadectomized male and ... [more ▼]

Many socially relevant odors are detected in rodent species by the vomeronasal organ and subsequently processed by the accessory olfactory system (AOS). We previously found that gonadectomized male and female rats treated in adulthood with testosterone propionate (TP) showed equivalent Fos responses in the AOS to odors derived from estrous females. Likewise, in contrast with numerous other mammalian species, gonadectomized female rats show surprisingly high levels of male-typical mounting behavior in response to adult TP. We tested the hypothesis that prenatal testosterone (T) exposure, acting via androgen receptors (ARs) or via estrogen receptors, masculinizes the AOS in rats of both sexes. Pregnant dams were treated with either the AR blocker, Flutamide, the aromatase inhibitor, 1,4,6-androstatriene-3,17-dione (ATD), or nothing (control) to assess the role of prenatal androgen and estradiol receptor activation, respectively, in this masculinization. Beginning at birth, male and female offspring were injected subcutaneously (sc) every other day with either ATD (pre- and neonatal ATD group) or oil vehicle (Flutamide and control groups) until postnatal Day 12. Subjects were gonadectomized as adults, hormonally treated and tested for different behaviors before having their AOS Fos responses to estrous female odors assessed. Prenatal treatment with Flutamide (but not ATD) significantly decreased anogenital distance and severely impaired intromissive and ejaculatory behaviors in males tested after TP replacement without disrupting mounting capacity in either sex. Pre- and neonatal treatment with ATD (but not Flutamide) enhanced lordosis responsiveness in males tested after sc injections of estradiol and progesterone, whereas these perinatal treatments had no effect on any aspect of masculine coital performance in either sex. After TP treatment, male and female control subjects preferred to approach a tethered stimulus female as opposed to a male, and prenatal Flutamide or perinatal ATD treatments did not modify this pattern of partner preference. Neuronal Fos responses to estrous odors were (as in previous studies) identical in the AOS of gonadectomized TP-treated control males and females. Prenatal Flutamide or perinatal ATD treatments failed to disrupt consistently this profile of Fos responses to estrous odors in the AOS of rats of either sex. These behavioral and neuroanatomical findings raise the possibility that the similar level of male-typical responsiveness to social odors that occurs in male and female rats after adult TP treatment results from nonsteroid-hormone-dependent, species-specific factors that act perinatally in the brains of rats of both sexes. [less ▲]

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See detailEffect of prenatal undernutrition of the chicken embryo on growth and metabolism up to day 7
Willems, Els; Willemsen, Hilke; Li, Yue et al

Poster (2011)

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See detailEffect of prenatal undernutrition of the chicken embryo on postnatal performance up to day 21
Willems, Els; Willemsen, Hilke; Wang, Yufeng et al

in The 5th combined workshop: Fundamental Physiology of the European working group of physiology and perinatal development in poultry (2011)

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See detailEffect of pressure and temperature upon tin alkoxide-promoted ring-opening polymerisation of epsilon-caprolactone in supercritical carbon dioxide
Stassin, Fabrice; Jérôme, Robert ULg

in Chemical Communications (2003), (2), 232-233

Ring-opening polymerisation of epsilon-caprolactone in supercritical carbon dioxide is slowed down by a carbonation reaction, resulting in a positive volume of activation and a higher energy of activation ... [more ▼]

Ring-opening polymerisation of epsilon-caprolactone in supercritical carbon dioxide is slowed down by a carbonation reaction, resulting in a positive volume of activation and a higher energy of activation as compared to polymerisation in a regular hydrocarbon solvent. [less ▲]

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See detailThe effect of pressure on DSr (plag/melt) and DCr (opx/melt): implications for anorthosite petrogenesis
Vander Auwera, Jacqueline ULg; Longhi, John; Duchesne, Jean-Clair ULg

in Earth & Planetary Science Letters (2000), 178

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See detailEffect of pressure, temperature, fluorine doping, and rare earth elements on the phonon density of states of LFeAsO studied by nuclear inelastic scattering
Sergueev, I.; Hermann, Raphael ULg; Bessas, D. et al

in PHYSICAL REVIEW B (2013), 87(6), 064302-9

We have performed systematic studies of the lattice dynamics in LFeAsO (L = La, Ce, Pr, Nd, Sm) in the parent and in the similar to 10% F-doped compounds as a function of pressure and temperature. We have ... [more ▼]

We have performed systematic studies of the lattice dynamics in LFeAsO (L = La, Ce, Pr, Nd, Sm) in the parent and in the similar to 10% F-doped compounds as a function of pressure and temperature. We have found that the modifications in the partial Fe density of phonon states are mainly governed by the Fe-As bond length. The change of this bond length explains the change of the Fe density of phonon states above 25 meV. We further observe anomalies in the behavior of the phonon mode near 16 meV. In the parent phase, this mode softens anomalously upon cooling through the structural phase transition. Upon F doping, this mode hardens indicating a strong electron-phonon coupling. This suggests that the corresponding phonons play an important role in the competition between superconductivity and magnetism in these materials. DOI: 10.1103/PhysRevB.87.064302 [less ▲]

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See detailEffect of preventive antioxidant supplementation on lung function, airway inflammation and pulmonary 8-epi-PGF2 of calves exposed to ozone
Kirschvink, Nathalie; Fievez, Laurence ULg; Jacqmot, O. et al

in Proceedings: Réunion de la Société Belge de Physiologie et de Pharmacologie Fondamentales et Cliniques (2001)

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See detailEffect of previous antidepressant therapy on the growth hormone response to apomorphine.
Pitchot, William ULg; Hansenne, Michel ULg; Gonzalez Moreno, A. et al

in Neuropsychobiology (1995), 32(1), 19-22

Several lines of evidence suggest a role for dopamine in the pathophysiology of depression. In 1988, we reported a blunted response of growth hormone (GH) to apomorphine, a dopaminergic agonist, in ... [more ▼]

Several lines of evidence suggest a role for dopamine in the pathophysiology of depression. In 1988, we reported a blunted response of growth hormone (GH) to apomorphine, a dopaminergic agonist, in endogenous depression. However, an antidepressant washout period is a major confounding factor in studies assessing the GH response to apomorphine. Indeed, whereas the influence of tricyclic antidepressants on the GH response to apomorphine is presently unknown, several reports have suggested that tricyclics may impair the GH response to clonidine for periods longer than 3 weeks following their discontinuation. In the present study, we hypothesized that a blunted GH response to apomorphine in depressed patients could be related to the recent administration of antidepressants. Therefore, the GH response to apomorphine (0.5 mg) was studied in 11 male DSM-III-R major depressive inpatients who had never received antidepressant therapy (group 1) compared to 11 normal controls and 11 major depressive inpatients drug free for at least 2 weeks (group 2). The three groups differed significantly in the GH peak response to apomorphine: mean (SD) 5.4 (4.0) ng/ml in group 1, 25.5 (10.7) in normal controls, and 5.5 (5.1) in group 2 (F = 15.5, df = 3, 30, p = 0.00001). While group 1 and normal controls (F = 21.8, p = 0.0002) as well as group 2 and controls (F = 5.6, p = 0.03) differed significantly, group 1 and group 2 did not (F = 0.18, p = 0.68). These results suggest that a washout period of 2 weeks could be sufficient in studies assessing the GH response to apomorphine. [less ▲]

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See detailEffect of processing conditions on phenolic compounds and antioxidant properties of date syrup
Abbès, F.; Kchaou, W.; Blecker, Christophe ULg et al

in Industrial Crops & Products (2013), 44

Commercial hydrolytic enzymes (pectinase and cellulase) were investigated for their influence on phenolics and antioxidant activities of date syrup. Content of total phenolics, flavonoids and carotenoids ... [more ▼]

Commercial hydrolytic enzymes (pectinase and cellulase) were investigated for their influence on phenolics and antioxidant activities of date syrup. Content of total phenolics, flavonoids and carotenoids contents were determined spectrophotometrically. Polyphenols composition was examined using HPLC. Seven phenolic compounds (catechin, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid and sinapic acid) were detected in all date syrup extracts. p-Coumaric acid was the major compound of all date syrup extracts, representing 49.223-63.397%. The antioxidant activity of date syrup was evaluated by various antioxidant assays, including total antioxidant, DPPH scavenging test, FRAP, hydrogen peroxide scavenging activity and metal chelating activity.Results showed that the date syrup prepared after extraction with pectinase and cellulase mixture gave the lowest phenolic and flavonoid contents. For same variety, date syrup prepared after extraction with pectinase and cellulase mixture gave the highest carotenoids amounts. The antioxidant activity, determined by several methods, was significantly affected by the extraction method as well as date varieties. In fact, the date syrup prepared after extraction with pectinase and cellulase mixture gave the lowest antioxidant activity. A good correlation between the antioxidant activity and total phenolic content and flavonoid was observed. The discovery of our research is very important to scientists as well as to the fruit-processing industry to produce juice and syrup with high antioxidant activity. © 2012 Elsevier B.V. [less ▲]

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See detailThe effect of processing on inflammatory markers in induced sputum.
Louis, Renaud ULg; Shute, J.; Goldring, K. et al

in European Respiratory Journal (1999), 13(3), 660-7

The effects of the mucolytic agent, dithioerythritol (DTE), and the temperature at which sputum processing is conducted on cellular and biochemical markers in induced sputum was assessed. Samples from ... [more ▼]

The effects of the mucolytic agent, dithioerythritol (DTE), and the temperature at which sputum processing is conducted on cellular and biochemical markers in induced sputum was assessed. Samples from healthy and atopic asthmatic subjects were treated with either DTE or phosphate-buffered saline (PBS) at 22 or 37 degrees C and compared for cell counts and concentrations of histamine, tryptase, eosinophil cationic protein (ECP), free interleukin (IL)-8, immunoglobulin (Ig)A, IL-8/IgA complexes and secretory component (SC). In addition, the influence of DTE on in vitro mediator release from blood eosinophils, basophils and bronchoalveolar lavage (BAL) mast cells was studied. Processing with DTE improved cytospin quality and increased the cell yield and measurable ECP, tryptase, IgA and SC, but reduced levels of histamine in PBS-treated samples and had no effect on IL-8. Cell counts or mediator levels were similar when sputum was processed at 22 or 37 degrees C, even though DTE induced blood basophils and BAL mast cells to release histamine at 37 degrees C. In spiking experiments, recovery of added ECP, tryptase, total IL-8 and histamine from sputum was similar in DTE- and PBS-processed sputum, but reduced for free IL-8 in PBS-treated samples. In conclusion, dithioerythritol improves cell and mediator recovery without causing cell activation when sputum processing is conducted at room temperature. The extent of recovery depends on the mediator studied. [less ▲]

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See detailEffect of prolactin on alpha and beta chloride cells in the gill epithelium of the saltwater adapted tilapia " Oreochromis niloticus"
Pisam, B.; Auperin, B.; Prunet, P. et al

in Anatomical Record (1993), 235

Tilapia (Oreochromis niloticus), 21 g average body weight, were divided into two groups. A group was maintained in fresh water, whereas another group was adapted for 2 weeks to 20% salt water. Among the ... [more ▼]

Tilapia (Oreochromis niloticus), 21 g average body weight, were divided into two groups. A group was maintained in fresh water, whereas another group was adapted for 2 weeks to 20% salt water. Among the latter, fishes were injected every 2 days for a week with tilapia prolactin (ti-PRL I). Gills were prepared for electron microscopy in order to determine the types and surface areas of chloride cells in each experimental condition. Two types of chloride cells, the alpha and beta cells were easily distinguished on the basis of their location and ultrastructural features in the gills of freshwater fishes, while only one type of cell, the saltwater alpha cells presumably derived from the transformation of the freshwater alpha cells, were encountered in saltwater adapted animals. After PRL injection of saltwater adapted fishes, small chloride cells, which displayed ultrastructural features similar to those of beta cells in freshwater tilapia, reappeared in interlamellar regions of the gills. In the same experimental conditions, the voluminous saltwater alpha cells showed a tendency to resume ultrastructural features more characteristic of the freshwater alpha cells from which they were derived. These observations tend to indicate that prolactin behaves as a "freshwater adapting hormone" and that beta cells are specifically involved in fish adaptation to freshwater living conditions. [less ▲]

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See detailEffect of prolonged exercise on neutrophil myeloperoxidase secretion
Bury, Thierry ULg; PIRNAY, Freddy ULg

in International Journal of Sports Medicine (1995)

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