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See detailDevelopment of anti-HPV lipoplexes for the treatment of cervical cancer
Lechanteur, Anna ULg; Furst, Tania ULg; Evrard, Brigitte ULg et al

Conference (2013, December 03)

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See detailDevelopment of aptamer-based radiopharmaceuticals for targeted cancer imaging and therapy
Gijs, Marlies ULg; Aerts, An; Impens, Nathalie et al

Poster (2013, March 04)

This SELEX experiment, for the selection of HER2 targeting aptamers, resulted in 26 selected RNA aptamers for further individual evaluation. Up till now, we were able to identify two aptamers that show ... [more ▼]

This SELEX experiment, for the selection of HER2 targeting aptamers, resulted in 26 selected RNA aptamers for further individual evaluation. Up till now, we were able to identify two aptamers that show binding to HER2 overexpressing SK-OV-3 cancer cells. In addition, we were able to generate silenced SK-OV-3 cells with minimal remaining HER2 levels, which will be used to obtain more information regarding the binding specificity of the selected aptamers. [less ▲]

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See detailDevelopment of aquaculture in North Vietnam: influence on the living standards and gender division of family labor
Caulier, Henri; Tran Thi Thap, Hieu; Tran Thi Nang, Thu et al

Poster (2016)

During previous years, along with the overall development of Vietnam, the fisheries sector has made great progress in terms of output and has remained as a very important sector of Vietnam’s economy. The ... [more ▼]

During previous years, along with the overall development of Vietnam, the fisheries sector has made great progress in terms of output and has remained as a very important sector of Vietnam’s economy. The average growth rate of this sector has been 7.9% per year –contributing greatly to the industrialization and modernization of the agriculture and rural sectors of the country. Since 2006, Vietnam has been globally ranked among the top 10 leading exporting countries in fisheries. To cope with these new circumstances, the aquaculture sector must grow while reducing emissions and pollution. Moreover, the value they generate should be distributed fairly, in order to improve the living conditions of producers, while the risks must be shared among all stakeholders. In this context, il will be also appropriate to conduct specific research including gender analysis of aquaculture value chain. The main objective of this paper is to present the main results of a field research conducted in the northern Vietnam and related to a specific fresh water aquaculture production system. This study was conducted to (1) describe and understand the division of family labor in aquaculture and (2) make a diagnose about economic situation of the households in aquaculture. [less ▲]

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See detailDevelopment of Astrocytes in the Sheep Cerebellum
Salouci, Moustafa ULg; Antoine, Nadine; Shikh Al Sook, Mohamad Khir ULg et al

Scientific conference (2013, October 11)

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See detailDevelopment of atherosclerosis for the vascular surgeon.
Defraigne, Jean-Olivier ULg

in Liapis, C. D. (Ed.) European Manual of Medicine (2007)

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See detailThe development of automatic numerosity processing in preschoolers : evidence for numerosity-perceptual interference
Rousselle, Laurence ULg; Noël, Marie-Pascale

in Developmental Psychology (2008), 44

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See detailDevelopment of B-lineage predominant lentiviral vectors for use in genetic therapies for B cell disorders
Sather, B. D.; Ryu, B. Y.; Stirling, B. V. et al

in Molecular Therapy : The Journal of the American Society of Gene Therapy (2011), 19(3), 515-25

Sustained, targeted, high-level transgene expression in primary B lymphocytes may be useful for gene therapy in B cell disorders. We developed several candidate B-lineage predominant self-inactivating ... [more ▼]

Sustained, targeted, high-level transgene expression in primary B lymphocytes may be useful for gene therapy in B cell disorders. We developed several candidate B-lineage predominant self-inactivating lentiviral vectors (LC) containing alternative enhancer/promoter elements including : the immunoglobulin β (Igβ) promoter combined with the immunoglobulin µ enhancer (EµB29); and the endogenous BTK promoter with or without Eµ (EµBtkp or Btkp). LV-driven enhanced green fluorescent protein (eGFP) reporter expression was evaluated in cell lines and primary cells derived from human or murine hematopoietic stem cells (HSC). In murine primary cells, eµB29 and EµBtkp LV-mediated high-level expression in immature and mature B cells compared with all other lineages. Expression increased with B cell maturation and was maintained in peripheral subsets. Expression in T and myeloid cells was much lower in percentage and intensity. Similarly, both EµB29 and EµBtkp LV exhibited high-level activity in human primary B cells. In contrast to EµB29, Btkp and EµBtkp LV also exhibited modest actiity in myeloid cells, consistent with the expression profile of endogenous Bruton's tyrosine kinase (Btk). Notably, EµB29 end EµBtkp activity was superior in all expression models to an alternative, B-lineage targeted vector containing the EµS.CD19 enhancer/promoter. In summary, EµB29 and EµBtkp LV comprise efficient delivery platforms for gene expression in B-lineage cells. [less ▲]

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See detailDevelopment of benchmark models for the Egyptian residential buildings sector
Attia, Shady

in Applied Energy (2011)

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See detailDevelopment of benchmark models for the Egyptian residential buildings sector
Attia, Shady ULg; Evrard, Arnaud; Gratia, Elisabeth

in Applied Energy (2012), 94

The aim of this study is to develop representative simulation building energy data sets and benchmark models for the Egyptian residential sector. This study reports the results of a recent field survey ... [more ▼]

The aim of this study is to develop representative simulation building energy data sets and benchmark models for the Egyptian residential sector. This study reports the results of a recent field survey for residential apartment buildings in Egypt. Two building performance simulation models are created reflecting the average energy consumption characteristics of air-conditioned residential apartments in Alexandria, Cairo and Asyut. Aiming for future evaluation of the cost and energy affects of the new Egyptian energy standard this study established two detailed models describing the energy use profiles for air-conditioners, lighting, domestic hot water and appliances in respect to buildings layout and construction. Using EnergyPlus simulation tool the collected surveyed data was used as input for two building simulation models. The simulation models were verified against the apartment characteristic found in the survey. This paper presents details of the building models including the energy use patterns and profiles created for this study. [less ▲]

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See detailDevelopment of Bio-climatic zones in North East India
Singh, Manoj Kumar ULg; Mahapatra, Sadhan; Atreya, S.K.

in Energy & Buildings (2007), 39(12), 1250-1257

This paper represents the very basic work of bio-climatic classification of north-east India into warm and humid, cool and humid and cold and cloudy zones. This classification is extremely useful to the ... [more ▼]

This paper represents the very basic work of bio-climatic classification of north-east India into warm and humid, cool and humid and cold and cloudy zones. This classification is extremely useful to the building design for wide range of objectives such as climate responsive building design, building energy conservation, thermal comfort, out door design conditions, etc. This classification is based on the meteorological data collected over a period of more than 30 years, from Regional Meteorological Centre, Guwahati, India from 30 stations spread all over the region. Psychometric charts are prepared for each bio-climatic zone to assess the potential of solar passive design strategies for residential buildings. [less ▲]

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See detailDevelopment of biofilters to treat the pesticides wastes from spraying applications.
Pigeon, O.; de Vleeschouwer, C.; Cors, F. et al

in Communications in Agricultural and Applied Biological Sciences (2005), 70(4),

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See detailDevelopment of biological control formulations incorporating components of plant origin
Heuskin, Stéphanie ULg; Lorge, Stéphanie; Verheggen, François ULg et al

in Journée Scientifique annuelle de la Société Royale de Chimie: Jeudi 14 octobre 2010, Gembloux Agro-Bio Tech, Université de Liège, Chimie verte (2010, October 14)

Semiochemicals – informative molecules used in insect-insect or plant-insect interactions – have been considered within various integrated pest management IPM strategies. Herein, two sesquiterpenoids: E-β ... [more ▼]

Semiochemicals – informative molecules used in insect-insect or plant-insect interactions – have been considered within various integrated pest management IPM strategies. Herein, two sesquiterpenoids: E-β-farnesene and E-β-caryophyllene have been formulated for their potential properties as aphid enemies attractants. Indeed, E-β-farnesene, the alarm pheromone of many aphid species [1], has also been identified as a kairomone by attracting and inducing oviposition of aphid predators (Episyrphus balteatus De Geer (Diptera: Syrphidae)) [2-5] and by attracting aphid parasitoids (Aphidius ervi Haliday (Hymenoptera: Braconidae)) [6, 7]. E-β-caryophyllene was identified as a potential component of the aggregation pheromone of the Asian ladybird, Harmonia axyridis Pallas [8, 9]. The two products have been purified from essential oils of Matricaria chamomilla L. (Asteraceae) and Nepeta cataria L. (Lamiaceae) [10, 11]. Natural and biodegradable slow-release devices have been investigated in order to deliver these molecules. Moreover, due to their sensitivity to oxidation, the sesquiterpenes needed to be protected. For this purpose, alginate (hydrophilic matrix with low oxygen permeability) was used as polymer for the formulations: the main objective was to hold and deliver semiochemical substances in a controlled way. Consequently, a careful selection of alginates was realised for encapsulation. Formulated beads showed different structural and encapsulation properties depending on factors such as polymer concentration, ionic strength, type and concentration of cross-linker ion,... The gel structure influenced diffusion properties. Alginate formulations were characterized by texturometry and by confocal microscopy in order to observe the distribution of semiochemicals in alginate network. The last step consisted in studying release rate of semiochemicals in laboratory-controlled conditions by adapted trapping and Fast-GC procedures [10,11]. [less ▲]

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See detailDevelopment of bioprocesses based in biphasic media involving the yeast Yarrowia lipolytica
Gomes, N.; Gonçalves, C.; Lopes, M. et al

in Ferreira, E.C.; Mota, M. (Eds.) Proceedings of the International Chemical and Biological Engineering Conference (2008)

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See detailDevelopment of bovine herpesvirus 4 as an expression vector using bacterial artificial chromosome cloning.
Gillet, Laurent ULg; Daix, V.; Donofrio, G. et al

in Journal of General Virology (The) (2005), 86(Pt 4), 907-17

Several features make bovine herpesvirus 4 (BoHV-4) attractive as a backbone for use as a viral expression vector and/or as a model to study gammaherpesvirus biology. However, these developments have been ... [more ▼]

Several features make bovine herpesvirus 4 (BoHV-4) attractive as a backbone for use as a viral expression vector and/or as a model to study gammaherpesvirus biology. However, these developments have been impeded by the difficulty in manipulating its large genome using classical homologous recombination in eukaryotic cells. In the present study, the feasibility of exploiting bacterial artificial chromosome (BAC) cloning and prokaryotic recombination technology for production of BoHV-4 recombinants was explored. Firstly, the BoHV-4 genome was BAC cloned using two potential insertion sites. Both sites of insertion gave rise to BoHV-4 BAC clones stably maintained in bacteria and able to regenerate virions when transfected into permissive cells. Reconstituted virus replicated comparably to wild-type parental virus and the loxP-flanked BAC cassette was excised by growing them on permissive cells stably expressing Cre recombinase. Secondly, BoHV-4 recombinants expressing Ixodes ricinus anti-complement protein I or II (IRAC I/II) were produced using a two-step mutagenesis procedure in Escherichia coli. Both recombinants induced expression of high levels of functional IRAC molecules in the supernatant of infected cells. This study demonstrates that BAC cloning and prokaryotic recombination technology are powerful tools for the development of BoHV-4 as an expression vector and for further fundamental studies of this gammaherpesvirus. [less ▲]

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