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See detailDirect extraction of hadronic form factors from elastic-scattering data
Martynov, E.; Cudell, Jean-René ULg; Lengyel, A.

in Nuclear Physics B-Proceedings Supplements (2005, September), 146

Non-forward elastic hadron-scattering data are collected and analysed within the Regge approach. Through an analysis of the data in small bins in t, we have directly extracted the pomeron trajectory and ... [more ▼]

Non-forward elastic hadron-scattering data are collected and analysed within the Regge approach. Through an analysis of the data in small bins in t, we have directly extracted the pomeron trajectory and the hadronic form factors (or reggeon couplings). We found higher values than usually used for the intercept and for the slope of the soft pomeron trajectory. The presence of zeros in t for the effective hadronic form factors is emphasised. [less ▲]

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See detailDirect identification of bacteria from BacT/ALERT anaerobic positive blood cultures by MALDI-TOF MS: MALDI Sepsityper kit versus an in-house saponin method for bacterial extraction.
MEEX, Cécile ULg; Neuville, Florence; DESCY, Julie ULg et al

in Journal of Medical Microbiology (2012), 61

In cases of bacteraemia, a rapid species identification of the causal agent directly from positive blood culture broths could assist clinicians in the timely targeting of empirical antimicrobial therapy ... [more ▼]

In cases of bacteraemia, a rapid species identification of the causal agent directly from positive blood culture broths could assist clinicians in the timely targeting of empirical antimicrobial therapy. For this purpose, we evaluated the direct identification of micro-organisms from BacT/ALERT (bioMérieux) anaerobic positive blood cultures without charcoal using the Microflex matrix-assisted laser desorption/ionization (MALDI) time of flight MS (Bruker), after bacterial extraction by using two different methods: the MALDI Sepsityper kit (Bruker) and an in-house saponin lysis method. Bruker's recommended criteria for identification were expanded in this study, with acceptance of the species identification when the first three results with the best matches with the MALDI Biotyper database were identical, whatever the scores were. In total, 107 monobacterial cultures and six polymicrobial cultures from 77 different patients were included in this study. Among monomicrobial cultures, we identified up to the species level 67 and 66 % of bacteria with the MALDI Sepsityper kit and the saponin method, respectively. There was no significant difference between the two extraction methods. The direct species identification was particularly inconclusive for Gram-positive bacteria, as only 58 and 52 % of them were identified to the species level with the MALDI Sepsityper kit and the saponin method, respectively. Results for Gram-negative bacilli were better, with 82.5 and 90 % of correct identification to the species level with the MALDI Sepsityper kit and the saponin method, respectively. No misidentifications were given by the direct procedures when compared with identifications provided by the conventional method. Concerning the six polymicrobial blood cultures, whatever the extraction method used, a correct direct identification was only provided for one of the isolated bacteria on solid medium in all cases. The analysis of the time-to-result demonstrated a reduction in the turnaround time for identification ranging from 1 h 06 min to 24 h 44 min, when performing the blood culture direct identification in comparison with the conventional method, whatever the extraction method. [less ▲]

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See detailDirect imaging of Earth-like planets: why we care about exozodis
Absil, Olivier ULg; Defrère, D.; Roberge, A. et al

in Danchi, W. C.; Delplancke, F.; Rajagopal, J. K. (Eds.) Optical and Infrared Interferometry II (2010, July)

The presence of large amounts of exozodiacal dust around nearby main sequence stars is considered as a potential threat for the direct detection of Earth-like exoplanets (exoEarths) with future space ... [more ▼]

The presence of large amounts of exozodiacal dust around nearby main sequence stars is considered as a potential threat for the direct detection of Earth-like exoplanets (exoEarths) with future space-based coronagraphic and interferometric missions. In this paper, we estimate the amount of exozodiacal light that can be tolerated around various stellar types without jeopardizing the detection of exoEarths with a space-based visible coronagraph or a free-flying mid-infrared interferometer. We also address the possible effects of resonant structures in exozodiacal disks. We then review the sensitivity of current ground-based interferometric instruments to exozodiacal disks, based on classical visibility measurements and on the nulling technique. We show that the current instrumental performances are not sufficient to help prepare future exoEarth imaging missions, and discuss how new groundor space-based instruments could improve the current sensitivity to exozodiacal disks down to a suitable level. [less ▲]

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See detailDirect imaging of extra-solar planets in star forming regions. Lessons learned from a false positive around IM Lup.
Mawet, Dimitri; Absil, Olivier ULg; Riaud, Pierre et al

in Astronomy and Astrophysics (2012), 544

Context. Most planet imagers consist of ground-based adaptive optics coronagraphic cameras which are currently limited in contrast, sensitivity and astrometric precision, but advantageously observe in the ... [more ▼]

Context. Most planet imagers consist of ground-based adaptive optics coronagraphic cameras which are currently limited in contrast, sensitivity and astrometric precision, but advantageously observe in the near-infrared window (1 - 5 µm). Because of these practical limitations, our current observational aim at detecting and characterizing planets puts heavy constraints on target selection, observing strategies, data reduction, and follow-up. Most surveys so far have thus targeted very young systems (1 - 100Myr) to catch the putative remnant thermal radiation of giant planets, which peaks in the near-infrared. They also favor systems in the solar neighborhood (d < 80 pc), which eases angular resolution requirements but also ensures a good knowledge of the distance and proper motion, which are critical to secure the planet status, and enable any subsequent characterization. Aims. Because of their youth, it is very tempting to target the nearby star forming regions, which are typically twice as far as the bulk of objects usually combed for planets by direct imaging. Probing these very interesting reservoirs in practice sets additional constraints that we review in this paper by presenting the planet search that we initiated in 2008 around the disk-bearing T Tauri star IM Lup, which is part of the Lupus star forming region (140-190 pc). Methods. We show and discuss why age determination, the choice of evolutionary model for both the central star and the planet, precise knowledge of the host star proper motion, relative or absolute (between different instruments) astrometric accuracy (including plate scale calibration), and patience are the key ingredients for exoplanet searches around more distant young stars. Results. Unfortunately, most of the time, precision and perseverance are not paying off: we discovered a candidate companion around IM Lup in 2008, which we report here to be a false positive event. We nevertheless review in details the lessons learned from our endeavor, and additionally present the best detection limits ever calculated for IM Lup. We also accessorily report on the successful use of innovative data reduction techniques, such as the damped-LOCI and iterative roll subtraction. [less ▲]

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See detailDirect imaging, photometry and spectroscopy of gravitationally lensed quasars
Ricci, Davide ULg

Doctoral thesis (2012)

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See detailDirect Inclusion of Proximity-Effect Losses in Two-Dimensional Time-Domain Finite-Element Simulation of Electrical Machines
Gyselinck, Johan; Dular, Patrick ULg; Geuzaine, Christophe ULg et al

in Proceedings of the 8th International Symposium on Electric and Magnetic Fields (EMF2009) (2009)

This paper deals with eddy-current effects in the distributed winding of electrical machines, and in particular with the proximity effect and the associated losses. A previously proposed homogenization ... [more ▼]

This paper deals with eddy-current effects in the distributed winding of electrical machines, and in particular with the proximity effect and the associated losses. A previously proposed homogenization method for windings in two-dimensional (2D) time-domain finite-element (FE) models is shown to be applicable without additional computational cost, producing a precise estimate of the instantaneous proximity-effect losses. The method is illustrated by considering the conductors in a single stator slot of a 3kW induction motor. The brute-force model, with fine discretisation of each conductor, and the homogenized model yield macroscopic results that are very close to each other. [less ▲]

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See detailDirect induction of burst firing by SK channel blockade in serotonergic neurons in vivo
Rouchet, Nathalie; Waroux, Olivier ULg; Moreau, Jacqueline ULg et al

Scientific conference (2007, November 04)

Small conductance calcium-activated potassium channels (SK channels) are widely expressed throughout the central nervous system and underlie the medium afterhyperpolarization following a single or a train ... [more ▼]

Small conductance calcium-activated potassium channels (SK channels) are widely expressed throughout the central nervous system and underlie the medium afterhyperpolarization following a single or a train of action potentials. It has been shown that they are involved in the regulation of the excitability and the firing pattern of several types of neurons. In vivo, serotonergic (5-HT) neurons of the dorsal raphe nucleus usually show a tonic pattern of discharge, but they can also display repetitive burst firing activity, usually involving doublets of closely spaced (< 20 ms) action potentials. It has been shown that burst firing is correlated with an increase in transmitter release and postsynaptic effects (Gartside et al., Neuroscience, 98, 295-300, 2000). We hypothesized that SK channels modulate the firing pattern of 5-HT neurons. In a preliminary study, extracellular single-cell recordings combined with iontophoresis showed that UCL1684, a water soluble SK blocker (200 µM), significantly increased the % of spikes produced in bursts in 60% of presumed serotonergic neurons in the anesthetized rat. We confirm here this observation by demonstrating that UCL1684 significantly increased the production of doublets in 17 out of 25 serotonergic neurons. In order to explore whether a GABAergic input was involved in this effect, additional experiments were performed in the presence of the specific GABAA antagonist SR 95531. In these conditions, 50 % (5 out of 10) of serotonergic neurons showed an increase in the production of doublets when UCL 1684 was applied (p = 0.31 vs control), suggesting that a GABAergic input is not implicated in the regulation of the firing pattern of 5-HT neurons by the SK blocker. Finally, the effect of SK channel blockade was explored in vitro in slices. Bath application of the SK blocker apamin (300 nM) did not induce bursting in 15 out of 18 neurons (p < 0.001 vs in vivo control conditions), although it did increase the coefficient of variation of the interspike intervals.Taken together, our results suggest that SK blockade induces burst firing in a majority of dorsal raphe serotonergic neurons. This effect does not involve GABAergic interneurons, but requires an input that is only present in vivo. [less ▲]

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See detailDirect inhibition of the DNA-binding activity of POU transcription factors Pit-1 and Brn-3 by selective binding of a phenyl-furan-benzimidazole dication.
Peixoto, Paul ULg; Liu, Yang; Depauw, Sabine et al

in Nucleic Acids Research (2008), 36(10), 3341-53

The development of small molecules to control gene expression could be the spearhead of future-targeted therapeutic approaches in multiple pathologies. Among heterocyclic dications developed with this aim ... [more ▼]

The development of small molecules to control gene expression could be the spearhead of future-targeted therapeutic approaches in multiple pathologies. Among heterocyclic dications developed with this aim, a phenyl-furan-benzimidazole dication DB293 binds AT-rich sites as a monomer and 5'-ATGA sequence as a stacked dimer, both in the minor groove. Here, we used a protein/DNA array approach to evaluate the ability of DB293 to specifically inhibit transcription factors DNA-binding in a single-step, competitive mode. DB293 inhibits two POU-domain transcription factors Pit-1 and Brn-3 but not IRF-1, despite the presence of an ATGA and AT-rich sites within all three consensus sequences. EMSA, DNase I footprinting and surface-plasmon-resonance experiments determined the precise binding site, affinity and stoichiometry of DB293 interaction to the consensus targets. Binding of DB293 occurred as a cooperative dimer on the ATGA part of Brn-3 site but as two monomers on AT-rich sites of IRF-1 sequence. For Pit-1 site, ATGA or AT-rich mutated sequences identified the contribution of both sites for DB293 recognition. In conclusion, DB293 is a strong inhibitor of two POU-domain transcription factors through a cooperative binding to ATGA. These findings are the first to show that heterocyclic dications can inhibit major groove transcription factors and they open the door to the control of transcription factors activity by those compounds. [less ▲]

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See detailDirect measurement of static chest wall compliance in unsedated calves
Desmecht, Daniel ULg; Rollin, Frédéric ULg; Amory, Hélène ULg et al

in Proceedings of the 8th Comparative Respiratory Society Meeting (1989)

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See detailDirect measurements of interactions between hydrophobically anchored strongly charged polyelectrolyte brushes
Abraham, T.; Giasson, S.; Gohy, Jean-François et al

in Langmuir (2000), 16(9), 4286-4292

We investigated the nature and the range of interactions between negatively charged polyelectrolyte-coated surfaces as a function of salt concentration using the surface forces apparatus. The measured ... [more ▼]

We investigated the nature and the range of interactions between negatively charged polyelectrolyte-coated surfaces as a function of salt concentration using the surface forces apparatus. The measured force profiles (interaction forces versus separation distance) are purely repulsive and show long-range electrostatic and short-range steric interactions. The measured range of interaction in salt-free as well as in low ionic strengths extends well beyond the contour length of the polyelectrolyte chain. It is shown that the interaction range between the ionic brush layers and the grafting density depend on the ionic strength of the solution. In salt-free solution, the counterions associated with polyelectrolyte cause the chains to stretch and give rise to long-range double-layer electrostatic repulsions between the opposing chains. When salt is added to the system, the electrostatic interactions are partially screened and the polymer chain regains its flexibility and therefore the range of interactions is reduced. The measured total range of interaction exhibits relatively weaker dependence on the salt concentration. We find that our force−distance profiles with added salt in a compressed regime can be very well described by the Pincus scaling model. [less ▲]

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See detailDirect Modelling of Unit Operations on Molecular Level
Babic, D; Pfennig, Andreas ULg

Conference (2006)

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See detailDirect multiple-point geostatistical simulation of edge properties for modeling thin irregularly-shaped surfaces
Huysmans, Marijke; Dassargues, Alain ULg

in Mathematical Geosciences (2011), 43

Thin irregularly-shaped surfaces such as clay drapes often have a major control on flow and transport in heterogeneous porous media. Clay drapes are often complex curvilinear 3-dimensional surfaces and ... [more ▼]

Thin irregularly-shaped surfaces such as clay drapes often have a major control on flow and transport in heterogeneous porous media. Clay drapes are often complex curvilinear 3-dimensional surfaces and display a very complex spatial distribution. Variogram-based stochastic approaches are often also not able to describe the spatial distribution of clay drapes since complex, curvilinear, continuous and interconnected structures cannot be characterized using only two-point statistics. Multiple-point geostatistics aims to overcome the limitations of the variogram. The premise of multiple-point geostatistics is to move beyond two-point correlations between variables and to obtain (cross) correlation moments at three or more locations at a time using "training images" to characterize the patterns of geological heterogeneity. Multiple-point geostatistics can reproduce thin irregularly-shaped surfaces such as clay drapes but is often computationally very intensive. This paper describes and applies a methodology to simulate thin irregularly-shaped surfaces with a smaller CPU and RAM demand than the conventional multiple-point statistical methods. The proposed method uses edge properties for indicating the presence of thin irregularly-shaped surfaces. This method allows directly simulating edge properties instead of pixel properties to make it possible to perform multiple-point geostatistical simulations with a larger cell size and thus a smaller computation time and memory demand. [less ▲]

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See detailDirect multiple-point geostatistical simulation of edge properties for modeling thin irregularly-shaped surfaces
Huysmans, Marijke; Dassargues, Alain ULg

in Cockx, L.; Van Meirvenne, M.; Bogaert, P. (Eds.) et al 8th International Conference On Geostatistics for Environmental Applications, GeoENV’2010 (2010, September)

Thin irregularly-shaped surfaces such as clay drapes often have a major control on flow and transport in heterogeneous porous media. Clay drapes are often complex curvilinear 3-dimensional surfaces and ... [more ▼]

Thin irregularly-shaped surfaces such as clay drapes often have a major control on flow and transport in heterogeneous porous media. Clay drapes are often complex curvilinear 3-dimensional surfaces and display a very complex spatial distribution. Variogram-based stochastic approaches are often also not able to describe the spatial distribution of clay drapes since complex, curvilinear, continuous and interconnected structures cannot be characterized using only two-point statistics. Multiple-point geostatistics aims to overcome the limitations of the variogram. The premise of multiple-point geostatistics is to move beyond two-point correlations between variables and to obtain (cross) correlation moments at three or more locations at a time using "training images" to characterize the patterns of geological heterogeneity. Multiple-point geostatistics is able to reproduce thin irregularly-shaped surfaces such as clay drapes but is often computationally intensive. To capture the thin surfaces, a small grid cell size should be adopted for the training image. This results in large training images and a large search template size and thus a large CPU and RAM demand (Huysmans and Dassargues, 2009). [less ▲]

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See detailDirect n.m.r. evidence for substrate-induced conformational changes in a beta-lactamase.
Jamin, M.; Damblon, Christian ULg; Bauduin-Misselyn, A. M. et al

in Biochemical Journal (1994), 301 ( Pt 1)

Cefoxitin and other beta-lactam antibiotics with a methoxy group on the alpha-face behave as very poor substrates of the Bacillus licheniformis beta-lactamase. The kinetic properties of the enzyme ... [more ▼]

Cefoxitin and other beta-lactam antibiotics with a methoxy group on the alpha-face behave as very poor substrates of the Bacillus licheniformis beta-lactamase. The kinetic properties of the enzyme-cefoxitin system made it theoretically suitable for a detailed structural study of the acyl-enzyme. Unfortunately, soaking the crystals in cefoxitin solution did not allow detection of a crystalline acyl-enzyme complex. In contrast, direct observation by n.m.r. of the stable acyl-enzyme formed with cefoxitin and moxalactam indicated clear modifications of the enzyme structure, which were reflected in the aromatic and high-field methyl regions of the spectrum. The return to the initial free enzyme spectrum was concomitant with the hydrolysis of the acyl-enzyme, the process being slow enough to allow multidimensional n.m.r. experiments. [less ▲]

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See detailDirect nano-in-micropatterning of TiO2 thin layers and TiO2/Pt nanoelectrode arrays by deep X-ray lithography
Faustini, M.; Marmiroli, B.; Malfatti, L. et al

in Journal of Materials Chemistry (2011), 21

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See detailDirect NMR evidence for a local resriction in the segmental chain mobility of a model ionomer
Vanhoorne, Pierre; Jérôme, Robert ULg; Teyssié, Philippe et al

in Macromolecules (1994), 27(9), 2548-2552

To probe the local mobility of the chain segments directly attached to ionic multiplets, two model ionomers have been synthesized. The first one is a carboxylato-telechelic deuterated polystyrene in which ... [more ▼]

To probe the local mobility of the chain segments directly attached to ionic multiplets, two model ionomers have been synthesized. The first one is a carboxylato-telechelic deuterated polystyrene in which the chain segments bearing the sodium carboxylate end groups have been selectively protonated; the second one has a protonated polystyrene backbone terminated at both ends with a deuterated polystyrene segment bearing the sodium carboxylate groups. Both polymers have been studied by high-resolution solid-state C-13 NMR. Line-width measurements of the protonated units have clearly shown that the dipolar interactions of the sodium carboxylate end groups are responsible for a mobility restriction of the chain end segments. This experiment is thought to be the first direct evidence for the model recently proposed by Eisenberg et al. for ionomer morphology according to which the ionic multiplets are surrounded with a shell of constrained polymer segments. [less ▲]

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See detailDirect Numerical Simulation of Mixed Convection in Turbulent Channel Flow: On the Reynolds number dependency of momentum and heat transfer under unstable stratification
Sid, Samir ULg; Dubief, Yves; Terrapon, Vincent ULg

Conference (2015, May 27)

Direct numerical simulations of unstably stratified turbulent channel flow have been performed in order to investigate the Reynolds number effect on mixed convection. Six different cases are considered ... [more ▼]

Direct numerical simulations of unstably stratified turbulent channel flow have been performed in order to investigate the Reynolds number effect on mixed convection. Six different cases are considered with friction Reynolds number Re_\tau =180 and 395 and friction Richardson number Ri_\tau = 0, 100 and 1000. It is shown that both friction coefficient and Nusselt number increase under unstable stratification for a sufficiently large Richardson number. At low Richardson number, the friction coefficient can either increase or decrease depending on the Reynolds number. The drag reduction is associated with an increase of mean velocity due to an enhanced dissipation of Reynolds shear stress by pressure strain in the buffer region. The breakdown of the Reynolds analogy is demonstrated as the turbulent Prandtl number exhibits a non-constant behavior due to buoyancy. [less ▲]

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See detailDirect observation of microdomain morphology in "all-acrylic" thermoplastic elastomers synthesized via living radical polymerization
Leclère, Philippe; Moineau, Georges; Minet, Michaël et al

in Langmuir (1999), 15(11), 3915-3919

We investigate the microscopic morphology of thin films of symmetric triblock copolymers synthesized via a two-step “living” radical polymerization of n-butylacrylate and methyl methacrylate. These ... [more ▼]

We investigate the microscopic morphology of thin films of symmetric triblock copolymers synthesized via a two-step “living” radical polymerization of n-butylacrylate and methyl methacrylate. These copolymers with low poly(methyl methacrylate) contents constitute a new class of potential thermoplastic elastomers with higher service temperature and oxidation resistance compared to conventional polydiene−polystyrene-based thermoplastic elastomers. The straightforward synthetic pathway allows for strict control of molecular weight, molecular-weight distribution, and composition. The presence in the copolymer of immiscible segments covalently bound to each other leads to phase separation on the nanometer scale. Regular organization of the phase-separated nanodomains is observed in real space by scanning force microscopy. The data point to a strong contrast in the local mechanical properties, corresponding to the microphase morphology. Cylinders of the minority phase are found to orient perpendicular to the surface, because of the surface energy difference between the constituents. Lamellae are also arranged perpendicular to the surface, in contrast to what is usually observed in block copolymers. This particular orientation is thought to result from the symmetric character of these triblock systems, with the outer blocks more polar than the central sequence. [less ▲]

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