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See detailEffects Of Commercial Fatty-Acids On Cutinase Release By Ascochyta-Pisi
Nasraoui, B.; Lepoivre, Philippe ULg; Semal, J.

in Journal of Phytopathology-Phytopathologische Zeitschrift (1992), 136(3),

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See detailEffects of compensatory growth on animal performance and plasma metabolites in growing fattening bulls
Hornick, Jean-Luc ULg; Van Eenaeme, Christian ULg; Clinquart, Antoine ULg et al

in Proceedings of the 44th Annual Meeting of E.A.A.P. (1993)

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See detailEffects of compression on human subchondral osteoblast metabolism
Kesteloot, Frédéric ULg; Gabay, Odile; Msika, Philippe et al

Poster (2009, May 24)

Introduction. Recent data showed that subchondral bone plays an important role in osteoarthritis (OA). Metabolic and morphologic modifications in this tissue contribute to the degradation of the ... [more ▼]

Introduction. Recent data showed that subchondral bone plays an important role in osteoarthritis (OA). Metabolic and morphologic modifications in this tissue contribute to the degradation of the overlaying cartilage. It was suggested that abnormal mechanical pressure exerted onto the articulation was responsible to these changes. Here, we evaluated the effects of compression on osteoblasts from subchondral bone. Method. Osteoblasts were isolated from sclerotic (SC) or non-sclerotic (NSC) areas of human OA subchondral bone. After 28 days, osteoblasts were surrounded by their matrix. This osteoblasts-containing membrane was then placed onto a Biopress Flexercell plate and submitted to a 4h 1.67 MPa compression (1 Hz). Expression of IL-6, IL-8, COX-2, VEGF, IGF-1, OPG and RANKL was evaluated by RT-PCR. IL-6, IL-8 and PGE2 were quantified by ELISA. Results. Basal IL-6, VEGF, COX-2, IGF-1 and RANKL mRNA levels were significantly increased in SC osteoblasts as compared to NSC. By contrast, SC osteoblasts expressed less OPG than those from NSC areas. Compressions induced the expression of genes coding for IL-6, IL-8, COX-2, IGF-1, VEGF and RANKL but decreased the expression of OPG in NSC osteoblasts (p<0.01). Interestingly, compressed NSC osteoblasts expressed similar levels of these genes than SC osteoblasts. Conclusions. We show that our model of compression can induce in NSC osteoblasts a phenotype similar to this observed in sclerotic areas. Moreover, SC osteoblasts are less sensitive to mechanical stimuli than NSC osteoblasts. These results clarify the role of compression in the pathogenesis of subchondral bone sclerosis and allow new perspectives of research in this field. [less ▲]

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See detailEffects of concentrate level on milk production and traffic of grazing cows milked by a mobile automatic milking system on pasture
Lessire, Françoise ULg; Hornick, Jean-Luc ULg; Dufrasne, Isabelle ULg

in Hopkins, Alan; Collins, Rosemary; Fraser, Marieacia (Eds.) et al EGF at 50: The future of European Grasslands (2014, September 10)

Cows milked by an automatic milking system in pastures were assigned in 2 groups receiving different amounts of concentrates (2.1 kg vs 4.1 kg). The effect of concentrates’ level on milk yield (MY) and ... [more ▼]

Cows milked by an automatic milking system in pastures were assigned in 2 groups receiving different amounts of concentrates (2.1 kg vs 4.1 kg). The effect of concentrates’ level on milk yield (MY) and returns to the robot was assessed. Concentrates’ level had a positive influence on daily milk production over the grazing period as cows of low concentrates group produced 21.43 ± 0.62 kg compared with 24.33 ± 0.62 kg in high concentrates group. However this effect was modulated subsequently to grass quality and availability. Regarding daily voluntary returns to the robot, high concentrates group showed higher frequency (3.66 ± 0.05, compared with 3.22 ± 0.04 in low concentrates group) demonstrating positive impact of complement distribution on cows’ traffic. [less ▲]

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See detailEffects of concentrate level on milk production and traffic of grazing cows milked by a mobile automatic milking system on pasture
Lessire, Françoise ULg; Hornick, Jean-Luc ULg; Dufrasne, Isabelle ULg

in Hopkins, Alan; Collins, Rosemary; Fraser, Marieacia (Eds.) et al EGF at 50: The future of European Grasslands (2014, September 10)

Cows milked by an automatic milking system in pastures were assigned in 2 groups receiving different amounts of concentrates (2.1 kg vs 4.1 kg). The effect of concentrates’ level on milk yield (MY) and ... [more ▼]

Cows milked by an automatic milking system in pastures were assigned in 2 groups receiving different amounts of concentrates (2.1 kg vs 4.1 kg). The effect of concentrates’ level on milk yield (MY) and returns to the robot was assessed. Concentrates’ level had a positive influence on daily milk production over the grazing period as cows of low concentrates group produced 21.43 ± 0.62 kg compared with 24.33 ± 0.62 kg in high concentrates group. However this effect was modulated subsequently to grass quality and availability. Regarding daily voluntary returns to the robot, high concentrates group showed higher frequency (3.66 ± 0.05, compared with 3.22 ± 0.04 in low concentrates group) demonstrating positive impact of complement distribution on cows’ traffic. [less ▲]

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See detailEffects of concentrate level on milk production and traffic of grazing cows milked by a mobile automatic milking system on pasture
Lessire, Françoise ULg; Hornick, Jean-Luc ULg; Dufrasne, Isabelle ULg

in Hopkins, Alan; Collins, Rosemary; Fraser, Marieacia (Eds.) et al EGF at 50: The future of European Grasslands (2014, September 10)

Cows milked by an automatic milking system in pastures were assigned in 2 groups receiving different amounts of concentrates (2.1 kg vs 4.1 kg). The effect of concentrates’ level on milk yield (MY) and ... [more ▼]

Cows milked by an automatic milking system in pastures were assigned in 2 groups receiving different amounts of concentrates (2.1 kg vs 4.1 kg). The effect of concentrates’ level on milk yield (MY) and returns to the robot was assessed. Concentrates’ level had a positive influence on daily milk production over the grazing period as cows of low concentrates group produced 21.43 ± 0.62 kg compared with 24.33 ± 0.62 kg in high concentrates group. However this effect was modulated subsequently to grass quality and availability. Regarding daily voluntary returns to the robot, high concentrates group showed higher frequency (3.66 ± 0.05, compared with 3.22 ± 0.04 in low concentrates group) demonstrating positive impact of complement distribution on cows’ traffic. [less ▲]

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See detailEffects of consolidation of procedural motor memory traces on slow and fast spindles
Barakat, M; Doyon, J; Debas, K et al

Poster (2009, April)

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See detailEffects of constitutively active GTPases on fibroblast behavior.
Zhang, Z.-G.; Lambert, Charles ULg; Servotte, S. et al

in Cellular and Molecular Life Sciences : CMLS (2006), 63(1), 82-91

The GTP-binding proteins RhoA, Cdc42 and Rac1 regulate the organization and turnover of the cytoskeleton and cell-matrix adhesions, structures bridging cells to their support, and translating forces ... [more ▼]

The GTP-binding proteins RhoA, Cdc42 and Rac1 regulate the organization and turnover of the cytoskeleton and cell-matrix adhesions, structures bridging cells to their support, and translating forces, external or generated within the cell. To investigate the specific requirements of Rho GTPases for biomechanical activities of clonal cell populations, we compared side-by-side stable lines of human fibroblasts expressing constitutively active (CA) RhoA, Cdc42 or Rac1. There was no marked effect of any CA GTPase on cell adhesion to different extracellular matrix proteins. Cell spreading was CA Rho GTPase specific and independent of the extracellular matrix proteins allowing adhesion. Mechanical properties were dramatically restricted by CA RhoA on bi- and in tri-dimensional surroundings, were boosted by CA Rac1 on bi-dimensional surroundings only, and were not or marginally affected by CA Cdc42. In conclusion, the action of Rho GTPases appears to depend on the task cells are performing. [less ▲]

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See detailEffects of copper sulfate given alone or with alpha-MSH and L-tyrosine on B16 melanoma cells cultured in serum-free media.
Gillet, Marie-Claire ULg; wang, François; Simonon, A. et al

in Archives of Biology (1987), 98(3), 327-39

This study shows that copper sulphate (0.1mM) has antiproliferative effects in B16 melanoma cells cutured in various serum-free media.

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See detailEffects of COX-2 inhibitors on ROS produced by Chlamydia pneumoniae-primed human promonocytic cells (THP-1)
Mouithys-Mickalad, Ange ULg; Deby, Ginette ULg; Dogné, Jean-Michel ULg et al

in Biochemical and Biophysical Research Communications (2004), 325(4), 1122-1130

Chronic inflammation through foam cells and macrophages is important in atherosclerosis development, and can be considered as therapeutic targets. Cyclooxygenase and NADPH-oxidase were expressed within ... [more ▼]

Chronic inflammation through foam cells and macrophages is important in atherosclerosis development, and can be considered as therapeutic targets. Cyclooxygenase and NADPH-oxidase were expressed within atherosclerotic lesions. Reactive oxygen species produced by NADPH oxidase were found to trigger the cyclooxygenase-2 expression. The effects of preferential COX-2 inhibitors on ROS produced by Chlamydia-primed human monocytes (THP-1 cells) were evaluated by fluorescence, chemiluminescence, oxymetry, and EPR spin trapping. Fluorescence assays showed an increased production of ROS with Chlamydia versus cells primed by 10(-8) M PMA. COX-2 inhibitors inhibited in a dose-dependent manner the luminol-enhanced CL while ibuprofen and diclofenac increased the chemiluminescence response. By EPR spin trapping, COX-2 inhibitors, ibuprofen, and diclofenac, exhibited a dose-dependent inhibiting effect (10 and 100 muM) on the EPR signal appearance. Our cell model combining EPR, chemiluminescence, and oxymetry appeared relevant to study the modulating effects of preferential COX-2 inhibitors on the cell oxidant activity and chronic inflammatory diseases. (C) 2004 Elsevier Inc. All rights reserved. [less ▲]

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See detailEffects of cromakalim analogues on rat pancreatic B-cells, rat aorta rings and rat uterus. Study of their mechanism of action as potassium channel activators
Sebille, S.; Boverie, S.; Becker, B. et al

Poster (2003, November 22)

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See detailEffects of cromakalim analogues on rat pancreatic B-cells, rat aorta rings and rat uterus. Study of their mechanism of action as potassium channel activators
Sebille, S.; Boverie, S.; Becker, B. et al

in Fundamental & Clinical Pharmacology (2004)

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See detailEffects of crops on solute transport in undisturbed soil
Garré, Sarah ULg; Javaux, Mathieu; Vanderborght, Jan

Poster (2009)

Models predicting movement of surface applied chemicals incorporate knowledge on the water velocity field and moisture content distribution. Although the influence of root water uptake on solute transport ... [more ▼]

Models predicting movement of surface applied chemicals incorporate knowledge on the water velocity field and moisture content distribution. Although the influence of root water uptake on solute transport is commonly recognized as important, it has been studied sparsely. Yet, plants may take up a large part of the infiltrating water, thereby influencing the water flow pattern in the soil and concurrently solute transport processes. For this reason, experiments are required to investigate the relationship between plant root water uptake and flow field variability. The role of root water uptake on solute transport will be elucidated in two undisturbed soil columns. During three consecutive experiments, the influence of growing barley on tracer movement through a silty soil in two lysimeters will be followed. At the first stage, an inert tracer is put on the two bare lysimeters and leached with constant irrigation. As steady-state flow can be assumed, it is possible to follow the tracer movement in the column by ERT and to identify regions of preferential flow and solute transport parameters. During the second experiment, the tracer will be applied to mature barley grown in the lysimeters. Combining the information about the water content obtained with TDR with the relation between water content, soil solution salinity and bulk electrical conductivity, the soil solution salinity distribution can be derived from images of bulk electrical conductivity obtained with ERT. Root growth will be monitored using a minirhizotron. By comparing the transport parameters obtained after these two experiments, the effect of root water on the transport process can be quantified. When the columns are washed out and the barley is harvested, the third phase will be carried out under the same steady state flow conditions as in the first experiment to investigate the effect of dead roots on soil structure. [less ▲]

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See detailEffects of culture conditions on the synthesis of human chorionic gonadotropin by placental organ cultures.
Huot, R. I.; Foidart, Jean-Michel ULg; Stromberg, K.

in In Vitro (1979), 15(7), 497-502

Culture conditions for maintaining first trimester human placenta in organ culture, which enhance the secretion of human chorionic gonadotropin (hCG), are described. Nutrient medium, oxygen tension and ... [more ▼]

Culture conditions for maintaining first trimester human placenta in organ culture, which enhance the secretion of human chorionic gonadotropin (hCG), are described. Nutrient medium, oxygen tension and Gelfoam support matrix influence the synthesis of hCG by these cultures. Placental tissue remained viable for the duration of experiments (12 days) as judged by the incorporation of tritiated thymidine into DNA and the lack of release of incorporated [125I]iododeoxyuridine. Optimal conditions for hCG synthesis in placental organ culture included an atmosphere of 95% air and 5% Co2 (approximately 20% O2), CMRL 1066 medium containing fetal human or bovine serum, insulin, hydrocortisone and retinal acetate. Multiple pieces of placenta could be cultured in the same dish with an additive effect on hCG secretion. The functional responsiveness of these placental cultures was demonstrated by modulation of hCG synthesis with theophylline and 3'5' dibutyryl cyclic AMP. [less ▲]

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See detailEffects of curing sodium nitrite additive and natural meat fat on growth control of Listeria monocytogenes by the bacteriocin-producing Lactobacillus curvatus strain CWBI-B28.
Kouakou, P.; Ghalfi, H.; Destain, Jacqueline ULg et al

in Food Microbiology (2009), 26

In realistic model meat systems, the separate and combined effects of fat content and sodium nitrite on the antilisterial activity of the bacteriocin of Lactobacillus curvatus CWBI-B28 were studied. In ... [more ▼]

In realistic model meat systems, the separate and combined effects of fat content and sodium nitrite on the antilisterial activity of the bacteriocin of Lactobacillus curvatus CWBI-B28 were studied. In laboratory fermentations where Listeria monocytogenes was co-cultured at 4 C with bacteriocin-producing CWBIB28 in lean pork meat (fat content: 13%) without added nitrite, a strong antilisterial effect was observed after one week. The effect was maintained for an additional week, after which a slight and very gradual rebound was observed. Both added nitrite (20 ppm) and a high-fat content (43%) were found to antagonise this antilisterial effect, the Listeria cfu count reached after six weeks being 200 times as high in high-fat meat with added nitrite than in lean meat without nitrite. This antagonism could not be attributed to slower growth of the bacteriocin-producing strain, since CWBI-B28 grew optimally in fatrich meat with 20 ppm sodium nitrite. Bacteriocin activity was also measured in the samples. The observed activity levels are discussed in relation to the degree of antilisterial protection conferred [less ▲]

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See detailEffects of cyanamide and acetaldehyde accumulation on the locomotor stimulant and sedative effects of ethanol in mice
Tambour, Sophie ULg; Closon, Catherine; Tirelli, Ezio ULg et al

in Behavioural Pharmacology (2007), 18(8), 777-784

Ethanol administration induces both locomotor stimulant and sedative effects depending upon blood ethanol concentrations. Recent studies in rats and mice suggest that acetaldehyde, the first product of ... [more ▼]

Ethanol administration induces both locomotor stimulant and sedative effects depending upon blood ethanol concentrations. Recent studies in rats and mice suggest that acetaldehyde, the first product of ethanol metabolism, might be involved in the expression of both the stimulant and the sedative effects of ethanol. A number of studies have used the drug cyanamide in an attempt to clarify the role of acetaldehyde in the behavioral effects of ethanol. The results of such studies are, however, difficult to interpret because cyanamide is an inhibitor of the enzymes catalase and aldehyde dehydrogenase, two enzymes with opposite effects on brain acetaldehyde concentrations. This study was aimed at clarifying the effects of cyanamide on ethanol-induced locomotor stimulant and sedative effects in Swiss mice. The locomotor stimulant effects of ethanol were measured in standard activity boxes, whereas the sedative effects of ethanol were quantified using the loss of righting reflex procedure. Cyanamide prevented the locomotor stimulant effects of 2 g/kg ethanol, although this was mainly due to a potentiation of the inhibitory effects of ethanol as evidenced by a prolongation of ethanol-induced loss of righting reflex. Additionally, 4-methylpyrazole, an inhibitor of the enzyme alcohol dehydrogenase, prevented these effects of cyanamide. It is concluded that in vivo the effects of cyanamide are predominantly due to the inhibition of the enzyme aldehyde dehydrogenase, rather than to its effects on catalase. [less ▲]

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