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See detailDetection of Angiostrongylus vasorum in the bronchoalveolar lavage fluid or faeces of coughing and healthy dogs in Belgium.
Canonne-Guibert, Morgane ULg; Roels, Elodie ULg; Caron, Yannick ULg et al

in Proceedings of the 24th Ecvim Meeting, Mainz, Germany - 4-6 September 2014 (2014, September)

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See detailDetection of anthropogenic particles in fish stomachs: an isolation method adapted to identification by Raman spectroscopy
Collard, France ULg; Gilbert, Bernard ULg; Eppe, Gauthier ULg et al

in Archives of Environmental Contamination & Toxicology (2015), 69

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See detailDetection of anti-laminin antibodies in sera by latex agglutination.
Bernard, A. M.; Foidart, Jean-Michel ULg; Mahieu, P. et al

in Clinical Chemistry (1986), 32(8), 1468-72

We describe a latex particle agglutination assay for detecting circulating antibodies against laminin, a noncollagenous glycoprotein of basement membranes. Polystyrene latex particles on which laminin has ... [more ▼]

We describe a latex particle agglutination assay for detecting circulating antibodies against laminin, a noncollagenous glycoprotein of basement membranes. Polystyrene latex particles on which laminin has been adsorbed are incubated with serum for about 25 min at 42-45 degrees C. The agglutination is then measured by counting residual unagglutinated particles. Polyethylene glycol 6000 enhances the agglutination. The assay is fully automated, yielding results in about 45 min, for 50 samples per hour. Addition of purified laminin abolishes the agglutination of laminin-coated particles in practically all positive sera. The anti-laminin antibody titers obtained by this latex immunoassay and by radioimmunoassay correlated well in 161 sera from patients with suspected or established renal diseases. The agglutination assay more frequently gave positive results for cases of glomerulonephritis with linear deposits (20/22 cases) than for glomerulonephritis with granular deposits (7/68) or glomerulonephritis with no glomerular deposits (2/13). The finding of low anti-laminin antibody titers in sera from about 15% (34/230) of the healthy subjects suggests that these autoantibodies are pathogenic only in certain circumstances. [less ▲]

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See detailDetection of antibodies against Schmallenberg virus in wild boars, Belgium, 2010-2012
Desmecht, Daniel ULg; Garigliany, Mutien-Marie ULg; Beer, Martin et al

in Lecoq, Yves (Ed.) 31th Congress of the International Union of Game Biologists (2013, August 27)

In the summer/fall of 2011, a nonspecific febrile syndrome characterized by hyperthermia and drop in milk production with occasional reports of watery diarrhea and abortion was reported among dairy cows ... [more ▼]

In the summer/fall of 2011, a nonspecific febrile syndrome characterized by hyperthermia and drop in milk production with occasional reports of watery diarrhea and abortion was reported among dairy cows on farms in northwestern Europe. Further, in November 2011, an enzootic outbreak of malformed neonates emerged in several European countries, with stillbirth and birth at term of lambs, kids and calves with neurological signs or malformations of the head, spine, or limbs. Both syndromes were associated with the presence in the blood (adults) or in the central nervous system (newborns) of a new Shamonda/Sathuperi-like orthobunyavirus, provisionally named Schmallenberg virus (SBV) after the town in Germany where the first positive clinical samples were identified. Defining as precisely as possible the host range of the newcomer is a key point to predict the outcome of the emergence of SBV disease in Europe. In this respect, it must be pointed out that orthobunyaviruses infect more animal species than those in which the foetus is damaged. Recently, serological evidence for SBV infection in wild ruminant species (Cervus elaphus and Capreolus capreolus) was reported (Linden et al., 2012). In the present study, the objective was to seek after serological evidence of SBV infection among wild boars living in a geographical area where exposure to infected insect vectors was high in 2011, as judged from the very high seroprevalence reported among cattle in that region. About 700 animals were sampled during the 2010-2012 hunting seasons. All serum samples collected during the fall of 2010 were seronegative. On the contrary, apparent seroprevalence among wild boars in 2011 was ~27% and started to decline in 2012 (~11%). Acquired immunity against the new virus was thus already very high in the wild boar populations sampled in the fall 2011, suggesting that the new virus had quickly spread throughout the region since its emergence about 250 km northeast in the late summer 2011. The drop in seroprevalence recorded in 2012 suggests that the virus was no more circulating in the region. [less ▲]

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See detailDetection of antigenic heterogeneity in feline coronavirus nucleocapsid in feline pyogranulomatous meningoencephalitis.
Poncelet, Luc; Coppens, Angélique; Peeters, Dominique ULg et al

in Veterinary Pathology (2008), 45(2)

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See detailDetection of apple chlorotic leaf spot virus and apple stem grooving virus by RT-PCR and IC-RT-PCR.
Kummert, J.; Rufflard, Gladys ULg; Colinet, D. et al

in Mededelingen van de Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen (Rijksuniversiteit te Gent) (1995), 60(2a),

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See detailDetection Of Apple Chlorotic Leaf Spot Virus Using A 5 ' Nuclease Assay With A Fluorescent 3 ' Minor Groove Binder-Dna Probe
Vendrame, Marina; Kummert, Jean; Lepoivre, Philippe ULg et al

in Journal of Virological Methods (2002), 104(1), 99-106

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See detailDetection Of Apple Stem Grooving Virus In Dormant Apple Trees With Crude Extracts As Templates For One-Step Rt-Pcr
Marinho, Vla.; Kummert, J.; Rufflard, Gladys ULg et al

in Plant Disease (1998), 82(7),

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See detailDetection of Aspergillus spp. by PCR in Bronchoalveolar Lavage Fluid
Hayette, Marie-Pierre ULg; Vaira, Dolorès ULg; Suzin, Fabrice et al

in Journal of Clinical Microbiology (2001), 39(6), 2338-2340

The usefulness of a nested PCR assay for detection of Aspergillus sp. DNA was evaluated in 177 bronchoalveolar lavage (BAL) fluid specimens. This test was accurate both to diagnose culture-negative BAL ... [more ▼]

The usefulness of a nested PCR assay for detection of Aspergillus sp. DNA was evaluated in 177 bronchoalveolar lavage (BAL) fluid specimens. This test was accurate both to diagnose culture-negative BAL fluid specimens from patients with invasive pulmonary aspergillosis and to confirm culture-positive samples. However, it did not differentiate between infection and colonization. [less ▲]

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See detailDetection of Auroral Emissions from Callisto’s Magnetic Footprint at Jupiter
Clarke, J. T.; Wannawichian, S.; Hernandez, N. et al

Poster (2011, October)

HST observations of Jupiter’s aurora in a large campaign reveal several cases where the main oval emission appeared at unusually low latitudes, making it possible to search for the first time for auroral ... [more ▼]

HST observations of Jupiter’s aurora in a large campaign reveal several cases where the main oval emission appeared at unusually low latitudes, making it possible to search for the first time for auroral emissions from the magnetic footprint of Callisto without the overlapping bright emissions from the main oval. Several cases have been found where point-source emissions have now been detected from locations consistent with Callisto’s magnetic footprint on Jupiter at a brightness of ten’s of kilo- Rayleighs. These observations confirm that there is an electrodynamic interaction between Callisto and Jupiter’s magnetospheric environment that is similar to those at Io, Europa, and Ganymede, which all have auroral footprints. The properties of the emissions and a comparison with other observations and theoretical expectations will be presented in this paper. [less ▲]

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See detailDetection Of B-Cells And T-Cells, With Lectins Or Antibodies, In Healthy And Bovine Leukemia Virus-Infected Cattle
Fossum, C.; Burny, A.; Portetelle, Daniel ULg et al

in Veterinary Immunology and Immunopathology (1988), 18(3),

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See detailDetection of bacteria in sterile body fluids
CHRISTIAENS, Geneviève ULg; HAYETTE, Marie-Pierre ULg; De Mol, Patrick ULg

Poster (1998, October)

Purpose of the study: Development of a polymerase chain reaction (PCR) method for the detection of any bacterial DNA in synovial, cerebrospinal, pleural, and peritoneal fluids. Methods: Most of bacterial ... [more ▼]

Purpose of the study: Development of a polymerase chain reaction (PCR) method for the detection of any bacterial DNA in synovial, cerebrospinal, pleural, and peritoneal fluids. Methods: Most of bacterial species possess highly conserved, multicopy 16S ribosomal RNA genes, which can be hybridized with a single set of oligonucleotide primers. Samples were processed by an extraction protocol using proteinase K, and subjected to PCR amplification using two universal bacterial primers: RW01 and DG74; then the PCR products were detected by ethidium bromide gel electrophoresis. Study: Synovial, cerebrospinal, pleural and peritoneal fluids, obtained from 32 patients were analyzed by Gram stain, culture and PCR. Results: 1. The limit of detection, determined by analyzing successive dilutions of Staphylococcus aureus and Escherichia coli cultures in sterile water, was: 1.105 cfu/100µl. 2. We obtained 9 positive samples by culture: - 7 synovial fluids: S. agalactiae, S. viridans, coagulase negative Staphylococcus (2), S. epidermidis (2), and S. aureus. - 2 pleural fluids: S. pyogenes and Enterobacter aerogenes. All were PCR positive. 3. We tested 23 culture negative samples. All were negative by PCR. Conclusion: PCR presents some interesting features: 1. Only small amount of sample is necessary (100µl). 2. The duration of the test is shorter than 8 hours. 3. PCR provides similar or eventually greater sensitivity than culture and an excellent specificity (no false-positive and no false-negative results actually observed). [less ▲]

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See detailDetection of Banana Streak Virus (BSV) by IC-PCR-ELOSA
Delanoy, M.; Jijakli, Haissam ULg; Lepoivre, Philippe ULg

Conference (2001)

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See detailDetection of biomarkers of pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Ruelle, Virginie; Elmoualij, Benaïssa ULg; Zorzi, Willy ULg et al

in Lichtfouse, Eric; Schwarzbauer, Jan; Robert, Didier (Eds.) Environmental Chemistry : Green Chemistry and Pollutants in Ecosystems (2005)

In recent years, various mass spectrometry procedures has been developed for identifying bacteria. The accuracy and speed with which data can be obtained by Matrix-assisted laser Desorption/Ionization ... [more ▼]

In recent years, various mass spectrometry procedures has been developed for identifying bacteria. The accuracy and speed with which data can be obtained by Matrix-assisted laser Desorption/Ionization Time-of-flight Mass Spectrometry (MALDI-TOF-MS) make this an advantageous technique for environmental monitoring. However, minor variations in the sample preparation can influence the mass spectra significantly. In the present study, we have introduced a procedure to prepare bacteria by microextraction and we have optimized experimental parameters for rapid identification by MALDI-TOF-MS of whole bacterial cells isolated from environmental samples such as wastewater and soil. [less ▲]

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See detailDetection of BLV in frozen semen samples by PCR assay
Dus Santos, M.J.; Rodriguez, Sabrina ULg; Wigdorovitz, A. et al

in AIDS Research and Human Retroviruses (2007, April 23), 23(4), 651

Detection of BLV in frozen semen samples by PCR assay Dus Santos Maria Jose; Rodriguez Sabrina; Wigdorovitz Andrés; Trono Karina Instituto de Virología, CICVyA, INTA-Castelar. CC 77. Buenos Aires ... [more ▼]

Detection of BLV in frozen semen samples by PCR assay Dus Santos Maria Jose; Rodriguez Sabrina; Wigdorovitz Andrés; Trono Karina Instituto de Virología, CICVyA, INTA-Castelar. CC 77. Buenos Aires, Argentina. Detection of BLV in frozen semen samples by PCR assay María José Dus Santos, Sabrina Rodriguez, Andrés Wigdorovitz and Karina Trono. Instituto de Virología, CICVyA, INTA-Castelar. CC 77. Buenos Aires, Argentina mdussantos@cnia.inta.gov.ar The sanitary and economic impact of BLV infection is associated with the interference in the international movement of cattle and their germ plasm. Although experimental data support the improbability that semen from BLV-positive bulls could infect recipient cows, restriction for commercialization of semen from infected animals is still present. The objective of this work was to standardize a PCR assay to diagnose the presence of BLV genome and to describe the pattern of BLV detection in frozen semen samples. The developed methodology involves the amplification of an internal fragment of gag gene and posses a limit of detection of 60 viral particles. Additionally, a biological test in susceptible sheep was performed in order to evaluate the transmission of BLV genome by semen from seropositive animals. This data strongly suggest that semen from seropositive bulls that resulted negative by PCR can be used for artificial insemination (AI), accompanied by proper collection protocols. Frozen semen samples from 30 seropositive bulls were analyzed. It was possible to detect proviral DNA in 118 out of 545 samples. It was important to note that BLV genome detection occurred in several collections but in an alternated way with non detection periods. On the other hand, in 4 seropositive bulls, it was not possible to detect BLV genome in all the samples analyzed. The development of this PCR assay constitutes a valuable diagnostic tool to determine the BLV-free status of semen used for AI. Moreover, the results suggest that BLV could present an intermittent pattern of excretion. [less ▲]

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See detailDetection of BLV in frozen semen samples by PCR assay.
Rossich, L.; Gutiérrez, G.; Rodriguez, Sabrina ULg et al

Poster (2007, November 12)

Intermitent detection of BLV in frozen semen simples by PCR assay L. Rossich1, J. Gutierrez1, S. Rodriguez1, E. Lefebvre2, K. Trono1 and M. J. Dus Santos1 (1) Instituto de Virología, CICVyA, INTA-Castelar ... [more ▼]

Intermitent detection of BLV in frozen semen simples by PCR assay L. Rossich1, J. Gutierrez1, S. Rodriguez1, E. Lefebvre2, K. Trono1 and M. J. Dus Santos1 (1) Instituto de Virología, CICVyA, INTA-Castelar. CC 77. Buenos Aires, Argentina. (2) Centro de Inseminación Artificial La Elisa. Argentina. Introduction: The major route of transmission of BLV is horizontal by direct exposure to biological fluids contaminated with infected lymphocytes, mainly blood. Although viral antigens and proviral DNA has been identified in semen, milk and colostrums, natural transmission through these secretions has not been demonstrated. The sanitary and economic impact of BLV infection is associated with the interference in the international movement of cattle and their germ plasm. Although experimental data support the improbability that semen from BLV-positive bulls could infect recipient cows, restriction for commercialization of semen from infected animals is still present[1-6]. Since there is no vaccine or treatment available, eradication and control of the disease is exclusively based on early diagnostic and segregation of infected animals. In this context the specificity and sensitivity of the diagnostic test used is a critical point. We have previously developed a PCR assay with high sensitivity and specificity to detect BLV genome in frozen semen samples. The objective of this work was to study the detection of BLV in semen. Material & methods: Samples: Serum, whole blood and semen samples were obtained from CIALE (Artificial Insemination Centre La Elisa, Capitan Sarmiento, Buenos Aires, Argentina) Serology: The agar gel immunodifussion (AGID) test kit used to detect antibodies to BLV was produced by the Faculty of Veterinary, La Plata University, Argentina. An indirect ELISA using recombinant p24 as antigen was used to detect antibodies to BLV. This assay has been completely developed and standardized in the Institute of Virology, INTA-Castelar, Buenos Aires, Argentina. Detection of proviral DNA: DNA was extracted from PMBCs (purified from whole blood) and semen samples (fresh and straw). PCR assays that amplified a region of genes pol and gag and a nPCR that amplified a region of gene env were developed. Results: Two PCR assays were standardized for detecting BLV genome in semen and PMBC. The limit of detection of viral particles was assessed by the addition of purified pBLV344 (a plasmid containing the complete BLV genome, kindly provided by Dr. Willems, Faculté Universitaire des Sciences Agronomiques, Gembloux, Belgium) to DNA from semen or PMBCs of a seronegative bull. By gag-PCR and pol-PCR, it was possible to detect 60 viral particles, using bromide staining after electrophoresis separation of DNA. In order to increase analytical sensitivity, a nested-PCR was developed which amplified a region of env gene. The n-PCR was able to detected 6 viral particles. Assessment of the limit of detection was highly repetitive under the standardized conditions. Frozen semen samples from 30 seropositive bulls were remitted to the laboratory and analyzed in the period 2005-2007. It was possible to detect proviral DNA in 172 out of 862 samples. BLV genome detection occurred in several collections but in an alternated way with non detection periods. Fresh semen samples, straws and whole blood were also obtained from 5 seronegative and 5 seropositive bulls and tested together for the presence of BLV provirus. Results indicated that while detection of provirus was positive in PMBC from all seropositive bulls, detection of gag, pol and env genes in semen did not occurred in all the samples. Discussions & conclusions: The results obtained suggest that BLV could present an intermittent pattern of excretion. Further studies should be done to confirmed the results obtained and to establish why the presence of BLV provirus in semen is not constant. References : 1. Choi, K, et al. 2002. Vet Diagn Invest 14: 403-406. 2. Kaja, R. and Olson, C. 1982. Theriogenology 18: 107-112. 3. Miller, J. and Van Der Maaten, M. 1979. J Natl Cancer Inst 62; 425-428. 4. Monke, D. 1986. JAVMA 188 (8): 823-826. 5. Romero, C., et al. 1983. Tropical Animal Health and production. 15: 215-218. 6. Straub O. 1982. In: Fourth International Symposium of Bovine Leukosis. The Hague: Martinus Nijhoff Publishers: 299-308. [less ▲]

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