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See detailDetection of accase target-site resistant Alopecurus myosuroides huds (black-grass) in Belgian populations
Maréchal, Pierre-Yves ULg; Henriet, François; Bodson, Bernard ULg

in Communications in Agricultural and Applied Biological Sciences (2009, May 19)

Black-grass is a common grass weed, widely spread in Northern Europe and also in Belgium. For ages, it has been an increasing problem in industrial crops, especially winter cereals. The first case of ... [more ▼]

Black-grass is a common grass weed, widely spread in Northern Europe and also in Belgium. For ages, it has been an increasing problem in industrial crops, especially winter cereals. The first case of resistance in Belgium was reported in 1996 by Robert Bulcke (Eelen et al., 1996). Yet the resistance mechanism was not specified. Since then, no more information was published about the evolution Belgium, while research continued in the United Kingdom and in France. Moreover, during the last decade, progress in molecular biology allowed to highlight the mechanism of target-site resistance. A simple PCR method allows to detect the mutation conferring resistance to herbicide. After two years of resistance monitoring in Belgium, mostly in the Walloon part, some populations have been clearly identified as highly resistant to ACCase inhibitor. These populations have been tested by molecular biology so as to detect the single nucleotide polymorphism (SNP) involved in this case. The method employed was the Polymerase Chain Reaction Allele Specific Assays (PASA: Délye, 2002a) for the mutation Ile-1781-Leu that confers a target-site resistance to ACCase inhibitors. Those analyses were performed on plant material issued from bioassays, either in glasshouses or in Petri dishes. Leaves have been collected from plants which survived a fenoxaprop-P treatment applied in a glasshouse single dose assay. Seedlings from resistant populations grown in Petri dishes containing either fenoxaprop-P or cycloxydim provided the second type of sample. Ile1781 mutants were discovered within three populations. Each mutant plant was heterozygote. Five of those samples have been sequenced to confirm PASA results and everyone was matching. Moreover, they were all issued from Petri dishes containing cycloxydim, known to be unaffected by enhanced metabolism, confirming that theses populations are indeed target-site resistant. [less ▲]

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See detailDetection of Adverse Events in Administrative Data
Gillet, Pierre ULg; Kolh, Philippe ULg; Sermeus, W. et al

Report (2008)

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See detailDetection of aggregative behaviour in binary choice experiments
Brostaux, Yves ULg

Poster (2011, August 22)

Aggregative behaviour can be defined as a natural tendency for individuals to cluster in space into groups of varying sizes. In entomology, this behaviour can be detected in binary choice experiments ... [more ▼]

Aggregative behaviour can be defined as a natural tendency for individuals to cluster in space into groups of varying sizes. In entomology, this behaviour can be detected in binary choice experiments. Analysis of these experiments reveals specific characteristics (unequal counts between repetitions, dependence of individual choices) that make the traditional adjustment tests fail. We demonstrate that the use of generalized linear models can circumvent these pitfalls and deliver a reliable diagnosis on the social behaviour of the studied invertebrates. The strength of this behaviour can then be evaluated through the use of common correlation models. [less ▲]

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See detailDetection of Alternaria and Cladosporium DNA in nasal mucosa from dogs with idiopathic lymphoplasmacytic rhinitis
Mercier, Elise ULg; Peters, Iain; Billen, Frédéric ULg et al

in Proceedings of the 21st ECVIM-CA congress (2011, September 06)

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See detailDetection of Anaplasma phagocytophilum in Dermacentor reticulatus ticks
Wirtgen, Marc ULg; Nahayo, A.; Linden, Annick ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2011), 168(9), 248

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See detailDetection of Angiostrongylus vasorum in the bronchoalveolar lavage fluid or faeces of coughing and healthy dogs in Belgium.
Canonne-Guibert, Morgane ULg; Roels, Elodie ULg; Caron, Yannick ULg et al

in Proceedings of the 24th Ecvim Meeting, Mainz, Germany - 4-6 September 2014 (2014, September)

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See detailDetection of anthropogenic particles in fish stomachs: an isolation method adapted to identification by Raman spectroscopy
Collard, France ULg; Gilbert, Bernard ULg; Eppe, Gauthier ULg et al

in Archives of Environmental Contamination & Toxicology (2015), 69

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See detailDetection of anti-laminin antibodies in sera by latex agglutination.
Bernard, A. M.; Foidart, Jean-Michel ULg; Mahieu, P. et al

in Clinical Chemistry (1986), 32(8), 1468-72

We describe a latex particle agglutination assay for detecting circulating antibodies against laminin, a noncollagenous glycoprotein of basement membranes. Polystyrene latex particles on which laminin has ... [more ▼]

We describe a latex particle agglutination assay for detecting circulating antibodies against laminin, a noncollagenous glycoprotein of basement membranes. Polystyrene latex particles on which laminin has been adsorbed are incubated with serum for about 25 min at 42-45 degrees C. The agglutination is then measured by counting residual unagglutinated particles. Polyethylene glycol 6000 enhances the agglutination. The assay is fully automated, yielding results in about 45 min, for 50 samples per hour. Addition of purified laminin abolishes the agglutination of laminin-coated particles in practically all positive sera. The anti-laminin antibody titers obtained by this latex immunoassay and by radioimmunoassay correlated well in 161 sera from patients with suspected or established renal diseases. The agglutination assay more frequently gave positive results for cases of glomerulonephritis with linear deposits (20/22 cases) than for glomerulonephritis with granular deposits (7/68) or glomerulonephritis with no glomerular deposits (2/13). The finding of low anti-laminin antibody titers in sera from about 15% (34/230) of the healthy subjects suggests that these autoantibodies are pathogenic only in certain circumstances. [less ▲]

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See detailDetection of antibodies against Schmallenberg virus in wild boars, Belgium, 2010-2012
Desmecht, Daniel ULg; Garigliany, Mutien-Marie ULg; Beer, Martin et al

in Lecoq, Yves (Ed.) 31th Congress of the International Union of Game Biologists (2013, August 27)

In the summer/fall of 2011, a nonspecific febrile syndrome characterized by hyperthermia and drop in milk production with occasional reports of watery diarrhea and abortion was reported among dairy cows ... [more ▼]

In the summer/fall of 2011, a nonspecific febrile syndrome characterized by hyperthermia and drop in milk production with occasional reports of watery diarrhea and abortion was reported among dairy cows on farms in northwestern Europe. Further, in November 2011, an enzootic outbreak of malformed neonates emerged in several European countries, with stillbirth and birth at term of lambs, kids and calves with neurological signs or malformations of the head, spine, or limbs. Both syndromes were associated with the presence in the blood (adults) or in the central nervous system (newborns) of a new Shamonda/Sathuperi-like orthobunyavirus, provisionally named Schmallenberg virus (SBV) after the town in Germany where the first positive clinical samples were identified. Defining as precisely as possible the host range of the newcomer is a key point to predict the outcome of the emergence of SBV disease in Europe. In this respect, it must be pointed out that orthobunyaviruses infect more animal species than those in which the foetus is damaged. Recently, serological evidence for SBV infection in wild ruminant species (Cervus elaphus and Capreolus capreolus) was reported (Linden et al., 2012). In the present study, the objective was to seek after serological evidence of SBV infection among wild boars living in a geographical area where exposure to infected insect vectors was high in 2011, as judged from the very high seroprevalence reported among cattle in that region. About 700 animals were sampled during the 2010-2012 hunting seasons. All serum samples collected during the fall of 2010 were seronegative. On the contrary, apparent seroprevalence among wild boars in 2011 was ~27% and started to decline in 2012 (~11%). Acquired immunity against the new virus was thus already very high in the wild boar populations sampled in the fall 2011, suggesting that the new virus had quickly spread throughout the region since its emergence about 250 km northeast in the late summer 2011. The drop in seroprevalence recorded in 2012 suggests that the virus was no more circulating in the region. [less ▲]

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See detailDetection of antigenic heterogeneity in feline coronavirus nucleocapsid in feline pyogranulomatous meningoencephalitis.
Poncelet, Luc; Coppens, Angélique; Peeters, Dominique ULg et al

in Veterinary Pathology (2008), 45(2)

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See detailDetection of apple chlorotic leaf spot virus and apple stem grooving virus by RT-PCR and IC-RT-PCR.
Kummert, J.; Rufflard, Gladys ULg; Colinet, D. et al

in Mededelingen van de Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen (Rijksuniversiteit te Gent) (1995), 60(2a),

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See detailDetection Of Apple Chlorotic Leaf Spot Virus Using A 5 ' Nuclease Assay With A Fluorescent 3 ' Minor Groove Binder-Dna Probe
Vendrame, Marina; Kummert, Jean; Lepoivre, Philippe ULg et al

in Journal of Virological Methods (2002), 104(1), 99-106

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See detailDetection Of Apple Stem Grooving Virus In Dormant Apple Trees With Crude Extracts As Templates For One-Step Rt-Pcr
Marinho, Vla.; Kummert, J.; Rufflard, Gladys ULg et al

in Plant Disease (1998), 82(7),

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See detailDetection of Aspergillus spp. by PCR in Bronchoalveolar Lavage Fluid
Hayette, Marie-Pierre ULg; Vaira, Dolorès ULg; Suzin, Fabrice et al

in Journal of Clinical Microbiology (2001), 39(6), 2338-2340

The usefulness of a nested PCR assay for detection of Aspergillus sp. DNA was evaluated in 177 bronchoalveolar lavage (BAL) fluid specimens. This test was accurate both to diagnose culture-negative BAL ... [more ▼]

The usefulness of a nested PCR assay for detection of Aspergillus sp. DNA was evaluated in 177 bronchoalveolar lavage (BAL) fluid specimens. This test was accurate both to diagnose culture-negative BAL fluid specimens from patients with invasive pulmonary aspergillosis and to confirm culture-positive samples. However, it did not differentiate between infection and colonization. [less ▲]

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See detailDetection of Auroral Emissions from Callisto’s Magnetic Footprint at Jupiter
Clarke, J. T.; Wannawichian, S.; Hernandez, N. et al

Poster (2011, October)

HST observations of Jupiter’s aurora in a large campaign reveal several cases where the main oval emission appeared at unusually low latitudes, making it possible to search for the first time for auroral ... [more ▼]

HST observations of Jupiter’s aurora in a large campaign reveal several cases where the main oval emission appeared at unusually low latitudes, making it possible to search for the first time for auroral emissions from the magnetic footprint of Callisto without the overlapping bright emissions from the main oval. Several cases have been found where point-source emissions have now been detected from locations consistent with Callisto’s magnetic footprint on Jupiter at a brightness of ten’s of kilo- Rayleighs. These observations confirm that there is an electrodynamic interaction between Callisto and Jupiter’s magnetospheric environment that is similar to those at Io, Europa, and Ganymede, which all have auroral footprints. The properties of the emissions and a comparison with other observations and theoretical expectations will be presented in this paper. [less ▲]

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See detailDetection Of B-Cells And T-Cells, With Lectins Or Antibodies, In Healthy And Bovine Leukemia Virus-Infected Cattle
Fossum, C.; Burny, A.; Portetelle, Daniel ULg et al

in Veterinary Immunology and Immunopathology (1988), 18(3),

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See detailDetection of bacteria in sterile body fluids
CHRISTIAENS, Geneviève ULg; HAYETTE, Marie-Pierre ULg; De Mol, Patrick ULg

Poster (1998, October)

Purpose of the study: Development of a polymerase chain reaction (PCR) method for the detection of any bacterial DNA in synovial, cerebrospinal, pleural, and peritoneal fluids. Methods: Most of bacterial ... [more ▼]

Purpose of the study: Development of a polymerase chain reaction (PCR) method for the detection of any bacterial DNA in synovial, cerebrospinal, pleural, and peritoneal fluids. Methods: Most of bacterial species possess highly conserved, multicopy 16S ribosomal RNA genes, which can be hybridized with a single set of oligonucleotide primers. Samples were processed by an extraction protocol using proteinase K, and subjected to PCR amplification using two universal bacterial primers: RW01 and DG74; then the PCR products were detected by ethidium bromide gel electrophoresis. Study: Synovial, cerebrospinal, pleural and peritoneal fluids, obtained from 32 patients were analyzed by Gram stain, culture and PCR. Results: 1. The limit of detection, determined by analyzing successive dilutions of Staphylococcus aureus and Escherichia coli cultures in sterile water, was: 1.105 cfu/100µl. 2. We obtained 9 positive samples by culture: - 7 synovial fluids: S. agalactiae, S. viridans, coagulase negative Staphylococcus (2), S. epidermidis (2), and S. aureus. - 2 pleural fluids: S. pyogenes and Enterobacter aerogenes. All were PCR positive. 3. We tested 23 culture negative samples. All were negative by PCR. Conclusion: PCR presents some interesting features: 1. Only small amount of sample is necessary (100µl). 2. The duration of the test is shorter than 8 hours. 3. PCR provides similar or eventually greater sensitivity than culture and an excellent specificity (no false-positive and no false-negative results actually observed). [less ▲]

Detailed reference viewed: 75 (2 ULg)