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See detailDevelopment of an in vitro culture method of very immature embryos to assist wide interspecific hybridisation in Phaseolus.
Geerts, P.; Baudoin, Jean-Pierre ULg; Mergeai, Guy ULg

in 3rd European conference on grain legumes : Opportunities for high quality, healthy and added-value crops to meet European demands, 14-19 November 1998, Valladolid, Spain (1998)

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See detailDevelopment of an in vitro culture techniques adapted to Phaseolus polyanthus and P. vulgaris embryos.
Geerts, P.; Baudoin, Jean-Pierre ULg; Mergeai, Guy ULg

in Bean Improvement Cooperative (1998), 41

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See detailDevelopment of an in vitro endotoxin test for monoolein-water liquid crystalline gel for use as an implant
Ouédraogo, Moustapha; Semdé, Rasmané; Somé, Issa T. et al

in Tropical Journal of Pharmaceutical Research [=TJPR] (2009), 8(6), 501-508

Purpose : Drugs that are administered by parenteral route must be apyrogenic. The aim of this study was to develop an in vitro endotoxin test for liquid crytalline gels for use as implants, using a ... [more ▼]

Purpose : Drugs that are administered by parenteral route must be apyrogenic. The aim of this study was to develop an in vitro endotoxin test for liquid crytalline gels for use as implants, using a monoolein-water liquid crystalline gel as a model. Methods : The gel-clot technique was used. The gel was dissolved first in isopropyl myristate, and the endotoxins were extracted with water for bacterial endotoxin test. Tests for the labelled lysate sensitivity and interfering factors were performed to validate the developed method. The limit of detection of endotoxin in the gel was also determined. Results : The labelled lysate sensitivity was confirmed. It was not influenced by the presence of extracts from the gels. Endotoxins in the contaminated test gels were completely extracted. Endotoxin concentration in the tested gels was below the calculated threshold endotoxin level. Conclusion : A method to perform in vitro endotoxins test of liquid crystalline gels was successfully developed and validated. Application of the technique to gels currently being developed in our laboratories indicate that the gels were apyrogenic. [less ▲]

Detailed reference viewed: 34 (5 ULg)
See detailDevelopment of an in vitro model for mercury exposure in marine mammals
Dupont, Aurélie ULg; Bouquegneau, Jean-Marie ULg; Siebert, Ursula et al

Poster (2007, November 01)

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See detailDevelopment Of An In Vitro Pod Culture Technique For Young Pods Of Phaseolus Vulgaris L.
Geerts, P.; Sassi, K.; Mergeai, Guy ULg et al

in In Vitro Cellular & Developmental Biology-Plant (2000), 36(6),

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See detailDevelopment of an in vivo phosphorylation system in Escherichia coli
Goux, Marine ULg

Poster (2013, October)

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as zirconium-89 (89Zr, t½=78.4h) or gallium-68 (68Ga, t½=1.13h)[a ... [more ▼]

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as zirconium-89 (89Zr, t½=78.4h) or gallium-68 (68Ga, t½=1.13h)[a]. Despite their important role in the therapeutic world, antibodies have many disadvantages related to their structure. In addition to their limited stability, the presence of disulfide bridges and their post-translational modifications make their production difficult and expensive. Moreover, conjugation of chelating agent often occurs on lysines, which is non-regioselective and leads to a heterogeneous mixture of products. In addition, the long clearance of antibodies can be a problem to obtain a good contrast when they are used in imaging. To address these different limitations, we developed a chemistry-free chelating system consisting of a highly phosphorylatable peptide tag. A specific phosphorylation step, with the alpha subunit of the casein kinase II (CKIIα), generates a nanocluster of 4 phosphates that can interact strongly with metal ion like zirconium. This strategy has already demonstrated its powerfulness for the stable and specific anchoring of protein on zirconium phosphonate-based microarray[b]. We are now adapting the use of this labeling tag to the stereoselective chelation of radionuclides for PET imaging. To ensure an efficient targeting of the radionuclide, the tag was genetically fused to a Nanofitin®, a protein scaffold developed as an alternative to antibodies. Nanofitins share the advantages of being small (7KDa), cystein-free, easy to engineer, highly stable both to temperature and pH and expressed with high yield in Escherichia coli. As described previously, functionalizing the tag with a phosphate nanocluster is a key step to provide it with the capacity to specifically chelate metal ions such as zirconium or gallium. In early development the phosphorylation was realized in vitro. In order to save purification steps and decrease production costs, we developed an in vivo phosphorylation system consisting in the co-expression of a Nanofitin® fused to the phosphorylatable tag and the CKIIα. Effectiveness of the in vivo system was demonstrated together by electrophoretic mobility shift assay and staining with a specific phosphoprotein staining gel: ProQDiamond®. To further optimize and validate the system, the rate of phosphorylation is evaluated by an isoelectric focusing method. This technique is based on the fact that the pHi of the Nanofitin® decreases as the protein is phosphorylated. Such a technique allowed us to isolate the different phosphorylation states of the phospho-Nanofitin® (from 0 to 4 phospho-serine). In parallel to the development of the in vivo system, we are developing a phosphoprotein purification protocol by immobilized metal affinity chromatography, relying on the specific interaction of phosphate moeity with gallium(III) or iron(III). The use of a single tag for both the purification and the labeling will provide a much simpler and straightforward system. Finally this fusion system of phosphate nanocluster and a Nanofitin® will allows us to combine the advantages of an original single step regioselective labeling for both zirconium-89 and gallium-68, together with a highly stable targeting moiety. [a] Heuveling D. A. et al. (2011), Oral Oncology, 47, pp. 2–7 [b] Cinier M. et al. (2012), Journal of Biological Inorganic Chemistry, 17, pp. 399–407 [less ▲]

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See detailDEVELOPMENT OF AN INTEGRATED STRATEGY FOR CONTROLLING THE ALLERGEN ISSUE IN THE BELGIAN FOOD AND CATERING INDUSTRY - ALLERRISK
De Loose, Marc; Daeseleire, Else; Taverniers, Isabelle et al

Report (2009)

Detailed reference viewed: 33 (2 ULg)
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See detailDevelopment of an integrated tool for solar buildings design and renewal.
Andre, Philippe ULg; Nicolas, Jacques ULg

in Proceedings PLEA 96 International conference (1996, September)

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See detailDevelopment of an interface between a CFD software and a FE software for compartment fires
Tondini, Nicola; Franssen, Jean-Marc ULg

in Costruzioni Metalliche (2012), 3

The article presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a ... [more ▼]

The article presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a Finite Element (FE) software applied to structural systems. In particular, a weak coupling methodology used to simulate a fire exposed structure by modelling the fire development in the compartment, the heat penetration in the structure and the mechanical response is described. A practical application is also shown. [less ▲]

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See detailDevelopment of an interface between CFD and FE software
Tondini, Nicola; Vassart, Olivier; Franssen, Jean-Marc ULg

in Fontana, Mario; Frangi, Andrea; Knobloch, Markus (Eds.) Proceedings of the 7th International Conference on Structures in Fire (2012, June 06)

The paper presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a ... [more ▼]

The paper presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a Finite Element (FE) software applied to structural systems. In particular, a weak coupling approach used to simulate a fire exposed structure by modelling the fire development in the compartment, the heat penetration in the structure and the mechanical response is described. The advantages and the disadvantages of such a technique are highlighted compared to a full coupling that conversely takes into account all mutual interactions. The favourable aspect of computing the thermal response of the structure in the FE model in order to avoid modelling the structure in the CFD model is underlined, namely a sensitive reduction of computational demand. Finally, a practical application is presented. [less ▲]

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See detailDevelopment of an interspecific breeding programme in cotton assisted by RAPD markers
Mergeai, Guy ULg; Vroh Bi, I.; du Jardin, Patrick ULg et al

in Cotton biotechnology (1995)

Detailed reference viewed: 18 (4 ULg)
See detailDEVELOPMENT OF AN INTRAPERITONEAL IMPLANT FOR THE ENDOMETRIOSIS TREATMENT
Krier, Fabrice ULg; Nizet, Dominique; Riva, Raphaël ULg et al

Conference (2007)

Detailed reference viewed: 27 (6 ULg)
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See detailDEVELOPMENT OF AN INTRAPERITONEAL IMPLANT FOR THE ENDOMETRIOSIS TREATMENT
Krier, Fabrice ULg; Nizet, Dominique; Riva, Raphaël ULg et al

Poster (2008)

Detailed reference viewed: 47 (13 ULg)
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See detailDevelopment of an inverse method for identification of materials parameters in the single point incremental sheet forming process
Bouffioux, Chantal ULg; Henrard, Christophe ULg; Gu, Jun et al

in Tisza, M. (Ed.) Proceedings of the IDDRG 2007 International Conference (2007)

The purpose of this article is to develop an inverse method for adjusting the material parameters during single point incremental forming. The main idea consists in simulating tests performed on the same ... [more ▼]

The purpose of this article is to develop an inverse method for adjusting the material parameters during single point incremental forming. The main idea consists in simulating tests performed on the same machine as the one used for the process itself. This reduces the costs of the equipment since no specific and costly standard test equipment is needed. Moreover, it has the advantage that the material parameters are fitted for a heterogeneous stress and strain state occurring during the real process. Before using the inverse method, the numerical results must be compared with the experimental ones. Several boundary conditions will be tested. [less ▲]

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See detailDevelopment of an Open Source GIS Based Decision Support System for locating Wind Farms in Wallonia (Sourthern Belgium)
Lejeune, Philippe ULg; Gheysen, Thibaut ULg; Ducenne, Quentin et al

in Delvin, Ger (Ed.) Decision Support Systems , Advances in (2010)

The Walloon Region - Southern Belgium – aims at significantly increasing its wind-power electricity production capacity by the end of 2020. Such a development requires a comprehensive body of reliable ... [more ▼]

The Walloon Region - Southern Belgium – aims at significantly increasing its wind-power electricity production capacity by the end of 2020. Such a development requires a comprehensive body of reliable procedures pertaining to land use and landscape issues and subsequent controversy arising from wind farms located in densely populated areas. These procedures should enable experts and decision-makers to perform robust impact assessment analyses. To this end, an analytical framework based on GIS functionalities has been developed to evaluate and contrast various constraints against suitability criteria regarding building wind farms. This system can perform exhaustive spatial analyses over the whole region, i.e. 17,000 km², using a 10-m resolution in a raster mode. The approach is two-fold. Firstly, the framework allows an aggregation of 40 criteria related to both environmental and landscape constraints. The aggregation process is derived from Boolean algebra. It results into a 3-level constraint scale, i.e. exclusion, highly sensitive and sensitive. Secondly, the framework involves a weighted linear combination aggregation method where criteria are standardized on a [0,1] scale prior to aggregation. Different weights are required to translate criteria into a preference scale. In this process, the constraint map can be used as a mask for further suitability analysis. The geo-database and analysis functionalities were developed in an open source GIS GRASS environment. These functionalities are used to either import or export data from other GIS platforms, to update constraint and suitability maps, to perform an analysis of sensitivity with regard to criteria-defining parameters, and to perform exhaustive appraisals of wind farm projects. Grass GIS-tools seem to offer a good alternative to commercial GIS software for the development of such a Decision Support System as they ensure an easier access to stakeholders involved in the development of wind energy in Wallonia. [less ▲]

Detailed reference viewed: 190 (19 ULg)